Evaluation of fragment 1+2 in patients on oral anticoagulant therapy

ABSTRACTS

s22

OF 12TH NATIONAL CONGRESS

Vol. 70, Suppl. 1

C 028b DETERHINATItM

OF HYDROLYSIS

IN THROWIN-FIBRINOGEN Bizzi

& R.

Cattolica

Landolfi.

S. Cuore,

Application

of

controlled

reactions

interaction.

hydrolysis

of

allowed

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the

light

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effects A is

reaction

mechanism

to compute

is

is

to

the

binding

determine

interaction.

The pK value active

thrombin-fibrinogen

constant

constant. that

of

thrombin

rate

site. reaction

of

the

of

ionizable

B.

Universite

one of

these

measurement

of

groups of

is

the

9,

dependent

on solution

limiting

step

in a broad constant

in

the

pH interval

of

binding

k2 is the acylation encounter

KS as a function control

compatible equilibrium

opens the way to a rigorous

on diffusion-

thrombin-fibrinogen

of

relation:

thrombin-fibrinogen

groups

of

rate

equilibrium

The measurement

three

The

COWSTANT

M. Picozzi,

viscosity

linearly the

according to the following K app = Ks +qk2/k1 Km at a:y solution viscosity,

forward

of

was observed

to human a-thrombin,

k,

BINDING

dell’Emostasi,

on some aspects

fibrinopeptide

10 and allowed

OF EDUILIBRIUM

R. De Cristofaro,

00168 Roma, Italy. concerning

acylation

A.

OF PH.

Fisiopatologia

shed

of

the

fibrinopeptide

equilibrium

allowed

to

release

where Kmapp is the apparent constant,

1

aspects

means that

from 6 to

AND MEASUREMENT EFFECT

Ricerche

Largo F. Vito

The Km of This

ranging

Centro

theoretical

viscosity.

fibrinogen

MECHANISM

INTERACTION:

study

of

rate

and KS is pH (6-10)

thrombin-fibrinogen with

the

binding of this

histidine

57

constant

of

interaction.

C 029

EVALU/$TIGN OF FRAGMENT I +2 IN PATIENTSGN ORAL ANTICOAGULANT THERAPY Russo U., Arrigoni L., Banfi D., Gallo L., Poggio M., Rossi E. . Servizio Trasfljsionale, Laboratorio di Ematologia, Ospedale L.Sacco - Milano. Prothrombin fragment I+2 ( F 1+2 > is a marker of the activation of coagulation cascade and therefore it rapresents an index of thrombin formation. Oral anticoagulant therapy (OAT) reduces prothrombin syntesis as it is witnessed by the PT proiungation. We?es?ed the hypothesis that the reduction in prothrombin syntesis during OAT is associated to a reduction of its cleavage products. We studied a serie of 88 consecutive patients on OAT with acenocumarol and warfarin, monitored by Throm botest. They were affec?ed by different illnesses such as DVT, prosthetic heart valve or dilated cardiomyopathy, and they were on stable OAT for at least three months. 28 blorJd donors were considered 8s control group. Patients showed an INR ranging from 2.5 to 5.0 and a F 1 + 2 plasma level ( 0.4!+ 0.24) significantly lower than that of controls showing the efficacy of OAT.