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Experiences with the pregnancy test of Kapeller-Adler histidinuria in pregnant women
Department
of Reviews and Abstracts CONDUCTED
BY HUGO
EHRENFEST,
M.D.
Selected Abstracts Physiology Bowman,
Donald
Diagnosis
B.:
The
of Pregnancy,
of Pregnancy
Use of a Reducing J. Lab. 6; Clin. Med.
Factor of Pregnancy 24:
1072,
Urine in the
1939.
TO obviate time and expense, a chemical test for pregnancy would be of distinct value. Urinary pregnancy hormones are known to contain a reducing factor, and this substance might be developed for use in such a chemical test. The technique of the test is as follows: 12 c.c. of a fresh specimen of urine, specific gravity 1.020, are brought to a pH of 7.4 with 10 per cent sodium hydroxide; 9 O.C. of acetone are added and the precipitate centrifuged. The supernatant liquid is adjusted to a pH of 5.9 with 66 per cent sulfosalicylic acid. If a precipitate is formed, it is discarded; 1 C.C. of Xiirenson’s phosphate buffer is added to the liquid plus 18 C.C. of acetone and stirred and centrifuged for ten minutes. The supernatant fluid is withdrawn and the viscous precipitated layer contains the pregnancy reducing and gonadotropic factors. These are dissolved in 5 C.C. of water and 1 C.C. is treated with a few drops of 5 per cent starch solution and heated in the water bath at 38” C. until starch iodine color remains. In a total of 303 cases there were 108 correct positives and 80 correct negatives. The percentage of accuracy is 98.4 per cent. It is believed that the use of this reducing factor is a valid basis for a chemical test for pregnancy. W.
Kraus, Army, and Koenigstein, of Kapeller-Adler Histidinuria The positive histidine test sidered a very important sign of urine give the best results.
SERBIN.
Rudolf: Experiences With the Pregnancy Test in Pregnant Women, Chinese M. J. 59: 129, 1941. during pregnancy, for the diagnosis
though not of pregnancy.
specific, must be conMorning specimens C.
Efl’kemann, G., and Werle, IS.: Significance of the Blood in Pregnancy in Initiating 221,
B.
of Diminished Abortion,
0.
Histaminase
Zentralbl.
f.
MALAND.
Activity GynLk.
64:
1940.
The authors report an increase in the histamine-storing ability of the blood to 10 times normal by the sixth to eighth month of pregnancy as a result of increase in histaminase. A large amount of histaminase is found in the maternal blood The significance of the release of and circulating through the placental tissues. extra histamine into the circulation lies in its effect on tissue permeability. Outside of the inactivating effect of histaminase in pregnancy other substances prevent the full contraction-stimulating effect of histamine on the uterus. In cases of abortion, the authors found the maternal blood and serum were able to take up much more histamine than in normal pregnancy, regardless of the type of abortion, indicating a much lower blood-histamine content in this condition. In cases of habitual abortion, the authors believe, there is a constitutional adrenal 353
of Reviews and Abstracts CONDUCTED
BY HUGO
EHRENFEST,
M.D.
Selected Abstracts Physiology Bowman,
Donald
Diagnosis
B.:
The
of Pregnancy,
of Pregnancy
Use of a Reducing J. Lab. 6; Clin. Med.
Factor of Pregnancy 24:
1072,
Urine in the
1939.
TO obviate time and expense, a chemical test for pregnancy would be of distinct value. Urinary pregnancy hormones are known to contain a reducing factor, and this substance might be developed for use in such a chemical test. The technique of the test is as follows: 12 c.c. of a fresh specimen of urine, specific gravity 1.020, are brought to a pH of 7.4 with 10 per cent sodium hydroxide; 9 O.C. of acetone are added and the precipitate centrifuged. The supernatant liquid is adjusted to a pH of 5.9 with 66 per cent sulfosalicylic acid. If a precipitate is formed, it is discarded; 1 C.C. of Xiirenson’s phosphate buffer is added to the liquid plus 18 C.C. of acetone and stirred and centrifuged for ten minutes. The supernatant fluid is withdrawn and the viscous precipitated layer contains the pregnancy reducing and gonadotropic factors. These are dissolved in 5 C.C. of water and 1 C.C. is treated with a few drops of 5 per cent starch solution and heated in the water bath at 38” C. until starch iodine color remains. In a total of 303 cases there were 108 correct positives and 80 correct negatives. The percentage of accuracy is 98.4 per cent. It is believed that the use of this reducing factor is a valid basis for a chemical test for pregnancy. W.
Kraus, Army, and Koenigstein, of Kapeller-Adler Histidinuria The positive histidine test sidered a very important sign of urine give the best results.
SERBIN.
Rudolf: Experiences With the Pregnancy Test in Pregnant Women, Chinese M. J. 59: 129, 1941. during pregnancy, for the diagnosis
though not of pregnancy.
specific, must be conMorning specimens C.
Efl’kemann, G., and Werle, IS.: Significance of the Blood in Pregnancy in Initiating 221,
B.
of Diminished Abortion,
0.
Histaminase
Zentralbl.
f.
MALAND.
Activity GynLk.
64:
1940.
The authors report an increase in the histamine-storing ability of the blood to 10 times normal by the sixth to eighth month of pregnancy as a result of increase in histaminase. A large amount of histaminase is found in the maternal blood The significance of the release of and circulating through the placental tissues. extra histamine into the circulation lies in its effect on tissue permeability. Outside of the inactivating effect of histaminase in pregnancy other substances prevent the full contraction-stimulating effect of histamine on the uterus. In cases of abortion, the authors found the maternal blood and serum were able to take up much more histamine than in normal pregnancy, regardless of the type of abortion, indicating a much lower blood-histamine content in this condition. In cases of habitual abortion, the authors believe, there is a constitutional adrenal 353