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Electricity from biogas. Integrated plan for Tucuman in north west Argentina
S148
Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576
tion enzyme, microbial cells and carbohydrate from outside. The major microbial cells in WBFB were isolated and identified. The variations in compositions of WBFB, ethanol, acetaldehyde and acetic acid including CFU values with stock time were investigated. The effects of process conditions, temperature, working volume and reaction mode e.g. static, shaking and agitation on the production of bio-ethanol were studied. The higher working volume of the medium in a given reactor caused an evident positive effect on the bio-ethanol production. The increase in process temperature in agitation conditions shortened the time required for bio-ethanol production. From this whole study it was found that the use of WBFB is a cheap and suitable source for ethanol production. doi:10.1016/j.jbiotec.2010.08.382 [P-B.30] Ethanol production from sweet sorghum juice in repeatedbatch fermentation by Saccharomyces cerevisiae immobilized on corncob Pattana Laopaiboon ∗ , Lakkana Laopaiboon Khon Kaen University, Thailand Keywords: Corncob; Immobilized yeasts; Repeated batch ethanol fermentation; Sweet sorghum juice; Saccharomyces cerevisiae Sweet sorghum has been promised as a large scale energy crop because its stalks contain high fermentable sugar and it can be cultivated in nearly all temperatures and tropical climate areas Rajvanshi and Nimbkar, 2005. In this study, batch and repeated batch ethanol fermentation from sweet sorghum juice containing total soluble solids of 24 o Brix by Saccharomyces cerevisiae TISTR 5048 and S. cerevisiae NP 01 immobilized on a low cost support material, corncob, were investigated. S. cerevisiae TISTR 5048 immobilized on 6 × 6 × 6-mm3 corncob gave higher ethanol production than those on 12 × 12 × 12-mm3 corncob in terms of ethanol concentration (P), yield (Yp/s ) and productivity (Qp ) with the values of 100.69 g l−1 , 0.45 and 2.10 g l−1 h−1 , respectively. In repeated batch ethanol fermentation by the yeasts immobilized on the 6 × 6 × 6-mm3 corncob could be used at least eight successive cycles without a significant decrease in ethanol production. The average P, Yp/s and Qp of the repeated-batch fermentation in the eight cycles were 93.69 ± 5.03 g l−1 , 0.45 ± 0.01 and 1.95 ± 0.10 g l−1 h−1 , respectively at the fermentation time (each batch) of 48 h. The repeated batch ethanol fermentation by S. cerevisiae NP 01 immobilized on the 6 × 6 × 6-mm3 corncob was also carried out for eight cycles. T-test method showed that P, Yp/s and Qp of the two yeast strains were not significantly different (P > 0.05). The ethanol production efficiencies in the repeated batch fermentation by S. cerevisiae TISTR 5048 immobilized on the 6 × 6 × 6-mm3 corncob were compared with those of the yeast immobilized on 3-mm calcium alginate bead (a widely used support material) Laopaiboon and Laopaiboon, 2006. The results showed that the yeast immobilized on the corncob gave significantly higher P, Yp/s and Qp than those immobilized on calcium alginate (P < 0.05). The results obtained from this study indicate that corncob is a suitable support material to immobilize the yeasts for ethanol production.
References Rajvanshi, A.K. and Nimbkar, N. (2005) Sweet sorghum R&D at the Nimbkar Agricultural Research Institute (NARI). http://nariphaltan.virtualave.net/sorghum.htm. Cited 5 Oct 2005.
Laopaiboon, L., Laopaiboon, P., 2006. Repeated-batch ethanol production from sweet sorghum juice by immobilized yeasts. In: The 8th Agro-Industrial Conference: Food Innovation, June 15-16, 2006, Bangkok, Thailand.
doi:10.1016/j.jbiotec.2010.08.383 [P-B.31] Electricity from biogas. Integrated plan for Tucuman in north west Argentina F. Sineriz 1,2 1
PROIMI Biotech-CONICET, Argentina Microbiology, Fac de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Argentina Keywords: Biofuels; Anaerobic treatment; Citrus processing plants; Distillery waste 2
The North West province of Tucumán, Argentina, is the first world exporter of fresh lemon fruits and one important producer of products of the lemon industrialization. Altogether there are about 35,000 ha dedicated to the production, which is handled by seven processing plants. Under new legislation wastewater treatment is mandatory. Most of the processing plants have chosen anaerobic systems. Actually, there is already one anaerobic system in Citrusvil (one of the two largest lemon processing plants) producing 2900 Nm3 /hour. Another plant for Citricola San Miguel is almost finished and due to start operating during the year. Two another smaller processing plants are currently building 1,000 m3 digesters under our supervision. The combined biogas production arising from anaerobic waste water treatment of the processing plants could reach a total of about 10,000 cubic meters of gas daily. Tucuman also concentrates half of the country production of bioethanol, coming from about 150.000 ha of sugar cane, elaborated in 15 sugar mills. On the distillery front, the production is close to 200,000 m3 ethanol year with a combined production of about 3.2 Million m3 vinasse or stillage per year. At the moment there is a new 1,000 m3 digester under construction under our supervision at Distillery Fronterita. The country legislation aiming at 5% ethanol in gasoline cannot be reached without wastewater treatment plants, so the industry will have to invest heavily during this and the next two years to be able to reach the goals. The combined biogas production could reach nearly 10,000 cubic meters hourly for 250 days in the year,. Using the simple Frichs plants the combined production of electricity could be in the order of 20 to 30 Mwe and about 60 Mwe heat. The combination with water recycling now being enforced in all industries in Tucuman plus better efficiency in production of ethanol will mean that, with bagasse from sugar cane, the production of electricity could be maintained all year long. Bioelectricity has a premium market value and the whole scheme will be highly economic and environmental friendly. doi:10.1016/j.jbiotec.2010.08.384
Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576
tion enzyme, microbial cells and carbohydrate from outside. The major microbial cells in WBFB were isolated and identified. The variations in compositions of WBFB, ethanol, acetaldehyde and acetic acid including CFU values with stock time were investigated. The effects of process conditions, temperature, working volume and reaction mode e.g. static, shaking and agitation on the production of bio-ethanol were studied. The higher working volume of the medium in a given reactor caused an evident positive effect on the bio-ethanol production. The increase in process temperature in agitation conditions shortened the time required for bio-ethanol production. From this whole study it was found that the use of WBFB is a cheap and suitable source for ethanol production. doi:10.1016/j.jbiotec.2010.08.382 [P-B.30] Ethanol production from sweet sorghum juice in repeatedbatch fermentation by Saccharomyces cerevisiae immobilized on corncob Pattana Laopaiboon ∗ , Lakkana Laopaiboon Khon Kaen University, Thailand Keywords: Corncob; Immobilized yeasts; Repeated batch ethanol fermentation; Sweet sorghum juice; Saccharomyces cerevisiae Sweet sorghum has been promised as a large scale energy crop because its stalks contain high fermentable sugar and it can be cultivated in nearly all temperatures and tropical climate areas Rajvanshi and Nimbkar, 2005. In this study, batch and repeated batch ethanol fermentation from sweet sorghum juice containing total soluble solids of 24 o Brix by Saccharomyces cerevisiae TISTR 5048 and S. cerevisiae NP 01 immobilized on a low cost support material, corncob, were investigated. S. cerevisiae TISTR 5048 immobilized on 6 × 6 × 6-mm3 corncob gave higher ethanol production than those on 12 × 12 × 12-mm3 corncob in terms of ethanol concentration (P), yield (Yp/s ) and productivity (Qp ) with the values of 100.69 g l−1 , 0.45 and 2.10 g l−1 h−1 , respectively. In repeated batch ethanol fermentation by the yeasts immobilized on the 6 × 6 × 6-mm3 corncob could be used at least eight successive cycles without a significant decrease in ethanol production. The average P, Yp/s and Qp of the repeated-batch fermentation in the eight cycles were 93.69 ± 5.03 g l−1 , 0.45 ± 0.01 and 1.95 ± 0.10 g l−1 h−1 , respectively at the fermentation time (each batch) of 48 h. The repeated batch ethanol fermentation by S. cerevisiae NP 01 immobilized on the 6 × 6 × 6-mm3 corncob was also carried out for eight cycles. T-test method showed that P, Yp/s and Qp of the two yeast strains were not significantly different (P > 0.05). The ethanol production efficiencies in the repeated batch fermentation by S. cerevisiae TISTR 5048 immobilized on the 6 × 6 × 6-mm3 corncob were compared with those of the yeast immobilized on 3-mm calcium alginate bead (a widely used support material) Laopaiboon and Laopaiboon, 2006. The results showed that the yeast immobilized on the corncob gave significantly higher P, Yp/s and Qp than those immobilized on calcium alginate (P < 0.05). The results obtained from this study indicate that corncob is a suitable support material to immobilize the yeasts for ethanol production.
References Rajvanshi, A.K. and Nimbkar, N. (2005) Sweet sorghum R&D at the Nimbkar Agricultural Research Institute (NARI). http://nariphaltan.virtualave.net/sorghum.htm. Cited 5 Oct 2005.
Laopaiboon, L., Laopaiboon, P., 2006. Repeated-batch ethanol production from sweet sorghum juice by immobilized yeasts. In: The 8th Agro-Industrial Conference: Food Innovation, June 15-16, 2006, Bangkok, Thailand.
doi:10.1016/j.jbiotec.2010.08.383 [P-B.31] Electricity from biogas. Integrated plan for Tucuman in north west Argentina F. Sineriz 1,2 1
PROIMI Biotech-CONICET, Argentina Microbiology, Fac de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Argentina Keywords: Biofuels; Anaerobic treatment; Citrus processing plants; Distillery waste 2
The North West province of Tucumán, Argentina, is the first world exporter of fresh lemon fruits and one important producer of products of the lemon industrialization. Altogether there are about 35,000 ha dedicated to the production, which is handled by seven processing plants. Under new legislation wastewater treatment is mandatory. Most of the processing plants have chosen anaerobic systems. Actually, there is already one anaerobic system in Citrusvil (one of the two largest lemon processing plants) producing 2900 Nm3 /hour. Another plant for Citricola San Miguel is almost finished and due to start operating during the year. Two another smaller processing plants are currently building 1,000 m3 digesters under our supervision. The combined biogas production arising from anaerobic waste water treatment of the processing plants could reach a total of about 10,000 cubic meters of gas daily. Tucuman also concentrates half of the country production of bioethanol, coming from about 150.000 ha of sugar cane, elaborated in 15 sugar mills. On the distillery front, the production is close to 200,000 m3 ethanol year with a combined production of about 3.2 Million m3 vinasse or stillage per year. At the moment there is a new 1,000 m3 digester under construction under our supervision at Distillery Fronterita. The country legislation aiming at 5% ethanol in gasoline cannot be reached without wastewater treatment plants, so the industry will have to invest heavily during this and the next two years to be able to reach the goals. The combined biogas production could reach nearly 10,000 cubic meters hourly for 250 days in the year,. Using the simple Frichs plants the combined production of electricity could be in the order of 20 to 30 Mwe and about 60 Mwe heat. The combination with water recycling now being enforced in all industries in Tucuman plus better efficiency in production of ethanol will mean that, with bagasse from sugar cane, the production of electricity could be maintained all year long. Bioelectricity has a premium market value and the whole scheme will be highly economic and environmental friendly. doi:10.1016/j.jbiotec.2010.08.384