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Endometrial antigens eliciting auto-antibody responses in patients with endometriosis
P 292
195
PERITONEAL-FLUID CELL-MEDIATED AND ~UMORAL FACTORS IN INFERTILE WOMEN
L.V.VANKO, M.A.NIKOLAEVA, N.I.VOLKOV, N.S.SULEIMANOVA, T.Ya.PSPENICHNIKOVA and G.T.SUKHIKH, All-Union Research Centre for Maternal and Child Health,
Moscow 117815 USSR There is evidence that soluble products of activated im~nunocompetent cells may interfere with the reproductive process. Therefore their role in endometriosis-associated infertility is explored. Peritoneal fluid cell composition was analysed by direct flow cytofluorometry, using the FacScan device and monoclonal antibodies (Ortho Diagnostic System and Becton Dickinson), in infertile patients (minor external endometriosis as group I and pelvic adhesions as group 2). Cytotoxic effect of peritoneal fluid on donor sperms and target cells L-929, sensitive to tumor-necrotizing factor (TNF), was assessed. Spontaneous LPS- or opioid receptor ligand-induced TNF activity was evaluated in macrophage supernatant cultures. Altered macrophage/Lymphocyte ratio, and significantly increased content of DR+-macrophages were demonsarated in peritoneal fluid of endometriosis patients. Wide individual variability of sperm toxicity ( 20 to 60% ) and TNF activity (21 to 99% at 1:1 dilution) was demonstrated in peritoneal fluid of patients from both groups. There were no significant differences in the respective mean values between the groups. The results are suggestive of increased content-of cells, exoressing secondclass HLA antigens, particularly so in endometriosis patients. Activation of macrophage cells and their cytokine secretion may be the actual basis for gamete- and embryotoxic effects contributing to infertility in patients with endometriosis and pelvic adhesions. Endometriosis,Macrophages,Tumor necrosis factor.
P 293 E N D O M E T R I A L ANTIGENS ELICITING IN PATIENTS WITH ENDOMETRIOSIS.
AUTO-ANTIBODY
RESPONSES
S. MATHUR, D. GARZA, L.F. SMITH and H.O. WILLIAMSON, Dept. of Ob/Gyn, Medical University of South Carolina, Charleston, SC 29425. Endometrial antigens from patients with endometriosis and controls reactive against IgG, IgA or IgM antibodies in serum and peritoneal fluid of 7 endometriosis patients and 8 controls were identified by Western blot analysis. Serum and peritoneal fluid IgG from the patients reacted against endometrial antigens with molecular weight (MW) of 26 kD (present only in the endometrium from patients), 34 kD (present predominently in the patient endometrium) and 42 kD (present only in the patients' endometria). Serum and peritoneal fluid IgG from the controls failed to react against these antigens. Serum and peritoneal fluid IgA of both groups reacted against common endometrial antigens with MW of 25, 52, 58 and 66 kD and antigens with MW of 48, 60,100 and 120 kD detectable only in endometrium of patients. Three patients had serum IgA antibodies against patient endometrial antigen with MW 52 kD. Serum and peritoneal fluid IgM of both patients and controls reacted against common endometrial antigens with MW of 25, 46, 48, 56, 60, 66, 80 and 90 kD and antigen with MW of 52 kD detectable only in patient endometrium. It is concluded that endometrial antibody response belonging to IgG isotype is relevant to endometrial autoimrrunity in patients with endometriosis and that IgG antibodies are directed against antigens with MW 26, 34 and 42 kD found only in patient endometrium. Key words: Autoimmunity,endometriosis,endometrialantigens,endometrialantibodies
195
PERITONEAL-FLUID CELL-MEDIATED AND ~UMORAL FACTORS IN INFERTILE WOMEN
L.V.VANKO, M.A.NIKOLAEVA, N.I.VOLKOV, N.S.SULEIMANOVA, T.Ya.PSPENICHNIKOVA and G.T.SUKHIKH, All-Union Research Centre for Maternal and Child Health,
Moscow 117815 USSR There is evidence that soluble products of activated im~nunocompetent cells may interfere with the reproductive process. Therefore their role in endometriosis-associated infertility is explored. Peritoneal fluid cell composition was analysed by direct flow cytofluorometry, using the FacScan device and monoclonal antibodies (Ortho Diagnostic System and Becton Dickinson), in infertile patients (minor external endometriosis as group I and pelvic adhesions as group 2). Cytotoxic effect of peritoneal fluid on donor sperms and target cells L-929, sensitive to tumor-necrotizing factor (TNF), was assessed. Spontaneous LPS- or opioid receptor ligand-induced TNF activity was evaluated in macrophage supernatant cultures. Altered macrophage/Lymphocyte ratio, and significantly increased content of DR+-macrophages were demonsarated in peritoneal fluid of endometriosis patients. Wide individual variability of sperm toxicity ( 20 to 60% ) and TNF activity (21 to 99% at 1:1 dilution) was demonstrated in peritoneal fluid of patients from both groups. There were no significant differences in the respective mean values between the groups. The results are suggestive of increased content-of cells, exoressing secondclass HLA antigens, particularly so in endometriosis patients. Activation of macrophage cells and their cytokine secretion may be the actual basis for gamete- and embryotoxic effects contributing to infertility in patients with endometriosis and pelvic adhesions. Endometriosis,Macrophages,Tumor necrosis factor.
P 293 E N D O M E T R I A L ANTIGENS ELICITING IN PATIENTS WITH ENDOMETRIOSIS.
AUTO-ANTIBODY
RESPONSES
S. MATHUR, D. GARZA, L.F. SMITH and H.O. WILLIAMSON, Dept. of Ob/Gyn, Medical University of South Carolina, Charleston, SC 29425. Endometrial antigens from patients with endometriosis and controls reactive against IgG, IgA or IgM antibodies in serum and peritoneal fluid of 7 endometriosis patients and 8 controls were identified by Western blot analysis. Serum and peritoneal fluid IgG from the patients reacted against endometrial antigens with molecular weight (MW) of 26 kD (present only in the endometrium from patients), 34 kD (present predominently in the patient endometrium) and 42 kD (present only in the patients' endometria). Serum and peritoneal fluid IgG from the controls failed to react against these antigens. Serum and peritoneal fluid IgA of both groups reacted against common endometrial antigens with MW of 25, 52, 58 and 66 kD and antigens with MW of 48, 60,100 and 120 kD detectable only in endometrium of patients. Three patients had serum IgA antibodies against patient endometrial antigen with MW 52 kD. Serum and peritoneal fluid IgM of both patients and controls reacted against common endometrial antigens with MW of 25, 46, 48, 56, 60, 66, 80 and 90 kD and antigen with MW of 52 kD detectable only in patient endometrium. It is concluded that endometrial antibody response belonging to IgG isotype is relevant to endometrial autoimrrunity in patients with endometriosis and that IgG antibodies are directed against antigens with MW 26, 34 and 42 kD found only in patient endometrium. Key words: Autoimmunity,endometriosis,endometrialantigens,endometrialantibodies