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Effects of Simulated Microgravital Environment on Lung Cancer Cells
Biotechnology SESSION TITLE: Biotechnology SESSION TYPE: Original Investigation Poster PRESENTED ON: Wednesday, October 26, 2016 at 01:30 PM - 02:30 PM
Effects of Simulated Microgravital Environment on Lung Cancer Cells Jae Ho Chung* Eunjue Yi Chi Bum Ahn Sungho Lee; and Kwang Taik Kim Korea University Medical College, Korea University Anam Hospital, Seoul, Korea (the Republic of) PURPOSE: To examine the effects of simulated microgravity on metastatic ability of the different cell lines of human lung adenocarcinoma and squamous cell carcinoma. METHODS: Human lung cancer cell lines of adenocarcinoma(A549) and squamous cell carcinoma(H1703) were cultured and maintained in a humidified incubator at 37 C under a 5% CO2 atmosphere. A clinostat system(3D clinostat ver.2) was used for creating simulated microgravity environment and cells were plated into a membrane cell culture dish. The cells adhered to the flask were fixed carefully at the rotating panel of clinostat system, which was placed in the humidified incubator at 37 C under a 5% CO2 atmosphere. The clinostat was continuously rotated at 5 rpm for 36 hours. The control group was cultured in the same way as the experimental group with the exception of clinorotation. In order to analyze the effect of microgravity on proliferation and migration abilities of lung cancer cells, cell proliferation assay, and wound healing scratch assay were performed. And also, to analyze the microgravital effect on the expression levels of biomarkers related to cell migration and invasion, real-time PCR assay and western blot assay of MMP2, MMP9, TIMP-1, TIMP-2 were performed.
BIOTECHNOLOGY
RESULTS: The effect of simulated microgravity influenced differently on the different cell lines of lung cancer cells. The microgravital environment did not significantly influence the cell proliferation of A549 cell line of adenocarcinoma, but it inhibited the proliferation of H1703 cell line of squamous cell carcinoma. The migratory ability of both A549 and H1703 cell lines were increased after exposure to microgravity when compared to normal gravity. But, the results of molecular changes in biomarkers after exposure to microgravity seems to be rather preliminary. CONCLUSIONS: We could carefully suggest that the simulated microgravity influenced differently on the different cells lines of lung cancer. And, further studies may be needed to achieve a more thorough explanation of microgravital effect of the lung cancer cells. CLINICAL IMPLICATIONS: Despite the improvements in diagnosis, surgical techniques, and local and systemic adjuvant therapies, lung cancer still remains a major worldwide health problem. And, most deaths from cancer result from the progressive growth of metastases. Recently, the effects of microgravity on cell morphology, structure, functions and their mechanisms have been widely studied. And, considering the limitations of performing experiments in real microgravital environment on Earth, random positioning machines named “clinostat” have been developed in order to create “simulated microgravity” environment. Although there were previous reports on the biological effects of simulated microgravity on different types of cancer cells, the effects of simulated microgravity on the process of human lung cancer metastasis has not been revealed sufficiently. Considering the many different subtypes and different cell lines in human lung cancer cells, our study may suggest informations on how cancer cells’ metastatic ability might change during the microgravital environment. DISCLOSURE: The following authors have nothing to disclose: Jae Ho Chung, Eunjue Yi, Chi Bum Ahn, Sungho Lee, Kwang Taik Kim No Product/Research Disclosure Information DOI:
http://dx.doi.org/10.1016/j.chest.2016.08.032
Copyright ª 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.
26A
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150#4S CHEST OCTOBER 2016
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Effects of Simulated Microgravital Environment on Lung Cancer Cells Jae Ho Chung* Eunjue Yi Chi Bum Ahn Sungho Lee; and Kwang Taik Kim Korea University Medical College, Korea University Anam Hospital, Seoul, Korea (the Republic of) PURPOSE: To examine the effects of simulated microgravity on metastatic ability of the different cell lines of human lung adenocarcinoma and squamous cell carcinoma. METHODS: Human lung cancer cell lines of adenocarcinoma(A549) and squamous cell carcinoma(H1703) were cultured and maintained in a humidified incubator at 37 C under a 5% CO2 atmosphere. A clinostat system(3D clinostat ver.2) was used for creating simulated microgravity environment and cells were plated into a membrane cell culture dish. The cells adhered to the flask were fixed carefully at the rotating panel of clinostat system, which was placed in the humidified incubator at 37 C under a 5% CO2 atmosphere. The clinostat was continuously rotated at 5 rpm for 36 hours. The control group was cultured in the same way as the experimental group with the exception of clinorotation. In order to analyze the effect of microgravity on proliferation and migration abilities of lung cancer cells, cell proliferation assay, and wound healing scratch assay were performed. And also, to analyze the microgravital effect on the expression levels of biomarkers related to cell migration and invasion, real-time PCR assay and western blot assay of MMP2, MMP9, TIMP-1, TIMP-2 were performed.
BIOTECHNOLOGY
RESULTS: The effect of simulated microgravity influenced differently on the different cell lines of lung cancer cells. The microgravital environment did not significantly influence the cell proliferation of A549 cell line of adenocarcinoma, but it inhibited the proliferation of H1703 cell line of squamous cell carcinoma. The migratory ability of both A549 and H1703 cell lines were increased after exposure to microgravity when compared to normal gravity. But, the results of molecular changes in biomarkers after exposure to microgravity seems to be rather preliminary. CONCLUSIONS: We could carefully suggest that the simulated microgravity influenced differently on the different cells lines of lung cancer. And, further studies may be needed to achieve a more thorough explanation of microgravital effect of the lung cancer cells. CLINICAL IMPLICATIONS: Despite the improvements in diagnosis, surgical techniques, and local and systemic adjuvant therapies, lung cancer still remains a major worldwide health problem. And, most deaths from cancer result from the progressive growth of metastases. Recently, the effects of microgravity on cell morphology, structure, functions and their mechanisms have been widely studied. And, considering the limitations of performing experiments in real microgravital environment on Earth, random positioning machines named “clinostat” have been developed in order to create “simulated microgravity” environment. Although there were previous reports on the biological effects of simulated microgravity on different types of cancer cells, the effects of simulated microgravity on the process of human lung cancer metastasis has not been revealed sufficiently. Considering the many different subtypes and different cell lines in human lung cancer cells, our study may suggest informations on how cancer cells’ metastatic ability might change during the microgravital environment. DISCLOSURE: The following authors have nothing to disclose: Jae Ho Chung, Eunjue Yi, Chi Bum Ahn, Sungho Lee, Kwang Taik Kim No Product/Research Disclosure Information DOI:
http://dx.doi.org/10.1016/j.chest.2016.08.032
Copyright ª 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.
26A
[
150#4S CHEST OCTOBER 2016
]