Matrix Biology 22 (2003) 93–96
Erratum
Erratum to ‘‘Fibrillin-1 and fibrillin-2 in human embryonic and early fetal development’’ wMatrix Biol. 21 (2002) 637–646x 夞 Fabio Quondamatteoa,*, Dieter P. Reinhardtb, Noe L. Charbonneauc, Gabriele Pophala, Lynn Y. Sakaic, Rainer Herkena a
¨ ¨ Department of Histology, University of Gottingen, Kreuzbergring 36, Gottingen D-37075, Germany b ¨ ¨ Department of Medical Molecular Biology, University of Lubeck, Lubeck, Germany c Research Department, Shriners Hospital for Children, Portland, OR, USA
The publisher regrets that in the printing of the above article Figs. 1–13 were printed in black and white. We regret any embarrassment caused to those concerned. Figs. 1–13 are printed in colour on the following pages.
夞 Original PII: S0945-053X(02)00100-2 *Corresponding author. Tel.: q49-551-398982; fax: q49-551-397067. E-mail address:
[email protected] (F. Quondamatteo). 0945-053X/03/$30.00 䊚 2003 Elsevier Science B.V.yInternational Society of Matrix Biology. All rights reserved. PII: S 0 9 4 5 - 0 5 3 X Ž 0 3 . 0 0 0 1 6 - 7
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Fig. 1. Lung anlage 7th GW immunostaining for fibrillin-1. Subepithelial staining (black arrows) at the epithelial–mesenchymal interface in the developing bronchial anlage. Some of the segments of the bronchial anlage do not show any staining at their epithelial–mesenchymal interface (open arrows). *Lung stroma 200=. Fig. 2 Lung anlage 7th GW immunostaining for fibrillin-2. This picture shows a pattern comparable to fibrillin-1, depicted in Fig. 1. Black arrows, immunostaining for fibrillin-2 of the bronchi located at the epithelial–mesenchymal interface. Open arrow, segments of the bronchial anlage devoid of fibrillin-2 immunostaining. *Lung stroma, 200=. Fig. 3 Lung anlage 12th GW immunostaining for fibrillin-1. Staining (black arrows) at the epithelial–mesenchymal interface of the developing bronchial anlagen (b). The immunostaining does not completely surround the entire bronchial anlage but some segments of the bronchi are devoid of it at their mesenchymal–epithelial interface (open arrows). *Lung stroma, 200=. Fig. 4 Lung anlage 12th GW immunostaining for fibrillin-2. This picture shows a pattern comparable to fibrillin-1, depicted in Fig. 3. Black arrows, immunostaining for fibrillin-2 of the bronchi located at the epithelial–mesenchymal interface; open arrow, segments of the bronchial anlage devoid of fibrillin-2 immunostaining. *Lung stroma, 200=. Fig. 5 Heart anlage 5th GW, immunostaining for fibrillin-1. Staining is visible in the matrix of the endocardial cushions (*). 80=. Fig. 6 Heart anlage 5th GW, immunostaining for fibrillin2. Staining is visible in the matrix of the endocardial cushions (*). 80=. Fig. 7 Cartilaginous rib anlage 9th GW, immunostaining for fibrillin-1. Positive staining is present in the cartilaginous matrix (*) and in the perichondrial layer (arrows). 350=. Fig. 8 Cartilaginous rib anlage 9th GW, immunostaining for fibrillin-2. Positive staining is present in the perichondrial layer (arrows), while the cartilaginous matrix (*) is negative. 350=. Fig. 9 Ossificating rib anlage 11th GW, immunostaining for fibrillin-1. Positive staining is present in the perichondrial layer (arrow). The cartilaginous matrix is stained (*) with the exception of the hypertrophic zone (h). 80=. Fig. 10 Ossificating rib anlage 11th GW, immunostaining for fibrillin-2. Positive staining is present in the perichondrial layer (arrow), the cartilaginous matrix (*) is negative. H, hypertrophic zone. 80=. Fig. 11. (a) Notochord 5th GW, immunostaining for fibrillin-1. Staining is neither visible in the notochord (n), nor in the notochordal sheath (arrow) nor in the perichordal mesenchyme (*) 500=. (b) Notochord 5th GW, immunostaining for fibrillin-2. Staining is present in the notochordal sheath (arrow) and in the perichordal mesenchyme (*). No staining is visible in the notochord (n). 500=. (c) Vertebral anlage 8th GW, immunostaining for fibrillin-1. Positive staining is present in the matrix of the vertebral body anlagen (vb), while the anlagen of the intervertebral discs (*) are negative. Positive staining is also present in the anlagen of the vertebral ligaments (arrowheads) and, within the notochord (n), in the notochordal channel. Adjacent to the vertebral anlage, staining is visible in the leptomeningeal sheath (black arrows) while the neuroepithelium (ne) is negative, 100=. (d) Higher magnification of the notochord of the 8th GW, immunostaining for fibrillin-1. Positive staining is present in the notochordal channel (arrow), 400=. (e) Vertebral anlage 8th GW, immunostaining for fibrillin-2. The distribution of the fibrillin-2 immunostaining is analogous to that of fibrillin-1 which is depicted in (c). Immunostaining in the vertebral body anlagen (vb) and ligaments (arrowheads). Asterisks, anlagen of the intervertebral discs. Ne, neuroepithelium. Black arrows, immunostaining of the leptomeningeal sheath adjacent to the vertebral anlage, 100=. (f) Higher magnification of the notocord of the 8th GW, immunostaining for fibrillin-2 of the notochordal channel (arrow), 300=. Fig. 12(a) Liver anlage 5th GW, immunostaining for fibrillin-l. Clear staining is present in the lining of early intrahepatic parenchymal microvessels (arrow). H, hepatocytes, 400=. (b) Parenchyme of the liver anlage of the 11th GW, immunostaining for fibrillin-l. No staining is visible in the lining of parenchymal microvessels (arrows). H, hepatocytes, 400=. (c) Liver anlage 11th GW, immunostaining for fibrillin-1. Staining is visible only in the matrix of the portal anlagen (arrows). V, vein of a portal space, 300=. (d) Liver anlage 5th GW, immunostaining for fibrillin-2. No staining is present in the lining of the early intrahepatic parenchymal micro vessels (arrows). H, hepatocytes, 500=. Fig. 13 (a) Kidney anlage 5th GW, immunostaining for fibrillin-1. Clear staining is visible in the basement membrane zones of glomerular microvessels (arrowheads) and in the basement membrane zones of tubuli (arrows). Weak interstitial staining of the stroma of the kidney anlage (*) is also visible. G, glomeruli, 400=. (b) Kidney anlage 5th GW, immunostaining for fibrillin-2. Clear staining is visible only in the tubular basement membrane zones (arrows), but not in the glomcruli (g). Very weak staining is present in the stroma of the kidney anlage (*). G, glomeruli, 400=.