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Effects of Saskatoon Berry Powder on Monocyte Adhesion to Vascular Wall of Leptin Receptor-Deficient Diabetic Mice
Abstracts / Can J Diabetes 38 (2014) S29eS74
clamp, for assessing insulin sensitivity (M/I), and a 2-hour oral glucose tolerance test (OGTT) were performed before and after the experimental period. Results: The mean change in M/I (mgkg-1min-1pmol-1) was significantly improved in the polyphenol group (0.90.5) compared with the placebo group (e0.50.5) (p¼0.03). According to ANOVA with repeated measures for all time points of the OGTT, C-peptide increased in the placebo group, leading to a significant difference between the 2 groups (p¼0.002). Conclusion: Consumption of this combination of strawberry and cranberry extracts rich in polyphenols improved insulin sensitivity by 31% and prevented an increase in insulin secretion and could therefore represent a promising alternative approach to improve the metabolic profile of subjects at risk of type 2 diabetes.
195 Effects of Saskatoon Berry Powder on Monocyte Adhesion to Vascular Wall of Leptin Receptor-Deficient Diabetic Mice RUOZHI ZHAO, KHUONG LE, MOHAMMED MOGHADASIAN, TRUST BETA, GARRY SHEN Winnipeg, MB Hypothesis: Atherosclerotic cardiovascular complications are the leading cause of death in diabetic patients. Monocyte adhesion is an early event for atherogenesis. Previous studies demonstrated that dark-skin berries had cardiovascular protective effects. We hypothesize that SB powder may reduce monocyte adhesion in leptin receptor-deficient (db/db) diabetic mice. Methods: Wild-type and db/db mice were fed with chow or supplemented with SB powder. Anthocyanins in SB powder were identified using mass spectrometry. Mouse monocytes were incubated with mouse aorta. Monocyte adhesion was counted under microscopy. Inflammatory or metabolic markers in blood or tissue were analyzed using immunological or biochemical methods. Results: SB powder significantly reduced monocyte adhesion to aorta from diabetic db/db mice compared to regular chow. The increased monocyte adhesion to aorta was normalized in db/db mice treated with 5% of SB powder for 4 weeks. Increased contents of NAPDH oxidase-4, heat shock factor-1, monocyte chemotactic protein (MCP)-1, intracellular adhesion molecule (ICAM)-1, Pselectin, tumour necrosis factor-a, plasminogen activator inhibitor (PAI)-1 and urokinase plasminogen activator in aorta or heart apex, elevated plasma PAI-1 and MCP-1 were detected in db/db mice on chow compared to wild-type mice on the same diet. 5% SB powder inhibited the increases of inflammatory, fibrinolytic or stress regulators in aorta or heart apex of db/db mice. Monocyte adhesion positively correlated with blood glucose, cholesterol, body weight, heart MCP-1, PAI-1 or ICAM-1. Conclusion: The findings suggest that SB powder attenuated monocyte adhesion to aorta of db/db mice, which was potentially mediated through inhibiting the inflammatory, stress and/or fibrinolytic regulators.
S69
Subjects/methods: This is a cross-sectional analysis of 124 adults with T1D. Participants wore a motion sensor and completed a 3-day food record. The following CMR factors were assessed: body mass index, waist circumference, body composition (iDXA), glycated haemoglobin, lipids and blood pressure. Insulin resistance was estimated (estimated glucose disposal rate). Participants were classified according to the number of healthy lifestyle habits adopted (ranging from 0 to 3) within: regular physical activity (physical activity level 1.7), good diet quality (Canadian Healthy Eating Index score >80) and no-smoking status. Results: Eleven percent of the participants adopted all 3 healthy lifestyle habits. As the number of healthy lifestyle habits adopted increased, participants had significantly lower body mass index, waist circumference, body fat, total cholesterol, non-HDL-cholesterol, triglycerides and systolic blood pressure (p<0.05). In addition, a trend for lower estimated insulin resistance was observed (p¼0.06). For each increase of 1 healthy lifestyle habit, body mass index decreased by 1.9 kg/m2, waist circumference by 4.0 cm for men and 4.8 cm for women and trunk fat by 3.59% for men and 4.13% for women. Conclusions: These results suggest the importance of a healthy lifestyle among adults with T1D in order to control CMR factors.
197 Self-Reported Consumption of Low Glycemic Index Foods and Glycemic Control in Adults with Type 2 Diabetes HAYFORD AVEDZI, NONSIKELELO MATHE, CLARK MUNDT, JEFFREY JOHNSON, STEVE JOHNSON Edmonton, AB Replacing high glycemic index (GI) foods with low GI foods is recommended for people living with type 2 diabetes (T2D). This study examined associations between the number of self-reported days/week of consuming low GI foods and glycemic control in adults living with T2D. Adults participating in the Healthy Eating and Active Living for Diabetes in Primary Care Networks (HEALD-PCN) study completed a modified 55-item Block Food Frequency Questionnaire (FFQ) and answered 1 question about the number of days/week of consuming low GI foods at baseline. ANOVA was used to determine if the selfreported number of days/week of low GI food consumption (i.e. 0 days, 1e3 days, 4e5 days and 6e7 days) was associated with glycemic control. A total of 196 individuals (51% women), mean age 59.6 (8.5) years, and BMI, 33.6 (6.5) kg/m2 were included. Mean HbA1c was 6.8 (1.1)%. Mean energy was 1285.8 (459.7) kcals, GI and glycemic load (GL) were 51.7 (4.6) and 69.8 (28.1) respectively. Mean GI intake for 0 days was 52.8 (4.3), 1e3 days 50.9 (4.8), 4e5 days 51.0 (6.6) and 6e7 days was 51.6 (4.7). Overall, HbA1c, energy, GI and GL did not differ by self-reported number of days/week of low GI foods consumption (p>0.05).
198 196 Association Between Adoption of a Healthy Lifestyle and Cardiometabolic Profile of Adult Patients with Type 1 Diabetes CATHERINE LEROUX*, VÉRONIQUE GINGRAS, KATHERINE DESJARDINS, ANNE-SOPHIE BRAZEAU, SYLVIE OTTBRASCHI, IRENE STRYCHAR, RÉMI RABASA-LHORET Montreal, QC Background/objectives: Little is known about lifestyle habits of adults with type 1 diabetes (T1D) and their association with cardiometabolic risk (CMR) factors. The aim of the present study was to examine the prevalence of adults with T1D who adopted a healthy lifestyle and the relationship between a healthy lifestyle and the cardiometabolic profile.
Preventive Role of Green Tea Catechins Against Obesity and Related Disorders Especially Hyperglycemia and Hypercholesterolemia MUHAMMAD UMAIR ARSHAD, FARHAN SAEED Toronto, ON; Faisalabad, Punjab, Pakistan The core objective of current research is to explore nutraceutical worth of locally grown green tea variety (Qi-Men) against lifestylerelated disorders. For the purpose, green tea catechins and epigallocatechin gallate (EGCG) were isolated, characterized and the functional drinks containing these active components were assessed in experimental rats modelling. Based on diets, 4 studies were conducted; i.e. study I (normal diet), study II (high cholesterol diet), study III (high sucrose diet) and study IV (high cholesterol +
clamp, for assessing insulin sensitivity (M/I), and a 2-hour oral glucose tolerance test (OGTT) were performed before and after the experimental period. Results: The mean change in M/I (mgkg-1min-1pmol-1) was significantly improved in the polyphenol group (0.90.5) compared with the placebo group (e0.50.5) (p¼0.03). According to ANOVA with repeated measures for all time points of the OGTT, C-peptide increased in the placebo group, leading to a significant difference between the 2 groups (p¼0.002). Conclusion: Consumption of this combination of strawberry and cranberry extracts rich in polyphenols improved insulin sensitivity by 31% and prevented an increase in insulin secretion and could therefore represent a promising alternative approach to improve the metabolic profile of subjects at risk of type 2 diabetes.
195 Effects of Saskatoon Berry Powder on Monocyte Adhesion to Vascular Wall of Leptin Receptor-Deficient Diabetic Mice RUOZHI ZHAO, KHUONG LE, MOHAMMED MOGHADASIAN, TRUST BETA, GARRY SHEN Winnipeg, MB Hypothesis: Atherosclerotic cardiovascular complications are the leading cause of death in diabetic patients. Monocyte adhesion is an early event for atherogenesis. Previous studies demonstrated that dark-skin berries had cardiovascular protective effects. We hypothesize that SB powder may reduce monocyte adhesion in leptin receptor-deficient (db/db) diabetic mice. Methods: Wild-type and db/db mice were fed with chow or supplemented with SB powder. Anthocyanins in SB powder were identified using mass spectrometry. Mouse monocytes were incubated with mouse aorta. Monocyte adhesion was counted under microscopy. Inflammatory or metabolic markers in blood or tissue were analyzed using immunological or biochemical methods. Results: SB powder significantly reduced monocyte adhesion to aorta from diabetic db/db mice compared to regular chow. The increased monocyte adhesion to aorta was normalized in db/db mice treated with 5% of SB powder for 4 weeks. Increased contents of NAPDH oxidase-4, heat shock factor-1, monocyte chemotactic protein (MCP)-1, intracellular adhesion molecule (ICAM)-1, Pselectin, tumour necrosis factor-a, plasminogen activator inhibitor (PAI)-1 and urokinase plasminogen activator in aorta or heart apex, elevated plasma PAI-1 and MCP-1 were detected in db/db mice on chow compared to wild-type mice on the same diet. 5% SB powder inhibited the increases of inflammatory, fibrinolytic or stress regulators in aorta or heart apex of db/db mice. Monocyte adhesion positively correlated with blood glucose, cholesterol, body weight, heart MCP-1, PAI-1 or ICAM-1. Conclusion: The findings suggest that SB powder attenuated monocyte adhesion to aorta of db/db mice, which was potentially mediated through inhibiting the inflammatory, stress and/or fibrinolytic regulators.
S69
Subjects/methods: This is a cross-sectional analysis of 124 adults with T1D. Participants wore a motion sensor and completed a 3-day food record. The following CMR factors were assessed: body mass index, waist circumference, body composition (iDXA), glycated haemoglobin, lipids and blood pressure. Insulin resistance was estimated (estimated glucose disposal rate). Participants were classified according to the number of healthy lifestyle habits adopted (ranging from 0 to 3) within: regular physical activity (physical activity level 1.7), good diet quality (Canadian Healthy Eating Index score >80) and no-smoking status. Results: Eleven percent of the participants adopted all 3 healthy lifestyle habits. As the number of healthy lifestyle habits adopted increased, participants had significantly lower body mass index, waist circumference, body fat, total cholesterol, non-HDL-cholesterol, triglycerides and systolic blood pressure (p<0.05). In addition, a trend for lower estimated insulin resistance was observed (p¼0.06). For each increase of 1 healthy lifestyle habit, body mass index decreased by 1.9 kg/m2, waist circumference by 4.0 cm for men and 4.8 cm for women and trunk fat by 3.59% for men and 4.13% for women. Conclusions: These results suggest the importance of a healthy lifestyle among adults with T1D in order to control CMR factors.
197 Self-Reported Consumption of Low Glycemic Index Foods and Glycemic Control in Adults with Type 2 Diabetes HAYFORD AVEDZI, NONSIKELELO MATHE, CLARK MUNDT, JEFFREY JOHNSON, STEVE JOHNSON Edmonton, AB Replacing high glycemic index (GI) foods with low GI foods is recommended for people living with type 2 diabetes (T2D). This study examined associations between the number of self-reported days/week of consuming low GI foods and glycemic control in adults living with T2D. Adults participating in the Healthy Eating and Active Living for Diabetes in Primary Care Networks (HEALD-PCN) study completed a modified 55-item Block Food Frequency Questionnaire (FFQ) and answered 1 question about the number of days/week of consuming low GI foods at baseline. ANOVA was used to determine if the selfreported number of days/week of low GI food consumption (i.e. 0 days, 1e3 days, 4e5 days and 6e7 days) was associated with glycemic control. A total of 196 individuals (51% women), mean age 59.6 (8.5) years, and BMI, 33.6 (6.5) kg/m2 were included. Mean HbA1c was 6.8 (1.1)%. Mean energy was 1285.8 (459.7) kcals, GI and glycemic load (GL) were 51.7 (4.6) and 69.8 (28.1) respectively. Mean GI intake for 0 days was 52.8 (4.3), 1e3 days 50.9 (4.8), 4e5 days 51.0 (6.6) and 6e7 days was 51.6 (4.7). Overall, HbA1c, energy, GI and GL did not differ by self-reported number of days/week of low GI foods consumption (p>0.05).
198 196 Association Between Adoption of a Healthy Lifestyle and Cardiometabolic Profile of Adult Patients with Type 1 Diabetes CATHERINE LEROUX*, VÉRONIQUE GINGRAS, KATHERINE DESJARDINS, ANNE-SOPHIE BRAZEAU, SYLVIE OTTBRASCHI, IRENE STRYCHAR, RÉMI RABASA-LHORET Montreal, QC Background/objectives: Little is known about lifestyle habits of adults with type 1 diabetes (T1D) and their association with cardiometabolic risk (CMR) factors. The aim of the present study was to examine the prevalence of adults with T1D who adopted a healthy lifestyle and the relationship between a healthy lifestyle and the cardiometabolic profile.
Preventive Role of Green Tea Catechins Against Obesity and Related Disorders Especially Hyperglycemia and Hypercholesterolemia MUHAMMAD UMAIR ARSHAD, FARHAN SAEED Toronto, ON; Faisalabad, Punjab, Pakistan The core objective of current research is to explore nutraceutical worth of locally grown green tea variety (Qi-Men) against lifestylerelated disorders. For the purpose, green tea catechins and epigallocatechin gallate (EGCG) were isolated, characterized and the functional drinks containing these active components were assessed in experimental rats modelling. Based on diets, 4 studies were conducted; i.e. study I (normal diet), study II (high cholesterol diet), study III (high sucrose diet) and study IV (high cholesterol +