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Enhancement of protein content in “(“Kluyveromyces marxianus”)” biomass produced on cheese whey lactose
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Abstracts / Journal of Biotechnology 208 (2015) S5–S120
Enhancement of protein content in “(“Kluyveromyces marxianus”)” biomass produced on cheese whey lactose Jekaterina Lukjanenko, Kristiana Kovtuna, Mara Grube ∗ , Armands Vigants Environment, Bioenergetics and Biotechnology Competence Centre, Riga, Latvia E-mail address: [email protected] (M. Grube). The non-conventional food grade yeasts Kluyveromyces marxianus can be used for bioconversion of lactose containing cheese whey permeate to bioethanol and yeast biomass. Yeast biomass itself is valuable product with application as animal feed, food additive and as a raw material for yeast extract production. The composition of yeast biomass can vary depending on the cultivation conditions and substrate composition. The current research deals with infrared spectroscopy study of K. marxianus DSM 5422 cell protein content during the lactose fermentation. The protein content in biomass increases by increasing the fermentation temperature, while impact of lactose amendment depends on the concentration range. The 2-way factorial design experiments reveal the interaction of both factors. K. marxianus cells grown on cheese whey permeate had low protein content (about 30% DW) probably due to nitrogen limitation. The addition of ammonium salts to cheese whey permeates showed minor effect on biomass yield while significantly increased the protein content (up to 45% DW) in cells. Positive linear correlation between ammonium sulfate concentration in fermentation medium and protein content in K. marxianus cells was found in the concentration range of 0 to 2 g/l of (NH4 )2 SO4 . The changes of protein content were observed at different fermentation stages. Acknowledgments: This study was supported by ERAF project Nr. KC/2.1.2.1.1/10/01/006, L-KC-11-0005. http://dx.doi.org/10.1016/j.jbiotec.2015.06.232 The correlation between water temperature from a trout farm and blood biochemical parameters of fish Elena Mitranescu 1,∗ , Violeta Simion 2 , Alexandru Lataretu 1 , Monica Pirvu 2 , Laurentiu Tudor 1
in summer compared to winter months, increases associated with nutrient intake offered or existing, and with reduced physical activity consistent with photoperiodicity and indices of external temperature and water. The activity of blood serum transaminase increased noticeably during colder months, attesting the existence of additional requests to the hepatic metabolism in correlation with seasonal alternation and/or temperature. http://dx.doi.org/10.1016/j.jbiotec.2015.06.233 The evaluation of welfare in a dairy farm in South-eastern Romania Elena Mitranescu 1,∗ , Violeta Simion 2 , Aneta Laura Tudor 2 , Laurentiu Tudor 1 1
Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 Faculty of Veterinary Medicine, University of “Spiru - Haret”, Bucharest, Romania E-mail address: [email protected] (E. Mitranescu). In Romania consumers of animal products are becoming increasingly interested in optimizing farm animal welfare. The purpose of this paper is to verify the applicability and objectivity in a dairy farm in Romania integrative numeric system – 35 YEARS L – welfare assessment. The study was done in a shelter with dairy Romanian Black Spotted breed with different ages and different physiological status. According to ANI 35 L welfare assessment system was based on the 5 groups of factors. Within each group of factors is estimated that more items will be marked separately as the general data to obtain the score for the whole group of factors. Following evaluations have revealed the following: Results: overall score of group I of factors “freedom of movement” is 5 points; overall score of Group II of factors “social interaction” is 5.5 points; overall score factors of group III “type and characteristics of the floor and outdoor surfaces” is 4 points; overall score of group IV of factors “lighting, air quality and noise” is 6 points; overall score factors of group V “care and maintenance conditions” is 2.5 points. According ANI 35L between −9 and +46 points, it appears that animal welfare assessed as average total score of the five factors is 23 points. The researches demonstrate the applicability and objectivity in a dairy farm in Romania for integrative numeric system welfare assessment.
1
Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 Faculty of Veterinary Medicine, University of “Spiru - Haret”, Bucharest, Romania E-mail address: [email protected] (E. Mitranescu). Intensive rearing of trout is dependent on both the environmental parameters, in particular the significant variation in water temperature and oxygen concentration in water and density, handling, transport and fish health. In a trout farm from Vâlcea areas were monitoring the temperature and oxygen concentration in the water during the winter months (December, January, February) and summer months (June, July, August). From the blood samples was analysed: alanine aminotransferase, urea, creatinine, albumin, glucose and total protein by using the 8008 IDEXX Vettest. The data obtained have been compared with the values from specialized references and were statistically processed and interpreted. Synthetic and comparative analysis of the major components constituting the metabolic profile revealed variations correlated with season, water temperature and variations of photoperiod. The total serum protein content revealed a moderate but steady growth
http://dx.doi.org/10.1016/j.jbiotec.2015.06.234 Consumer’s perception referring to buying and consumption of ecological food products Ioana Anda Milin ∗ , Andreea Mihaela Radac, Iuliana Ioana Merce, Ramona Ciolac Department of Economy, USAMVB “Regele Mihai I al Romaniei” Timisoara, Romania E-mail address: anda [email protected] (I.A. Milin). Biological agriculture is considered a viable solution that solves the agriculture’s negative impact on environment and on food quality. Referring to ecological products demand at world level, this is in a permanent increasing. Foods produced by ecological agriculture have always been more expensive that those produced in a conventional way, and this fact has been considered many times an obstacle in developing this kind of agriculture. The study proposed in the paper fits in the field of existing preoccupation worldwide and at national level because the production, processing, and distribution and consumption of agricultural and
Abstracts / Journal of Biotechnology 208 (2015) S5–S120
Enhancement of protein content in “(“Kluyveromyces marxianus”)” biomass produced on cheese whey lactose Jekaterina Lukjanenko, Kristiana Kovtuna, Mara Grube ∗ , Armands Vigants Environment, Bioenergetics and Biotechnology Competence Centre, Riga, Latvia E-mail address: [email protected] (M. Grube). The non-conventional food grade yeasts Kluyveromyces marxianus can be used for bioconversion of lactose containing cheese whey permeate to bioethanol and yeast biomass. Yeast biomass itself is valuable product with application as animal feed, food additive and as a raw material for yeast extract production. The composition of yeast biomass can vary depending on the cultivation conditions and substrate composition. The current research deals with infrared spectroscopy study of K. marxianus DSM 5422 cell protein content during the lactose fermentation. The protein content in biomass increases by increasing the fermentation temperature, while impact of lactose amendment depends on the concentration range. The 2-way factorial design experiments reveal the interaction of both factors. K. marxianus cells grown on cheese whey permeate had low protein content (about 30% DW) probably due to nitrogen limitation. The addition of ammonium salts to cheese whey permeates showed minor effect on biomass yield while significantly increased the protein content (up to 45% DW) in cells. Positive linear correlation between ammonium sulfate concentration in fermentation medium and protein content in K. marxianus cells was found in the concentration range of 0 to 2 g/l of (NH4 )2 SO4 . The changes of protein content were observed at different fermentation stages. Acknowledgments: This study was supported by ERAF project Nr. KC/2.1.2.1.1/10/01/006, L-KC-11-0005. http://dx.doi.org/10.1016/j.jbiotec.2015.06.232 The correlation between water temperature from a trout farm and blood biochemical parameters of fish Elena Mitranescu 1,∗ , Violeta Simion 2 , Alexandru Lataretu 1 , Monica Pirvu 2 , Laurentiu Tudor 1
in summer compared to winter months, increases associated with nutrient intake offered or existing, and with reduced physical activity consistent with photoperiodicity and indices of external temperature and water. The activity of blood serum transaminase increased noticeably during colder months, attesting the existence of additional requests to the hepatic metabolism in correlation with seasonal alternation and/or temperature. http://dx.doi.org/10.1016/j.jbiotec.2015.06.233 The evaluation of welfare in a dairy farm in South-eastern Romania Elena Mitranescu 1,∗ , Violeta Simion 2 , Aneta Laura Tudor 2 , Laurentiu Tudor 1 1
Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 Faculty of Veterinary Medicine, University of “Spiru - Haret”, Bucharest, Romania E-mail address: [email protected] (E. Mitranescu). In Romania consumers of animal products are becoming increasingly interested in optimizing farm animal welfare. The purpose of this paper is to verify the applicability and objectivity in a dairy farm in Romania integrative numeric system – 35 YEARS L – welfare assessment. The study was done in a shelter with dairy Romanian Black Spotted breed with different ages and different physiological status. According to ANI 35 L welfare assessment system was based on the 5 groups of factors. Within each group of factors is estimated that more items will be marked separately as the general data to obtain the score for the whole group of factors. Following evaluations have revealed the following: Results: overall score of group I of factors “freedom of movement” is 5 points; overall score of Group II of factors “social interaction” is 5.5 points; overall score factors of group III “type and characteristics of the floor and outdoor surfaces” is 4 points; overall score of group IV of factors “lighting, air quality and noise” is 6 points; overall score factors of group V “care and maintenance conditions” is 2.5 points. According ANI 35L between −9 and +46 points, it appears that animal welfare assessed as average total score of the five factors is 23 points. The researches demonstrate the applicability and objectivity in a dairy farm in Romania for integrative numeric system welfare assessment.
1
Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 Faculty of Veterinary Medicine, University of “Spiru - Haret”, Bucharest, Romania E-mail address: [email protected] (E. Mitranescu). Intensive rearing of trout is dependent on both the environmental parameters, in particular the significant variation in water temperature and oxygen concentration in water and density, handling, transport and fish health. In a trout farm from Vâlcea areas were monitoring the temperature and oxygen concentration in the water during the winter months (December, January, February) and summer months (June, July, August). From the blood samples was analysed: alanine aminotransferase, urea, creatinine, albumin, glucose and total protein by using the 8008 IDEXX Vettest. The data obtained have been compared with the values from specialized references and were statistically processed and interpreted. Synthetic and comparative analysis of the major components constituting the metabolic profile revealed variations correlated with season, water temperature and variations of photoperiod. The total serum protein content revealed a moderate but steady growth
http://dx.doi.org/10.1016/j.jbiotec.2015.06.234 Consumer’s perception referring to buying and consumption of ecological food products Ioana Anda Milin ∗ , Andreea Mihaela Radac, Iuliana Ioana Merce, Ramona Ciolac Department of Economy, USAMVB “Regele Mihai I al Romaniei” Timisoara, Romania E-mail address: anda [email protected] (I.A. Milin). Biological agriculture is considered a viable solution that solves the agriculture’s negative impact on environment and on food quality. Referring to ecological products demand at world level, this is in a permanent increasing. Foods produced by ecological agriculture have always been more expensive that those produced in a conventional way, and this fact has been considered many times an obstacle in developing this kind of agriculture. The study proposed in the paper fits in the field of existing preoccupation worldwide and at national level because the production, processing, and distribution and consumption of agricultural and