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Elongated silica microparticles for enhanced delivery of tailorable nanoemulsion as a potential platform for transdermal drug delivery
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Abstracts from the 41st Annual Meeting / Journal of Dermatological Science 86 (2017) e1–e95
P04-12[C06-8]
P04-13[C06-5]
Elongated silica microparticles for enhanced delivery of tailorable nanoemulsion as a potential platform for transdermal drug delivery
Metabolomic analysis of sweat revealed glucose as a biomarker of atopic dermatitis
Miko Yamada 1,∗ , Hossam Tayeb 2 , Hequn Wang 3 , Nhung Dang 1 , Anthony Raphael 3 , Paul J. Belt 4 , Peter H. Soyer 1 , Conor L. Evans 3 , Frank Sainsbury 2 , Tarl Prow 1 1 University of Queensland, School of Medicine, Dermatology Research Centre, Translational Research Institute, Brisbane, Australia 2 Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Princess Alexandra Hospital, Brisbane, Australia 3 Wellman Centre for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, USA 4 Department of Plastic and Reconstructive Surgery and Orthopaedic Surgery, Princess Alexandra Hospital, Brisbane, Australia The purpose of this research was to evaluate the combination of elongated silica microparticles (EMP) and tailorable nanoemulsions (TNE) to control topical delivery of hydrophobic drug surrogates. The microparticles penetrate through the epidermis and stop at the dermal-epidermal junction (DEJ). TNE is unusually stable because the oil core allows high loading levels and the surface properties can be easily controlled. In this study we incorporated a fluorescent lipophilic dye, DiI, as a hydrophobic drug surrogate into TNE for visualization with microscopy. In addition, the core droplet of TNE was packed with pharmaceutical grade lipid (glycerol) instead of DiI and imaged by coherent anti-Stoke Raman scattering (CARS) microscopy to characterize the delivery of lipid in freshly excised human skin. We compared four different coating approaches to combine EMP and TNE. These data showed that a freeze-dried formulation with alginate cross-linking showed 100% of the detectable TNE were retained on the EMP. When this dry form of EMP-TNE was applied to excised, living human abdominal skin, the EMP penetrated to the DEJ and we observed that the controlled release of TNE thereafter. This formulation resulted in a sustained release profile, whereas a freeze dried formulation without crosslinking showed an immediate burst type of release profile. DiI could be detected as deep as 60 micron into the skin showing a potential usage of TNE as a hydrophobic drug carrier in combination with a physical penetration enhancing technology. These data show that a dry, slow release formulation containing EMP coated with TNE can effectively deliver a hydrophobic payload deep into the human epidermis and controllably release that payload.
http://dx.doi.org/10.1016/j.jdermsci.2017.02.087
Emi Ono 1,∗ , Hiroyuki Murota 1 , Yuki Mori 2 , Yoshichika Yoshioka 2 , Ichiro Katayama 1 1
The Department of Dermatology, University of Osaka, Osaka, Japan 2 Biofunctional Imaging Lab, Immunology Frontier Research Center (IFReC), Osaka University, Osaka, Japan Background: Sweat has been considered as a major aggravating factor of atopic dermatitis (AD). Change in the constituents in sweat of AD has been considered to involve in its pathogenesis. In recent years, metabolites in sweat are receiving increasing attention as a biomarker of certain diseases. Objective: Thus, metabolomic analysis of sweat was performed to explore the metabolites contributed to the etiology of AD by use of nuclear magnetic resonance (NMR). Methods: Healthy (n = 6, age range 28–45, mean 34, all male) and AD subjects (n = 13, age range 17–50, mean 34, male:female = 10:3) were employed to this study. Sweat is collected from the back under the circumstances of sauna bathing. Several properties of sweat (PH, protein concentration, Na+, and salt concentration) were measured. Expression level of glucose transporters was evaluated by immunohistochemistry, and was quantified by real-time PCR using sweat gland collected with laser capture microdissection. Results: NMR spectrum of the back sweat derived from healthy subjects was characterized by intense signals of lactate, pyruvate, and glycerol. Peak in glucose were found specifically in sweat derived from subjects with AD. Measurement of glucose concentration of sweat of AD found the significant correlation between sweat glucose concentration and SCORAD score (p = 0.0003). Furthermore, immunohistochemistry revealed that the glucose transporter, GLUT2, expressed in the human sweat gland. GLUT2 located in cytoplasm of secretory cells with appearance of granule in non-AD skin, while located on the lumen of secretory cells in AD-lesional skin. Conclusion: Glucose secreted via glucose transporter on sweat gland might be a biomarker for severity of AD, and might contribute to the etiology of this disease. http://dx.doi.org/10.1016/j.jdermsci.2017.02.088 P04-14[C11-5] Up-regulation of HHV-6 microRNAs in the serum of DIHS/DRESS patients Kazuya Miyashita ∗ , Fumi Miyagawa, Yuki Nakamura, Rie Onmori, Hiroaki Azukizawa, Hideo Asada Department of Dermatology, Nara Medical University School of Medicine, Kashihara, Japan Drug-induced hypersensitivity syndrome/drug reaction with eosinophilia and systemic symptoms (DIHS/DRESS) is a particular type of severe cutaneous adverse drug reaction associated with the reactivation of human herpesvirus 6 (HHV-6). Recent studies have highlighted the importance of herpesvirus-derived microRNAs (miRNAs) in modulating cellular and viral gene expression. MiRNAs are non-protein-coding small RNA in the size range of 19–25 nucleotides that play important roles in biological processes. Recently four HHV-6-derived miRNAs, hhv6b-miR-Ro6-1, 2, 3 and 4 (HHV6-miR-1, 2, 3 and 4), have been identified. However, the precise role of these miRNAs in regulating HHV-6 latency
Abstracts from the 41st Annual Meeting / Journal of Dermatological Science 86 (2017) e1–e95
P04-12[C06-8]
P04-13[C06-5]
Elongated silica microparticles for enhanced delivery of tailorable nanoemulsion as a potential platform for transdermal drug delivery
Metabolomic analysis of sweat revealed glucose as a biomarker of atopic dermatitis
Miko Yamada 1,∗ , Hossam Tayeb 2 , Hequn Wang 3 , Nhung Dang 1 , Anthony Raphael 3 , Paul J. Belt 4 , Peter H. Soyer 1 , Conor L. Evans 3 , Frank Sainsbury 2 , Tarl Prow 1 1 University of Queensland, School of Medicine, Dermatology Research Centre, Translational Research Institute, Brisbane, Australia 2 Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Princess Alexandra Hospital, Brisbane, Australia 3 Wellman Centre for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, USA 4 Department of Plastic and Reconstructive Surgery and Orthopaedic Surgery, Princess Alexandra Hospital, Brisbane, Australia The purpose of this research was to evaluate the combination of elongated silica microparticles (EMP) and tailorable nanoemulsions (TNE) to control topical delivery of hydrophobic drug surrogates. The microparticles penetrate through the epidermis and stop at the dermal-epidermal junction (DEJ). TNE is unusually stable because the oil core allows high loading levels and the surface properties can be easily controlled. In this study we incorporated a fluorescent lipophilic dye, DiI, as a hydrophobic drug surrogate into TNE for visualization with microscopy. In addition, the core droplet of TNE was packed with pharmaceutical grade lipid (glycerol) instead of DiI and imaged by coherent anti-Stoke Raman scattering (CARS) microscopy to characterize the delivery of lipid in freshly excised human skin. We compared four different coating approaches to combine EMP and TNE. These data showed that a freeze-dried formulation with alginate cross-linking showed 100% of the detectable TNE were retained on the EMP. When this dry form of EMP-TNE was applied to excised, living human abdominal skin, the EMP penetrated to the DEJ and we observed that the controlled release of TNE thereafter. This formulation resulted in a sustained release profile, whereas a freeze dried formulation without crosslinking showed an immediate burst type of release profile. DiI could be detected as deep as 60 micron into the skin showing a potential usage of TNE as a hydrophobic drug carrier in combination with a physical penetration enhancing technology. These data show that a dry, slow release formulation containing EMP coated with TNE can effectively deliver a hydrophobic payload deep into the human epidermis and controllably release that payload.
http://dx.doi.org/10.1016/j.jdermsci.2017.02.087
Emi Ono 1,∗ , Hiroyuki Murota 1 , Yuki Mori 2 , Yoshichika Yoshioka 2 , Ichiro Katayama 1 1
The Department of Dermatology, University of Osaka, Osaka, Japan 2 Biofunctional Imaging Lab, Immunology Frontier Research Center (IFReC), Osaka University, Osaka, Japan Background: Sweat has been considered as a major aggravating factor of atopic dermatitis (AD). Change in the constituents in sweat of AD has been considered to involve in its pathogenesis. In recent years, metabolites in sweat are receiving increasing attention as a biomarker of certain diseases. Objective: Thus, metabolomic analysis of sweat was performed to explore the metabolites contributed to the etiology of AD by use of nuclear magnetic resonance (NMR). Methods: Healthy (n = 6, age range 28–45, mean 34, all male) and AD subjects (n = 13, age range 17–50, mean 34, male:female = 10:3) were employed to this study. Sweat is collected from the back under the circumstances of sauna bathing. Several properties of sweat (PH, protein concentration, Na+, and salt concentration) were measured. Expression level of glucose transporters was evaluated by immunohistochemistry, and was quantified by real-time PCR using sweat gland collected with laser capture microdissection. Results: NMR spectrum of the back sweat derived from healthy subjects was characterized by intense signals of lactate, pyruvate, and glycerol. Peak in glucose were found specifically in sweat derived from subjects with AD. Measurement of glucose concentration of sweat of AD found the significant correlation between sweat glucose concentration and SCORAD score (p = 0.0003). Furthermore, immunohistochemistry revealed that the glucose transporter, GLUT2, expressed in the human sweat gland. GLUT2 located in cytoplasm of secretory cells with appearance of granule in non-AD skin, while located on the lumen of secretory cells in AD-lesional skin. Conclusion: Glucose secreted via glucose transporter on sweat gland might be a biomarker for severity of AD, and might contribute to the etiology of this disease. http://dx.doi.org/10.1016/j.jdermsci.2017.02.088 P04-14[C11-5] Up-regulation of HHV-6 microRNAs in the serum of DIHS/DRESS patients Kazuya Miyashita ∗ , Fumi Miyagawa, Yuki Nakamura, Rie Onmori, Hiroaki Azukizawa, Hideo Asada Department of Dermatology, Nara Medical University School of Medicine, Kashihara, Japan Drug-induced hypersensitivity syndrome/drug reaction with eosinophilia and systemic symptoms (DIHS/DRESS) is a particular type of severe cutaneous adverse drug reaction associated with the reactivation of human herpesvirus 6 (HHV-6). Recent studies have highlighted the importance of herpesvirus-derived microRNAs (miRNAs) in modulating cellular and viral gene expression. MiRNAs are non-protein-coding small RNA in the size range of 19–25 nucleotides that play important roles in biological processes. Recently four HHV-6-derived miRNAs, hhv6b-miR-Ro6-1, 2, 3 and 4 (HHV6-miR-1, 2, 3 and 4), have been identified. However, the precise role of these miRNAs in regulating HHV-6 latency