- Email: [email protected]
Ethanol fermentation system combined with membrane separator of pervaporation
Abstracts of the Articles Printed in Hakkokogaku Kaishi
577
Abstracts of the Articles Printed in Hakkokogaku Kaishi Vol. 64, No. 6 (1986)
Cultural Condition for Cell Mass Production of Aureobasidium pullulans f r o m Solvolysis pulping Waste Liquor. JUN HOSOKAWA, KAZUTOSHI Y0SHIHARA, M.A~HI NXSHPgA~tn, and HIROSHI K A m S H ~ (Government Industrial Research Institute
Sikoku, 2--3-3 Hananomiya-cho, Takamatsu-shi 761, Japan) Hakkokogaku 64: 473-477. 1986. The optimum cultural conditions were investigated for the cell mass production of strain A. pullulans IFO 6353 which has found to be the most promising organism that uses xylan in the solvolysis pulping waste liquor. The optimum cultural conditions were 29°C, initial pH 6.0-6.5. Ammonium sulfate and Lasparagine were suitable as supplementary nitrogen sources. The optimum concentrations of supplemental nutrients were 0.5% ammonium sulfate, 0.5-1.0% potassium dihydrogenphosphate, and 0. 1% magnesium sulfate. Further, the addition of yeast extract greatly increased the cell mass production. The cell mass reached 7.5 g per l of medium after 3 days under the optimum conditions (yeast extract, 0.25%). After 3 days of cultivation, A. pullulans IFO 6353 had used about 70% of the mono-, oligo-, and poly-saccharides in the waste liquor as one of the carbon sources.
contributor to the lytic activity on ftmgal cell walls. Optimum temperature and pH of the lytic activity were 45°C and 6.0-7.0, respectively. The lytie activity was stable at pH 5.0-8.0, but completely inactivated at 60°C for 15 rain. The enzyme had high lytic activity toward the cell walls of the various fungi tested except for the Zygomycetes group. This enzyme could be useful for protoplasting of filamentous fungi.
Ethanol Fermentatlon System Combined with Membrane Separator o f Pervaporation. KANJI ]V[_ATSUMOTO,TOSHIHIRO SUGIYAMA,and HARtrmKO Om~'A (Department of Material Science and Chemical
Engineering, Faculty of Engineering, YokohamaNational University, Tokiwadai, Hodogaya-ku, Yokohama 240, Japan) Hakkokogaku 64: 487492. 1986.
The characteristics of continuous ethanol fermentation system with membrane separator of pervaporatlon were investigated. The membrane used is of hydrophobic microporous hollow fibers of polypropylene. An ethanol-water mixture of high ethanol concentration was recovered continuously and directly from the fermentation broth. Intermittent backflnshing by compressed nitrogen gas prevented degradation of the permeation rate. The selectivity Production of Fungal Cell Wall Lytlc E n z y m e s of the membrane for ethanol was a little bit smaller by Oerskovia sp. CK and Some Propertics o f than that of distillation at normal pressure. The the Crude E n z y m e s . KATSVYA GOMX, DAUmO permeation rate of ethanol was almost llnearly proHOSAKA, NAOTO OKAZAKI*, TOSHIO TANAKA*, portional to the ethanol concentration of feed broth. CHIEKO KU/~AGAI, YUZURU IIMURA, and SHODO The carbon dioxide gas generated by fermentation HARA (National ResearchInstitute of Brewing, 2-6-30, promoted the permeation rate. The ethanol conTakinogawa, Kita-ku, Tokyo 114; *Office of Technical centration of permeation was estimated by the Officers, Kanto Shin-etsu Regional Taxation Bureau, fermentation dynamic equations.
1-3-2, Ohtemachi, Chooda-ku, Tokyo 100, Japan) Hakkokogaku 64: 479-485. 1986. Oerskovia sp. CK was cultivated with the mycelia of koji mold (Aspergillus oryzae) and produced fungal cell wall lytic enzymes inducibly. When chitin and dry yeast were used as substrates instead of fungal mycetia, the productivity of the lyric enzyme increased greatly. When the culture conditions for enzyme production were investigated, the maximum lytic activity was found at 30°C for 40 h in a medium containing 1% chitin powder and 0.5% dry yeast (pH 8.0). It was proved that chitlnasc was the main
Stimulation of filamentous Bulking o f Activated Sludge by Lactic Acid, Acetic Acid, and Glucose. RIKIYA TAKAHASHX, AKIHIKO SUZUKI, MASAHARU Suz~x, and HITOSHI YONEYAMA (Department of
Brewing and Fermentation, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156, Japan) Hakkokogaku 64: 493--498. 1986. An artificial sewage which contained small amounts of organic acids, such as acetic acid or lactic acid stimulated filamentous bulking of normal sewage activated sludge as well as glucose-containing artificial
577
Abstracts of the Articles Printed in Hakkokogaku Kaishi Vol. 64, No. 6 (1986)
Cultural Condition for Cell Mass Production of Aureobasidium pullulans f r o m Solvolysis pulping Waste Liquor. JUN HOSOKAWA, KAZUTOSHI Y0SHIHARA, M.A~HI NXSHPgA~tn, and HIROSHI K A m S H ~ (Government Industrial Research Institute
Sikoku, 2--3-3 Hananomiya-cho, Takamatsu-shi 761, Japan) Hakkokogaku 64: 473-477. 1986. The optimum cultural conditions were investigated for the cell mass production of strain A. pullulans IFO 6353 which has found to be the most promising organism that uses xylan in the solvolysis pulping waste liquor. The optimum cultural conditions were 29°C, initial pH 6.0-6.5. Ammonium sulfate and Lasparagine were suitable as supplementary nitrogen sources. The optimum concentrations of supplemental nutrients were 0.5% ammonium sulfate, 0.5-1.0% potassium dihydrogenphosphate, and 0. 1% magnesium sulfate. Further, the addition of yeast extract greatly increased the cell mass production. The cell mass reached 7.5 g per l of medium after 3 days under the optimum conditions (yeast extract, 0.25%). After 3 days of cultivation, A. pullulans IFO 6353 had used about 70% of the mono-, oligo-, and poly-saccharides in the waste liquor as one of the carbon sources.
contributor to the lytic activity on ftmgal cell walls. Optimum temperature and pH of the lytic activity were 45°C and 6.0-7.0, respectively. The lytie activity was stable at pH 5.0-8.0, but completely inactivated at 60°C for 15 rain. The enzyme had high lytic activity toward the cell walls of the various fungi tested except for the Zygomycetes group. This enzyme could be useful for protoplasting of filamentous fungi.
Ethanol Fermentatlon System Combined with Membrane Separator o f Pervaporation. KANJI ]V[_ATSUMOTO,TOSHIHIRO SUGIYAMA,and HARtrmKO Om~'A (Department of Material Science and Chemical
Engineering, Faculty of Engineering, YokohamaNational University, Tokiwadai, Hodogaya-ku, Yokohama 240, Japan) Hakkokogaku 64: 487492. 1986.
The characteristics of continuous ethanol fermentation system with membrane separator of pervaporatlon were investigated. The membrane used is of hydrophobic microporous hollow fibers of polypropylene. An ethanol-water mixture of high ethanol concentration was recovered continuously and directly from the fermentation broth. Intermittent backflnshing by compressed nitrogen gas prevented degradation of the permeation rate. The selectivity Production of Fungal Cell Wall Lytlc E n z y m e s of the membrane for ethanol was a little bit smaller by Oerskovia sp. CK and Some Propertics o f than that of distillation at normal pressure. The the Crude E n z y m e s . KATSVYA GOMX, DAUmO permeation rate of ethanol was almost llnearly proHOSAKA, NAOTO OKAZAKI*, TOSHIO TANAKA*, portional to the ethanol concentration of feed broth. CHIEKO KU/~AGAI, YUZURU IIMURA, and SHODO The carbon dioxide gas generated by fermentation HARA (National ResearchInstitute of Brewing, 2-6-30, promoted the permeation rate. The ethanol conTakinogawa, Kita-ku, Tokyo 114; *Office of Technical centration of permeation was estimated by the Officers, Kanto Shin-etsu Regional Taxation Bureau, fermentation dynamic equations.
1-3-2, Ohtemachi, Chooda-ku, Tokyo 100, Japan) Hakkokogaku 64: 479-485. 1986. Oerskovia sp. CK was cultivated with the mycelia of koji mold (Aspergillus oryzae) and produced fungal cell wall lytic enzymes inducibly. When chitin and dry yeast were used as substrates instead of fungal mycetia, the productivity of the lyric enzyme increased greatly. When the culture conditions for enzyme production were investigated, the maximum lytic activity was found at 30°C for 40 h in a medium containing 1% chitin powder and 0.5% dry yeast (pH 8.0). It was proved that chitlnasc was the main
Stimulation of filamentous Bulking o f Activated Sludge by Lactic Acid, Acetic Acid, and Glucose. RIKIYA TAKAHASHX, AKIHIKO SUZUKI, MASAHARU Suz~x, and HITOSHI YONEYAMA (Department of
Brewing and Fermentation, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156, Japan) Hakkokogaku 64: 493--498. 1986. An artificial sewage which contained small amounts of organic acids, such as acetic acid or lactic acid stimulated filamentous bulking of normal sewage activated sludge as well as glucose-containing artificial