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Evidence for the porcine pancreas containing and secreting a peptide related to glicentin (porcine gut GLI-1)
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EVIDENCE FOR THE PORCINE PANCREAS CONTAINING AND SECRETING A PEPTIDE RELATED TO GLICENTIN (PORCINE GUT GLI-I). A.J. Moody, J.J. Holst, L. Thim and S.Lindk~r Jensen, Novo Research Institute, Bagsvaerd; Dept. of Physiology C, University of Copenhagen, and Dept. of Surgical Gastroenterology, Rigshospitalet, Copenhagen, Denmark. Immunoreactants related to porcine glicentin have been shown by immunocytochemical means to be located in the A-cells of the pancreas of several animal species, wherein they are topologically segregated from glucagon. Significant amounts of glicentin-related peptides (GRP) have so far only been detected by RIA in extracts of porcine pancreas. This porcine GRP was partially purified, in order to compare its characteristics with those of glucagon and glicentin, and its secretion by the perfused porcine pancreas measured. Equimolar amounts of GRP and glucagon are found in extracts of porcine pancreas. The GRP is heterogeneous, as judged by gel permeation chromatography, but the bulk of the GRP had an apparent size between that of glicentin and that of glucagon. This material was devoid of GLI, and had an isoelectric point of 4.0. GRP was secreted by the perfused pancreas in the same molar concentrations as glucagon, and the secretion of GRP and of glucagon was increased to the same extent by i0 mmol/l arginine. GRP secreted by the perfused pancreas has the same pI and size as the bulk of the GRP extracted from the pancreas. It is concluded that these findings support the proposal that porcine glicentin is a partial homologue of proglucagon, and suggest that the pancreatic GRP described here is a fragment of proglucagon.
PLASMA GIP AND MOTIL!N RESPONSES TO ORAL LACTOSE IN HUMAN SUBJECTS WITH LACTOSE INTOLERANCE Morgan, L., Lord, C., Wong, J. and Marks, V., Department of Biochemistry, University of Surrey, Guildford, U.K. Eight healthy volunteers and 5 volunteers with lactose intolerance were given 5Og and lOOg oral lactose loads. Ingestion of lactose caused abdominal cramping and diarrhoea within 60 min in all subjects with lactose intolerance. Lactose tolerant subjects were symptomless. Plasma GIP and glucose levels rose significantly (p
EVIDENCE FOR THE PORCINE PANCREAS CONTAINING AND SECRETING A PEPTIDE RELATED TO GLICENTIN (PORCINE GUT GLI-I). A.J. Moody, J.J. Holst, L. Thim and S.Lindk~r Jensen, Novo Research Institute, Bagsvaerd; Dept. of Physiology C, University of Copenhagen, and Dept. of Surgical Gastroenterology, Rigshospitalet, Copenhagen, Denmark. Immunoreactants related to porcine glicentin have been shown by immunocytochemical means to be located in the A-cells of the pancreas of several animal species, wherein they are topologically segregated from glucagon. Significant amounts of glicentin-related peptides (GRP) have so far only been detected by RIA in extracts of porcine pancreas. This porcine GRP was partially purified, in order to compare its characteristics with those of glucagon and glicentin, and its secretion by the perfused porcine pancreas measured. Equimolar amounts of GRP and glucagon are found in extracts of porcine pancreas. The GRP is heterogeneous, as judged by gel permeation chromatography, but the bulk of the GRP had an apparent size between that of glicentin and that of glucagon. This material was devoid of GLI, and had an isoelectric point of 4.0. GRP was secreted by the perfused pancreas in the same molar concentrations as glucagon, and the secretion of GRP and of glucagon was increased to the same extent by i0 mmol/l arginine. GRP secreted by the perfused pancreas has the same pI and size as the bulk of the GRP extracted from the pancreas. It is concluded that these findings support the proposal that porcine glicentin is a partial homologue of proglucagon, and suggest that the pancreatic GRP described here is a fragment of proglucagon.
PLASMA GIP AND MOTIL!N RESPONSES TO ORAL LACTOSE IN HUMAN SUBJECTS WITH LACTOSE INTOLERANCE Morgan, L., Lord, C., Wong, J. and Marks, V., Department of Biochemistry, University of Surrey, Guildford, U.K. Eight healthy volunteers and 5 volunteers with lactose intolerance were given 5Og and lOOg oral lactose loads. Ingestion of lactose caused abdominal cramping and diarrhoea within 60 min in all subjects with lactose intolerance. Lactose tolerant subjects were symptomless. Plasma GIP and glucose levels rose significantly (p