- Email: [email protected]
Elevated level of circulating platelet-derived microparticles in patients with oral squamous cell carcinoma: a possible association with increased procoagulant activity
Oral Presentation Methods: Consecutive 1845 patients (2524 joints) diagnosed as internal derangement (ID) of TMJ were collected from April 2003 to March 2010 in our department. All the patients were examined by MRI and treated by arthroscopy or open surgeries. The findings of interpreting MRI were recorded as positive, suspicious and negative according to the MRI radiographic criteria. After comparing the findings of MRI with those of surgeries, the numbers of true positive, true negative, false positive and false negative were obtained. Through SPSS v.16.0, receiver operator characteristic curve (ROC curve) was made with 1-specificity as abscissa and the sensitivity as ordinate, and the area under the ROC curve was calculated. According to the area, the diagnostic value of MRI was evaluated. Findings and conclusions: Arthroscopic or open surgeries findings confirmed that 207 joints had disc perforation among all joints. MRI findings showed 189 joints were positive, 197 joints suspicious and 2138 joints negative. The true positive accuracy of MRI findings was 102/189 while true negative accuracy was 2075/2138. 42 of the 197 suspicious joints had perforation. The area under the ROC curve was 0.808 (0.77, 0.85), P < 0.05. We concluded that MRI proved to be a good modality to diagnose disc perforation of TMJ, and the diagnostic result of disc perforation by MRI had certain guiding significance in our clinical work.
e175
Fig. 1. Flow cytometry analysis of circulating microparticles (MPs) in platelet-free plasma from healthy subjects and patients with oral squamous cell carcinoma (OSCC). (a) Representative graphs of circulating MPs (blue window) and fluorescent beads for counting (red window). (b) Plasma level of circulating MPs was significantly higher in patients with OSCC compared to healthy subjects. In addition, plasma level of circulating MPs in patients with stages III–IV OSCC was significantly higher than those with stages I–II OSCC. Data were expressed as means ± SD. **P < 0.01; ***P < 0.001.
http://dx.doi.org/10.1016/j.ijom.2015.08.898 Elevated level of circulating platelet-derived microparticles in patients with oral squamous cell carcinoma: a possible association with increased procoagulant activity W. Zhang ∗ , J. Ren, Q. Men, Y. Zhao School and Hospital of Stomatology, Wuhan University, Wuhan, China Background: The association between circulating microparticles (MPs) and oral squamous cell carcinoma (OSCC) remains largely unknown. Objectives: To evaluate the level of circulating platelet-derived MPs (PMPs) in patients with OSCC, and explore its possible association with procoagulant activity. Methods: The circulating PMPs in 63 OSCC patients and 31 healthy volunteers were characterized and quantified by flow cytometric analysis. The coagulation function of patients with OSCC was correspondingly evaluated. The inflammation-related cytokines were detected in plasma by ELISA and in tumor tissues by immunohistochemistry. Findings and conclusion: The levels of total circulating MPs and PMPs were significantly increased in patients with OSCC compared to healthy subjects. Additionally, the data showed the levels of total circulating MPs and PMPs in patients with stages III–IV OSCC were significantly higher than those with stages I–II OSCC (Figs. 1 and 2). Moreover, increased levels of circulating MPs were correlated with the blood hypercoagulation in patients with OSCC (Fig. 3). Most importantly, the levels of IL-6 and TNF␣ in both plasma and tumor tissues were significantly increased in OSCC patients, which were closely correlated with the elevated level of circulating PMPs (Figs. 4–6). More interestingly, we found that the plasma levels of IL-6 and TNF-␣ were positively correlated with the blood hypercoagulation, but there was no statistical significance (Fig. 7). Our studies indicated that the elevated levels
Fig. 2. Flow cytometry analysis of platelet-derived microparticles (PMPs) in platelet-free plasma from healthy subjects and patients with oral squamous cell carcinoma (OSCC). (a) The PMPs was defined as CD31+ CD41+ events in the Q2 window. (b) Plasma level of PMPs was significantly higher in patients with OSCC compared to healthy subjects. In addition, plasma level of PMPs in patients with stages III–IV OSCC was significantly higher than those with stages I–II OSCC. Data were expressed as means ± SD. **P < 0.01; ***P < 0.001.
Fig. 3. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with coagulation function in the patients with oral squamous cell carcinoma (OSCC). (a) The level of FIB was significantly elevated in patients with OSCC compared to healthy subjects. (b, c) There is no significant difference in PT and APTT between healthy subjects and patients with OSCC. (d, g) Plasma levels of circulating MPs and PMPs were significantly positively correlated with the FIB in OSCC. (e–i) Plasma levels of circulating MPs and PMPs were negatively correlated with the APTT and PT in OSCC, but there is no statistical significance. Data were expressed as means ± SD. *P < 0.05.
e176
Oral Presentation
Fig. 4. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with plasma levels of inflammation-related cytokines in the patients with oral squamous cell carcinoma (OSCC). (a, d) The ELISA results showed that the IL-6 and TNF-␣ in plasma were significantly increased in the patients with OSCC compared to those in healthy subjects. (b, c, e, f) The correlation analysis revealed that the plasma levels of IL-6 and TNF-␣ were positively correlated with the levels of circulating MPs and PMPs in OSCC. Data were expressed as means ± SD. *P < 0.05; **P < 0.01.
Fig. 6. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with the expression of TNF-␣ in the patients with oral squamous cell carcinoma (OSCC). (a, b) The immunohistochemistry results showed that the expression of TNF-␣ was significantly increased in the patients with OSCC compared to those in healthy subjects. (c, d) The correlation analysis revealed that the levels of circulating MPs and PMPs were positively correlated with the expression of TNF-␣ in OSCC. Data were expressed as means ± SD. ***P < 0.001.
Fig. 7. The correlation between the blood hypercoagulation with plasma levels of inflammation-related cytokines in the patients with oral squamous cell carcinoma (OSCC). The plasma levels of IL-6 and TNF-␣ were positively correlated with FIB (a, d), and negatively correlated with PT and APTT (b, c, e, f), but there were no statistical significance.
of circulating MPs showed close correlation with the secretion of inflammation-related cytokines and might be a possible cause of the increased procoagulant activity in patients with OSCC. http://dx.doi.org/10.1016/j.ijom.2015.08.899
Fig. 5. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with the expression of IL-6 in the patients with oral squamous cell carcinoma (OSCC). (a, b) The immunohistochemistry results showed that the expression of IL-6 was significantly increased in the patients with OSCC compared to those in healthy subjects. (c, d) The correlation analysis revealed that the levels of circulating MPs and PMPs were positively correlated with the expression of IL-6 in OSCC. Data were expressed as means ± SD. ***P < 0.001.
e175
Fig. 1. Flow cytometry analysis of circulating microparticles (MPs) in platelet-free plasma from healthy subjects and patients with oral squamous cell carcinoma (OSCC). (a) Representative graphs of circulating MPs (blue window) and fluorescent beads for counting (red window). (b) Plasma level of circulating MPs was significantly higher in patients with OSCC compared to healthy subjects. In addition, plasma level of circulating MPs in patients with stages III–IV OSCC was significantly higher than those with stages I–II OSCC. Data were expressed as means ± SD. **P < 0.01; ***P < 0.001.
http://dx.doi.org/10.1016/j.ijom.2015.08.898 Elevated level of circulating platelet-derived microparticles in patients with oral squamous cell carcinoma: a possible association with increased procoagulant activity W. Zhang ∗ , J. Ren, Q. Men, Y. Zhao School and Hospital of Stomatology, Wuhan University, Wuhan, China Background: The association between circulating microparticles (MPs) and oral squamous cell carcinoma (OSCC) remains largely unknown. Objectives: To evaluate the level of circulating platelet-derived MPs (PMPs) in patients with OSCC, and explore its possible association with procoagulant activity. Methods: The circulating PMPs in 63 OSCC patients and 31 healthy volunteers were characterized and quantified by flow cytometric analysis. The coagulation function of patients with OSCC was correspondingly evaluated. The inflammation-related cytokines were detected in plasma by ELISA and in tumor tissues by immunohistochemistry. Findings and conclusion: The levels of total circulating MPs and PMPs were significantly increased in patients with OSCC compared to healthy subjects. Additionally, the data showed the levels of total circulating MPs and PMPs in patients with stages III–IV OSCC were significantly higher than those with stages I–II OSCC (Figs. 1 and 2). Moreover, increased levels of circulating MPs were correlated with the blood hypercoagulation in patients with OSCC (Fig. 3). Most importantly, the levels of IL-6 and TNF␣ in both plasma and tumor tissues were significantly increased in OSCC patients, which were closely correlated with the elevated level of circulating PMPs (Figs. 4–6). More interestingly, we found that the plasma levels of IL-6 and TNF-␣ were positively correlated with the blood hypercoagulation, but there was no statistical significance (Fig. 7). Our studies indicated that the elevated levels
Fig. 2. Flow cytometry analysis of platelet-derived microparticles (PMPs) in platelet-free plasma from healthy subjects and patients with oral squamous cell carcinoma (OSCC). (a) The PMPs was defined as CD31+ CD41+ events in the Q2 window. (b) Plasma level of PMPs was significantly higher in patients with OSCC compared to healthy subjects. In addition, plasma level of PMPs in patients with stages III–IV OSCC was significantly higher than those with stages I–II OSCC. Data were expressed as means ± SD. **P < 0.01; ***P < 0.001.
Fig. 3. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with coagulation function in the patients with oral squamous cell carcinoma (OSCC). (a) The level of FIB was significantly elevated in patients with OSCC compared to healthy subjects. (b, c) There is no significant difference in PT and APTT between healthy subjects and patients with OSCC. (d, g) Plasma levels of circulating MPs and PMPs were significantly positively correlated with the FIB in OSCC. (e–i) Plasma levels of circulating MPs and PMPs were negatively correlated with the APTT and PT in OSCC, but there is no statistical significance. Data were expressed as means ± SD. *P < 0.05.
e176
Oral Presentation
Fig. 4. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with plasma levels of inflammation-related cytokines in the patients with oral squamous cell carcinoma (OSCC). (a, d) The ELISA results showed that the IL-6 and TNF-␣ in plasma were significantly increased in the patients with OSCC compared to those in healthy subjects. (b, c, e, f) The correlation analysis revealed that the plasma levels of IL-6 and TNF-␣ were positively correlated with the levels of circulating MPs and PMPs in OSCC. Data were expressed as means ± SD. *P < 0.05; **P < 0.01.
Fig. 6. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with the expression of TNF-␣ in the patients with oral squamous cell carcinoma (OSCC). (a, b) The immunohistochemistry results showed that the expression of TNF-␣ was significantly increased in the patients with OSCC compared to those in healthy subjects. (c, d) The correlation analysis revealed that the levels of circulating MPs and PMPs were positively correlated with the expression of TNF-␣ in OSCC. Data were expressed as means ± SD. ***P < 0.001.
Fig. 7. The correlation between the blood hypercoagulation with plasma levels of inflammation-related cytokines in the patients with oral squamous cell carcinoma (OSCC). The plasma levels of IL-6 and TNF-␣ were positively correlated with FIB (a, d), and negatively correlated with PT and APTT (b, c, e, f), but there were no statistical significance.
of circulating MPs showed close correlation with the secretion of inflammation-related cytokines and might be a possible cause of the increased procoagulant activity in patients with OSCC. http://dx.doi.org/10.1016/j.ijom.2015.08.899
Fig. 5. The correlation between circulating microparticles (MPs) or plateletderived microparticles (PMPs) with the expression of IL-6 in the patients with oral squamous cell carcinoma (OSCC). (a, b) The immunohistochemistry results showed that the expression of IL-6 was significantly increased in the patients with OSCC compared to those in healthy subjects. (c, d) The correlation analysis revealed that the levels of circulating MPs and PMPs were positively correlated with the expression of IL-6 in OSCC. Data were expressed as means ± SD. ***P < 0.001.