- Email: [email protected]
ElncRNA1, a Long Noncoding RNA that is Transcriptionally Induced by Oestrogen, Promotes Epithelial Ovarian Cancer Cell Proliferation
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Abstracts / Journal of Minimally Invasive Gynecology 24 (2017) S1–S201
Virtual Poster Sessions TUESDAY, NOVEMBER 14, 2017 311
Virtual Posters – Session 1 (9:45 AM - 10:45 AM) 9:45 AM – STATION A
An Assessment of the Global Health Interest in the Minimally Invasive Gynecologic Surgery Community and Perceived Barriers Jan AG, Ito T, Gaskins J, Pasic R, Biscette S. University of Louisville, Louisville, Kentucky Study Objective: To assess the global health interest within the minimally invasive gynecologic surgery community, and to identify perceived barriers and motivations. Design: A modified survey approved by the institutional board review was distributed to AAGL members via email. Responses were collected over a 2-month period. Summary statistics and graphical depictions were utilized to describe the responses from the survey data. Chi-squared tests were used to test for differences in key outcomes in demographic predictors. Multivariate logistic regression was used to control for confounding variables. A significance level of alpha 0.05 was used throughout. Setting: Approximately 2 billion people have no access to basic surgical care and 17–18 million people die from conditions preventable by surgery. When appropriate, minimally invasive surgery would be the ideal method for underserved populations with limited access to care, requiring less recovery time and less complications. Measurements and Main Results: 209 surgeons completed the survey. 51% of the participants responded they were extremely likely or very likely to participate in a medical mission abroad. Participants were motivated by fulfilling the true medical creed and personal altruistic goals, (70%, 56%, respectively). All but two motivators, (developing international contacts/ networking, P = .1834; and religious obligations/aspirations, P = .4177), reached statistical significance in univariate analysis. The most common barriers were scheduling conflicts, and financial costs, (60%, 55%, respectively). Two out of ten perceived barriers reached statistical significance in univariate analysis, (family/social responsibilities, P = .0002; and personal safety, P = .0455). Conclusion: Minimally invasive gynecologic surgeons are interested in participating in medical missions globally. Organizing fundraisers to decrease overall costs, allowing designated time for global health endeavors, and establishing a database through larger organizations such as the AAGL could be helpful in the future to promote surgeon efforts abroad. 312
Virtual Posters – Session 1 (9:45 AM - 10:45 AM) 9:45 AM – STATION B
ElncRNA1, a Long Noncoding RNA that is Transcriptionally Induced by Oestrogen, Promotes Epithelial Ovarian Cancer Cell Proliferation Qiu J, Hua K-Q. Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China Study Objective: We previously identified a novel oestrogen (E2)upregulated lncRNA, TC0101441, via microarray analysis. However, the detailed mechanism by which E2 upregulates TC0101441 and the role of TC0101441 in epithelial ovarian cancer (EOC) progression have not been elucidated. In the present study, we further analysed TC0101441, which we designated oestrogen-induced long non-coding RNA-1 (ElncRNA1), and investigate the function and underlying mechanisms of ElncRNA1 in E2dependant EOC progression.
Design: Retrospective study, in vitro and in vivo study. Setting: Obstetrics and Gynecology Hospital of Fudan University. Patients: EOC patients admitted to the Obstetrics and Gynecology Hospital of Fudan University between 2014 and 2016 Intervention: A serial of assays were performed to determine the mechanism by which E2 upregulates ElncRNA1. Clinically, ElncRNA1 expression in EOC tissues was examined. In vitro and in vivo functional assays were performed to elucidate the role of ElncRNA1 in E2-dependant EOC progression. Measurements and Main Results: We showed that E2 transcriptionally upregulates ElncRNA1 through the oestrogen receptor α (ERα)-oestrogen response element (ERE) pathway using RNA stability assays, bioinformaticsbased searches for ERE binding sites, chromatin immunoprecipitation (ChIP) assays and dual luciferase reporter assays. Clinically, ElncRNA1 levels are significantly higher in EOC tissues than in normal ovarian surface epithelium. In vitro and in vivo loss-of-function assays revealed that ElncRNA1 promotes EOC cell proliferation. This pro-proliferation effect of ElncRNA1 was partially mediated by the regulation of Cyclin D1/CDK4/CDK6 pathway. Conclusion: These findings provide the first evidence that E2 upregulates ElncRNA1 at the transcriptional level through the ERα-ERE pathway and that this novel E2-upregulated lncRNA has an oncogenic role in EOC growth. The placement of ElncRNA1 in the E2-ERα-ERE-Cyclin D1/CDK4/ CDK6 signalling pathway may provide greater insight into the effects of oestrogen on EOC progression from the perspective of lncRNA.
313
Virtual Posters – Session 1 (9:45 AM - 10:45 AM) 9:45 AM – STATION C
Evaluating Research Pipelines in Clinical Research for Minimally Invasive Gynecologic Surgery Guidelines Wright MR,1 Vassar M,2 Gordon J,2 Frye L,1 Po W,1 Babb C1. 1OSU Ob/Gyn Department, Oklahoma State University Medical Center, Tulsa, Oklahoma; 2Clinical Research-Psychiatry & Behavioral Sciences, Oklahoma State University Center for Health Sciences, Tulsa, Oklahoma Study Objective: To explore the integrity of the research pipeline in minimally invasive gynecologic surgery by evaluating the extent to which research gaps, identified from low and very low quality evidence (Level C) during CPG development, are being addressed by new and ongoing research catalogued in clinical trials registries. The secondary outcome is to determine if there are possible misappropriations of funding for ongoing clinical research for which there is already sufficient evidence (Level A). Design: Cross-sectional study. Setting: Academic hospital and associated center for health sciences. Measurements and Main Results: We located guidelines from the AAGL’s website. For each recommendation based on low or very low quality evidence, a research question using the PICO framework (Population, Intervention, Comparison, and Outcome) was written to assist in searching trial registries. Key words from each PICO question were used to develop search queries for the trial registries. After the search queries were finalized, a search of both ClinicalTrials.gov and the World Health Organization’s International Clinical Trials Registry Platform was performed to locate new and ongoing studies. These studies were then screened for sensitivity and specificity based on each PICO question. Recommendations were organized based on level of evidence (A&C) and the number of new or ongoing clinical trials for each recommendation. Conclusion: Results from this study can be used to inform research priority setting by showcasing areas of greatest need. Furthermore, areas where excess research is being conducted will be highlighted, and implications for research funding will be discussed.
Abstracts / Journal of Minimally Invasive Gynecology 24 (2017) S1–S201
Virtual Poster Sessions TUESDAY, NOVEMBER 14, 2017 311
Virtual Posters – Session 1 (9:45 AM - 10:45 AM) 9:45 AM – STATION A
An Assessment of the Global Health Interest in the Minimally Invasive Gynecologic Surgery Community and Perceived Barriers Jan AG, Ito T, Gaskins J, Pasic R, Biscette S. University of Louisville, Louisville, Kentucky Study Objective: To assess the global health interest within the minimally invasive gynecologic surgery community, and to identify perceived barriers and motivations. Design: A modified survey approved by the institutional board review was distributed to AAGL members via email. Responses were collected over a 2-month period. Summary statistics and graphical depictions were utilized to describe the responses from the survey data. Chi-squared tests were used to test for differences in key outcomes in demographic predictors. Multivariate logistic regression was used to control for confounding variables. A significance level of alpha 0.05 was used throughout. Setting: Approximately 2 billion people have no access to basic surgical care and 17–18 million people die from conditions preventable by surgery. When appropriate, minimally invasive surgery would be the ideal method for underserved populations with limited access to care, requiring less recovery time and less complications. Measurements and Main Results: 209 surgeons completed the survey. 51% of the participants responded they were extremely likely or very likely to participate in a medical mission abroad. Participants were motivated by fulfilling the true medical creed and personal altruistic goals, (70%, 56%, respectively). All but two motivators, (developing international contacts/ networking, P = .1834; and religious obligations/aspirations, P = .4177), reached statistical significance in univariate analysis. The most common barriers were scheduling conflicts, and financial costs, (60%, 55%, respectively). Two out of ten perceived barriers reached statistical significance in univariate analysis, (family/social responsibilities, P = .0002; and personal safety, P = .0455). Conclusion: Minimally invasive gynecologic surgeons are interested in participating in medical missions globally. Organizing fundraisers to decrease overall costs, allowing designated time for global health endeavors, and establishing a database through larger organizations such as the AAGL could be helpful in the future to promote surgeon efforts abroad. 312
Virtual Posters – Session 1 (9:45 AM - 10:45 AM) 9:45 AM – STATION B
ElncRNA1, a Long Noncoding RNA that is Transcriptionally Induced by Oestrogen, Promotes Epithelial Ovarian Cancer Cell Proliferation Qiu J, Hua K-Q. Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China Study Objective: We previously identified a novel oestrogen (E2)upregulated lncRNA, TC0101441, via microarray analysis. However, the detailed mechanism by which E2 upregulates TC0101441 and the role of TC0101441 in epithelial ovarian cancer (EOC) progression have not been elucidated. In the present study, we further analysed TC0101441, which we designated oestrogen-induced long non-coding RNA-1 (ElncRNA1), and investigate the function and underlying mechanisms of ElncRNA1 in E2dependant EOC progression.
Design: Retrospective study, in vitro and in vivo study. Setting: Obstetrics and Gynecology Hospital of Fudan University. Patients: EOC patients admitted to the Obstetrics and Gynecology Hospital of Fudan University between 2014 and 2016 Intervention: A serial of assays were performed to determine the mechanism by which E2 upregulates ElncRNA1. Clinically, ElncRNA1 expression in EOC tissues was examined. In vitro and in vivo functional assays were performed to elucidate the role of ElncRNA1 in E2-dependant EOC progression. Measurements and Main Results: We showed that E2 transcriptionally upregulates ElncRNA1 through the oestrogen receptor α (ERα)-oestrogen response element (ERE) pathway using RNA stability assays, bioinformaticsbased searches for ERE binding sites, chromatin immunoprecipitation (ChIP) assays and dual luciferase reporter assays. Clinically, ElncRNA1 levels are significantly higher in EOC tissues than in normal ovarian surface epithelium. In vitro and in vivo loss-of-function assays revealed that ElncRNA1 promotes EOC cell proliferation. This pro-proliferation effect of ElncRNA1 was partially mediated by the regulation of Cyclin D1/CDK4/CDK6 pathway. Conclusion: These findings provide the first evidence that E2 upregulates ElncRNA1 at the transcriptional level through the ERα-ERE pathway and that this novel E2-upregulated lncRNA has an oncogenic role in EOC growth. The placement of ElncRNA1 in the E2-ERα-ERE-Cyclin D1/CDK4/ CDK6 signalling pathway may provide greater insight into the effects of oestrogen on EOC progression from the perspective of lncRNA.
313
Virtual Posters – Session 1 (9:45 AM - 10:45 AM) 9:45 AM – STATION C
Evaluating Research Pipelines in Clinical Research for Minimally Invasive Gynecologic Surgery Guidelines Wright MR,1 Vassar M,2 Gordon J,2 Frye L,1 Po W,1 Babb C1. 1OSU Ob/Gyn Department, Oklahoma State University Medical Center, Tulsa, Oklahoma; 2Clinical Research-Psychiatry & Behavioral Sciences, Oklahoma State University Center for Health Sciences, Tulsa, Oklahoma Study Objective: To explore the integrity of the research pipeline in minimally invasive gynecologic surgery by evaluating the extent to which research gaps, identified from low and very low quality evidence (Level C) during CPG development, are being addressed by new and ongoing research catalogued in clinical trials registries. The secondary outcome is to determine if there are possible misappropriations of funding for ongoing clinical research for which there is already sufficient evidence (Level A). Design: Cross-sectional study. Setting: Academic hospital and associated center for health sciences. Measurements and Main Results: We located guidelines from the AAGL’s website. For each recommendation based on low or very low quality evidence, a research question using the PICO framework (Population, Intervention, Comparison, and Outcome) was written to assist in searching trial registries. Key words from each PICO question were used to develop search queries for the trial registries. After the search queries were finalized, a search of both ClinicalTrials.gov and the World Health Organization’s International Clinical Trials Registry Platform was performed to locate new and ongoing studies. These studies were then screened for sensitivity and specificity based on each PICO question. Recommendations were organized based on level of evidence (A&C) and the number of new or ongoing clinical trials for each recommendation. Conclusion: Results from this study can be used to inform research priority setting by showcasing areas of greatest need. Furthermore, areas where excess research is being conducted will be highlighted, and implications for research funding will be discussed.