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Erratum to “Phenotypic and genotypic characterization of drug-resistant Mycobacterium tuberculosis strains” [Diagn Microbiol Infect Dis 62 (2008) 199–204]
Available online at www.sciencedirect.com
Diagnostic Microbiology and Infectious Disease 65 (2009) 462 www.elsevier.com/locate/diagmicrobio
Erratum
Erratum to “Phenotypic and genotypic characterization of drug-resistant Mycobacterium tuberculosis strains” [Diagn Microbiol Infect Dis 62 (2008) 199–204] Wanessa Trindade Clementea , Stella Sala Soares Limab , Moises Palacic , Márcia S.N. Silvad , Vivian F. Sumnienski Rodriguesd , Elis R. Dalla Costad , Lia Possuelod , Patrícia Izquierdo Cafruned , Fabíola Karla Ribeirod , Harisson M. Gomese , José Carlos Serufof a
Departamento de Propedêutica Complementar da Faculdade de Medicina da Universidade Federal de Minas Gerais. Av. Alfredo Balena, 190, Santa Efigênia, Belo Horizonte, MG, CEP 30130-100, Brazil b Comissão de Controle de Infecção Hospitalar do Hospital das Clínicas da Universidade Federal de Minas Gerais. Av. Alfredo Balena, 110, Santa Efigênia, Belo Horizonte, MG, CEP 30130-100, Brazil c Núcleo de Doenças Infecciosas da Universidade Federal do Espírito Santo. Av. Marecha Campos, 1468, Maruípe, Vitória, ES, CEP 29040-091, Brazil d Universidade Luterana do Brasil (ULBRA/RS). Av. Farroupilha, 8001, São José, Canoas, RS, CEP 92425-900, Brazil e Instituto Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, CEP 21040-900, Brazil f Departamento de Clínica Médica da Faculdade de Medicina da Universidade Federal de Minas Gerais. Av. Alfredo Balena, 190, Santa Efigênia, Belo Horizonte, MG, CEP 30130-100, Brazil
After publication of the above mentioned article, a revised Table 3 was provided and is included below. Furthermore, on page 203 under Section 4.3 Pyrazinamide, the sentence reading “Our study observed that around 60% of PZA-resistant strains had pncA gene mutations from positions 136 to 188, and although pncA is a small gene, mutations in
various positions of its structure have been found (Zhang and Mitchinson, 2003).”, should read, “Our study observed that around 60% of PZA-resistant strains had pncA gene mutations from positions 99 to 175, and although pncA is a small gene, mutations in various positions of its structure have been found (Zhang and Mitchinson, 2003).”
Table 3 Gene mutations associated with anti-TB drug resistance Drug resistant strains (n)
Resistant strains tested (n)⁎
Mutation type
Base change
INH (21)
20
katG
315 S315T 299 G299S −9 −12 −30 −39 – 531
ahpC
RIF (17)
14
inhA rpoB
PZA (10)
8
pncA
EMB (10)
7
embB
Nonspecific codon 152 175 136 99 306
⁎ Some strains were not recovered by subcultures. DOI of original article: 10.1016/j.diagmicrobio.2008.06.013. 0732-8893/$ – see front matter © 2009 Elsevier Inc. All rights reserved. 10.1016/j.diagmicrobio.2009.10.017
Mutant Strains AGC (Ser)→ACC (Thr) GGC(Gly)→AGC(Ser) G(-9)A C(-12)T C(-30)T C(-39)T – TCG (Ser)→TGG (Trp) TCG (Ser)→TGG (Leu) – Insertion T ATG (Met)→ATA (Ile) GAT (Asp)→AAT (Asn) TAC (Tyr)→TAG (stop) AUG (Met)→AUA (Ile) AUG (Met)→AUC (Ile) AUG (Met)→AUU (Ile) AUG (Met)→GUG (Val)
14 1 1 1 1 1 0 2 6 3 2 1 1 1 2 1 1 1
Mutation not found 5 16
20 3
3
2
Diagnostic Microbiology and Infectious Disease 65 (2009) 462 www.elsevier.com/locate/diagmicrobio
Erratum
Erratum to “Phenotypic and genotypic characterization of drug-resistant Mycobacterium tuberculosis strains” [Diagn Microbiol Infect Dis 62 (2008) 199–204] Wanessa Trindade Clementea , Stella Sala Soares Limab , Moises Palacic , Márcia S.N. Silvad , Vivian F. Sumnienski Rodriguesd , Elis R. Dalla Costad , Lia Possuelod , Patrícia Izquierdo Cafruned , Fabíola Karla Ribeirod , Harisson M. Gomese , José Carlos Serufof a
Departamento de Propedêutica Complementar da Faculdade de Medicina da Universidade Federal de Minas Gerais. Av. Alfredo Balena, 190, Santa Efigênia, Belo Horizonte, MG, CEP 30130-100, Brazil b Comissão de Controle de Infecção Hospitalar do Hospital das Clínicas da Universidade Federal de Minas Gerais. Av. Alfredo Balena, 110, Santa Efigênia, Belo Horizonte, MG, CEP 30130-100, Brazil c Núcleo de Doenças Infecciosas da Universidade Federal do Espírito Santo. Av. Marecha Campos, 1468, Maruípe, Vitória, ES, CEP 29040-091, Brazil d Universidade Luterana do Brasil (ULBRA/RS). Av. Farroupilha, 8001, São José, Canoas, RS, CEP 92425-900, Brazil e Instituto Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, CEP 21040-900, Brazil f Departamento de Clínica Médica da Faculdade de Medicina da Universidade Federal de Minas Gerais. Av. Alfredo Balena, 190, Santa Efigênia, Belo Horizonte, MG, CEP 30130-100, Brazil
After publication of the above mentioned article, a revised Table 3 was provided and is included below. Furthermore, on page 203 under Section 4.3 Pyrazinamide, the sentence reading “Our study observed that around 60% of PZA-resistant strains had pncA gene mutations from positions 136 to 188, and although pncA is a small gene, mutations in
various positions of its structure have been found (Zhang and Mitchinson, 2003).”, should read, “Our study observed that around 60% of PZA-resistant strains had pncA gene mutations from positions 99 to 175, and although pncA is a small gene, mutations in various positions of its structure have been found (Zhang and Mitchinson, 2003).”
Table 3 Gene mutations associated with anti-TB drug resistance Drug resistant strains (n)
Resistant strains tested (n)⁎
Mutation type
Base change
INH (21)
20
katG
315 S315T 299 G299S −9 −12 −30 −39 – 531
ahpC
RIF (17)
14
inhA rpoB
PZA (10)
8
pncA
EMB (10)
7
embB
Nonspecific codon 152 175 136 99 306
⁎ Some strains were not recovered by subcultures. DOI of original article: 10.1016/j.diagmicrobio.2008.06.013. 0732-8893/$ – see front matter © 2009 Elsevier Inc. All rights reserved. 10.1016/j.diagmicrobio.2009.10.017
Mutant Strains AGC (Ser)→ACC (Thr) GGC(Gly)→AGC(Ser) G(-9)A C(-12)T C(-30)T C(-39)T – TCG (Ser)→TGG (Trp) TCG (Ser)→TGG (Leu) – Insertion T ATG (Met)→ATA (Ile) GAT (Asp)→AAT (Asn) TAC (Tyr)→TAG (stop) AUG (Met)→AUA (Ile) AUG (Met)→AUC (Ile) AUG (Met)→AUU (Ile) AUG (Met)→GUG (Val)
14 1 1 1 1 1 0 2 6 3 2 1 1 1 2 1 1 1
Mutation not found 5 16
20 3
3
2