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Evaluation of necrotizing effect of snake venoms
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Abstracts EVALUATION OF NECROTIZING EFFECT OF SNAKE VENOMS Eva Téboraké and Franti3ek Kornalik Eva Téboraké Frartiâek Kornalik
Institute of Pathophyaiology Charles University, 128 53 Praha 2, U nemocnice 5 Czechoslovakia
Haemorrhage and local necrosis are common consequences of bites eapecielly caused by Crotalidae or Viperidae snakes . No reliable and easily reproducible teat method for quantitative evaluation of the necrotic effect of venoma has been described . An attempt to introduce such e method is described . Various concentrations of venoma from different snakes were injected in 0.1 ml saline solution strictly intradermally in the back side of lightly anesthetized mice or rats . Groups of 3 - 5 animals were sacrificed 24 hours and five days respectively after the injection. Results are read on the inner surface of unstretched akin . Haemorrhagic activity is assessed in the former, necrotic in the later interval . Minimal haemorrhagic dose (MHD) is the amount of venom producing a haemorrhage of 10 mm in diameter (or widest distance in irregularly shaped haemorrhages) . Lesions of 5 mm in diameter are considered as minimal necrotizing dose (MND) . Doses of venoma differing by 20 - 30 ,~ug per 0 .1 ml can be reliably distinguished . Four main types of local lesions can be differentiated : 1 - Haemorrhage in 24 hours, resorbed within 5 days without subsequent necrosis . 2 - Haemorrhage wider than MHD resorbed within 2 - 5 days with central necrosis . 3 - Necrosis with minimal or no haemorrhage iii the first day . 4 - Coagulation necrosis developing within 24 hours in the whole haemorrhagic area . The variability in the development and morphology of the lesions indicates that different mechanisms are involved, very likely due to the specific qualities of enzymes in the venoma .
1 - Hommes, M., Tu, T .A . : Morphology of local tissue damage in experimental snake enveromat~on . B.J .exp .Path.52,538,1971 2 - Ohsaka, A. : Haemorrhagic, necrotizing and edema forming effects of snake venoma . In : Snake Venoma . Ed . Ch . Y. Lee, Springer Verlag N. Y. 1979, p .480
Abstracts EVALUATION OF NECROTIZING EFFECT OF SNAKE VENOMS Eva Téboraké and Franti3ek Kornalik Eva Téboraké Frartiâek Kornalik
Institute of Pathophyaiology Charles University, 128 53 Praha 2, U nemocnice 5 Czechoslovakia
Haemorrhage and local necrosis are common consequences of bites eapecielly caused by Crotalidae or Viperidae snakes . No reliable and easily reproducible teat method for quantitative evaluation of the necrotic effect of venoma has been described . An attempt to introduce such e method is described . Various concentrations of venoma from different snakes were injected in 0.1 ml saline solution strictly intradermally in the back side of lightly anesthetized mice or rats . Groups of 3 - 5 animals were sacrificed 24 hours and five days respectively after the injection. Results are read on the inner surface of unstretched akin . Haemorrhagic activity is assessed in the former, necrotic in the later interval . Minimal haemorrhagic dose (MHD) is the amount of venom producing a haemorrhage of 10 mm in diameter (or widest distance in irregularly shaped haemorrhages) . Lesions of 5 mm in diameter are considered as minimal necrotizing dose (MND) . Doses of venoma differing by 20 - 30 ,~ug per 0 .1 ml can be reliably distinguished . Four main types of local lesions can be differentiated : 1 - Haemorrhage in 24 hours, resorbed within 5 days without subsequent necrosis . 2 - Haemorrhage wider than MHD resorbed within 2 - 5 days with central necrosis . 3 - Necrosis with minimal or no haemorrhage iii the first day . 4 - Coagulation necrosis developing within 24 hours in the whole haemorrhagic area . The variability in the development and morphology of the lesions indicates that different mechanisms are involved, very likely due to the specific qualities of enzymes in the venoma .
1 - Hommes, M., Tu, T .A . : Morphology of local tissue damage in experimental snake enveromat~on . B.J .exp .Path.52,538,1971 2 - Ohsaka, A. : Haemorrhagic, necrotizing and edema forming effects of snake venoma . In : Snake Venoma . Ed . Ch . Y. Lee, Springer Verlag N. Y. 1979, p .480