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Engineered protein kinases3 which can utilize modified nucleotide try-phosphate substrates
Mitokor N° W0 98/38335, PCT.
The present invention relates to methods of detecting genetic mutations in mitochondrial cytochrome oxidase c genes that segregate with Alzheimer's disease and methods for determining the amount of heteroplasmy of mitochondrial nucleic acid.
gent ; crosslinked, immobilized enzyme crystals (CLECs) made by this met h o d ; lyophilization of CLECs made by this method ; lyophilized crosslinked immobilized CLECs and a method of making a desired product by means of a reaction catalyzed by a CLEC or set of CLECs.
(...)
Therapies genique el cellu/aire > Compos~s glycerolipidiques utiles pour le transfert d'une susbstance active dans une cellule cible (R Bischoff et al, FR) Transgene SA et les inventeurs N° WO 98/34910, PCT.
Syst#.me de delivrance > Retroviral vectors, method for the production and the use thereof for gene transfer in CD4-positive cells (K Cichutek and J Stitz, DE) Bundesrepublik Deutschland, Pr~.sidenten des PauI-Ehrlich-lnstituts and the inventors N° WO 98/38325, PCT.
The invention relates to the production and use of retroviral vectors for cell-type specific gene transfer, specially to a production method of rerroviral vectors containing capsid particles of murine leukemia virus (MLV) and envelope proteins of human immunodeficiency viruses (H1V) or simian immunodeficiency viruses (SIV). Said vectors can be used for gene transfer in selected cell types, specially in CD4-positive mammal cells.
CHIMIE-GENIE ENZYIVIATIQUE Enzymes > Use of crosslinked crystals as a novel form of enzyme immobilization Vertex Pharmaceuticals Inc N° 861888, EE
i
A method of immobilizing an enzyme by forming crystals of the enzyme and, generally, also crosslinking the resulting crystals throught the use of a bifunctional rea58 BIOFUTUR183 • N0vembre1998
> Engineered protein kinases which can utilize modified nucleotide tryphosphate substrates {K Shokat, NJ, USA) Princetown University and the inventor N° W0 98/35048, PC].
Engineered protein kinases which can utilize modified nucleotide triphosphate substrates that are not readily utilized by the wild-type forms of those enzymes, and methods of making and using them. Modified nucleotides triphosphate substrates and methods of making and using them. Methods for using such engineered kinases and such modified substrates to identi~" which protein substrates the kinases act upon, to measure the extent of such action, and to determine if test compounds can modulate such action. (...) > Beta-glucosidases, methods for obtaining same, preparations containing said beta-glucosidases and uses thereof (M-P le Traon-Masson et P Pellerin, FR) G i s t - B r o c a d e s BV, Inra et les inventeurs N° WO 98/38316, PCT.
The invention provides substantially pure peptides having the following amino acid sequence : Giu-AlaTyr-Gln-Xaa1-Tyr-Leu-ValThr-Glu-Pro-Asn-Xaa2-Gly, wherein Xaal and Xaa2 may be a w encodable amino acid ; Giu-Ala-Tyr-GlnXaa 1-Tyr-keu-Val-Thr-gluPro-Asn-Xaa2-Gly or NH 2Leu Set Val Ser Phe Pro His Tyr Val Gly Asp Leu Pro lie Tyr Tyr Asp Tyr Leu -- COO, and beta-glucosidases comprising parts having the said amino acid sequences. Also provided are the uses of said peptides for design probes to detect, isolate and/or amplify' nucleic acid sequences coding for the said beta-D-glucosidases, expression constructs, and transformed host cells
capable of producing the said beta-D-glucosidase in amounts higher than usual. Also uses of the said beta-Dglucosidases are claimed in processes of modifying aroma precursor compounds and/or anthocyanidin-glucosides, in processes of making food, feed or beverage. The beta-D-glucosidases according to the invention are especially suitable for use in making wine and champagne. > Method for producing cellulose deriva'dves (Y Noguchi and M Kamachi, JP) N o v o Nordisk A/S and the inventors N° W0 98/38328, PCT.
Pulp is treated with a hemicellulase, eg a xylanase such as that derived from Bacillus sp SD902, prior to being chemically modified. This results in excellent cellulose derivatives that could not be obtained by any conventional methods. (...)
Fermentation > Method for producing an oxide with a fermentalion process (M Yoshida et al, JP) Fujisawa P h a r m a c e u t i c a l Co Ltd and the inventors N° WO 98/33885, PCT.
In a method for producing an oxide which comprises cultivating a strain of microorganism of the genus Gluconobacter, the genus Acetobacter, the genus Pseudogluconobacter, the genus Pseudomonas, the genus Corynebacterium, or the genus Erwinia to oxidize a substrate in a culture medium, an assimilable carbon source other than the substrate is admixed in the medium. (...) > Method for acquiring grain-shaped growth of a microorganism in a reactor (J Heijnen and M Van Loosdrecht, NL) Technische Universiteit Delft and the inventors N° WO 98/37027, PCT. The inventionrelates to a me-
thod of acquiring granular growth of a microorganism in a reactor containing a liquid medium. Surprisingly, according to the invention, aerobic microorganisms also can be induced to granular growth by maintaining specific culture cdinditions. (...)
ENVIRONNEMENT t NERGIE Bi0pesticides > Novel Bacillus thurinisolates active against sucking insects (S Riazuddin, PK)
giensis
Calgene Inc and the inventor N° W0 98/35046, PCT.
The subject invention concerns novel Bacillus thuringiensis strains containing parasporal proteins with pesticidal properties against witheft}; aphid, jassid, and possibly other sucking insects of agronomic importance, and peptide sequences to these proteins that can be used to obtain structural genes. (...)
TECHNOLOGIE Anaiyse du ger~ome > Z-chromosomal markers derived from chicken (Gallus domesticus) and use thereof in chromosomal mapping (A Ponce de Leon et al, MA, USA) University of M a s s a c h u setts, Institution of Higher E d u c a t i o n of t h e C o m m o n w e a l t h of M a s s a c h u setts, A m h e r t s C a m p u s and the inventors N° W0 98/37243, POT.
Development of a chicken (Gallus domesticus) Z-chromosome-specific DNA library in a phage vector, by means of chromosome microisolation and microcloning. The chromosomal origin, specificity and purity was evaluated by fluorescent in situ hybridization (FISH) on chicken metaphases. (...) > Cross-species chromosome painting (M Ferguson-Smith et al, GB, DE} Cambridge University Technical Services Lt and the inventors N° WO 98/38333, PCT.
The invention provides methods and reagents for detecting a chromosomal aberration in an animal chromosome or karyotype. One or more detectably-labeled chromosome-specific probes from a first animal species are hybridized to chromosomes of a second animal species. (...)
The present invention relates to methods of detecting genetic mutations in mitochondrial cytochrome oxidase c genes that segregate with Alzheimer's disease and methods for determining the amount of heteroplasmy of mitochondrial nucleic acid.
gent ; crosslinked, immobilized enzyme crystals (CLECs) made by this met h o d ; lyophilization of CLECs made by this method ; lyophilized crosslinked immobilized CLECs and a method of making a desired product by means of a reaction catalyzed by a CLEC or set of CLECs.
(...)
Therapies genique el cellu/aire > Compos~s glycerolipidiques utiles pour le transfert d'une susbstance active dans une cellule cible (R Bischoff et al, FR) Transgene SA et les inventeurs N° WO 98/34910, PCT.
Syst#.me de delivrance > Retroviral vectors, method for the production and the use thereof for gene transfer in CD4-positive cells (K Cichutek and J Stitz, DE) Bundesrepublik Deutschland, Pr~.sidenten des PauI-Ehrlich-lnstituts and the inventors N° WO 98/38325, PCT.
The invention relates to the production and use of retroviral vectors for cell-type specific gene transfer, specially to a production method of rerroviral vectors containing capsid particles of murine leukemia virus (MLV) and envelope proteins of human immunodeficiency viruses (H1V) or simian immunodeficiency viruses (SIV). Said vectors can be used for gene transfer in selected cell types, specially in CD4-positive mammal cells.
CHIMIE-GENIE ENZYIVIATIQUE Enzymes > Use of crosslinked crystals as a novel form of enzyme immobilization Vertex Pharmaceuticals Inc N° 861888, EE
i
A method of immobilizing an enzyme by forming crystals of the enzyme and, generally, also crosslinking the resulting crystals throught the use of a bifunctional rea58 BIOFUTUR183 • N0vembre1998
> Engineered protein kinases which can utilize modified nucleotide tryphosphate substrates {K Shokat, NJ, USA) Princetown University and the inventor N° W0 98/35048, PC].
Engineered protein kinases which can utilize modified nucleotide triphosphate substrates that are not readily utilized by the wild-type forms of those enzymes, and methods of making and using them. Modified nucleotides triphosphate substrates and methods of making and using them. Methods for using such engineered kinases and such modified substrates to identi~" which protein substrates the kinases act upon, to measure the extent of such action, and to determine if test compounds can modulate such action. (...) > Beta-glucosidases, methods for obtaining same, preparations containing said beta-glucosidases and uses thereof (M-P le Traon-Masson et P Pellerin, FR) G i s t - B r o c a d e s BV, Inra et les inventeurs N° WO 98/38316, PCT.
The invention provides substantially pure peptides having the following amino acid sequence : Giu-AlaTyr-Gln-Xaa1-Tyr-Leu-ValThr-Glu-Pro-Asn-Xaa2-Gly, wherein Xaal and Xaa2 may be a w encodable amino acid ; Giu-Ala-Tyr-GlnXaa 1-Tyr-keu-Val-Thr-gluPro-Asn-Xaa2-Gly or NH 2Leu Set Val Ser Phe Pro His Tyr Val Gly Asp Leu Pro lie Tyr Tyr Asp Tyr Leu -- COO, and beta-glucosidases comprising parts having the said amino acid sequences. Also provided are the uses of said peptides for design probes to detect, isolate and/or amplify' nucleic acid sequences coding for the said beta-D-glucosidases, expression constructs, and transformed host cells
capable of producing the said beta-D-glucosidase in amounts higher than usual. Also uses of the said beta-Dglucosidases are claimed in processes of modifying aroma precursor compounds and/or anthocyanidin-glucosides, in processes of making food, feed or beverage. The beta-D-glucosidases according to the invention are especially suitable for use in making wine and champagne. > Method for producing cellulose deriva'dves (Y Noguchi and M Kamachi, JP) N o v o Nordisk A/S and the inventors N° W0 98/38328, PCT.
Pulp is treated with a hemicellulase, eg a xylanase such as that derived from Bacillus sp SD902, prior to being chemically modified. This results in excellent cellulose derivatives that could not be obtained by any conventional methods. (...)
Fermentation > Method for producing an oxide with a fermentalion process (M Yoshida et al, JP) Fujisawa P h a r m a c e u t i c a l Co Ltd and the inventors N° WO 98/33885, PCT.
In a method for producing an oxide which comprises cultivating a strain of microorganism of the genus Gluconobacter, the genus Acetobacter, the genus Pseudogluconobacter, the genus Pseudomonas, the genus Corynebacterium, or the genus Erwinia to oxidize a substrate in a culture medium, an assimilable carbon source other than the substrate is admixed in the medium. (...) > Method for acquiring grain-shaped growth of a microorganism in a reactor (J Heijnen and M Van Loosdrecht, NL) Technische Universiteit Delft and the inventors N° WO 98/37027, PCT. The inventionrelates to a me-
thod of acquiring granular growth of a microorganism in a reactor containing a liquid medium. Surprisingly, according to the invention, aerobic microorganisms also can be induced to granular growth by maintaining specific culture cdinditions. (...)
ENVIRONNEMENT t NERGIE Bi0pesticides > Novel Bacillus thurinisolates active against sucking insects (S Riazuddin, PK)
giensis
Calgene Inc and the inventor N° W0 98/35046, PCT.
The subject invention concerns novel Bacillus thuringiensis strains containing parasporal proteins with pesticidal properties against witheft}; aphid, jassid, and possibly other sucking insects of agronomic importance, and peptide sequences to these proteins that can be used to obtain structural genes. (...)
TECHNOLOGIE Anaiyse du ger~ome > Z-chromosomal markers derived from chicken (Gallus domesticus) and use thereof in chromosomal mapping (A Ponce de Leon et al, MA, USA) University of M a s s a c h u setts, Institution of Higher E d u c a t i o n of t h e C o m m o n w e a l t h of M a s s a c h u setts, A m h e r t s C a m p u s and the inventors N° W0 98/37243, POT.
Development of a chicken (Gallus domesticus) Z-chromosome-specific DNA library in a phage vector, by means of chromosome microisolation and microcloning. The chromosomal origin, specificity and purity was evaluated by fluorescent in situ hybridization (FISH) on chicken metaphases. (...) > Cross-species chromosome painting (M Ferguson-Smith et al, GB, DE} Cambridge University Technical Services Lt and the inventors N° WO 98/38333, PCT.
The invention provides methods and reagents for detecting a chromosomal aberration in an animal chromosome or karyotype. One or more detectably-labeled chromosome-specific probes from a first animal species are hybridized to chromosomes of a second animal species. (...)