Erratum to ‘Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus’ J. Virol. Methods 49 (1994) 285–294

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Journal 03 Virological Methods Journal of Virological Methods 53 (1995) 273

Erratum

Erratum to ‘Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus’ J. Virol. Methods 49 (1994) 285-294 * V.M. Carn, R.P. Kitching *, J.M. Hammond, P. Chand ‘, J. Anderson, D.N. Black AFRC Institutefor Animal Health, Pit-bright Laboratory, Pirbright, Woking, Surrey, GU24 ONF, UK

Accepted 10 April 1994

Abstract The gene coding for the capripoxvirus structural protein P32 was cloned, expressed in Escherichia coli as a fusion protein with glutathione-S-transferase, and purified on glutathione Sepharose. An indirect enzyme linked immunosorbent assay (ELBA) using this antigen was developed to screen bovine sera for antibodies to capripoxvirus. Sequential serum samples from experimentally infected animals tested by ELISA and by virus neutralisation test (VNT) showed that the ELISA was more sensitive and detected antibodies to capripoxvirus earlier post-infection than the VNT. Keywords: Capripoxvirus; Recombinant antigen; ELISA, Antibody; Bovine

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* SSDI of the original article: 0166-0934(94)00054-K. * Corresponding author. ’ Present address: Dept. of Veterinary Pathology, CCS Haryana Agricultural University, Hisar 125004, India. 0166-0934/95/$09.50

0 1995 Elsevier Science B.V. All rights reserved

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