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Establishment of a human pulmonary adenocarcinoma cell line (PC84045) and its biological properties
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Abstract In Xuanwei County, Yunnan Province, lung cancer mortality is among China's highest. The former studies showed that indoor burning of "smoky" coal was an important cause for high incidence of Xuanwei lung cancer, but found no asl:Mi The smoking. sociation with matched case-control study was undertaken to examine the association with other risk factors such as smoking, familial history, cooking and so on. 110 cases of primary lung cancer newly local diagnosed was collected from hospitals between November, 1985 and Controls were inDecember, 1986. dividually matched with cases according to age (22 years), sex and village lived. 426 controls were interviewed. study All subjects came from the agricultural population in Xuanwei. Traditional stratification methods and conditional logistic regression were employed to examine for effect modification and to control confounding. Cooking and quantity of smoky coal used were found to be risk factors for Xuanwei lung cancer. The odds ratio (OR) and attribute risk (AR) associated with cooking were separately 2.7 and 46.8%. The analytic results showed that smoking was one of the risk factors of Xuanwei male lung cancer. But the strength of association was weaker (OR = 1.5, 95% CI = 1.01-2.25). No relation was found between passive smoking and female lung cancer. The association was observed for familial history of lung cancer (OR = 2.9, 95% CI = 1.66-5.14). A highly significant OR of 7.4 was for found history of chronic bronchitis. No relation was found about types of stove, dietary carotene and vitamine C, drinking, familial economic status and mental factor.
FEMALE
BRONCHOGENIC CARCINOMA AND MENSTRUATION
Analysis of 120 Cases He Giao, Deng Xiaohua, Huang Xiaoping, Department of Guangzhou Pathology, Chest Hospital. Abstract According statistical to the analysis of age, cell type, condition of childbearing and in relation to menstruation in 120 cases of female bronchogenic carcinoma, we found that there are four major characteristics to be noticed: 1. Most cases of female bronchogenic carcinoma occurred at the age group of 60-69 years (43%); it is 10 years older than that of our recent report (analysis of 880 cases of bronchogenic carcinoma including male and female patients);
2. The main cell types of female bronchogenic carcinoma is of adenocarcinoma (71%); 3. The menopause occurred mostly of 5052 years of age, 34% of total cases. Bronchogenic carcinoma develops &rally 10 years after menopause (56%). On the base of these four characteristics in female bronchogenic carcinoma and the observation of Chalon on the periodic change menstrual cycle, we proposed that the cases of age incidence in female bronchogenic carcinoma is rather older than that of male; which may be related with the menstrual periodic changes in bronchial mucoepithelium. In addition, the cell types in most cases is adenocarcinoma whether is also related to the effect of menstrual cyclic changes deserve further study. ESTABLISHMENT HUMAN PULMONARY ADENOCARCINOMA LINE CELL (PC84045) AND ITS BIOLOGICAL PROPERTIES
OF
A
Abstract Zhou Wenhua, Chen Xiurong, Ho Lijuan, Lo Ruixian, Guangzhou Medical and Health Research Institute. A pulmonary adenocarcinoma cell line (PC84045) was established in this laboratory by serial cultivation of cells collected from pleural fluid in a case of primary adenocarcinoma of lung. The cells appeared to be epitheloid with mosiac arrangement and pile-up growth. The cells proliferated steadily and rapidly with an average generation time of 24 hours, forming a mono-layer growth. Chromosome numbers of cells varied in a wide range (30-233) mostly resembling triploid. Karyotypic analysis showed various kinds of abnormal chromosomes and 3 chromosomal markers. Transplanted tumor growth resulted by inoculating the cells into Xrayirradiated weaning JCR mice, and the malignant cells could be seen in the lesion of the site. transplanted Polygonal epithelial cells similar to the original pleural fluid culture cells were also found in the tissue culture of the transplanted tumor tissue. Scanning electron microscopy of the actively growing cells revealed dense microvilli on the cell surface and transmitting electron microscopy occasionally showed close contact and desmosome-like linkage between cells. From the experiment it might be proved that the cell line was derived from malignant cells of pulmonary adenocarcinoma patient and preserved properties the of the original cancer cells.
Abstract In Xuanwei County, Yunnan Province, lung cancer mortality is among China's highest. The former studies showed that indoor burning of "smoky" coal was an important cause for high incidence of Xuanwei lung cancer, but found no asl:Mi The smoking. sociation with matched case-control study was undertaken to examine the association with other risk factors such as smoking, familial history, cooking and so on. 110 cases of primary lung cancer newly local diagnosed was collected from hospitals between November, 1985 and Controls were inDecember, 1986. dividually matched with cases according to age (22 years), sex and village lived. 426 controls were interviewed. study All subjects came from the agricultural population in Xuanwei. Traditional stratification methods and conditional logistic regression were employed to examine for effect modification and to control confounding. Cooking and quantity of smoky coal used were found to be risk factors for Xuanwei lung cancer. The odds ratio (OR) and attribute risk (AR) associated with cooking were separately 2.7 and 46.8%. The analytic results showed that smoking was one of the risk factors of Xuanwei male lung cancer. But the strength of association was weaker (OR = 1.5, 95% CI = 1.01-2.25). No relation was found between passive smoking and female lung cancer. The association was observed for familial history of lung cancer (OR = 2.9, 95% CI = 1.66-5.14). A highly significant OR of 7.4 was for found history of chronic bronchitis. No relation was found about types of stove, dietary carotene and vitamine C, drinking, familial economic status and mental factor.
FEMALE
BRONCHOGENIC CARCINOMA AND MENSTRUATION
Analysis of 120 Cases He Giao, Deng Xiaohua, Huang Xiaoping, Department of Guangzhou Pathology, Chest Hospital. Abstract According statistical to the analysis of age, cell type, condition of childbearing and in relation to menstruation in 120 cases of female bronchogenic carcinoma, we found that there are four major characteristics to be noticed: 1. Most cases of female bronchogenic carcinoma occurred at the age group of 60-69 years (43%); it is 10 years older than that of our recent report (analysis of 880 cases of bronchogenic carcinoma including male and female patients);
2. The main cell types of female bronchogenic carcinoma is of adenocarcinoma (71%); 3. The menopause occurred mostly of 5052 years of age, 34% of total cases. Bronchogenic carcinoma develops &rally 10 years after menopause (56%). On the base of these four characteristics in female bronchogenic carcinoma and the observation of Chalon on the periodic change menstrual cycle, we proposed that the cases of age incidence in female bronchogenic carcinoma is rather older than that of male; which may be related with the menstrual periodic changes in bronchial mucoepithelium. In addition, the cell types in most cases is adenocarcinoma whether is also related to the effect of menstrual cyclic changes deserve further study. ESTABLISHMENT HUMAN PULMONARY ADENOCARCINOMA LINE CELL (PC84045) AND ITS BIOLOGICAL PROPERTIES
OF
A
Abstract Zhou Wenhua, Chen Xiurong, Ho Lijuan, Lo Ruixian, Guangzhou Medical and Health Research Institute. A pulmonary adenocarcinoma cell line (PC84045) was established in this laboratory by serial cultivation of cells collected from pleural fluid in a case of primary adenocarcinoma of lung. The cells appeared to be epitheloid with mosiac arrangement and pile-up growth. The cells proliferated steadily and rapidly with an average generation time of 24 hours, forming a mono-layer growth. Chromosome numbers of cells varied in a wide range (30-233) mostly resembling triploid. Karyotypic analysis showed various kinds of abnormal chromosomes and 3 chromosomal markers. Transplanted tumor growth resulted by inoculating the cells into Xrayirradiated weaning JCR mice, and the malignant cells could be seen in the lesion of the site. transplanted Polygonal epithelial cells similar to the original pleural fluid culture cells were also found in the tissue culture of the transplanted tumor tissue. Scanning electron microscopy of the actively growing cells revealed dense microvilli on the cell surface and transmitting electron microscopy occasionally showed close contact and desmosome-like linkage between cells. From the experiment it might be proved that the cell line was derived from malignant cells of pulmonary adenocarcinoma patient and preserved properties the of the original cancer cells.