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Effects of salinity acclimation on the proteome of the gilthead seabream (Sparus aurata) heart
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Abstracts / Comparative Biochemistry and Physiology, Part A 154 (2009) S31–S32
revealed that under hypoxic conditions cardiac output, stroke volume and end-diastolic volume increased, RBC concentration and -age composition. The network of blood vessels in the tail was found to be more complex as compared to the normoxic wildtypes. Parameters in environmentally influenced (e.g., hypoxia) phenotype and the new models revealed significant differences and call for the need of further investigation.
performance. This study was supported by the Russian Foundation for Basic Research (grant #09-04-01744). doi:10.1016/j.cbpa.2009.05.107
doi:10.1016/j.cbpa.2009.05.106
4. Effects of salinity acclimation on the proteome of the gilthead seabream (Sparus aurata) heart
3. New insights into the avian heart functioning
I. Varó (Instituto de Acuicultura de Torre de la Sal-SIC, Spain); J. Del Ramo, A. Torreblanca (University of Valencia, Spain); L. Cantero, M.M. Sánchez del Pino (Laboratorio de Proteómica of the CIPF, Spain)
V. Prosheva (Institute of Physiology, Syktyvkar, Russia); T. Shklyar, F. Blyakhman (Ural State Medical Academy, Ekaterinburg, Russia) It is known that mammal and avian hearts are four-chamber and their structure is mainly similar in representatives of these two classes of vertebrates. But there is a main difference among them on the macroscopic level — the avian heart contains a muscle valve (MV) between right atrium and ventricle that looks like a muscular plate. Noteworthy, this valve has neither tendinous threads nor papillary muscles. The effect of the MV on the avian heart function determination is not still clear. To understand the possible additional role of the MV in the normal avian right heart, we carried out electrophysiological and ultrasound investigations in the adult chickens Gallus domesticus. With use of conventional glass microelectrodes the conduction cells in the body of the MV were found. Electrophysiological characteristics of contractile cells of the MV were similar with those of the right ventricular free wall. Using intramural electrical potential mapping it was stated that the electrical activation of the MV occurs simultaneously with the main mass of myocardium of the right ventricle free wall. Ultrasound study showed the significant thickening of the MV at the systolic part of cardiac cycle. According to the right ventricle regional wall motion analysis, we found the MV contribution to the right ventricle ejection fraction (EF). Possibly, this contribution resulted in the increase of the right ventricle EF with average value of 90% versus 65% for the left ventricle. Based on the well-defined coordination between the MV electrical activation, MV kinematics and right ventricle regional function, we have concluded that the MV is an electromechanical structure of the avian right ventricle which maintains the heart pump
The gilthead seabream (Sparus aurata) is a valuable euryhaline species in the Mediterranean aquaculture, able of adapting to a considerable range of environmental salinity. Relatively little is known about the cardio-respiratory physiology of gilthead seabream in relation to the adaptations to salinity. Cardiac muscle function may be altered as consequence of ionic imbalances and loss or gain of tissue moisture and adjustments in the heart proteome to compensate for these effects are expected. To study differences in heart protein expression, fish were acclimated to high (37‰) and low salinity (18–20‰) for 5 months. Difference Gel Electrophoresis Technology (2D-DIGE) was used to study the effect salinity in heart protein profile. Resulting gels images were analyzed by DeCyderTM (V. 6.5) software and the statistical module EDA (V.1.0) was used for multivariate statistical analysed of data. The results showed significant changes in the expression of 39 proteins between high and low salinity group (p ≤ 0.05). Among these proteins, a total of 10 increased in abundance and 19 decreased in the group acclimated at high salinity. Mass spectrometry and database research is being performed to identify these differentially expressed proteins involved, presumably, into some homeostatic processes that would explain the gilthead seabream tolerance to a broad salinity range. This work was funded by project CTM 2006-14279-CO2-01/MAR MEC-FEDER. I. Varó was a recipient of a Ramón y Cajal contract MEC (Spain). The Laboratorio de Proteómica of the CIPF is a member of Proteored. doi:10.1016/j.cbpa.2009.05.108
Abstracts / Comparative Biochemistry and Physiology, Part A 154 (2009) S31–S32
revealed that under hypoxic conditions cardiac output, stroke volume and end-diastolic volume increased, RBC concentration and -age composition. The network of blood vessels in the tail was found to be more complex as compared to the normoxic wildtypes. Parameters in environmentally influenced (e.g., hypoxia) phenotype and the new models revealed significant differences and call for the need of further investigation.
performance. This study was supported by the Russian Foundation for Basic Research (grant #09-04-01744). doi:10.1016/j.cbpa.2009.05.107
doi:10.1016/j.cbpa.2009.05.106
4. Effects of salinity acclimation on the proteome of the gilthead seabream (Sparus aurata) heart
3. New insights into the avian heart functioning
I. Varó (Instituto de Acuicultura de Torre de la Sal-SIC, Spain); J. Del Ramo, A. Torreblanca (University of Valencia, Spain); L. Cantero, M.M. Sánchez del Pino (Laboratorio de Proteómica of the CIPF, Spain)
V. Prosheva (Institute of Physiology, Syktyvkar, Russia); T. Shklyar, F. Blyakhman (Ural State Medical Academy, Ekaterinburg, Russia) It is known that mammal and avian hearts are four-chamber and their structure is mainly similar in representatives of these two classes of vertebrates. But there is a main difference among them on the macroscopic level — the avian heart contains a muscle valve (MV) between right atrium and ventricle that looks like a muscular plate. Noteworthy, this valve has neither tendinous threads nor papillary muscles. The effect of the MV on the avian heart function determination is not still clear. To understand the possible additional role of the MV in the normal avian right heart, we carried out electrophysiological and ultrasound investigations in the adult chickens Gallus domesticus. With use of conventional glass microelectrodes the conduction cells in the body of the MV were found. Electrophysiological characteristics of contractile cells of the MV were similar with those of the right ventricular free wall. Using intramural electrical potential mapping it was stated that the electrical activation of the MV occurs simultaneously with the main mass of myocardium of the right ventricle free wall. Ultrasound study showed the significant thickening of the MV at the systolic part of cardiac cycle. According to the right ventricle regional wall motion analysis, we found the MV contribution to the right ventricle ejection fraction (EF). Possibly, this contribution resulted in the increase of the right ventricle EF with average value of 90% versus 65% for the left ventricle. Based on the well-defined coordination between the MV electrical activation, MV kinematics and right ventricle regional function, we have concluded that the MV is an electromechanical structure of the avian right ventricle which maintains the heart pump
The gilthead seabream (Sparus aurata) is a valuable euryhaline species in the Mediterranean aquaculture, able of adapting to a considerable range of environmental salinity. Relatively little is known about the cardio-respiratory physiology of gilthead seabream in relation to the adaptations to salinity. Cardiac muscle function may be altered as consequence of ionic imbalances and loss or gain of tissue moisture and adjustments in the heart proteome to compensate for these effects are expected. To study differences in heart protein expression, fish were acclimated to high (37‰) and low salinity (18–20‰) for 5 months. Difference Gel Electrophoresis Technology (2D-DIGE) was used to study the effect salinity in heart protein profile. Resulting gels images were analyzed by DeCyderTM (V. 6.5) software and the statistical module EDA (V.1.0) was used for multivariate statistical analysed of data. The results showed significant changes in the expression of 39 proteins between high and low salinity group (p ≤ 0.05). Among these proteins, a total of 10 increased in abundance and 19 decreased in the group acclimated at high salinity. Mass spectrometry and database research is being performed to identify these differentially expressed proteins involved, presumably, into some homeostatic processes that would explain the gilthead seabream tolerance to a broad salinity range. This work was funded by project CTM 2006-14279-CO2-01/MAR MEC-FEDER. I. Varó was a recipient of a Ramón y Cajal contract MEC (Spain). The Laboratorio de Proteómica of the CIPF is a member of Proteored. doi:10.1016/j.cbpa.2009.05.108