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ELEVATED ENDOTHELIAL CELL, PLATELET AND T-CELL MICROPARTICLES IN PSORIASIS MAY PROVIDE NOVEL LINK TO ATHEROSCLEROSIS
E2055 JACC March 27, 2012 Volume 59, Issue 13
Vascular Disease ELEVATED ENDOTHELIAL CELL, PLATELET AND T-CELL MICROPARTICLES IN PSORIASIS MAY PROVIDE NOVEL LINK TO ATHEROSCLEROSIS ACC Moderated Poster Contributions McCormick Place South, Hall A Saturday, March 24, 2012, 9:30 a.m.-10:30 a.m.
Session Title: Atherosclerosis and Angiogenesis: Basic and Translational Insights Abstract Category: 34. Vascular - Pathophysiology - Clinical Presentation Number: 1117-125 Authors: Nehal N. Mehta, Ron C. Li, Junko Takeshita, Lifeng Zhang, Abby S. VanVoorhees, Wade Rogers, Mackenzie Wilcox, Anna Raper, Jonni Moore, Joel M. Gelfand, Emile R. Mohler, III, University of Pennsylvania, Cardiovascular Institute, Philadelphia, PA, USA, University of Pennsylvania, Department of Dermatology, Philadelphia, PA, USA Background: Severe psoriasis is a risk factor for cardiovascular (CV) disease beyond traditional risk factors; however, the mechanism remains unknown. Recent findings suggest that cell membrane vesicles, or microparticles, which are released upon cell activation or apoptosis, are associated with CV disease and may play a pathogenic role. Levels of microparticles, particularly from endothelial cells, platelets and T-cells are elevated in patients with cardiovascular disorders and have recently been shown to be predictive of CV outcomes. Methods: To understand if microparticles may serve as a potential link between psoriasis and CV disease, absolute circulating microparticle levels, concentrations, and types (endothelial, platelet, and T cell-derived) of microparticles were measured in blood samples from psoriasis patients (n=20) and compared to healthy controls (n=41). Platelet-poor plasma was separated from whole blood after high-speed centrifugation for microparticle analysis. Microparticles were fluorescently labeled and characterized by flow cytometry. Results: We found higher absolute microparticle levels (2.6-fold increase; 721,123 vs 277,108; p<0.01) and microparticle concentration (5-fold increase; 1,669,625 vs 333,339 particles per uL; p=0.01) in psoriasis compared to healthy controls, which persisted after adjustment for traditional cardiovascular disease risk factors. Characterization of the microparticles revealed a predominance of endothelial-, platelet-, and T cell-derived microparticles, all of which were present in higher absolute levels in psoriasis patients compared to healthy controls (108 vs 37, p<0.01; 70,585 vs 4,727, p<0.01; 995 vs 161, p=0.03, respectively) beyond traditional risk factors (p<0.001 for all). Conclusions: These findings of increased microparticle levels, independent of cardiovascular risk factors, in psoriasis suggests that the presence of increased endothelial cell, platelet and T-cell activation with cell turnover may contribute to the heightened atherosclerosis observed in psoriasis.
Vascular Disease ELEVATED ENDOTHELIAL CELL, PLATELET AND T-CELL MICROPARTICLES IN PSORIASIS MAY PROVIDE NOVEL LINK TO ATHEROSCLEROSIS ACC Moderated Poster Contributions McCormick Place South, Hall A Saturday, March 24, 2012, 9:30 a.m.-10:30 a.m.
Session Title: Atherosclerosis and Angiogenesis: Basic and Translational Insights Abstract Category: 34. Vascular - Pathophysiology - Clinical Presentation Number: 1117-125 Authors: Nehal N. Mehta, Ron C. Li, Junko Takeshita, Lifeng Zhang, Abby S. VanVoorhees, Wade Rogers, Mackenzie Wilcox, Anna Raper, Jonni Moore, Joel M. Gelfand, Emile R. Mohler, III, University of Pennsylvania, Cardiovascular Institute, Philadelphia, PA, USA, University of Pennsylvania, Department of Dermatology, Philadelphia, PA, USA Background: Severe psoriasis is a risk factor for cardiovascular (CV) disease beyond traditional risk factors; however, the mechanism remains unknown. Recent findings suggest that cell membrane vesicles, or microparticles, which are released upon cell activation or apoptosis, are associated with CV disease and may play a pathogenic role. Levels of microparticles, particularly from endothelial cells, platelets and T-cells are elevated in patients with cardiovascular disorders and have recently been shown to be predictive of CV outcomes. Methods: To understand if microparticles may serve as a potential link between psoriasis and CV disease, absolute circulating microparticle levels, concentrations, and types (endothelial, platelet, and T cell-derived) of microparticles were measured in blood samples from psoriasis patients (n=20) and compared to healthy controls (n=41). Platelet-poor plasma was separated from whole blood after high-speed centrifugation for microparticle analysis. Microparticles were fluorescently labeled and characterized by flow cytometry. Results: We found higher absolute microparticle levels (2.6-fold increase; 721,123 vs 277,108; p<0.01) and microparticle concentration (5-fold increase; 1,669,625 vs 333,339 particles per uL; p=0.01) in psoriasis compared to healthy controls, which persisted after adjustment for traditional cardiovascular disease risk factors. Characterization of the microparticles revealed a predominance of endothelial-, platelet-, and T cell-derived microparticles, all of which were present in higher absolute levels in psoriasis patients compared to healthy controls (108 vs 37, p<0.01; 70,585 vs 4,727, p<0.01; 995 vs 161, p=0.03, respectively) beyond traditional risk factors (p<0.001 for all). Conclusions: These findings of increased microparticle levels, independent of cardiovascular risk factors, in psoriasis suggests that the presence of increased endothelial cell, platelet and T-cell activation with cell turnover may contribute to the heightened atherosclerosis observed in psoriasis.