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Effects of the c-H-RAS oncogene on colon cancer cell lines
Cell Biology International
Reports, Vol. 14, Abstracts Supplement
CO-EXPRESSION OF c-FOS AND IFN GENES BY OVINE TROPHECTODEAM. Francoke Xavier, Michel Guillomot, Madia Charlier, Jacques Martal, Pierre Gayer). Unite Endocrinologie de I’Embryon and (“)UnitB Endocrinologie Molkulaire, INRA, 78350 Jouy-en-Josas, France.
P41
Expression of the proto-oncogene c-fos by ovine conceptus was analyzed by Northern and slot blots in relation with the expression of an interferon a-II (IFN a-II), named ovine Trophoblastin Protein (oTP). This IFN is produced only by the trophectoderm during the peri-implantation period. The proto-oncogene c-fos was expressed by extra-embryonic membranes (trophectoderm and allantois) and embryo. In the trophectoderm, the amount of c-fos mRNA was low at day 14, increased progressively to reach a maximum by days 16-17, whereas the level of oTP mRNA was maximum by days 14 -15 and declined progressively thereafter. By day 21, complete extinction of oTP and afos genes was observed. Thus, the proto-oncogene c-fos is expressed in the tissue trophectoderm when : - (1) this extra-embryonic differentiates during the implantation process, - (2) the amount of oTP mRNA declines. This decrease is due to the arrest of oTP expression in trophoblastic cells which are in contact with the uterine epithelium. During the impiantation process, non-adherent cells expressing and producing oTP and adherent cells no longer expressing oTP are present in the trophectoderm. Experiments are in progress to determine whether : (1) c-fos and IFN (oTP) genes are cc-expressed in the same trophoblastic cells, (2) IFN is involved in the regulation of c-fos gene expression in ovine trophectoderm.
P43
ANTIBODIES AGAINST hsp72/73 INS IN PATIENT WITH BREAST
P42
EFFECTS OF RETROVIRAL ONCOGENES ON MYOGENESIS
IN VITRO Germana Falcone, M.Ctistina Gauzzi, Franc0 Tat6* and Siefano AlemiL Istituto di Bioiogia Cellulare, CNR, and *Universiti “La Sapienza”, Roma, Italy. Myogenic cells expressing exogenous oncogenes represent a valuable model to address questions about the influence of genetic and epigenetic messages in myogenesis and their interplay with oncogenes, growth control and expression of differentiated functions. Quail myogenic cells infected with temperature.sensitive mutants of Rous sarcoma vilus exhibit a temperature-dependent transformation and block of differentiation. When the cells are allowed to differentiate at the restrictive temperature and then shifted back to the permissive temperature, a sharp reduction in the accumulation of muscle specific mRNAs and protein products is observed, following reactivation of tbe transforming protein pp6Ov-S’c Studies on transcription of endogenous muscle specific genes and transfected CAT reporter gene under the control of muscle specific promoters, at the different temperatures, suggests that the oncogeneexerts its control mainly at the tmncriptional level. Myoblasts of fhe murine cell line C2 can also be efliciently transformed in v&o by infection with retroviruses coding for the cytoplasmic oncogenesV-SK, v-ras and polyoma middle T, as assessedby the acquisition of anchorage-independence for growth and block of fusion and biochemical differentiation.‘There is increasing evidence for the existence of muscle regulatory genes, such as MyoD and myogenin, that govern and influence muscle development and myogenic differentiation in vitro. Data on the expression of MyoD and myogenin in clonal cell lines derived from both primary myoblasts and C2 cells transformed by v-src will be presented. This work was supported by grants from AIRC, Fondazione Cenci-Bolognetti and CNR-PF-Biotecnologie.
PROTECANCER-
Ingela Kindas-Miigge, I$se Frijhlich, Inst. of Tumorbiol.Univ.ofYienna, Austria A specific interaction between the heat shock 7okDa protein family(hsp72/hsc73), also called stress proteins, and the cellular tumor antigen p53 has been demonstrated.These data suggest that the p53/hsp7o complex could have some functional significance in transformed cells. The host pro tein ~53 has been shown to be immunogenic and autoantibodies against pT3 have been found in sera from patients with mammary carcinoma. In this study, sera obtained from patients with breast cancer were analysed for antibodies against the hsp7o class proteins and the cellular oncogene ~53. Hsp 70 purified from HT-1080 cells and proteins, immunoprecipitated from SV(Bo), were P53, used as antigen sources in immunoblotting experiments. About 20% of the sera recognized hsp72 and/or hsp73 proteins. However, only some of these sera contained autoantibodies against the oncogene proresults implicate an imduct ~53. These mune response to self stress protein determinants in such a way that could help to eliminate own cells which are transformed, as shown above, or otherwise stressed.
89
1990
EFFECW OF THE C-H-RAS ONCOGENE ON COLON CANCER CELL LINES Johan E. De Vrie~, Peter M. Mocrkerk, Leon GA.
of differentiationof the cell line used To test these statements
for their mctastatic behaviour after orthotopic xeno&ting or injection into the ml eiradatioo. Prclimioq rcSuk6 however show 110dramatie d&es in growth eharachshca mayhe due to the fact that thwe is no incrsase. in RAS P2i level in the tnWfec&d~e~line~wi%ichdoha~~anenhau~RASmRNA expression.
Reports, Vol. 14, Abstracts Supplement
CO-EXPRESSION OF c-FOS AND IFN GENES BY OVINE TROPHECTODEAM. Francoke Xavier, Michel Guillomot, Madia Charlier, Jacques Martal, Pierre Gayer). Unite Endocrinologie de I’Embryon and (“)UnitB Endocrinologie Molkulaire, INRA, 78350 Jouy-en-Josas, France.
P41
Expression of the proto-oncogene c-fos by ovine conceptus was analyzed by Northern and slot blots in relation with the expression of an interferon a-II (IFN a-II), named ovine Trophoblastin Protein (oTP). This IFN is produced only by the trophectoderm during the peri-implantation period. The proto-oncogene c-fos was expressed by extra-embryonic membranes (trophectoderm and allantois) and embryo. In the trophectoderm, the amount of c-fos mRNA was low at day 14, increased progressively to reach a maximum by days 16-17, whereas the level of oTP mRNA was maximum by days 14 -15 and declined progressively thereafter. By day 21, complete extinction of oTP and afos genes was observed. Thus, the proto-oncogene c-fos is expressed in the tissue trophectoderm when : - (1) this extra-embryonic differentiates during the implantation process, - (2) the amount of oTP mRNA declines. This decrease is due to the arrest of oTP expression in trophoblastic cells which are in contact with the uterine epithelium. During the impiantation process, non-adherent cells expressing and producing oTP and adherent cells no longer expressing oTP are present in the trophectoderm. Experiments are in progress to determine whether : (1) c-fos and IFN (oTP) genes are cc-expressed in the same trophoblastic cells, (2) IFN is involved in the regulation of c-fos gene expression in ovine trophectoderm.
P43
ANTIBODIES AGAINST hsp72/73 INS IN PATIENT WITH BREAST
P42
EFFECTS OF RETROVIRAL ONCOGENES ON MYOGENESIS
IN VITRO Germana Falcone, M.Ctistina Gauzzi, Franc0 Tat6* and Siefano AlemiL Istituto di Bioiogia Cellulare, CNR, and *Universiti “La Sapienza”, Roma, Italy. Myogenic cells expressing exogenous oncogenes represent a valuable model to address questions about the influence of genetic and epigenetic messages in myogenesis and their interplay with oncogenes, growth control and expression of differentiated functions. Quail myogenic cells infected with temperature.sensitive mutants of Rous sarcoma vilus exhibit a temperature-dependent transformation and block of differentiation. When the cells are allowed to differentiate at the restrictive temperature and then shifted back to the permissive temperature, a sharp reduction in the accumulation of muscle specific mRNAs and protein products is observed, following reactivation of tbe transforming protein pp6Ov-S’c Studies on transcription of endogenous muscle specific genes and transfected CAT reporter gene under the control of muscle specific promoters, at the different temperatures, suggests that the oncogeneexerts its control mainly at the tmncriptional level. Myoblasts of fhe murine cell line C2 can also be efliciently transformed in v&o by infection with retroviruses coding for the cytoplasmic oncogenesV-SK, v-ras and polyoma middle T, as assessedby the acquisition of anchorage-independence for growth and block of fusion and biochemical differentiation.‘There is increasing evidence for the existence of muscle regulatory genes, such as MyoD and myogenin, that govern and influence muscle development and myogenic differentiation in vitro. Data on the expression of MyoD and myogenin in clonal cell lines derived from both primary myoblasts and C2 cells transformed by v-src will be presented. This work was supported by grants from AIRC, Fondazione Cenci-Bolognetti and CNR-PF-Biotecnologie.
PROTECANCER-
Ingela Kindas-Miigge, I$se Frijhlich, Inst. of Tumorbiol.Univ.ofYienna, Austria A specific interaction between the heat shock 7okDa protein family(hsp72/hsc73), also called stress proteins, and the cellular tumor antigen p53 has been demonstrated.These data suggest that the p53/hsp7o complex could have some functional significance in transformed cells. The host pro tein ~53 has been shown to be immunogenic and autoantibodies against pT3 have been found in sera from patients with mammary carcinoma. In this study, sera obtained from patients with breast cancer were analysed for antibodies against the hsp7o class proteins and the cellular oncogene ~53. Hsp 70 purified from HT-1080 cells and proteins, immunoprecipitated from SV(Bo), were P53, used as antigen sources in immunoblotting experiments. About 20% of the sera recognized hsp72 and/or hsp73 proteins. However, only some of these sera contained autoantibodies against the oncogene proresults implicate an imduct ~53. These mune response to self stress protein determinants in such a way that could help to eliminate own cells which are transformed, as shown above, or otherwise stressed.
89
1990
EFFECW OF THE C-H-RAS ONCOGENE ON COLON CANCER CELL LINES Johan E. De Vrie~, Peter M. Mocrkerk, Leon GA.
of differentiationof the cell line used To test these statements
for their mctastatic behaviour after orthotopic xeno&ting or injection into the ml eiradatioo. Prclimioq rcSuk6 however show 110dramatie d&es in growth eharachshca mayhe due to the fact that thwe is no incrsase. in RAS P2i level in the tnWfec&d~e~line~wi%ichdoha~~anenhau~RASmRNA expression.