- Email: [email protected]
Embryonic death due to lead exposure
110
The frequency of structural anomalies in the bone marrow cells was significant after 6, 12 and 24 h with both doses. The highest frequency was observed after 12 h with as well 120 mg as 240 mg NBU. Likewise the l y m p h o c y t e chromosomes showed the highest aberration rate as well after 12 h, here again with both doses. Comparing the results of the bone marrow with those of the lymphocytes, it could be seen that the aberration rate of the bone marrow was remarkably higher than the rate of the aberrant metaphases of the lymphocytes, and that with all intervals and both doses. Our preliminary results of the spermatogonia indicate a high frequency of chromosome aberrations with the doses between 30 mg/kg and 60 mg/kg b o d y weight NBU, whereas the significant toxicity of the high doses of 120 mg and 240 mg made genetic analysis impossible. This w o r k w a s s p o n s o r e d b y the D e u t s c h e F o r s c h u n g s g e m e i n s c h a f t .
17 P. Jacquet, A. Leonard and G.B. Gerber, Dept. of Radiobiology, CEN~SCK, Mol (Belgium) Embryonic death due to lead exposure Female mice with vaginal plug (0.5 day of pregnancy) were given a diet containing 0, 0.125, 0.250 or 0.500% of lead. They were dissected 15 to 18 days later and examined for the presence of live and dead embryos, and for the number of corpora lutea. The weight of the embryos was also recorded. Such treatment was found to reduce significantly the incidence of pregnancies, and, for the pregnant females, to increase the post-implantation loss and to reduce the pre-implantation loss. The growth of the embryos was also reduced. All these effects were noticeable from doses of 0.25% of lead on. In other experiments, we killed female mice 48 h after vaginal plug and treatm e n t with 0.5% of lead in diet. It appeared that this treatment retards the early divisions of the embryos b u t does n o t prevent them altogether.
18 W. Reichert, Institut fiir Anthropologie und Humangenetik, Heidelberg (Federal Republic of Germany) Elimination of X-ray induced chromosome aberrations in embryogenesis of mice NMRI-random bred females were treated with PMS (pregnant mares' serum) and HCG (human chorionic gonadotrophin) for stimulated ovulation. Three hours after the application of HCG, the females were irradiated with 200 R
The frequency of structural anomalies in the bone marrow cells was significant after 6, 12 and 24 h with both doses. The highest frequency was observed after 12 h with as well 120 mg as 240 mg NBU. Likewise the l y m p h o c y t e chromosomes showed the highest aberration rate as well after 12 h, here again with both doses. Comparing the results of the bone marrow with those of the lymphocytes, it could be seen that the aberration rate of the bone marrow was remarkably higher than the rate of the aberrant metaphases of the lymphocytes, and that with all intervals and both doses. Our preliminary results of the spermatogonia indicate a high frequency of chromosome aberrations with the doses between 30 mg/kg and 60 mg/kg b o d y weight NBU, whereas the significant toxicity of the high doses of 120 mg and 240 mg made genetic analysis impossible. This w o r k w a s s p o n s o r e d b y the D e u t s c h e F o r s c h u n g s g e m e i n s c h a f t .
17 P. Jacquet, A. Leonard and G.B. Gerber, Dept. of Radiobiology, CEN~SCK, Mol (Belgium) Embryonic death due to lead exposure Female mice with vaginal plug (0.5 day of pregnancy) were given a diet containing 0, 0.125, 0.250 or 0.500% of lead. They were dissected 15 to 18 days later and examined for the presence of live and dead embryos, and for the number of corpora lutea. The weight of the embryos was also recorded. Such treatment was found to reduce significantly the incidence of pregnancies, and, for the pregnant females, to increase the post-implantation loss and to reduce the pre-implantation loss. The growth of the embryos was also reduced. All these effects were noticeable from doses of 0.25% of lead on. In other experiments, we killed female mice 48 h after vaginal plug and treatm e n t with 0.5% of lead in diet. It appeared that this treatment retards the early divisions of the embryos b u t does n o t prevent them altogether.
18 W. Reichert, Institut fiir Anthropologie und Humangenetik, Heidelberg (Federal Republic of Germany) Elimination of X-ray induced chromosome aberrations in embryogenesis of mice NMRI-random bred females were treated with PMS (pregnant mares' serum) and HCG (human chorionic gonadotrophin) for stimulated ovulation. Three hours after the application of HCG, the females were irradiated with 200 R