- Email: [email protected]
Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets
Journal Pre-proof Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets Liliana Anjos, Patricia I.S. Pinto, Theofania Tsironi, George Dimopoulos, Soraia Santos, Cátia Santa, Bruno Manadas, Adelino Canario, Petros Taoukis, Deborah M. Power PII:
S2352-3409(19)30806-6
DOI:
https://doi.org/10.1016/j.dib.2019.104451
Reference:
DIB 104451
To appear in:
Data in Brief
Received Date: 17 May 2019 Revised Date:
7 June 2019
Accepted Date: 21 August 2019
Please cite this article as: L. Anjos, P.I.S. Pinto, T. Tsironi, G. Dimopoulos, S. Santos, C. Santa, B. Manadas, A. Canario, P. Taoukis, D.M. Power, Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets, Data in Brief, https://doi.org/10.1016/j.dib.2019.104451. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Published by Elsevier Inc.
MetaMeta-Data
*Title:
Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus (Dicentrarchus labrax) labrax) fillets
*Authors:
Liliana Anjos1,*, Patricia I. S. Pinto1,*, Theofania Tsironi2, George Dimopoulos2, Soraia Santos1, Cátia Santa3,4, Bruno Manadas3, Adelino Canario1, Petros Taoukis2,#, Deborah M. Power1,#
*Affiliations:
1
Comparative Endocrinology and Integrative Biology
Group (CEIB), Centro de Ciencias do Mar (CCMAR), University of Algarve, Faro, Portugal 2
Laboratory of Food Chemistry and Technology,
School of Chemical Engineering, National Technical University of Athens (NTUA), Greece 3
Center for Neuroscience and Cell Biology (CNC),
University of Coimbra, Portugal 4
Institute for Interdisciplinary Research, University
of Coimbra (IIIUC) * Equal contribution #-corresponding authors *Contact email:
Deborah M. Power, [email protected]; Petros Taoukis, [email protected]
*Co CoCo-authors: authors
Liliana Anjos, [email protected]; Patricia I. S. Pinto, [email protected]; Theofania Tsironi, [email protected]; George Dimopoulos, [email protected]; Soraia Santos, [email protected]; Cátia Santa, [email protected]; Bruno Manadas, [email protected]; Adelino Canario, [email protected].
*CATEGORY:
Biochemistry; Food science; Molecular Biology
Data Article
Title: Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus (Dicentrarchus labrax) labrax) fillets Authors: Liliana Anjos1,*, Patricia I. S. Pinto1,*, Theofania Tsironi2, George Dimopoulos2, Soraia Santos1, Cátia Santa3,4, Bruno Manadas3, Adelino Canario1, Petros Taoukis2,#, Deborah M. Power1,# Affiliations: 1 Comparative Endocrinology and Integrative Biology Group (CEIB), Centro de Ciencias do Mar (CCMAR), University of Algarve, Faro, Portugal 2
Laboratory of Food Chemistry and Technology, School of Chemical Engineering,
National Technical University of Athens (NTUA), Greece 3
Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Portugal
4
Institute for Interdisciplinary Research, University of Coimbra (IIIUC)
* These authors contributed equally for this work #Corresponding authors. Postal address: Deborah Power, Centro de Ciencias do Mar (CCMAR), University of Algarve, Campus de Gambelas, Faro, Portugal; Tel: +351800100; E-mail: [email protected] Petros Taoukis, Iroon Polytechniou, Zografou 15780, Athens, Greece; Tel: +302107723171; E-mail: [email protected] Short title: Data from high pressure processing and quality evaluation of fish fillets Contact email: email [email protected], [email protected] Abstract Fresh fish are highly perishable food products and their short shelf-life limits their commercial exploitation and leads to waste, which has a negative impact on aquaculture sustainability. New non-thermal food processing methods, such as high pressure (HP) processing, prolong shelf-life while assuring high food quality. The effect of HP processing (600MPa, 25ºC, 5min) on European sea bass (Dicentrarchus labrax) fillet quality and shelf life was investigated. The data presented comprises microbiome and proteome profiles of control and HP-processed sea bass fillets from 1 to 67 days of isothermal storage at 2°C. Bacterial diversity was analysed by Illumina high-throughput sequencing of the 16S rRNA gene in pooled DNAs from control or HP-processed fillets after 1, 11 or 67 days and the raw reads were deposited in the NCBI-SRA database with
accession number PRJNA517618. Yeast and fungi diversity were analysed by highthroughput sequencing of the internal transcribed spacer (ITS) region for control and HP-processed fillets at the end of storage (11 or 67 days, respectively) and have the SRA accession number PRJNA517779. Quantitative label-free proteomics profiles were analysed by SWATH-MS (Sequential Windowed data independent Acquisition of the Total High-resolution-Mass Spectra) in myofibrillar or sarcoplasmic enriched protein extracts pooled for control or HP-processed filets after 1, 11 and 67 days of storage. Proteome data was deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD012737. These data support the findings reported in the associated manuscript “High pressure processing of European sea bass (Dicentrarchus labrax) fillets and tools for flesh quality and shelf life monitoring”,
Tsironi
et
al.
2019,
JFE
262:83-91,
doi.org/10.1016/j.jfoodeng.2019.05.010.
Keywords: Colour and texture; European sea bass; fish fillet; high pressure; 16S rRNA microbiome; quantitative proteomics
Specifications Table Subject area
Food science
More specific subject
Food processing, Aquaculture
area Type of data
Tables, figures, supplementary tables
How data was
CR-Minolta chromameter, Stable Micro Systems TA-XT2i texture
acquired
analyzer, Illumina MiSeq, ABSciex Triple TOF 5600 LC-SWATHMS system with information-dependent acquisition (IDA)
Data format
Raw, metadata
Experimental factors
Marine cultured sea bass were refrigerated on ice, industrially scaled, the head removed, filleted and submitted to high pressure or not (control) and then isothermally stored (2ºC) for different periods.
Experimental
Colour and texture measurements; DNA extraction, quality
features
evaluation, microbiome library construction of 16S rRNA and internal transcribed spacer PCR amplified regions, sequencing and bioinformatics analysis; protein extractions, quality and quantity estimation, LC-SWATH-MS identification and quantification.
Data source location
Koropi, Attica, Greece (37°53'12.4"N; 23°52'40.4"E)
Data accessibility
Data is available in this article and at https://www.ncbi.nlm.nih.gov/sra/ (projects PRJNA517618 and PRJNA517779) and at the ProteomeXchange Consortium via the PRIDE repository with identifier PXD012737.
Related research
Theofania Tsironi, Liliana Anjos, Patricia I. S. Pinto, George
article
Dimopoulos, Soraia Santos, Cátia Santa, Bruno Manadas, Adelino Canario, Petros Taoukis, Deborah Power “High pressure processing of European sea bass (Dicentrarchus labrax) fillets and tools for flesh quality and shelf life monitoring”. Journal of Food Engineering 262, 83 – 91 https://doi.org/10.1016/j.jfoodeng.2019.05.010
Value of the Data •
The data represents an integrated view of the impact of novel non-thermal processing technologies on fish fillet quality and safety and makes a relevant contribution to the food loss and waste paradigm.
•
A metagenomics approach for the microbiome used 16s rRNA (bacteria) and internal transcribed spacer (for yeast and fungi) sequencing to provide a global and in-depth overview of microorganism diversity and dynamics in control and HP processed fillets during storage at 2ºC.
•
SWATH-MS proteomics is used to give insight for the first time into the global impact of HP processing and cold storage on the proteins in sea bass fillets.
•
The study demonstrates how integration of conventional experimental methods and novel omics technologies corroborate and substantially extend the outcome of previous work.
•
HP processing is revealed as an emerging processing approach that prolongs the shelf life of highly perishable commodities such as fish fillets.
Data Table 1 summarizes 16S rRNA sequencing quality statistics of HP-treated and control fillets at different time-points during storage, and Figure 1 presents the alpha rarefaction analysis of the same data, showing that all sequencing libraries were near to saturation. Table 2 lists the main bacterial genera detected and their relative abundance in each sequencing library. Table 3 lists all bacterial genera detected including those present in low abundance (<1% of the total genera detected). Similarly, Tables 4 and 5 contain, respectively, lists of the most abundant bacteria species and complete list of detected bacteria, together with the Shannon diversity index and CHAO1 Richness Estimation for each library. Table 6 summarizes the ITS sequence quality statistics for yeast and fungi of HP-treated and control fillets at the end of storage and Figure 2 presents the alpha rarefaction analysis of the same data, showing that all sequencing libraries were near to saturation. Tables 7 and 9 list, respectively, the main fungal genera and species detected by ITS in 1% or more of the reads. Tables 8 and 10 list, respectively, the full list of fungal genera and species detected by ITS. Table 11 summarizes the number of proteins and peptides from myofibrillar and sarcoplasmicenriched protein extracts that were identified or quantified by SWATH analysis in HPprocessed or control fillets at different storage times. Tables 12 and 13 (spreadsheet format) list the quantification parameters of the total proteome profiles of myofibrillar and sarcoplasmic extracts, respectively. Table 14 lists the 38 proteins from the myofibrillar extract and Table 15 lists the 263 proteins from the sarcoplasmic extract (in spreadsheet format) that changed greater than 2-fold between the control and HPprocessed fillets and/or between cold storage times; the relative proportions of the proteins are summarized in Figure 3. Experimental Design, Materials, and Methods Experimental setset-up and fish fillet samples European sea bass (Dicentrarchus labrax) (weight: 110 ± 10 g) from Nireus Aquaculture, were stunned on ice slush (0°C) immediately upon harvesting, size sorted and transported to the filleting line within 1 day. In the industrial facilities the fish was filleted after descaling and discarding of the head. Sea bass fillets were rinsed in tap
water, placed in polystyrene boxes with appropriate quantities of flaked ice (0°C) and transported to the Laboratory of Food Chemistry and Technology (NTUA, Athens, Greece) within 2-3 hours. A laboratory pilot scale Food Pressure Unit (FPU 1.01, Resato International BV, Roden, Holland) with a maximum operating pressure of 1000 MPa was used for high pressure treatments. The high-pressure unit had a 1.5 L volume and a multivessel system consisting of six vessels of 45 mL capacity each. All high-pressure vessels were surrounded by a circulating jacket connected to a heating-cooling system. The pressure transmitting fluid was polyglycol ISO viscosity class VG 15 (Resato International, BV, Roden, Holland). Fish fillets were packed in pouches (two per pouch) consisting of a multilayer (PP-PE) packaging material. HP processed fillets were vacuum-packed and treated in-pack at 600 MPa and 25°C for 5 min, according to [1]. The non-processed (control) samples were stored aerobically in non-sealed pouches, simulating conventional aerobic retail display facilities. Control and HP-treated (HP) fillets were stored at 2ºC under controlled isothermal conditions in high-precision (±0.2 °C) low-temperature incubators (Sanyo MIR 153, Sanyo Electric, Ora-Gun, Gunma, Japan), monitored with electronic, programmable miniature data-loggers (COX TRACER ®, Belmont, NC). Samples of ca 5 cm2 section were collected from fillets at different storage times: 1 day (C1 and HP1 groups), 11 days (C11 and HP11), 32 days (HP32) and 67 days (67HP). Samples from control fillets were not collected for the last two time points since they had deteriorated to unacceptable levels after 11 days of storage.
Colour and texture parameters Fillet colour change over time and treatment was measured using a CR-Minolta Chromameter (Minolta Co., Chuo-Ku, Osaka, Japan) with an 8mm diameter measuring area. The instrument was standardized under “C” illuminant conditions according to the CIE (Commission International de l’ Eclairage) using a standard white reference tile (calibration plate CR-200, L=97.50, a=-0.31, b=-3.83). Measurements of CIELab values (L-value: lightness, a-value: redness and greenness, b-value: yellowness and blueness) were made in three different points of the flesh on the upper side of the fillet (lateral line) and 3 fillets were tested per group and sampling point. Texture parameters were evaluated using a texture analyser with a load cell of 5 kg (TAXT2i, Stable Micro Systems, Godalming, Surrey, United Kingdom). A compression
aluminium plate of 75 mm or 20 mm diameter was selected. Double compression was applied to construct the texture profile analysis (TPA) parameters of 3 different fillets per group and sampling point. The aluminium plate approached the sample at the speed of 0.5 mm/sec and pressed 2 mm into the fish flesh. Then the force was reduced and the sample was allowed to rebound 5 s before the second compression. Force-time curves were obtained and texture parameters (hardness, cohesiveness and adhesiveness) were determined using Texture Expert Exceed Application (Version 2.64, Stable Micro Systems Ltd) [2].
DNA extracts Total DNA was separately extracted from 4 fillets from each of the groups C1, C11, HP1, HP11 and HP67, using a DNeasy Blood & Tissue Kit (Qiagen) with modifications. The excision of approx. 40 mg taken from the fillet surface was carried out under sterile conditions. Muscle samples were cut into small pieces, added to 2 ml sterile tubes with 200 µl of lysis buffer (20Mm Tris-HCl, pH 8; 2mM sodium EDTA; 1.2% Triton X-100; 40 mg/ml Lysozyme), 200 µl of AL buffer and approx. 400 mg of glass beads (0.1mm zirconia/silica beads, Biospec). The tubes were maintained on ice until mechanical disruption was carried out at room temperature in a Bertin Precellys 24 homogeniser; the disruption procedure was repeated in two pulses of 15 s at 6800 rpm. Tubes were incubated for 30 min at 37 ºC to complete the enzymatic lysis, and then 25 µl of proteinase K (supplied with the kit) was added to each tube, which were incubated for a further 30 min at 56 ºC. Tubes were centrifuged for 1 min at 8000 rpm and the lysed supernatant was recovered and incubated with RNAse A (10 µl of 10 mg/ml) for 10 min at room temperature. The remaining steps of the extraction method were performed as recommended in the protocol of the DNeasy Blood & Tissue Kit, with a final elution of extracted DNA in 50 µl of EB buffer (10Mm Tris-HCl, pH 8). DNA quality and integrity were analysed using a Nanodrop spectrophotometer and 1% agarose gel electrophoresis.
Microbiome library preparation, sequencing and bioinformatics analyses DNA pools were prepared from individual DNA extracts (ca. 250 ng each) and then concentrated with an RNeasy Micro Kit (Qiagen), following the manufacturer’s instructions, and eluted into 22 µl EB buffer. Five bacterial microbiome libraries (groups C1, C11, HP1, HP11 and HP67) were prepared from 75-210 ng of pooled DNA using the
16S Metagenomic Sequencing Library Preparation protocol (for the Illumina MiSeq system) and primers spanning the V3 and V4 regions of the 16S rRNA gene [3]. Two fungal microbiome libraries (C11 and HP67) were prepared from 30-130 ng of pooled DNA, using the same protocol as outlined above but with a set of primers directed against regions ITS3-ITS4 from Ascomycetes and Basidiomycetes [4]. Libraries were sequenced by Lifesequencing S.L.-ADM (Valencia, Spain) using an Illumina MiSeq instrument. Sequence data analysis, included: trimming of adaptors and filtering of lowquality reads that had a minimum value Q20 and minimum read length of 200 nucleotides; removal of chimeras and identification and classification of operational taxonomic units (OTU). Data analysis was carried out as described in [5]. For OTU assignments, sequence similarity searches were made against the NCBI 16S rRNA database (for the 16S libraries) or against an ITS built database of fungal ITS sequences extracted from NCBI (for the ITS libraries), with a cut-off set at 97% identity. Global microbiome data analyses were performed as described in [5], using a pipeline developed by Lifesequencing S.L.-ADM, and CD-HIT software [6] was used for hierarchy clustering.
Quantitative proteomics Differences in the protein content of control (C1, C11) and HP fillets (HP1, HP67) were analysed by SWATH-MS (Sequential Window data independent Acquisition of the Total High-resolution-Mass Spectra) using pools of myofibrillar or sarcoplasmic enriched protein extracts (n=6 per group). Myofibrillar and sarcoplasmic enriched protein extracts (based on [7] with modifications) were prepared from individual fillets (n=6 individuals per group). The extraction buffer used for the myofibrillar extracts was 50 mM Tris buffer pH 7.2, 100 mM DTT, 1.7% SDS and for sarcoplasmic extracts was 10 mM Tris buffer pH 7.2 and both buffers contained a protease inhibitor cocktail (Sigma, US). The muscle sample (0.5 g) was reduced to a powder in liquid nitrogen and ≈0.1 g was solubilized in extraction buffer (ratio: 1 g of tissue/8 ml of buffer), and mechanically disrupted using 0.5 mm zirconia/silica beads (Biospec) in a Bertin Precellys 24 homogeniser using 2 cycles of 20 s at 6,800 rpm. The homogenates were boiled at 95 ºC for 10 min and allowed to cool at room temperature. The soluble protein fraction was separated by centrifugation at 28,000 g for 15 min at 20 ºC, alkylated with 40 % acrylamide solution (1:15 of acrylamide: protein solution v/v) and stored at -80 ºC. The soluble protein concentration was determined using the Bradford assay with a bovine
serum albumin standard set (#500–0006 and #500–0207, BioRad, USA). The electrophoretic profile of the myofibrillar and sarcoplasmic enriched protein extracts was analysed by 1D SDS-PAGE (12%) according to [8] followed by Coomassie blue staining. For SWATH-MS proteomic analysis the protein extracts were gel digested using the short-GeLC method [9]. Briefly, a volume corresponding to 50 μg was prepared by pooling protein extracts from 6 individuals/treatment, and a further sample was prepared that consisted of 10 μg from each experimental group pool. The sample pools were fractionated by SDS- PAGE. Selected gel regions were excised and processed as in [10], for destaining, dehydration, rehydration, in-gel protein digestion using trypsin, peptide extraction and solid phase extraction with C18 sorbent (OMIX tip, Agilent Technologies). Samples were analysed using SWATH-MS on a Triple TOF™ 5600 System (Sciex®, Framingham, MA) using information-dependent acquisition (IDA) of the pooled mixture of all samples; followed by SWATH acquisition of each individual group pool. Peptide separation was performed using liquid chromatography (nanoLC Ultra 2D, Eksigent) on a ChromXP C18CL reverse phase column (300 µm × 15 cm, 3 µm, 120Å, Eksigent) at 5 µL/min with a 45 min gradient from 2% to 35% acetonitrile in 0.1% FA with 5% DMSO, and the peptides were eluted into the mass spectrometer using an electrospray ionization source (DuoSpray™ Source, Sciex). IDA experiments were performed by analysing each fraction of the pooled mixture. The mass spectrometer was set for IDA scanning full spectra (350-1250 m/z) for 250 ms, followed by up to 100 MS/MS scans (100–1500 m/z from a dynamic accumulation time – minimum 30 ms for precursors above the intensity threshold of 1000 counts per second (cps) – in order to maintain a cycle time of 3.3 s). Candidate ions with a charge state between +2 and +5 and counts above a minimum threshold of 10 cps were isolated for fragmentation and one MS/MS spectra was collected before adding those ions to the exclusion list for 25 s (mass spectrometer operated by Analyst® TF 1.7, Sciex). Rolling collision energy was used with a collision energy spread (CES) of 5. The SWATH setup was as in [11] with the same chromatographic conditions used for IDA acquisitions. Briefly, the mass spectrometer was operated in a looped product ion mode. The SWATH MS setup was designed specifically for the samples to be analysed, in order to adapt the SWATH windows to the complexity of this batch of samples. A set of 60 SWATH windows of variable width (containing 1 m/z for window overlap) was constructed covering the precursor mass range of 350-1250 m/z. A 200 ms survey scan
(350-1250 m/z) was acquired at the beginning of each cycle for instrument calibration and SWATH MS/MS spectra were collected from 100–1500 m/z for 50 ms resulting in a cycle time of 3.3 s from the precursors ranging from 350 to 1250 m/z. The collision energy for each window was determined according to the calculation for a charge +2 ion centered upon the window with variable CES (Collision Energy Spread) according to the window. Peptide identification and library generation were carried out using Protein Pilot software (v5.0, Sciex®) and the following search parameters: 1) comparison against the predicted proteins from the sea bass genome database (June 2012 draft assembly dicLab v1.0c with annotation from July 2013; file diclab1_pep.faa.gz downloaded from http://seabass.mpipz.mpg.de/DOWNLOADS/; [12]; 2) acrylamide alkylation; 3) trypsin digestion (ParagonTM Algorithm). The SWATHTM processing plug-in for PeakViewTM (v2.0.01, ABSciex®) was used for SWATH data processing, as described in [9] with minor modifications. Peptides were selected automatically from the library following the criteria: i) unique peptides for a specific targeted protein were ranked by the intensity of the precursor ion from the IDA analysis; ii) peptides that contained biological modifications and/or were shared between different protein entries/isoforms were excluded. Up to 15 peptides were chosen per protein, and SWATHTM quantification was attempted for all proteins considered as positive identifications. Peptide retention time was adjusted using the malE-GFP peptides. Up to 5 target fragment ions per peptide were automatically selected and the peak groups were scored following the criteria described in [13]. Protein levels were estimated by summing all the transitions from all the peptides for a given protein as described by [14] and normalized to the total intensity of the sample at the protein level. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [15] via the PRIDE [16] partner repository with the dataset identifier PXD012737. In proteomic data analysis, the count ratio method was used to compare the relative protein abundance modified by HP (HP1/C1; HP67/C11) or storage time (HP1/HP67; C1/C11) in each type of extract, considering 2-fold change as the threshold. Protein lists that changed with the experimental conditions were analysed using the proportional Venn diagram tool BioVenn (http://www.biovenn.nl) [17], UNIPROT (https://www.uniprot.org/) [18] and BRENDA (https://www.brenda-enzymes.org) [19] for screening of enzymes, structural proteins and other classifications. The zebrafish (Danio rerio) orthologues for the sea bass proteins were obtained using stand-alone
BlastX (with E value < 10-10) against the Ensembl zebrafish protein predictions (GRC Zebrafish
Build
10,
INSDC
Assembly
GCA_000002035.3
from
Sep
2014,
https://www.ensembl.org [20]) in order to establish a comparative annotation.
Acknowledgments This work was carried out under project “SUStainable production of HIgh quality aquaculture FISH using innovative tools and production strategies and integrating novel processing methods and cold chain management (Acronym: SUSHIFISH), co-funded by the COFASP ERA-NET partners (2nd call, 2016 - 2019), which received funding from the European Union’s seventh framework programme for research, technological development and demonstration under grant agreement no. 321553, together with Greek
national
funds
through
the
Operational
Program
"Competitiveness,
Entrepreneurship and Innovation 2014-2020" of the National Strategic Reference Framework (NSRF) and Portuguese national funds from FCT (Foundation of Science and Technology) through project COFASP/0002/2015. Support was also received from FCT through project UID/Multi/04326/2019, and from the operational programmes CRESC Algarve 2020 and COMPETE 2020 through project EMBRC.PT ALG-01-0145-FEDER022121, and through the European Marine Biological Research Infrastructure ClusterEMBRIC (EU H2020 research and innovation program, agreement nº 654008). The EMBRIC
configurator
data
management
service
(doi:10.7490/f1000research.
1116538.1) was used for advice on data coordination and standards. LA, PP and CS received
FCT
fellowships
SFRH/BPD/79105/2011,
SFRH/BPD/84033/2012
and
SFRH/BD/88419/2012, respectively. Authors contributions TT, PT and DMP conceived the study; AVMC, PT and DMP provided the resources; TT and GD performed the High-Pressure experiments, fish sampling and conventional microbiological, texture and colour analysis; Microbiome analysis of the fish fillets was performed by PISP and SS. LA, CS and BM carried out the protein analysis and SWATHproteomics and data analysis; TT, PT, DMP, AVMC, LA, PISP critically analysed and reviewed all the results of the study; TT, LA, PISP and DMP drafted the manuscript, which was critically reviewed and approved by all authors.
Figures and tables Figure legends Figure 11- Rarefaction plots for the microbiome libraries sequenced by 16S rRNA gene sequencing (bacterial diversity), determined based on all operational taxonomic units (OTUs) found at the genus level, including annotated and non-annotated sequences (no hits). Figure 22- Rarefaction plots for the microbiome libraries directed at the fungal ITS region, determined based on all operational taxonomic units (OTUs) found at the genus level, including annotated and non-annotated sequences (no hits). Figure 33- Venn diagrams representing the number of proteins that had a more than 2fold modified abundance in HPP sea bass fillets compared to the control groups (C) at the equivalent storage time in days (eg. short-term, C1 and HP1 or long-term, C11, HP67). Proteins were identified and quantified SWATH proteomics. For Myofibrillar (red) or sarcoplasmic (blue) protein extracts, the size of the circles is proportional to the number of proteins that had a modified expression (the number of proteins are indicated next to the circles). The proteins that changed in the same way between the different treatment groups at the beginning and end of storage are located in the intersection of two circles in the Venn diagram (the number of common proteins is underlined). Tables Table 11- Sequencing statistics for the 16S rRNA gene microbiome libraries sequenced in the study.
The number of Number of raw, paired end and filtered reads, average length (in base pairs, bp) and average quality (Phred Q value) are shown for each sea bass muscle microbiome library. Table 22- The main bacterial genera detected by 16s rRNA gene sequencing of control sea bass fillets or those subject to high-pressure (HP). The relative proportion of a given genera relative to the total reads identified in each library is shown as a percentage. Only genera detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion). Table 33- List of all the bacterial genera detected by 16S rRNA gene sequencing in sea bass fillets, including genera present at a low proportion (<1% of all the genera detected) per library. Values represent the relative proportion of each genus relative to the total reads identified in each library. All detected genera are listed but those at < 1% of the total microbiome in each library are shaded in grey. Table 44- The main bacterial species detected (>1% of all the species detected) by 16S rRNA gene sequencing. The relative proportion of a given species relative to the total reads identified in each library is shown as a percentage. Only species detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion). Table 55- All bacterial species detected by 16S rRNA gene sequencing in control or HPP sea bass fillets (including those present at <1% of the global microbiome). Values represent the relative proportion of each species relative to the total reads identified in each library. All detected species are listed but those at < 1% of the total microbiome in each library are shaded in grey.
Table 66- Sequencing statistics for the fungal ITS microbiome libraries prepared from control and HPP sea bass fillets at the end of storage. The number of Number of raw, paired end and filtered reads, average length (in base pairs, bp) and average quality (Phred Q value) are shown for each sea bass muscle microbiome library. Table 77- Main fungal genera detected by ITS sequencing, at >1% of all the genera detected per library. The relative proportion of a given genera relative to the total reads identified in each library is shown as a percentage. Only genera detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion). Table 88- All fungal genera detected by ITS sequencing, including genera represented at low proportions (<1% of all the genera detected) per library. Values represent the relative proportion of each genus relative to the total reads identified in each library. All detected genera are listed but those at < 1% of the total metagenome in each library are shaded in grey. Table 99- Main fungal species detected by ITS sequencing, at >1% of all the species detected per library. The relative proportion of a given species relative to the total reads identified in each library is shown as a percentage. Only species detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the metagenome they are shaded from grey (low proportion) to red (high proportion). Table 1010- All fungal species detected by ITS sequencing, including those represented at low proportions (<1% of all the species detected) per library. Values represent the relative proportion of each species relative to the total reads identified in each library. All detected species are listed but those at < 1% of the total fungal metagenome in each library are shaded in grey. Table 1111- Summary of the number of proteins and peptides identified or quantified in SWATH proteomic analysis.
Table 1212- Total proteome quantified by SWATH analysis for myofibrillar extracts. Table 1313- Total proteome quantified by SWATH analysis for sarcoplasmic extracts. Table 1414- Proteins identified by SWATH proteomics with a greater than a 2-fold change with HPP and/or storage time relative to the control in the myofibrillar extracts. Proteins whose levels decreased are shaded in green and whose levels increased in red. Table 1515- Proteins identified by SWATH proteomics with a greater than a 2-fold change with HPP and/or storage time in the sarcoplasmic extracts. Proteins whose levels decreased are shaded in green and whose levels increased in red.
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Table 1‐ Sequencing statistics for the 16S rRNA gene microbiome libraries sequenced in the study. The number of Number of raw, paired‐end and filtered reads, average length (in base pairs, bp) and average quality (Phred Q value) are shown for each sea bass muscle microbiome library.
Library C1 C11 HP1 HP11 HP67 Total Average
Raw reads Paired‐end reads Filtered reads Av. Length Total Mb Av. Qual. 65064 32116 29788 428 12.7 37.3 67298 33327 31625 431 13.6 37.3 53178 26176 23953 426 10.2 37.2 56300 27945 26364 429 11.3 37.2 55296 27443 26003 430 11.2 37.3 297136 147007 137733 59.0 59427 29401 27547 429 11.8 37.3
Table 10‐All fungal species detected by ITS sequencing, including those represented at low propo Values represent the relative proportion of each species relative to the total reads identified in ea All detected species are listed but those at < 1% of the total fungal metagenome in each library a Main species Saccharomyces cerevisiae Malassezia restricta Cordyceps confragosa Cluster 130 Candida hollandica Mortierella hyalina Aspergillus vitricola Rhodotorula sp. 1 CS‐2017 Peniophora cinerea Didymella arachidicola Malassezia globosa Saccharomyces sp. Candida zeylanoides Itersonilia perplexans Cladosporium sp. Phanerochaete chrysosporium Pichia fermentans Penicillium camemberti No Hit Schizophyllum commune Theobroma speciosum Antrodia sinuosa Debaryomyces hansenii Candida sp. CBS 10854 Cutaneotrichosporon curvatus Ramularia eucalypti Phlebia subserialis Parastagonospora nodorum Sistotremastrum guttuliferum Alternaria chlamydosporigena Meyerozyma guilliermondii Cluster 172 Platanus x hispanica Carex radiata Aspergillus pseudoglaucus Candida deformans Diplosphaera chodatii Aegopodium podagraria Fragaria virginiana Urtica dioica Rhodotorula glutinis Fusarium merismoides Galactomyces candidum
C11 96.749 0.443 0.316 0.223 0.165 0.156 0.136 0.127 0.116 0.098 0.096 0.093 0.090 0.084 0.076 0.075 0.073 0.061 0.055 0.055 0.046 0.038 0.038 0.035 0.032 0.032 0.032 0.032 0.026 0.026 0.023 0.023 0.020 0.020 0.017 0.017 0.017 0.014 0.014 0.014 0.012 0.012 0.012
HP67 0.332 3.186 8.923 0.000 2.038 0.000 2.778 0.000 0.000 0.000 0.796 0.000 2.058 0.000 7.151 0.000 0.585 0.000 0.325 0.000 0.000 0.367 0.000 0.000 0.393 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.339 0.000 0.125 0.000 0.000 0.000 0.000 0.000 0.256 0.104 0.000
Candida parapsilosis Candida sp. K2 Salvia coccinea Sporidiobolus metaroseus Juglans regia Stellaria media Lecania cyrtella Yarrowia lipolytica Penicillium olsonii Wallemia muriae Linum bienne Spinacia oleracea Pistacia lentiscus Diplotaxis tenuifolia Cannabis sativa Bacillus cytotoxicus Petroselinum crispum Triticum turgidum Salvia reuteriana fungal sp. suzu‐1 Saitozyma podzolica Trichoderma harzianum Cryptococcus sp. HB 1222 Pichia membranifaciens Lactobacillus letivazi Helotiales sp. EF830 Phanerochaete sp. JCM 28409 Penicillium robsamsonii Penicillium aeris Achillea wilsoniana Zygosaccharomyces lentus Candida sp. WSYC 780 Penicillium sp. NX‐deepsea Rhodotorula mucilaginosa Cluster 46 Verrucaria csernaensis Metschnikowia bicuspidata Urtica membranacea Sistotremastrum chilensis Cluster 77 Lophodermium sp. 3396 Cluster 52 Massarinaceae sp. 113 OG‐2012 Rhizopus oryzae Myrmecridium schulzeri Cluster 154 Cystobasidium slooffiae Vishniacozyma victoriae
0.012 0.009 0.009 0.009 0.009 0.009 0.009 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.374 0.000 0.000 0.000 0.000 0.000 0.294 0.287 0.138 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.359 0.318 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 48.841 1.847 1.667 1.623 1.623 1.491 1.169 0.931 0.848 0.443 0.381 0.336 0.329 0.329 0.329 0.291 0.284
Phanerochaete sp. BAB‐4915 Spegazzinia tessarthra Parastagonospora avenae Entoloma terreum Coprinopsis semitalis Cluster 121 Blumeria graminis Gloeocystidiellum kenyense Cluster 70 Burgoa anomala Penicillium citrinum Brassica napus Aspergillus penicillioides Lepista nuda Verrucocladosporium dirinae Vibrissea flavovirens Vaginatispora fuckelii Basidiomycota sp. NFLI 2000‐11 Cluster 78 Mercurialis canariensis Wallemia mellicola Aspergillus versicolor Deconica citrispora Cutaneotrichosporon moniliiform Penicillium pusillum Aleurodiscus thailandicus Trebouxia sp. 20 GS‐2016 Plantago coronopus Fuscoporia ferruginosa Cluster 43 Plantago lagopus Elymus tauri Cluster 48 Prunus kansuensis Cluster 5 Cynodon pascuus Malassezia obtusa Cystofilobasidium macerans Cluster 30 Lecanicillium muscarium Cluster 28 Fraxinus angustifolia Polyporus tuberaster Lecanicillium attenuatum Alternaria infectoria Neottiosporina paspali Kazachstania barnettii Yarrowia galli
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.249 0.239 0.235 0.235 0.235 0.225 0.221 0.218 0.211 0.208 0.204 0.183 0.183 0.176 0.173 0.173 0.170 0.163 0.163 0.159 0.142 0.131 0.128 0.125 0.114 0.114 0.107 0.104 0.104 0.090 0.059 0.055 0.052 0.048 0.038 0.031 0.031 0.028 0.024 0.024 0.024 0.021 0.014 0.010 0.007 0.003 0.003 0.003
Trichoderma lixii Penicillium rubens Sclerotinia sp. 5/97‐18 Cynodon dactylon Cladosporium sp. 2a_co2 Grosmannia olivacea Cystobasidium sp. Cystobasidium minutum Rhizoctonia sp. FA59209 Lactococcus lactis fungal sp. suzu‐11 Alternaria alternata Penicillium aurantiogriseum Lophiostoma macrostomum Metschnikowia aff. pulcherrima Shannon Value CHAO1 Richness Estimation
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.257 82
0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 2.423 178
or ons (<1% of all the species detected) per library. ach library. re shaded in grey.
Balance of the number of proteins and peptides in SWATH proteomic analysis of this study, for wh was obtained and to which were then applied quality filters (described in DIB methodology) to obt
Protein extract type
Myofibrillar Sarcoplasmic
Attempted Quantification (Identified)
Quantified Proteins Peptides
Quantified w Proteins
Proteins
Peptides
190
1359
180
948
178
337
1846
280
1231
277
hich the quantitative analysis was attempted, tain confident quantitative values.
with confidence Peptides 927 1216
,
Table 12 ‐ Worksheet Title Proteins_Itotal Proteins Peptides FDR Area ‐ ions Area ‐ proteins
Worksheet Description List of all the proteins quantified by SWATH with quantification after to normalization (quantitative values multiplied by 10,000) List of all the proteins quantified by SWATH before normalization List of all the peptides used for protein quantification with their respective intensity values FDR analysis of all the transitions used for protein quantification. List of all the transitions monitered with their respective intensity values List of all quantified proteins from SWATH plug‐in in peakview. The sum of the intensity of all proteins per sample is depicted and was used to normalyze the protein values in "Proteins_Itotal"
Table 13 ‐ Worksheet Title Proteins_Itotal Proteins Peptides FDR Area ‐ ions Area ‐ proteins
Worksheet Description List of all the quantified proteins with quantitative values after to normalyzation (quantitative values multiplied by 10.000) List of all the quantified proteins with quantitative values prior to normalyzation List of all the peptides used for protein quantification with their respective intensity values FDR analysis of all the transitions used for protein quantification. List of all the transitions monitered with their respective intensity values List of all quantified proteins from SWATH plug‐in in peakview. The sum of the intensity of all proteins per sample is depicted and was used to normalyze the protein values in "Proteins_Itotal"
Table 14 ‐ Worksheet Title 38 dif Balance of Protein numbers
Worksheet Description List of proteins whose levels changed more than 2‐fold with high‐pressure (HP) and/or storage time in myofibrillar extracts identified by SWATH General balance of the number of proteins in myofibrillar extracts
Table 15 ‐ Worksheet Title 263 dif Balance of Protein numbers
Worksheet Description List of proteins with a more than 2‐fold change in concentration with high‐ pressure (HP) and/or storage time in sarcoplasmic extract and identified by SWATH proteomics General balance of the number of proteins in sarcoplasmic extract
Table 2‐ The main bacterial genera detected by 16s rRNA gene sequencing of control sea bass fille The relative proportion of a given genera relative to the total reads identified in each library is shown as a Only genera detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion).
Genus Shewanella Acinetobacter Flavobacterium Pseudomonas Psychrobacter Chryseobacterium No Hit Aeromonas Rheinheimera Glutamicibacter Pseudoalteromonas Janthinobacterium Carnobacterium Myroides Paeniglutamicibacter Lactococcus Brochothrix Cluster 848
C1 41.3 16.0 13.7 7.7 5.6 2.7 1.3 0.9 0.9 0.4 0.4 0.3 0.1 0.1 0.0 0.0 0.0 0.0
C11 21.6 1.7 2.2 28.7 0.3 0.0 0.2 31.4 0.0 0.0 0.0 2.2 2.2 1.1 0.0 5.4 1.3 0.0
HP1 17.3 27.0 19.1 3.0 5.5 4.4 2.2 0.4 1.5 1.0 1.3 0.1 0.1 0.2 0.1 0.0 0.0 1.2
HP11 3.8 7.1 8.8 57.0 0.9 2.2 1.0 1.0 0.4 0.2 0.5 1.8 0.1 0.0 1.0 0.2 0.1 0.0
HP67 0.2 0.2 0.6 70.3 0.0 0.2 0.2 0.0 0.0 0.0 0.0 26.6 0.0 0.0 0.0 0.0 0.0 0.0
ets or those subject to high‐pressure (HP). percentage.
Table 3‐ List of all the bacterial genera detected by 16S rRNA gene sequencing in sea bass fillets including genera present at a low proportions (<1% of all the genera detected) per library. Values represent the relative proportion of each genus relative to the total reads identified in each libra All detected genera are listed but those at < 1% of the total microbiome in each library are shaded in gr
Genus Shewanella Acinetobacter Flavobacterium Pseudomonas Psychrobacter Chryseobacterium No Hit Aeromonas Rheinheimera Cluster 861 Epilithonimonas Comamonas Pseudoalteromonas Glutamicibacter Nocardia Stenotrophomonas Arthrobacter Pedobacter Janthinobacterium Massilia Corynebacterium Cutibacterium Streptococcus Brevundimonas Staphylococcus Pseudarthrobacter Cluster 22 Ralstonia Acidovorax Carnobacterium Cluster 1508 Sphingobacterium Paracoccus Variovorax Snodgrassella Riemerella Rothia Duganella Rhodoferax Neisseria Micrococcus Paraburkholderia
C1 41.286 16.004 13.741 7.717 5.569 2.747 1.295 0.940 0.930 0.761 0.534 0.501 0.354 0.353 0.330 0.319 0.301 0.280 0.252 0.231 0.200 0.182 0.182 0.172 0.171 0.171 0.167 0.167 0.163 0.148 0.134 0.122 0.112 0.111 0.089 0.085 0.082 0.082 0.078 0.074 0.074 0.074
C11 21.565 1.726 2.190 28.673 0.309 0.044 0.154 31.362 0.004 0.000 0.021 0.018 0.000 0.000 0.014 0.004 0.164 0.046 2.230 0.008 0.000 0.004 0.004 0.000 0.004 0.014 0.000 0.000 0.004 2.249 0.000 0.079 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000
HP1 17.294 27.023 19.082 3.000 5.522 4.429 2.196 0.423 1.503 0.000 0.187 0.611 1.343 1.044 0.379 0.410 0.325 0.338 0.085 0.088 0.560 0.727 0.455 0.213 0.424 0.308 0.000 0.491 0.240 0.107 0.000 0.160 0.673 0.000 0.183 0.330 0.178 0.013 0.076 0.151 0.254 0.325
HP11 3.773 7.113 8.846 57.041 0.923 2.202 0.989 1.025 0.385 0.000 0.231 0.110 0.531 0.165 0.118 0.216 0.361 0.236 1.753 0.008 0.606 0.514 0.522 0.177 0.659 0.981 0.000 0.024 0.028 0.082 0.000 0.051 0.228 0.012 0.122 0.055 0.185 0.004 0.031 0.141 0.436 0.000
HP67 0.185 0.181 0.624 70.338 0.008 0.233 0.181 0.032 0.008 0.000 0.004 0.004 0.004 0.048 0.685 0.000 0.125 0.016 26.586 0.012 0.000 0.000 0.076 0.004 0.004 0.024 0.000 0.000 0.008 0.012 0.000 0.028 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.004 0.000
Alkalibacterium Pseudochrobactrum Cluster 858 Myroides Cluster 44 Leucobacter Rhodanobacter Peptoniphilus Shinella Fluviicola Bacteroides Weissella Paeniglutamicibacter Cluster 990 Cedecea Haemophilus Cluster 734 Gelidibacter Diaphorobacter Cluster 477 Moraxella Devosia Trichococcus Lactobacillus Halomonas [0]Bacteria;[1]Proteoba Leptotrichia Cluster 196 Cluster 726 Lactococcus Polaromonas Cluster 811 Pseudoxanthomonas Bacteriovorax Lysobacter Cluster 891 Actinomyces Chishuiella Cluster 19 Cluster 738 Kocuria Dermacoccus Moheibacter Porphyromonas Xenophilus Roseomonas Pigmentiphaga Bosea
0.071 0.067 0.067 0.060 0.059 0.056 0.056 0.056 0.052 0.052 0.052 0.048 0.048 0.048 0.045 0.045 0.045 0.041 0.041 0.041 0.037 0.037 0.037 0.037 0.034 0.033 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.026 0.026 0.026 0.026 0.026 0.023 0.022 0.019 0.019 0.019 0.019 0.019
0.000 0.004 0.000 1.056 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.025 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.008 0.000 0.000 0.000 0.000 0.000 5.373 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.473 0.009 0.000 0.174 0.000 0.209 0.179 0.004 0.004 0.085 0.182 0.187 0.089 0.000 0.254 0.103 0.000 0.045 0.035 0.000 0.174 0.036 0.085 0.159 0.075 0.000 0.000 0.000 0.000 0.013 0.000 0.000 0.013 0.004 0.040 0.000 0.058 0.049 0.000 0.000 0.088 0.098 0.004 0.035 0.013 0.013 0.000 0.000
0.129 0.059 0.000 0.024 0.000 0.287 0.055 0.028 0.004 0.027 0.032 0.573 1.044 0.000 0.282 0.083 0.000 0.024 0.040 0.000 0.565 0.055 0.047 0.702 0.083 0.043 0.028 0.000 0.000 0.173 0.000 0.000 0.008 0.000 0.004 0.000 0.051 0.016 0.000 0.000 0.275 0.106 0.008 0.102 0.008 0.008 0.000 0.004
0.008 0.000 0.000 0.000 0.000 0.274 0.004 0.000 0.004 0.000 0.008 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.004 0.000 0.004 0.000 0.000 0.000 0.000
Luteimonas Gemella Leclercia Chondromyces Sphingomonas Lysinimonas Alcanivorax Alicycliphilus Rhodovulum Gemmobacter Fusobacterium Vagococcus Methylotenera Clostridium Hyphomicrobium Rhodococcus Prevotella Sanguibacter Pararhizobium Blastococcus Vibrio Facklamia Brucella Brochothrix Achromobacter Amaricoccus Bdellovibrio Alloprevotella Geothrix Bifidobacterium Veillonella Modestobacter Sinorhizobium Methylocystis Methylobacterium Bacillus Undibacterium Simplicispira Enterococcus Pantoea Capnocytophaga Dietzia Ramlibacter Brachybacterium Asaia Albirhodobacter Marinicella Finegoldia
0.018 0.018 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.014 0.014 0.012 0.012 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.007 0.007 0.007 0.007 0.007 0.007
0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.085 0.004 0.040 0.004 0.000 0.000 0.000 0.000 0.000 0.011 0.000 0.000 1.337 0.000 0.000 0.000 0.000 0.011 0.000 0.000 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.008 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.048 0.120 0.000 0.000 0.062 0.000 0.013 0.049 0.000 0.036 0.004 0.000 0.008 0.000 0.000 0.035 0.017 0.018 0.004 0.000 0.085 0.009 0.000 0.004 0.022 0.004 0.009 0.000 0.004 0.013 0.004 0.000 0.004 0.004 0.039 0.000 0.030 0.062 0.027 0.027 0.013 0.026 0.000 0.075 0.085 0.018 0.000 0.000
0.008 0.059 0.000 0.000 0.044 0.000 0.024 0.098 0.012 0.004 0.035 0.004 0.008 0.020 0.012 0.024 0.020 0.012 0.004 0.000 0.040 0.024 0.000 0.114 0.008 0.000 0.008 0.016 0.008 0.146 0.040 0.004 0.000 0.000 0.071 0.051 0.000 0.004 0.008 0.016 0.032 0.020 0.000 0.086 0.043 0.004 0.000 0.078
0.004 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000
Exiguobacterium Peredibacter Rosenbergiella Sulfuricella Erysipelothrix Granulicatella Methylopila Nitrosospira Luteolibacter Pseudorhodoferax Soonwooa Limnohabitans Escherichia Taibaiella Buttiauxella Spirosoma Rhizomicrobium Sphingopyxis Jiangella Thermomonas Pleomorphomonas Marinilactibacillus Nocardioides Paludibacterium Curvibacter Abiotrophia Chiayiivirga Deinococcus Leptonema Pelomonas Anaerococcus Methylophilus Proteiniphilum Streptomyces Hafnia Chitinophaga Myxococcus Planococcus Amphritea Thauera Morganella Colwellia Aliidiomarina Xylella Thermicanus Niastella Paraferrimonas Giesbergeria
0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.004 0.000 0.011 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.004 0.064 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.031 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.022 0.013 0.053 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.000 0.000 0.009 0.004 0.000 0.009 0.009 0.000 0.000 0.018 0.058 0.000 0.000 0.031 0.112 0.000 0.000 0.000 0.000 0.000 0.000 0.022 0.000 0.000 0.000 0.000 0.000 0.000
0.012 0.000 0.000 0.000 0.000 0.024 0.000 0.000 0.000 0.000 0.012 0.000 0.224 0.004 0.000 0.000 0.000 0.012 0.000 0.008 0.000 0.000 0.083 0.000 0.000 0.039 0.000 0.004 0.000 0.008 0.059 0.000 0.000 0.000 0.039 0.000 0.000 0.000 0.000 0.004 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Blastocatella Ahrensia Alcaligenes Phycicoccus Ochrobactrum Leptothrix Methylovirgula Elizabethkingia Sinibacillus Pseudopropionibacteriu Dolosigranulum Actibacter Hymenobacter Thioprofundum Kushneria Arcobacter Oxalicibacterium Rubellimicrobium Photobacterium Romboutsia Lautropia Bradyrhizobium Mesocricetibacter Zymobacter Cellulophaga Tetragenococcus Microbacterium Tahibacter Rubrivivax Rudaea Alkanindiges Leuconostoc Cluster 848 Cluster 25 Cluster 1415 Cluster 844 Cluster 975 Cluster 867 Knoellia Cluster 697 Cluster 650 Agreia Rhizobium Cluster 56 Pseudoclavibacter Cluster 793 Cluster 37 Cluster 27
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.013 0.000 0.004 0.116 0.000 0.018 0.036 0.000 0.000 0.027 0.000 0.009 0.000 0.000 0.022 0.000 0.013 0.018 0.000 0.009 0.004 0.000 0.000 0.000 0.000 0.030 0.000 0.009 0.009 0.017 0.004 1.174 0.362 0.335 0.214 0.094 0.094 0.089 0.085 0.076 0.067 0.058 0.058 0.054 0.054 0.049 0.049
0.000 0.000 0.000 0.035 0.000 0.000 0.000 0.016 0.000 0.000 0.008 0.000 0.110 0.004 0.000 0.004 0.000 0.043 0.008 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.047 0.000 0.000 0.008 0.024 0.016 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.040 0.000 0.012 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000
Cluster 439 Brevibacterium Cluster 785 Cluster 240 Cluster 57 Bavariicoccus Kluyvera Cluster 847 Cluster 611 Cluster 18 Serratia Nesterenkonia Ancylobacter Aquihabitans Peptoanaerobacter Delftia Kroppenstedtia Aeromicrobium Cardiobacterium Dyadobacter Geminicoccus Gemmatimonas Skermanella Marinomonas Negativicoccus Pseudorhodobacter Selenomonas Frondihabitans Mucilaginibacter Peptostreptococcus Salinicoccus Uruburuella Brachymonas Hydrogenophaga Altererythrobacter Actinokineospora Desulfovibrio Dysgonomonas Dermabacter Citrobacter Labilithrix Subsaxibacter Pseudonocardia Caulobacter Dialister Tetrasphaera Varibaculum Kineococcus
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.125 0.000 0.000 0.000 0.379 0.000 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.049 0.044 0.040 0.040 0.036 0.036 0.036 0.036 0.031 0.031 0.031 0.027 0.027 0.022 0.022 0.022 0.022 0.022 0.018 0.018 0.018 0.018 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.008 0.009 0.009 0.009 0.009 0.009 0.008 0.009 0.009 0.009 0.009 0.009 0.008 0.009 0.009
0.000 0.067 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.027 0.000 0.004 0.000 0.012 0.000 0.004 0.000 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.004 0.000 0.000 0.012
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Dokdonella Gordonia Gibbsiella Roseicitreum Bizionia Dongia Zhihengliuella Kurthia Parachlamydia Halodurantibacterium Rouxiella Mycobacterium [0]Bacteria;[1]Firmicute Cylindrospermum Polynucleobacter Hydrobacter Opitutus Cytophaga Cystobacter Hydrogenophilus Salinisphaera Rubritalea Novosphingobium Kingella Aridibacter Cloacibacterium Sediminibacterium Pectobacterium Herpetosiphon Euzebya Paludibaculum Cellulomonas Empedobacter Marmoricola Proteiniclasticum Nostoc Obesumbacterium Ewingella Providencia Melissococcus Salmonella Ichthyenterobacterium Terrimonas Marinobacter Gallionella Niveispirillum Halochromatium Azotobacter
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.082 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.232 0.093 0.018 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.009 0.009 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.008 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.082 0.000 0.000 0.067 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.004
0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.012
Aquisalimonas Enterobacter Balneatrix Erwinia Halothiobacillus Luteibacter Oceanospirillum Azomonas Spirillum Zoogloea Cluster 805 Cluster 633 Siccibacter Rivibacter Agrobacterium Cluster 7 Cluster 375 Cluster 799 Amnibacterium Galbitalea Cluster 1406 Cluster 638 Yersinia Cluster 632 Calothrix Cluster 603 Leucothrix Craurococcus Kytococcus Porphyrobacter Rudaeicoccus Herbaspirillum Adhaeribacter Sphingobium Acaricomes Conchiformibius Bergeyella Blastochloris Sagittula Georgenia Parascardovia Phaselicystis Sandaracinobacter Aerococcus Nitratireductor Geodermatophilus Thermus Rhizobacter
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.228 0.145 0.145 0.110 0.106 0.075 0.071 0.055 0.055 0.051 0.051 0.035 0.035 0.035 0.036 0.031 0.020 0.020 0.020 0.020 0.016 0.016 0.012 0.012 0.012 0.012 0.012 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008
0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Labrys Alkalibacter Anaerobacillus Coprococcus Acidibacter Nitrospira Celeribacter Mariniflexile Desemzia Geobacter Pontibacter Weeksella Xanthomonas Domibacillus Nitrosococcus Kastovskya Jannaschia Kineosporia Rhodobacter Lewinella Filifactor Halospirulina Eubacterium Mycetocola Janibacter Pasteurella Acidipropionibacterium Aliiroseovarius Lachnoclostridium [0]Bacteria;[1]Firmicute Balneola Tomitella Hoeflea Flavisolibacter Methylobacillus Methyloversatilis Azovibrio Intestinimonas Ohtaekwangia Silanimonas Lysinibacter Tolumonas Modicisalibacter Thalassomonas Pandoraea Hydrocarboniphaga Ferruginibacter Caloramator
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
s, ary. rey.
Table 4‐ The main bacterial species detected (>1% of all the species detected) by 16S rRNA ge The relative proportion of a given species relative to the total reads identified in each library is shown Only species detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from g Note that the HP treatment caused an approximately 3‐fold reduction in Shewanella baltica . In contrast, A. Johnsonii proportions were duplicated with HP treatments.
Main species Shewanella baltica Acinetobacter johnsonii Shewanella xiamenensis Shewanella putrefaciens Acinetobacter radioresistens Flavobacterium antarcticum Flavobacterium ummariense Flavobacterium qiangtangense Pseudomonas gessardii Flavobacterium dongtanense Pseudomonas asturiensis Psychrobacter pulmonis No Hit Shewanella denitrificans Chryseobacterium antarcticum Pseudomonas tolaasii Pseudomonas japonica Flavobacterium granuli Carnobacterium jeotgali Pseudomonas deceptionensis Shewanella profunda Acinetobacter junii Aeromonas salmonicida Lactococcus piscium Brochothrix thermosphacta Pseudomonas migulae Pseudomonas azotoformans Pseudomonas marginalis Aeromonas hydrophila Cluster 848 Janthinobacterium lividum Paeniglutamicibacter antarcticu Pseudomonas vranovensis Aeromonas rivuli Pseudomonas psychrophila Janthinobacterium agaricidamn Myroides profundi
C1 25.498 10.989 6.787 6.505 3.433 3.225 3.065 2.991 2.772 2.709 2.583 2.03 1.295 0.957 0.609 0.434 0.267 0.245 0.148 0.108 0.078 0.03 0.026 0.022 0.011 0.011 0.007 0.004 0.004 0 0 0 0 0 0 0 0
C11 15.26 0.143 2.652 1.698 1.122 0.011 0.014 0.032 8.693 0.004 0.018 0 0.154 0.011 0.007 0.1 2.324 2.059 2.098 0.064 1.255 0 1.101 5.298 1.33 1.223 0.021 1.437 15.31 0 0.05 0.025 0.004 13.23 12.611 2.13 1.004
HP1 9.709 22.05 2.089 2.232 1.848 2.29 5.236 2.116 0.004 6.539 0.004 2.468 2.196 2.504 1.299 0.018 0.241 0.192 0.094 0 0.004 1.214 0.138 0.009 0.004 0.004 1.004 0 0 1.174 0.085 0.085 0.004 0 0 0 0
HP11 1.989 5.39 0.635 0.431 0.608 1.585 2.224 1.236 35.156 2.052 0 0.016 0.989 0.6 0.647 0.51 0 0.18 0 1.483 0.012 0.008 0.934 0.173 0.114 0.012 16.754 0.024 0 0 1.71 1.04 1.043 0 0 0.004 0.012
HP67 0.101 0.141 0.004 0 0.024 0.06 0.214 0.085 0.028 0.137 0.004 0 0.181 0.032 0.097 70.021 0 0 0.012 0 0.04 0 0 0 0 0 0.012 0.032 0 0 0.895 0 0 0 0.008 25.215 0
ene sequencing. as a percentage. grey (low proportion) to red (high proportion).
Table 5‐ All bacterial species detected by 16S rRNA gene sequencing in control or HPP sea bass fill Values represent the relative proportion of each species relative to the total reads identified in each library All detected species are listed but those at < 1% of the total microbiome in each library are shaded in grey.
Main species Shewanella baltica Acinetobacter johnsonii Shewanella xiamenensis Shewanella putrefaciens Acinetobacter radioresistens Flavobacterium antarcticum Flavobacterium ummariense Flavobacterium qiangtangense Pseudomonas gessardii Flavobacterium dongtanense Pseudomonas asturiensis Psychrobacter pulmonis No Hit Shewanella denitrificans Aeromonas veronii Cluster 861 Psychrobacter arenosus Psychrobacter arcticus Shewanella hafniensis Psychrobacter piscatorii Flavobacterium frigidimaris Psychrobacter cryohalolentis Chryseobacterium antarcticum Chryseobacterium jeonii Epilithonimonas lactis Chryseobacterium balustinum Acinetobacter guillouiae Pseudomonas tolaasii Pseudomonas peli Acinetobacter kyonggiensis Chryseobacterium carnis Rheinheimera baltica Nocardia coeliaca Glutamicibacter arilaitensis Comamonas kerstersii Pseudomonas caeni Psychrobacter cibarius Flavobacterium gelidilacus Pseudoalteromonas haloplanktis Pseudomonas japonica Arthrobacter psychrochitiniphilus Rheinheimera aestuari Janthinobacterium svalbardensis
C1 25.498 10.989 6.787 6.505 3.433 3.225 3.065 2.991 2.772 2.709 2.583 2.030 1.295 0.957 0.798 0.761 0.742 0.716 0.693 0.672 0.635 0.616 0.609 0.568 0.527 0.512 0.482 0.434 0.430 0.427 0.412 0.371 0.330 0.327 0.308 0.286 0.278 0.275 0.275 0.267 0.260 0.252 0.252
C11 15.260 0.143 2.652 1.698 1.122 0.011 0.014 0.032 8.693 0.004 0.018 0.000 0.154 0.011 0.000 0.000 0.000 0.000 0.436 0.004 0.000 0.004 0.007 0.004 0.021 0.011 0.100 0.100 0.000 0.193 0.004 0.000 0.014 0.000 0.011 0.004 0.214 0.007 0.000 2.324 0.157 0.000 0.050
HP1 9.709 22.050 2.089 2.232 1.848 2.290 5.236 2.116 0.004 6.539 0.004 2.468 2.196 2.504 0.000 0.000 0.750 0.790 0.245 0.549 0.567 0.527 1.299 0.759 0.187 0.719 0.143 0.018 0.375 0.246 0.741 0.812 0.379 0.853 0.326 0.103 0.000 0.420 0.121 0.241 0.308 0.022 0.000
HP11 1.989 5.390 0.635 0.431 0.608 1.585 2.224 1.236 35.156 2.052 0.000 0.016 0.989 0.600 0.008 0.000 0.004 0.063 0.047 0.122 0.522 0.133 0.647 0.349 0.231 0.173 0.216 0.510 0.000 0.067 0.333 0.126 0.118 0.059 0.078 0.016 0.102 0.302 0.012 0.000 0.333 0.000 0.039
HP67 0.101 0.141 0.004 0.000 0.024 0.060 0.214 0.085 0.028 0.137 0.004 0.000 0.181 0.032 0.024 0.000 0.000 0.000 0.004 0.004 0.044 0.000 0.097 0.032 0.004 0.024 0.004 70.021 0.000 0.000 0.064 0.004 0.685 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.117 0.000 0.476
Flavobacterium granuli Shewanella glacialipiscicola Psychrobacter maritimus Pseudomonas flavescens Chryseobacterium yonginense Massilia aurea Pseudomonas helmanticensis Shewanella arctica Kim et al. 201 Cutibacteirum acnes Chryseobacterium lactis Stenotrophomonas terrae Pseudarthrobacter phenanthreni Cluster 22 Staphylococcus epidermidis Comamonas koreensis Acinetobacter lwoffii Carnobacterium jeotgali Rheinheimera soli Stenotrophomonas rhizophila Cluster 1508 Psychrobacter lutiphocae Acidovorax radicis Flavobacterium tegetincola Flavobacterium caeni Ralstonia pickettii Shewanella seohaensis Pseudomonas deceptionensis Corynebacterium kroppenstedtii Shewanella morhuae Acinetobacter baumannii Pseudomonas syringae Chryseobacterium polytrichastri Aeromonas aquatica Acinetobacter qingfengensis Streptococcus oralis Snodgrassella alvi Sphingobacterium anhuiense Riemerella columbipharyngis Rhodoferax ferrireducens Shewanella profunda Variovorax ginsengisoli Micrococcus aloeverae Rheinheimera aquimaris Flavobacterium oryzae Pseudoalteromonas prydzensis Rothia endophytica Pseudomonas fluorescens Pedobacter namyangjuensis
0.245 0.241 0.226 0.226 0.200 0.193 0.186 0.182 0.178 0.174 0.174 0.171 0.167 0.167 0.163 0.160 0.148 0.148 0.134 0.134 0.134 0.126 0.126 0.122 0.111 0.111 0.108 0.100 0.097 0.096 0.093 0.093 0.093 0.093 0.093 0.089 0.089 0.085 0.078 0.078 0.078 0.074 0.074 0.074 0.071 0.071 0.071 0.071
2.059 0.025 0.000 0.093 0.000 0.000 0.021 0.007 0.004 0.000 0.000 0.014 0.000 0.004 0.007 0.050 2.098 0.000 0.004 0.000 0.004 0.004 0.004 0.000 0.000 0.046 0.064 0.000 0.064 0.089 0.318 0.004 0.483 0.000 0.004 0.004 0.000 0.000 0.000 1.255 0.000 0.000 0.000 0.000 0.000 0.000 0.222 0.000
0.192 0.049 0.129 0.036 0.295 0.000 0.103 0.379 0.723 0.004 0.000 0.308 0.000 0.058 0.254 0.299 0.094 0.357 0.165 0.000 0.116 0.129 0.210 0.018 0.362 0.004 0.000 0.272 0.018 0.121 0.201 0.058 0.107 0.004 0.219 0.183 0.000 0.330 0.076 0.004 0.000 0.000 0.112 0.362 0.004 0.089 0.000 0.000
0.180 0.004 0.341 0.000 0.094 0.000 0.016 0.035 0.510 0.173 0.035 0.981 0.000 0.439 0.000 0.314 0.000 0.090 0.177 0.000 0.008 0.020 0.196 0.020 0.024 0.000 1.483 0.212 0.008 0.004 0.000 0.012 0.024 0.000 0.396 0.122 0.000 0.055 0.031 0.012 0.012 0.318 0.000 0.279 0.000 0.039 0.000 0.000
0.000 0.004 0.004 0.000 0.000 0.004 0.012 0.000 0.000 0.008 0.000 0.024 0.000 0.000 0.000 0.008 0.012 0.000 0.000 0.000 0.000 0.004 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.000 0.000 0.004 0.000 0.040 0.000 0.000 0.000 0.024 0.000 0.000 0.028 0.004
Brevundimonas bullata Pseudochrobactrum lubricantis Cluster 858 Alkalibacterium olivapovliticus Duganella phyllosphaerae Paraburkholderia caledonica Cluster 44 Paracoccus aminophilus Brevundimonas vesicularis Rhodanobacter glycinis Myroides phaeus Streptococcus parauberis Ralstonia syzygii Acinetobacter gerneri Bacteroides graminisolvens Shinella zoogloeoides Acinetobacter haemolyticus Fluviicola taffensis Shewanella basaltis Leucobacter salsicius Pedobacter ginsengiterrae Peptoniphilus gorbachii Chryseobacterium chaponense Flavobacterium akiainvivens Paeniglutamicibacter kerguelensi Cluster 990 Pedobacter nyackensis Flavobacterium anatoliense Neisseria mucosa Cluster 734 Paracoccus aminovorans Cedecea lapagei Cluster 477 Pseudomonas brenneri Weissella viridescens Rheinheimera chironomi Diaphorobacter aerolatus Acinetobacter beijerinckii Gelidibacter mesophilus Moraxella osloensis Corynebacterium accolens Trichococcus pasteurii Acidovorax soli Chryseobacterium indoltheticum Psychrobacter adeliensis Pseudomonas endophytica Variovorax soli Flavobacterium ponti
0.071 0.067 0.067 0.067 0.063 0.059 0.059 0.059 0.056 0.056 0.056 0.056 0.056 0.056 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.048 0.048 0.048 0.045 0.045 0.045 0.045 0.045 0.041 0.041 0.041 0.041 0.041 0.041 0.041 0.037 0.037 0.037 0.037 0.033 0.033 0.033 0.033 0.033
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.018 0.000 0.000 0.014 0.000 0.000 0.000 0.000 0.043 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.311 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.004 0.322 0.000 0.000
0.107 0.009 0.000 0.442 0.013 0.254 0.000 0.018 0.062 0.179 0.161 0.134 0.129 0.370 0.165 0.000 0.022 0.076 0.018 0.004 0.000 0.000 0.067 0.062 0.004 0.000 0.004 0.089 0.107 0.000 0.004 0.254 0.000 0.027 0.187 0.004 0.022 0.210 0.045 0.170 0.170 0.085 0.098 0.004 0.067 0.027 0.000 0.553
0.004 0.059 0.000 0.129 0.000 0.000 0.000 0.008 0.000 0.055 0.000 0.039 0.000 0.024 0.024 0.000 0.004 0.027 0.000 0.275 0.012 0.000 0.004 0.000 0.004 0.000 0.000 0.043 0.090 0.000 0.000 0.282 0.000 0.667 0.561 0.067 0.020 0.000 0.024 0.565 0.161 0.047 0.008 0.145 0.004 0.027 0.000 0.000
0.004 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.056 0.000 0.000 0.008 0.004 0.000 0.000 0.000 0.270 0.000 0.000 0.004 0.004 0.000 0.000 0.000 0.004 0.004 0.000 0.004 0.000 0.000 0.020 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.008 0.000 0.008
Shewanella amazonensis Devosia submarina Shewanella algae Acinetobacter schindleri Pseudomonas geniculata Cluster 726 Polaromonas naphthalenivorans Bacteriovorax stolpii Acinetobacter gandensis Psychrobacter aquaticus Acinetobacter junii Haemophilus parainfluenzae Pseudoxanthomonas yeongjuens Flavobacterium succinicans Acinetobacter bouvetii Cluster 891 Cluster 811 Lysobacter gummosus Leptotrichia wadei Cluster 196 Arthrobacter stackebrandtii Cluster 738 Glutamicibacter bergerei Chishuiella changwenlii Cluster 19 Pedobacter hartonius Pseudomonas guineae Aeromonas salmonicida Sphingobacterium nematocida Kocuria rhizophila Neisseria cinerea Lactococcus piscium Pseudomonas prosekii Streptococcus sanguinis Moheibacter sediminis Lactobacillus iners Chryseobacterium aahli Pedobacter steynii Actinomyces odontolyticus Psychrobacter alimentarius Xenophilus arseniciresistens Pigmentiphaga soli Dermacoccus abyssi Brevundimonas lenta Bosea robiniae Pedobacter koreensis Flavobacterium terrigena Halomonas lutea
0.033 0.033 0.033 0.033 0.033 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.026 0.026 0.026 0.026 0.026 0.026 0.026 0.026 0.026 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.019 0.019 0.019 0.019 0.019 0.019 0.019 0.019
0.004 0.000 0.014 0.000 0.000 0.000 0.000 0.000 0.000 0.036 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.000 0.000 0.000 0.000 0.000 0.004 1.101 0.000 0.000 0.000 5.298 0.097 0.000 0.000 0.000 0.000 0.046 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.036 0.004 0.040 0.000 0.000 0.000 0.004 0.080 0.009 1.214 0.085 0.000 0.031 0.027 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.183 0.049 0.000 0.000 0.009 0.138 0.058 0.000 0.000 0.009 0.009 0.049 0.004 0.134 0.031 0.089 0.009 0.004 0.013 0.000 0.000 0.022 0.000 0.112 0.013 0.027
0.000 0.000 0.000 0.012 0.043 0.000 0.000 0.000 0.192 0.000 0.008 0.051 0.008 0.027 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.008 0.016 0.000 0.000 0.078 0.934 0.012 0.012 0.000 0.173 0.008 0.008 0.008 0.698 0.008 0.118 0.008 0.008 0.008 0.000 0.000 0.075 0.004 0.090 0.012 0.055
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.048 0.000 0.000 0.000 0.004 0.000 0.012 0.000 0.000 0.000 0.020 0.000 0.000 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000
Massilia niastensis Pedobacter panaciterrae Porphyromonas pasteri Flavobacterium haoranii Leclercia adecarboxylata Acinetobacter puyangensis Massilia eurypsychrophila Gemmobacter intermedius Pseudomonas lini Duganella zoogloeoides Arthrobacter halodurans Pseudomonas chengduensis Chondromyces lanuginosus Rheinheimera hassiensis Alicycliphilus denitrificans Flavobacterium lacus Corynebacterium testudinoris Chryseobacterium anthropi Chryseobacterium soldanellicola Aeromonas lacus Alcanivorax dieselolei Lysinimonas soli Rhodovulum kholense Brevundimonas naejangsanensis Corynebacterium pseudodiphther Roseomonas oryzae Acinetobacter septicus Fusobacterium nucleatum Amaricoccus tamworthensis Veillonella parvula Prevotella jejuni Chryseobacterium piscicola Gemella taiwanensis Flavobacterium hibernum Brochothrix thermosphacta Brevundimonas poindexterae Pseudomonas koreensis Facklamia languida Comamonas jiangduensis Stenotrophomonas chelatiphaga Sanguibacter keddieii Bifidobacterium longum Pseudomonas migulae Psychrobacter aquimaris Blastococcus endophyticus Pararhizobium polonicum Geothrix fermentans Luteimonas huabeiensis
0.019 0.019 0.019 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011
0.000 0.000 0.000 0.000 0.000 0.011 0.000 0.000 0.014 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.390 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.025 1.330 0.000 0.118 0.000 0.000 0.000 0.000 0.000 1.223 0.000 0.000 0.000 0.011 0.000
0.062 0.125 0.031 0.040 0.000 0.009 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.009 0.049 0.036 0.062 0.062 0.000 0.013 0.013 0.000 0.000 0.004 0.000 0.000 0.027 0.004 0.000 0.000 0.000 0.000 0.116 0.000 0.004 0.000 0.000 0.009 0.009 0.009 0.000 0.000 0.004 0.004 0.000 0.004 0.004 0.000
0.004 0.000 0.090 0.004 0.000 0.027 0.000 0.000 0.024 0.004 0.004 0.000 0.000 0.055 0.098 0.031 0.000 0.000 0.008 0.016 0.020 0.000 0.012 0.000 0.012 0.000 0.027 0.035 0.000 0.000 0.004 0.020 0.008 0.039 0.114 0.098 0.008 0.016 0.008 0.000 0.012 0.130 0.012 0.000 0.000 0.004 0.008 0.000
0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.004
Modestobacter caceresii Achromobacter aegrifaciens Rhodococcus cerastii Alloprevotella rava Corynebacterium xerosis Psychrobacter sanguinis Comamonas testosteroni Vibrio anguillarum Halomonas boliviensis Brucella papionis Comamonas terrigena Shewanella chilikensis Luteolibacter pohnpeiensis Sphingopyxis fribergensis Rheinheimera pacifica Buttiauxella warmboldiae Erysipelothrix inopinata Pseudomonas anguilliseptica Spirosoma fluviale Sphingomonas ginsenosidivorax Finegoldia magna Ramlibacter solisilvae Vagococcus fessus Pseudorhodoferax aquiterrae Marinicella litoralis Escherichia fergusonii Epilithonimonas tenax Pseudomonas kuykendallii Pseudomonas mucidolens Rheinheimera arenilitoris Psychrobacter immobilis Corynebacterium minutissimum Methylotenera versatilis Taibaiella chishuiensis Asaia siamensis Pseudomonas fragi Flavobacterium urumqiense Paraburkholderia sabiae Limnohabitans planktonicus Psychrobacter faecalis Vagococcus carniphilus Neisseria oralis Pedobacter lotistagni Rothia dentocariosa Lactobacillus rhamnosus Pseudomonas putida Sphingobacterium faecium Chryseobacterium hispanicum
0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.008 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007
0.000 0.000 0.000 0.000 0.000 0.039 0.000 0.007 0.000 0.000 0.000 0.046 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.036 0.082 0.000 0.004 0.000 0.004 0.000 0.000 0.025 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.079 0.000
0.000 0.022 0.031 0.000 0.000 0.009 0.013 0.045 0.031 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.000 0.009 0.000 0.009 0.000 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.000 0.125 0.018 0.000 0.004 0.000 0.085 0.000 0.000 0.045 0.013 0.040 0.000 0.000 0.000 0.000 0.009 0.000 0.098 0.000
0.000 0.008 0.020 0.016 0.000 0.090 0.000 0.020 0.012 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.078 0.000 0.000 0.000 0.000 0.212 0.000 0.000 0.200 0.004 0.004 0.020 0.008 0.000 0.043 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.106 0.004 0.004 0.039 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.069 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.012 0.000
Rosenbergiella australoborealis Gemella morbillorum Psychrobacter fozii Nitrosospira multiformis Luteimonas abyssi Pseudomonas punonensis Peredibacter starrii Pseudomonas savastanoi Psychrobacter okhotskensis Methylotenera mobilis Bdellovibrio bacteriovorus Pseudomonas composti Haemophilus influenzae Weissella ceti Albirhodobacter marinus Brachybacterium faecium Exiguobacterium sibiricum Soonwooa buanensis Sulfuricella denitrificans Acinetobacter indicus Corynebacterium pyruviciproduce Streptococcus thermophilus Rhizomicrobium electricum Granulicatella elegans Pseudomonas azotoformans Acinetobacter tandoii Methylopila musalis Flavobacterium tructae Rheinheimera aquatica Deinococcus indicus Roseomonas gilardii Alkalibacterium psychrotolerans Dermacoccus profundi Myxococcus macrosporus Rubellimicrobium roseum Methylobacterium populi Niastella yeongjuensis Mesocricetibacter intestinalis Chryseobacterium solani Jiangella mangrovi Hymenobacter psychrophilus Sinorhizobium medicae Thermomonas carbonis Chryseobacterium vietnamense Shewanella dokdonensis Rubrivivax gelatinosus Chryseobacterium humi Flavobacterium noncentrifugens
0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.011 0.000 0.000 0.000 0.000 0.014 0.000 0.018 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.000 0.021 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.004 0.000 0.000 0.040 0.000 0.004 0.000 0.004 0.004 0.009 0.018 0.000 0.000 0.018 0.013 0.022 0.022 0.000 0.000 0.000 0.000 0.000 0.000 1.004 0.022 0.000 0.183 0.000 0.000 0.013 0.000 0.000 0.000 0.013 0.000 0.000 0.000 0.009 0.000 0.000 0.004 0.000 0.000 0.000 0.009 0.000 0.004
0.000 0.000 0.000 0.000 0.008 0.027 0.000 0.000 0.000 0.000 0.000 0.016 0.024 0.012 0.004 0.086 0.008 0.012 0.000 0.000 0.000 0.027 0.000 0.008 16.754 0.016 0.000 0.031 0.000 0.000 0.000 0.000 0.102 0.000 0.039 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Microbacterium pygmaeum Abiotrophia defectiva Cutibacterium granulosum Dolosigranulum pigrum Leptonema illini Thauera aminoaromatica Roseomonas cervicalis Lactococcus plantarum Sinibacillus soli Pseudopropionibacterium propio Flavobacterium columnare Methylocystis echinoides Leuconostoc carnosum Pedobacter jejuensis Devosia psychrophila Enterococcus columbae Simplicispira metamorpha Kocuria kristinae Rothia mucilaginosa Rheinheimera japonica Duganella sacchari Zymobacter palmae Nocardioides daedukensis Lactobacillus songhuajiangensis Sphingomonas panni Pantoea gaviniae Rudaea cellulosilytica Haemophilus paraphrohaemolyti Pseudomonas veronii Rheinheimera mesophila Paracoccus yeei Tahibacter aquaticus Pseudomonas marginalis Corynebacterium tuberculosteari Photobacterium phosphoreum Dietzia alimentaria Haemophilus pittmaniae Hafnia psychrotolerans Flavobacterium micromati Aliidiomarina maris Streptococcus gordonii Chitinophaga costaii Ochrobactrum rhizosphaerae Lactococcus formosensis Romboutsia sedimentorum Flavobacterium urocaniciphilum Pedobacter terrae Halomonas stenophila
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.075 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 1.437 0.000 0.000 0.000 0.000 0.000 0.018 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.009 0.004 0.027 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.049 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.009 0.000 0.013 0.004 0.580 0.000 0.000 0.000 0.000 0.000 0.000 0.112 0.000 0.000 0.000 0.000 0.116 0.000 0.000 0.004 0.004 0.004
0.000 0.039 0.004 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.004 0.000 0.000 0.000 0.024 0.043 0.000 0.000 0.012 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.012 0.000 0.024 0.008 0.000 0.008 0.000 0.039 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.032 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Bacillus alveayuensis Bdellovibrio exovorus Pedobacter arcticus Shewanella decolorationis Enterococcus gallinarum Alkanindiges illinoisensis Clostridium perfringens Corynebacterium suicordis Giesbergeria anulus Hyphomicrobium aestuarii Psychrobacter fulvigenes Phycicoccus badiiscoriae Colwellia aestuarii Acinetobacter seohaensis Simplicispira psychrophila Leucobacter chromiireducens Capnocytophaga leadbetteri Blastocatella fastidiosa Pantoea septica Massilia aerilata Clostridium baratii Proteiniphilum acetatigenes Actinomyces bowdenii Pseudomonas jessenii Planococcus halocryophilus Acinetobacter marinus Sphingomonas starnbergensis Cellulophaga tyrosinoxydans Chryseobacterium treverense Paraburkholderia oxyphila Kushneria pakistanensis Undibacterium squillarum Dietzia aurantiaca Aeromonas hydrophila Arcobacter butzleri Psychrobacter ciconiae Streptomyces klenkii Aeromonas eucrenophila Clostridium subterminale Peptoniphilus coxii Pedobacter himalayensis Chryseobacterium soli Thermicanus aegyptius Paracoccus rhizosphaerae Xylella fastidiosa Chryseobacterium marinum Methylophilus methylotrophus Alcaligenes aquatilis
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 15.310 0.000 0.000 0.000 0.039 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.004 0.018 0.009 0.000 0.000 0.000 0.000 0.013 0.004 0.022 0.004 0.062 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.022 0.000 0.000 0.004 0.018 0.000 0.000 0.000 0.004 0.013 0.000 0.000 0.000 0.004 0.000 0.000
0.000 0.008 0.000 0.000 0.000 0.024 0.004 0.012 0.000 0.000 0.000 0.035 0.008 0.004 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.016 0.020 0.000 0.055 0.000 0.000 0.000 0.008 0.000 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Myroides injenensis Marinilactibacillus psychrotoleran Actibacter sediminis Amphritea atlantica Paludibacterium yongneupense Methylocystis hirsuta Capnocytophaga sputigena Fusobacterium simiae Pseudomonas donghuensis Flavobacterium suncheonense Pseudoalteromonas shioyasakien Flavobacterium ceti Paraferrimonas sedimenticola Leptothrix mobilis Staphylococcus sciuri Flavobacterium cutihirudinis Methylobacterium phyllostachyo Tetragenococcus muriaticus Psychrobacter submarinus Lactobacillus shenzhenensis Pseudoalteromonas arabiensis Elizabethkingia endophytica Corynebacterium durum Flavobacterium lindanitolerans Psychrobacter urativorans Bradyrhizobium japonicum Acinetobacter venetianus Pelomonas saccharophila Sinorhizobium fredii Hyphomicrobium denitrificans Oxalicibacterium solurbis Undibacterium oligocarboniphilu Bacillus horikoshii Methylovirgula ligni Ahrensia kielensis Flavobacterium algicola Chiayiivirga flava Thioprofundum hispidum Lautropia mirabilis Curvibacter delicatus Paraburkholderia phytofirmans Anaerococcus prevotii Morganella morganii Flavobacterium jumunjinense Hyphomicrobium methylovorum Psychrobacter marincola Shewanella mangrovi Pseudomonas oryzihabitans
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.018 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.182 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.013 0.000 0.013 0.018 0.013 0.004 0.000 0.000 0.000 0.000 0.022 0.000 0.000 0.000 0.000 0.000 0.013 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.018 0.013 0.000 0.009 0.000 0.009 0.000 0.004 0.000 0.000 0.031 0.000 0.013 0.000 0.013
0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.012 0.039 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.157 0.000 0.035 0.000 0.000 0.004 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.004 0.000 0.016 0.000 0.012
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Pleomorphomonas diazotrophica Pseudomonas corrugata Cluster 848 Pseudoalteromonas undina Cluster 25 Staphylococcus saprophyticus Chryseobacterium oncorhynchi Cluster 1415 Micrococcus luteus Cluster 844 Leucobacter exalbidus Pseudoalteromonas luteoviolacea Stenotrophomonas humi Acinetobacter tjernbergiae Acinetobacter kookii Dermacoccus nishinomiyaensis Cluster 867 Cluster 975 Knoellia aerolata Aeromonas australiensis Janthinobacterium lividum Cluster 697 Paeniglutamicibacter antarcticus Kocuria palustris Cluster 650 Stenotrophomonas pavanii Paracoccus sanguinis Agreia pratensis Brachybacterium rhamnosum Aeromonas bivalvium Cluster 56 Pseudoclavibacter helvolus Rhizobium skierniewicense Cluster 793 Cluster 439 Cluster 37 Actinomyces naeslundii Cluster 27 Streptococcus salivarius Cluster 240 Cluster 785 Rothia aeria Gemmobacter nectariphilus Cluster 847 Bavariicoccus seileri Brevibacterium ammoniilyticum Cluster 57 Elizabethkingia miricola
0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.157 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.025 0.050 0.000 0.025 0.000 0.000 0.000 0.000 0.000 0.000 0.021 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.732 1.174 0.978 0.362 0.344 0.339 0.335 0.254 0.214 0.205 0.201 0.165 0.138 0.116 0.098 0.094 0.094 0.089 0.085 0.085 0.085 0.085 0.080 0.076 0.071 0.071 0.067 0.062 0.058 0.058 0.054 0.054 0.054 0.049 0.049 0.049 0.049 0.045 0.040 0.040 0.040 0.036 0.036 0.036 0.036 0.036 0.036
0.000 0.027 0.000 0.271 0.000 0.192 0.000 0.000 0.110 0.000 0.012 0.055 0.000 0.039 0.000 0.004 0.000 0.000 0.000 0.012 1.710 0.000 1.040 0.263 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.035 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.016
0.004 0.012 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.895 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Kluyvera intermedia Cluster 18 Alkalibacterium iburiense Cluster 611 Neisseria perflava Serratia quinivorans Anaerococcus octavius Curtobacterium plantarum Anaerococcus hydrogenalis Shewanella gaetbuli Nesterenkonia xinjiangensis Rheinheimera nanhaiensis Ancylobacter polymorphus Sphingomonas psychrolutea Pseudoalteromonas tunicata Aquihabitans daechungensis Microbacterium luteolum Delftia tsuruhatensis Vibrio toranzoniae Eubacterium yurii Paraburkholderia heleia Kroppenstedtia guangzhouensis Aeromicrobium tamlense Photobacterium piscicola Pelomonas puraquae Geminicoccus roseus Vibrio lentus Aeromonas finlandiensis Gemmatimonas aurantiaca Sanguibacter antarcticus Haemophilus parahaemolyticus Dyadobacter beijingensis Lysobacter capsici Rheinheimera muenzenbergensis Cardiobacterium hominis Brevundimonas mediterranea Rheinheimera tilapiae Methylobacterium indicum Salinicoccus qingdaonensis Neisseria elongata Mucilaginibacter sabulilitoris Skermanella stibiiresistens Pseudoxanthomonas wuyuanens Uruburuella suis Marinomonas foliarum Brachymonas denitrificans Corynebacterium amycolatum Acidovorax defluvii
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.125 0.000 0.000 0.000 0.000 0.011 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.036 0.031 0.031 0.031 0.031 0.031 0.027 0.027 0.027 0.027 0.027 0.027 0.027 0.027 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013
0.000 0.000 0.000 0.000 0.039 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.000 0.039 0.027 0.000 0.000 0.000 0.000 0.004 0.008 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.008 0.000 0.000 0.035 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Flavobacterium chungangense Bifidobacterium animalis Staphylococcus warneri Frondihabitans peucedani Acinetobacter calcoaceticus Arcobacter cryaerophilus Selenomonas noxia Diaphorobacter polyhydroxybuty Flavobacterium gyeonganense Sphingomonas pituitosa Arthrobacter agilis Myroides odoratimimus Carnobacterium funditum Undibacterium jejuense Sphingomonas canadensis Peptostreptococcus stomatis Massilia umbonata Negativicoccus succinicivorans Arcobacter venerupis Chryseobacterium contaminans Halomonas kenyensis Caulobacter segnis Subsaxibacter broadyi Prevotella oris Corynebacterium imitans Corynebacterium casei Varibaculum cambriense Lysobacter panaciterrae Pseudorhodobacter aquimaris Alkanindiges hongkongensis Gordonia terrae Labilithrix luteola Dokdonella immobilis Dermabacter hominis Nocardioides kongjuensis Pseudomonas guguanensis Desulfovibrio simplex Fluviicola hefeinensis Flavobacterium seoulense Bacteroides propionicifaciens Pseudonocardia alaniniphila Streptomyces olivoviridis Kineococcus radiotolerans Undibacterium pigrum Staphylococcus devriesei Flavobacterium fluvii Dysgonomonas gadei Enterococcus cecorum
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.008 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009
0.000 0.008 0.004 0.000 0.004 0.000 0.000 0.020 0.000 0.000 0.000 0.012 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.012 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.039 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Massilia namucuonensis Actinokineospora fastidiosa Dialister pneumosintes Altererythrobacter troitsensis Hymenobacter metalli Exiguobacterium aestuarii Comamonas composti Lysobacter brunescens Prevotella intermedia Hydrobacter penzbergensis Hydrogenophaga caeni Dongia mobilis Halodurantibacterium flavum Pseudomonas viridiflava Escherichia marmotae Prevotella loescheii Pseudomonas flexibilis Streptomyces alkalithermotolera Rubritalea spongiae Acinetobacter guangdongensis Cellulomonas massiliensis Flavobacterium frigidarium Chryseobacterium formosense Kingella oralis Chryseobacterium bovis Porphyromonas catoniae Brevibacterium aurantiacum Flavobacterium nitrogenifigens Opitutus terrae Aridibacter famidurans Corynebacterium variabile Cytophaga xylanolytica Acinetobacter gyllenbergii Roseicitreum antarcticum Pseudomonas vranovensis Cloacibacterium haliotis Mycobacterium szulgai Tetrasphaera australiensis Acinetobacter soli Hydrogenophaga taeniospiralis Lactobacillus faecis Sediminibacterium salmoneum Novosphingobium lindaniclasticu Glutamicibacter uratoxydans Citrobacter freundii Marmoricola scoriae Brevibacterium ravenspurgense Amaricoccus kaplicensis
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000
0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.016 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.012 0.008 0.000 0.000 0.000 0.020 0.000 0.137 0.000 1.043 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Lactobacillus delbrueckii Polynucleobacter asymbioticus Bacteroides acidifaciens Lactobacillus suebicus Rhizobium subbaraonis Empedobacter brevis Bizionia paragorgiae Dietzia lutea Glutamicibacter nicotianae Pseudomonas vancouverensis Paludibaculum fermentans Microbacterium ginsengiterrae Bacteroides coagulans Bacteroides cellulosilyticus Undibacterium seohonense Euzebya tangerina Rhodococcus globerulus Salinisphaera shabanensis Psychrobacter namhaensis Corynebacterium falsenii Flavobacterium hauense Citrobacter koseri Tetrasphaera duodecadis Acinetobacter brisouii Kocuria marina Lysobacter concretionis Flavobacterium aquidurense Methylocystis rosea Pseudomonas reinekei Sphingobacterium yanglingense Moraxella nonliquefaciens Streptococcus anginosus Pseudorhodobacter wandonensis Herpetosiphon aurantiacus Angiococcus disciformis Cylindrospermum licheniforme Shinella fusca Aeromonas sobria Shewanella oneidensis Corynebacterium matruchotii Pectobacterium atrosepticum Pseudoalteromonas citrea Luteimonas terricola Gibbsiella greigii Lactococcus lactis Streptococcus parasanguinis Veillonella tobetsuensis Luteimonas lutimaris
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.011 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.261 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.024 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.004 0.031 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Microbacterium maritypicum Rouxiella chamberiensis Proteiniclasticum ruminis Hydrogenophilus thermoluteolus Halomonas variabilis Methylobacterium bullatum Kurthia massiliensis Acinetobacter seifertii Nostoc punctiforme Chryseobacterium gregarium Pseudomonas cedrina Pseudomonas cuatrocienegasens Anaerococcus murdochii Psychrobacter jeotgali Zhihengliuella alba Peptoniphilus grossensis Chryseobacterium zeae Lactobacillus paracasei Parachlamydia acanthamoebae Massilia lutea Aeromonas rivuli Pseudomonas psychrophila Janthinobacterium agaricidamno Myroides profundi Aeromonas sanarellii Serratia liquefaciens Obesumbacterium proteus Carnobacterium gallinarum Ewingella americana Vagococcus penaei Morganella psychrotolerans Aeromonas fluvialis Clostridium putrefaciens Pseudomonas abietaniphila Vagococcus salmoninarum Providencia alcalifaciens Pseudomonas coleopterorum Chryseobacterium carnipullorum Pseudomonas syringae group gen Pseudomonas entomophila Aeromonas schubertii Pseudomonas protegens Serratia marcescens Hafnia alvei Brochothrix campestris Pseudomonas chlororaphis Pseudomonas trivialis Aquisalimonas lutea
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.082 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 13.230 12.611 2.130 1.004 0.447 0.361 0.232 0.147 0.093 0.064 0.064 0.036 0.036 0.021 0.021 0.018 0.018 0.014 0.014 0.011 0.011 0.007 0.007 0.008 0.007 0.007 0.004 0.004
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.008 0.004 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.016 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.012 0.000 0.000 0.000 0.078 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.106 0.000 0.000 0.000 0.000 0.000 0.004 0.667 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.008 25.215 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.004 0.000 0.000 0.000 0.000 0.000
Spirillum winogradskyi Exiguobacterium himgiriensis Azotobacter salinestris Myroides gitamensis Pseudomonas cremoricolorata Thauera phenylacetica Pseudomonas plecoglossicida Flavobacterium hydatis Flavobacterium hercynium Pseudomonas zhaodongensis Pseudomonas graminis Melissococcus plutonius Pseudomonas salomonii Pseudochrobactrum kiredjianiae Bacillus nanhaiisediminis Photobacterium kishitanii Terrimonas rubra Ochrobactrum gallinifaecis Pseudomonas brassicacearum Carnobacterium alterfunditum Ancylobacter abiegnus Vibrio litoralis Pseudomonas xinjiangensis Marinobacter nitratireducens Myroides pelagicus Halothiobacillus neapolitanus Ichthyenterobacterium magnum Myroides xuanwuensis Azomonas macrocytogenes Hafnia paralvei Oceanospirillum beijerinckii Shewanella fodinae Balneatrix alpica Niveispirillum fermenti Methylophilus leisingeri Enterobacter cloacae Gallionella capsiferriformans Flavobacterium spartansii Rheinheimera texasensis Pseudomonas panacis Pseudomonas thivervalensis Halochromatium salexigens Luteibacter rhizovicinus Zoogloea ramigera Lactobacillus graminis Salmonella enterica Enterococcus diestrammenae Massilia lurida
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.016 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004
Myroides marinus Erwinia billingiae Aeromonas popoffii Cluster 805 Paracoccus limosus Siccibacter turicensis Cluster 633 Rivibacter subsaxonicus Hymenobacter tibetensis Agrobacterium tumefaciens Glutamicibacter mysorens Chryseobacterium hominis Novosphingobium chloroacetimid Cluster 7 Cluster 375 Cloacibacterium normanense Amnibacterium soli Cluster 799 Gemella parahaemolysans Galbitalea soli Methylobacterium trifolii Cluster 1406 Brevibacterium pityocampae Devosia limi Corynebacterium ureicelerivorans Paracoccus angustae Cluster 638 Yersinia aldovae Calothrix desertica Bacillus tequilensis Cluster 632 Chryseobacterium palustre Anaerococcus senegalensis Cluster 603 Chryseobacterium haifense Craurococcus roseus Rhizobium cellulosilyticum Comamonas denitrificans Leptotrichia buccalis Pseudomonas aeruginosa Porphyrobacter sanguineus Leucothrix pacifica Kytococcus sedentarius Pedobacter xixiisoli Corynebacterium appendicis Pseudomonas alcaligenes Streptococcus sinensis Sphingomonas ginsengisoli An et
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.228 0.169 0.145 0.145 0.110 0.110 0.106 0.090 0.086 0.078 0.075 0.071 0.067 0.055 0.055 0.051 0.051 0.051 0.051 0.047 0.043 0.039 0.039 0.035 0.035 0.036 0.035 0.035 0.035 0.031 0.031 0.024 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.016 0.016 0.016 0.016 0.016
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Cellulomonas flavigena Pantoea dispersa Nocardioides daejeonensis Rhizobium aegyptiacum Rudaeicoccus suwonensis Anaerococcus provenciensis Arthrobacter flavus Granulicatella adiacens Herbaspirillum aquaticum Adhaeribacter aquaticus Conchiformibius kuhniae Sphingobium ummariense Veillonella atypica Pseudoalteromonas translucida Methylobacterium jeotgali Sphingomonas kyungheensis Veillonella montpellierensis Erwinia iniecta Staphylococcus petrasii Acaricomes phytoseiuli Kineococcus xinjiangensis Bergeyella porcorum Parascardovia denticolens Pseudomonas duriflava Sphingomonas paucimobilis Vibrio scophthalmi Altererythrobacter marinus Georgenia halophila Bifidobacterium breve Nocardioides antarcticus Sagittula marina Facklamia tabacinasalis Phaselicystis flava Pelomonas aquatica Vibrio tasmaniensis Roseomonas terrae Devosia insulae Nitratireductor kimnyeongensis Streptococcus constellatus Aerococcus viridans Haemophilus haemolyticus Geodermatophilus africanus Sandaracinobacter sibiricus Capnocytophaga ochracea Blastochloris viridis Pseudomonas fuscovaginae Leptotrichia hongkongensis Shewanella vesiculosa
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.016 0.016 0.016 0.016 0.016 0.016 0.016 0.016 0.016 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008
0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Sphingopyxis contaminans Thermus composti Clostridium clostridioforme Flavobacterium cheniae Pseudomonas straminea Flavisolibacter ginsengisoli Geobacter metallireducens Pseudomonas mediterranea Gemmobacter tilapiae Stenotrophomonas maltophilia Chryseobacterium jejuense Rhizobacter fulvus Kastovskya adunca Arthrobacter livingstonensis Pseudomonas taetrolens Corynebacterium jeikeium Pasteurella canis Thioprofundum lithotrophicum Arcobacter suis Microbacterium awajiense Rothia nasimurium Filifactor alocis Alcanivorax pacificus Staphylococcus cohnii Hyphomicrobium chloromethanic Arthrobacter russicus Acinetobacter variabilis Balneola alkaliphila Massilia varians Lewinella nigricans Deinococcus xinjiangensis Marinomonas polaris Actinomyces graevenitzii Hoeflea halophila Enterococcus alcedinis Psychrobacter aestuarii Halospirulina tapeticola Staphylococcus chromogenes Xanthomonas campestris Coprococcus catus Brevibacterium permense Enterococcus asini Modestobacter multiseptatus Sphingomonas cynarae Escherichia coli Anaerobacillus macyae Corynebacterium sputi Rubellimicrobium mesophilum
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.008 0.008 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Mycetocola lacteus Bacteroides vulgatus Comamonas nitrativorans Jannaschia faecimaris Chryseobacterium pallidum Rhodobacter blasticus Corynebacterium pilbarense Micrococcus flavus Rhizobium rosettiformans Flavobacterium aquatile Labrys wisconsinensis Dialister propionicifaciens Acidipropionibacterium jensenii Novosphingobium gossypii Pontibacter populi Flavobacterium terrae Vibrio cyclitrophicus Chryseobacterium xinjiangense Weeksella virosa Shewanella irciniae Serratia nematodiphila Pseudonocardia yuanmonensis Micrococcus lylae Halomonas songnenensis Azotobacter chroococcum Aliiroseovarius halocynthiae Nitrosococcus watsonii Capnocytophaga gingivalis Mariniflexile ostreae Peptoniphilus harei Erwinia tasmaniensis Peptoniphilus stercorisuis Janibacter cremeus Colwellia asteriadis Nitrospira moscoviensis Pseudonocardia ailaonensis Kineosporia rhizophila Microbacterium mitrae Nocardioides sediminis Taibaiella koreensis Actinomyces israelii Desemzia incerta Brevibacterium senegalense Flavobacterium rivuli Erwinia toletana Eubacterium desmolans Domibacillus indicus Tomitella biformata
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Rhizobacter gummiphilus Thauera chlorobenzoica Acidibacter ferrireducens Flavobacterium frigoris Alkalibacter saccharofermentans Dolosicoccus paucivorans Nocardioides solisilvae Celeribacter baekdonensis Rhodococcus triatomae Brevibacterium daeguense Dokdonella fugitiva Flavobacterium limicola Herbaspirillum chlorophenolicum Rhodococcus kyotonensis Ohtaekwangia koreensis Flavobacterium piscis Hydrocarboniphaga daqingensis Caloramator proteoclasticus Staphylococcus capitis Flavobacterium marinum Lysinibacter cavernae Methyloversatilis discipulorum Ferruginibacter alkalilentus Pedobacter aquatilis Brachybacterium fresconis Silanimonas mangrovi Lysobacter niastensis Marinobacter mobilis Sphingobacterium alimentarium Pandoraea pulmonicola Pseudomonas palleroniana Pectobacterium cacticida Methylobacillus glycogenes Marinobacter lutaoensis Methylocystis bryophila Methylobacillus flagellatus Modicisalibacter tunisiensis Massilia kyonggiensis Thalassomonas haliotis Azovibrio restrictus Selenomonas artemidis Intestinimonas butyriciproducens Zoogloea caeni Tolumonas auensis Flavobacterium weaverense Shannon Value CHAO1 Richness Estimation
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 3.445 675
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 2.884 532
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 3.868 722
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 3.190 684
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.903 401
ets (including those present at <1% of the global microbiome). y. .
Table 6‐ Sequencing statistics for the fungal ITS microbiome libraries prepared from control and The number of Number of raw, paired‐end and filtered reads, average length (in base pairs, bp and average quality (Phred Q value) are shown for each sea bass muscle microbiome library. Library C11 HP67 Total Average
Raw reads Paired‐end reads Filtered reads Av. Length Total Mb Av. Qual. 78186 36491 34520 387 13.4 36.7 116874 29029 28904 278 8.0 38.5 195060 65520 63424 665 21.4 75.2 97530 32760 31712 332 10.7 37.6
d HPP sea bass fillets at the end of storage. )
Table 7‐ Main fungal genera detected by ITS sequencing, at >1% of all the genera detected per libra The rela ve propor on of a given genera rela ve to the total reads iden fied in each library is shown as a pe Only genera detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion).
Main genus Saccharomyces Malassezia Cordyceps Candida Aspergillus Rhodotorula Kurtzmaniella Cladosporium Penicillium Urtica Verrucaria Cluster 46 Metschnikowia
C11 96.842 0.539 0.316 0.226 0.153 0.139 0.090 0.076 0.073 0.014 0.000 0.000 0.000
HP67 0.332 4.013 8.923 51.253 3.217 1.923 2.058 7.154 2.458 1.169 1.623 1.623 1.494
ary. rcentage.
Table 8- All fungal genera detected by ITS sequencing, including genera represented at low propor o Values represent the relative proportion of each genus relative to the total reads identified in each library. All detected genera are listed but those at < 1% of the total metagenome in each library are shaded in grey.
Main genus C11 HP67 Saccharomyces 96.842 0.332 Malassezia 0.539 4.013 Cordyceps 0.316 8.923 Candida 0.226 51.253 Cluster 130 0.223 0.000 Mortierella 0.156 0.000 Aspergillus 0.153 3.217 Rhodotorula 0.139 1.923 Peniophora 0.116 0.000 Didymella 0.098 0.000 Kurtzmaniella 0.090 2.058 Itersonilia 0.084 0.000 Phanerochaete 0.078 0.249 Cladosporium 0.076 7.154 Pichia 0.076 0.585 Penicillium 0.073 2.458 No Hit 0.055 0.325 Schizophyllum 0.055 0.000 Theobroma 0.046 0.000 Antrodia 0.038 0.367 Debaryomyces 0.038 0.000 Cutaneotrichospor 0.032 0.518 Parastagonospora 0.032 0.235 Phlebia 0.032 0.000 Ramularia 0.032 0.000 Sistotremastrum 0.026 0.931 Alternaria 0.026 0.010 Cluster 172 0.023 0.000 Meyerozyma 0.023 0.000 Platanus 0.020 0.339 Carex 0.020 0.000 Diplosphaera 0.017 0.000 Urtica 0.014 1.169 Aegopodium 0.014 0.000 Fragaria 0.014 0.000 Salvia 0.012 0.318 Fusarium 0.012 0.104 Galactomyces 0.012 0.000 Candida 0.012 0.000 Lecania 0.009 0.000 Sporidiobolus 0.009 0.000 Stellaria 0.009 0.000 Juglans 0.009 0.000
Yarrowia Wallemia Linum Spinacia Diplotaxis Petroselinum Cannabis Bacillus Pistacia Triticum [0]Eukaryota;[1]nu Trichoderma Saitozyma [0]Eukaryota;[1]As Zygosaccharomyce Lactobacillus Cryptococcus Achillea Verrucaria Cluster 46 Metschnikowia Cluster 77 Lophodermium Cluster 52 [0]Eukaryota;[1]As Rhizopus Cluster 154 Myrmecridium Cystobasidium Vishniacozyma Spegazzinia Entoloma Coprinopsis Cluster 121 Blumeria Gloeocystidiellum Cluster 70 Burgoa Brassica Lepista Vibrissea Verrucocladospori Vaginatispora Cluster 78 Plantago [0]Eukaryota;[1]Ba Mercurialis Deconica
0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.297 0.280 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.359 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.000 1.623 1.623 1.494 0.848 0.443 0.381 0.336 0.329 0.329 0.329 0.297 0.284 0.239 0.235 0.235 0.225 0.221 0.218 0.211 0.208 0.183 0.176 0.173 0.173 0.170 0.163 0.163 0.163 0.159 0.128
Aleurodiscus 0.000 Trebouxia 0.000 Fuscoporia 0.000 Cluster 43 0.000 Elymus 0.000 Cluster 48 0.000 Prunus 0.000 Cluster 5 0.000 Cynodon 0.000 Lecanicillium 0.000 Cystofilobasidium 0.000 Cluster 28 0.000 Cluster 30 0.000 Fraxinus 0.000 Polyporus 0.000 Kazachstania 0.000 Sclerotinia 0.000 Rhizoctonia 0.000 Lactococcus 0.000 Grosmannia 0.000 Neottiosporina 0.000 Lophiostoma 0.000 Shannon Value 0.24319
0.114 0.107 0.104 0.090 0.055 0.052 0.048 0.038 0.034 0.034 0.028 0.024 0.024 0.021 0.014 0.003 0.003 0.003 0.003 0.003 0.003 0.003 2.2408
ons (<1% of all the genera detected) per library.
Table 9‐ Main fungal species detected by ITS sequencing, at >1% of all the species detected p The rela ve propor on of a given species rela ve to the total reads iden fied in each library is shown Only species detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the metagenome they are shaded from (low proportion) to red (high proportion).
Main species Saccharomyces cerevisiae Malassezia restricta Cordyceps confragosa Candida hollandica Aspergillus vitricola Candida zeylanoides Cladosporium sp. Candida sp. WSYC 780 Penicillium sp. NX‐deepsea Rhodotorula mucilaginosa Cluster 46 Verrucaria csernaensis Metschnikowia bicuspidata Urtica membranacea
C11 96.749 0.443 0.316 0.165 0.136 0.09 0.076 0 0 0 0 0 0 0
HP67 0.332 3.186 8.923 2.038 2.778 2.058 7.151 48.841 1.847 1.667 1.623 1.623 1.491 1.169
per library. as a percentage. m grey
Figure 1 – Alpha rarefaction 16S
Figure 2 – Alpha rarefaction ITS
Figure 3 – Venn diagram of proteins with changed levels Myofibrillar HP1vsC1
HP67vsC11
11
Sarcoplasmic HP67vsC11
HP1vsC1
15 4
37
176
49
CONFLICT of INTEREST STATEMENT
I declare on behalf of all the authors that they have no conflict of interest. •
None of the authors have financial/personal interests or belief that may affect their objectivity;
•
None of the authors have competing interests as a result of personal or academic relationships;
•
The MS has not been submitted to another journal and will not be submitted in the future should it be accepted by DiB. Yours sincerely Deborah M Power Corresponding author 07 June 2019
Universidade do Algarve - Campus de Gambelas - 8000-810 - FARO - Portugal + 351 289 800 900 + 351 289 818 353 [email protected]://www.ualg.pt/ccmar
S2352-3409(19)30806-6
DOI:
https://doi.org/10.1016/j.dib.2019.104451
Reference:
DIB 104451
To appear in:
Data in Brief
Received Date: 17 May 2019 Revised Date:
7 June 2019
Accepted Date: 21 August 2019
Please cite this article as: L. Anjos, P.I.S. Pinto, T. Tsironi, G. Dimopoulos, S. Santos, C. Santa, B. Manadas, A. Canario, P. Taoukis, D.M. Power, Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets, Data in Brief, https://doi.org/10.1016/j.dib.2019.104451. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Published by Elsevier Inc.
MetaMeta-Data
*Title:
Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus (Dicentrarchus labrax) labrax) fillets
*Authors:
Liliana Anjos1,*, Patricia I. S. Pinto1,*, Theofania Tsironi2, George Dimopoulos2, Soraia Santos1, Cátia Santa3,4, Bruno Manadas3, Adelino Canario1, Petros Taoukis2,#, Deborah M. Power1,#
*Affiliations:
1
Comparative Endocrinology and Integrative Biology
Group (CEIB), Centro de Ciencias do Mar (CCMAR), University of Algarve, Faro, Portugal 2
Laboratory of Food Chemistry and Technology,
School of Chemical Engineering, National Technical University of Athens (NTUA), Greece 3
Center for Neuroscience and Cell Biology (CNC),
University of Coimbra, Portugal 4
Institute for Interdisciplinary Research, University
of Coimbra (IIIUC) * Equal contribution #-corresponding authors *Contact email:
Deborah M. Power, [email protected]; Petros Taoukis, [email protected]
*Co CoCo-authors: authors
Liliana Anjos, [email protected]; Patricia I. S. Pinto, [email protected]; Theofania Tsironi, [email protected]; George Dimopoulos, [email protected]; Soraia Santos, [email protected]; Cátia Santa, [email protected]; Bruno Manadas, [email protected]; Adelino Canario, [email protected].
*CATEGORY:
Biochemistry; Food science; Molecular Biology
Data Article
Title: Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus (Dicentrarchus labrax) labrax) fillets Authors: Liliana Anjos1,*, Patricia I. S. Pinto1,*, Theofania Tsironi2, George Dimopoulos2, Soraia Santos1, Cátia Santa3,4, Bruno Manadas3, Adelino Canario1, Petros Taoukis2,#, Deborah M. Power1,# Affiliations: 1 Comparative Endocrinology and Integrative Biology Group (CEIB), Centro de Ciencias do Mar (CCMAR), University of Algarve, Faro, Portugal 2
Laboratory of Food Chemistry and Technology, School of Chemical Engineering,
National Technical University of Athens (NTUA), Greece 3
Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Portugal
4
Institute for Interdisciplinary Research, University of Coimbra (IIIUC)
* These authors contributed equally for this work #Corresponding authors. Postal address: Deborah Power, Centro de Ciencias do Mar (CCMAR), University of Algarve, Campus de Gambelas, Faro, Portugal; Tel: +351800100; E-mail: [email protected] Petros Taoukis, Iroon Polytechniou, Zografou 15780, Athens, Greece; Tel: +302107723171; E-mail: [email protected] Short title: Data from high pressure processing and quality evaluation of fish fillets Contact email: email [email protected], [email protected] Abstract Fresh fish are highly perishable food products and their short shelf-life limits their commercial exploitation and leads to waste, which has a negative impact on aquaculture sustainability. New non-thermal food processing methods, such as high pressure (HP) processing, prolong shelf-life while assuring high food quality. The effect of HP processing (600MPa, 25ºC, 5min) on European sea bass (Dicentrarchus labrax) fillet quality and shelf life was investigated. The data presented comprises microbiome and proteome profiles of control and HP-processed sea bass fillets from 1 to 67 days of isothermal storage at 2°C. Bacterial diversity was analysed by Illumina high-throughput sequencing of the 16S rRNA gene in pooled DNAs from control or HP-processed fillets after 1, 11 or 67 days and the raw reads were deposited in the NCBI-SRA database with
accession number PRJNA517618. Yeast and fungi diversity were analysed by highthroughput sequencing of the internal transcribed spacer (ITS) region for control and HP-processed fillets at the end of storage (11 or 67 days, respectively) and have the SRA accession number PRJNA517779. Quantitative label-free proteomics profiles were analysed by SWATH-MS (Sequential Windowed data independent Acquisition of the Total High-resolution-Mass Spectra) in myofibrillar or sarcoplasmic enriched protein extracts pooled for control or HP-processed filets after 1, 11 and 67 days of storage. Proteome data was deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD012737. These data support the findings reported in the associated manuscript “High pressure processing of European sea bass (Dicentrarchus labrax) fillets and tools for flesh quality and shelf life monitoring”,
Tsironi
et
al.
2019,
JFE
262:83-91,
doi.org/10.1016/j.jfoodeng.2019.05.010.
Keywords: Colour and texture; European sea bass; fish fillet; high pressure; 16S rRNA microbiome; quantitative proteomics
Specifications Table Subject area
Food science
More specific subject
Food processing, Aquaculture
area Type of data
Tables, figures, supplementary tables
How data was
CR-Minolta chromameter, Stable Micro Systems TA-XT2i texture
acquired
analyzer, Illumina MiSeq, ABSciex Triple TOF 5600 LC-SWATHMS system with information-dependent acquisition (IDA)
Data format
Raw, metadata
Experimental factors
Marine cultured sea bass were refrigerated on ice, industrially scaled, the head removed, filleted and submitted to high pressure or not (control) and then isothermally stored (2ºC) for different periods.
Experimental
Colour and texture measurements; DNA extraction, quality
features
evaluation, microbiome library construction of 16S rRNA and internal transcribed spacer PCR amplified regions, sequencing and bioinformatics analysis; protein extractions, quality and quantity estimation, LC-SWATH-MS identification and quantification.
Data source location
Koropi, Attica, Greece (37°53'12.4"N; 23°52'40.4"E)
Data accessibility
Data is available in this article and at https://www.ncbi.nlm.nih.gov/sra/ (projects PRJNA517618 and PRJNA517779) and at the ProteomeXchange Consortium via the PRIDE repository with identifier PXD012737.
Related research
Theofania Tsironi, Liliana Anjos, Patricia I. S. Pinto, George
article
Dimopoulos, Soraia Santos, Cátia Santa, Bruno Manadas, Adelino Canario, Petros Taoukis, Deborah Power “High pressure processing of European sea bass (Dicentrarchus labrax) fillets and tools for flesh quality and shelf life monitoring”. Journal of Food Engineering 262, 83 – 91 https://doi.org/10.1016/j.jfoodeng.2019.05.010
Value of the Data •
The data represents an integrated view of the impact of novel non-thermal processing technologies on fish fillet quality and safety and makes a relevant contribution to the food loss and waste paradigm.
•
A metagenomics approach for the microbiome used 16s rRNA (bacteria) and internal transcribed spacer (for yeast and fungi) sequencing to provide a global and in-depth overview of microorganism diversity and dynamics in control and HP processed fillets during storage at 2ºC.
•
SWATH-MS proteomics is used to give insight for the first time into the global impact of HP processing and cold storage on the proteins in sea bass fillets.
•
The study demonstrates how integration of conventional experimental methods and novel omics technologies corroborate and substantially extend the outcome of previous work.
•
HP processing is revealed as an emerging processing approach that prolongs the shelf life of highly perishable commodities such as fish fillets.
Data Table 1 summarizes 16S rRNA sequencing quality statistics of HP-treated and control fillets at different time-points during storage, and Figure 1 presents the alpha rarefaction analysis of the same data, showing that all sequencing libraries were near to saturation. Table 2 lists the main bacterial genera detected and their relative abundance in each sequencing library. Table 3 lists all bacterial genera detected including those present in low abundance (<1% of the total genera detected). Similarly, Tables 4 and 5 contain, respectively, lists of the most abundant bacteria species and complete list of detected bacteria, together with the Shannon diversity index and CHAO1 Richness Estimation for each library. Table 6 summarizes the ITS sequence quality statistics for yeast and fungi of HP-treated and control fillets at the end of storage and Figure 2 presents the alpha rarefaction analysis of the same data, showing that all sequencing libraries were near to saturation. Tables 7 and 9 list, respectively, the main fungal genera and species detected by ITS in 1% or more of the reads. Tables 8 and 10 list, respectively, the full list of fungal genera and species detected by ITS. Table 11 summarizes the number of proteins and peptides from myofibrillar and sarcoplasmicenriched protein extracts that were identified or quantified by SWATH analysis in HPprocessed or control fillets at different storage times. Tables 12 and 13 (spreadsheet format) list the quantification parameters of the total proteome profiles of myofibrillar and sarcoplasmic extracts, respectively. Table 14 lists the 38 proteins from the myofibrillar extract and Table 15 lists the 263 proteins from the sarcoplasmic extract (in spreadsheet format) that changed greater than 2-fold between the control and HPprocessed fillets and/or between cold storage times; the relative proportions of the proteins are summarized in Figure 3. Experimental Design, Materials, and Methods Experimental setset-up and fish fillet samples European sea bass (Dicentrarchus labrax) (weight: 110 ± 10 g) from Nireus Aquaculture, were stunned on ice slush (0°C) immediately upon harvesting, size sorted and transported to the filleting line within 1 day. In the industrial facilities the fish was filleted after descaling and discarding of the head. Sea bass fillets were rinsed in tap
water, placed in polystyrene boxes with appropriate quantities of flaked ice (0°C) and transported to the Laboratory of Food Chemistry and Technology (NTUA, Athens, Greece) within 2-3 hours. A laboratory pilot scale Food Pressure Unit (FPU 1.01, Resato International BV, Roden, Holland) with a maximum operating pressure of 1000 MPa was used for high pressure treatments. The high-pressure unit had a 1.5 L volume and a multivessel system consisting of six vessels of 45 mL capacity each. All high-pressure vessels were surrounded by a circulating jacket connected to a heating-cooling system. The pressure transmitting fluid was polyglycol ISO viscosity class VG 15 (Resato International, BV, Roden, Holland). Fish fillets were packed in pouches (two per pouch) consisting of a multilayer (PP-PE) packaging material. HP processed fillets were vacuum-packed and treated in-pack at 600 MPa and 25°C for 5 min, according to [1]. The non-processed (control) samples were stored aerobically in non-sealed pouches, simulating conventional aerobic retail display facilities. Control and HP-treated (HP) fillets were stored at 2ºC under controlled isothermal conditions in high-precision (±0.2 °C) low-temperature incubators (Sanyo MIR 153, Sanyo Electric, Ora-Gun, Gunma, Japan), monitored with electronic, programmable miniature data-loggers (COX TRACER ®, Belmont, NC). Samples of ca 5 cm2 section were collected from fillets at different storage times: 1 day (C1 and HP1 groups), 11 days (C11 and HP11), 32 days (HP32) and 67 days (67HP). Samples from control fillets were not collected for the last two time points since they had deteriorated to unacceptable levels after 11 days of storage.
Colour and texture parameters Fillet colour change over time and treatment was measured using a CR-Minolta Chromameter (Minolta Co., Chuo-Ku, Osaka, Japan) with an 8mm diameter measuring area. The instrument was standardized under “C” illuminant conditions according to the CIE (Commission International de l’ Eclairage) using a standard white reference tile (calibration plate CR-200, L=97.50, a=-0.31, b=-3.83). Measurements of CIELab values (L-value: lightness, a-value: redness and greenness, b-value: yellowness and blueness) were made in three different points of the flesh on the upper side of the fillet (lateral line) and 3 fillets were tested per group and sampling point. Texture parameters were evaluated using a texture analyser with a load cell of 5 kg (TAXT2i, Stable Micro Systems, Godalming, Surrey, United Kingdom). A compression
aluminium plate of 75 mm or 20 mm diameter was selected. Double compression was applied to construct the texture profile analysis (TPA) parameters of 3 different fillets per group and sampling point. The aluminium plate approached the sample at the speed of 0.5 mm/sec and pressed 2 mm into the fish flesh. Then the force was reduced and the sample was allowed to rebound 5 s before the second compression. Force-time curves were obtained and texture parameters (hardness, cohesiveness and adhesiveness) were determined using Texture Expert Exceed Application (Version 2.64, Stable Micro Systems Ltd) [2].
DNA extracts Total DNA was separately extracted from 4 fillets from each of the groups C1, C11, HP1, HP11 and HP67, using a DNeasy Blood & Tissue Kit (Qiagen) with modifications. The excision of approx. 40 mg taken from the fillet surface was carried out under sterile conditions. Muscle samples were cut into small pieces, added to 2 ml sterile tubes with 200 µl of lysis buffer (20Mm Tris-HCl, pH 8; 2mM sodium EDTA; 1.2% Triton X-100; 40 mg/ml Lysozyme), 200 µl of AL buffer and approx. 400 mg of glass beads (0.1mm zirconia/silica beads, Biospec). The tubes were maintained on ice until mechanical disruption was carried out at room temperature in a Bertin Precellys 24 homogeniser; the disruption procedure was repeated in two pulses of 15 s at 6800 rpm. Tubes were incubated for 30 min at 37 ºC to complete the enzymatic lysis, and then 25 µl of proteinase K (supplied with the kit) was added to each tube, which were incubated for a further 30 min at 56 ºC. Tubes were centrifuged for 1 min at 8000 rpm and the lysed supernatant was recovered and incubated with RNAse A (10 µl of 10 mg/ml) for 10 min at room temperature. The remaining steps of the extraction method were performed as recommended in the protocol of the DNeasy Blood & Tissue Kit, with a final elution of extracted DNA in 50 µl of EB buffer (10Mm Tris-HCl, pH 8). DNA quality and integrity were analysed using a Nanodrop spectrophotometer and 1% agarose gel electrophoresis.
Microbiome library preparation, sequencing and bioinformatics analyses DNA pools were prepared from individual DNA extracts (ca. 250 ng each) and then concentrated with an RNeasy Micro Kit (Qiagen), following the manufacturer’s instructions, and eluted into 22 µl EB buffer. Five bacterial microbiome libraries (groups C1, C11, HP1, HP11 and HP67) were prepared from 75-210 ng of pooled DNA using the
16S Metagenomic Sequencing Library Preparation protocol (for the Illumina MiSeq system) and primers spanning the V3 and V4 regions of the 16S rRNA gene [3]. Two fungal microbiome libraries (C11 and HP67) were prepared from 30-130 ng of pooled DNA, using the same protocol as outlined above but with a set of primers directed against regions ITS3-ITS4 from Ascomycetes and Basidiomycetes [4]. Libraries were sequenced by Lifesequencing S.L.-ADM (Valencia, Spain) using an Illumina MiSeq instrument. Sequence data analysis, included: trimming of adaptors and filtering of lowquality reads that had a minimum value Q20 and minimum read length of 200 nucleotides; removal of chimeras and identification and classification of operational taxonomic units (OTU). Data analysis was carried out as described in [5]. For OTU assignments, sequence similarity searches were made against the NCBI 16S rRNA database (for the 16S libraries) or against an ITS built database of fungal ITS sequences extracted from NCBI (for the ITS libraries), with a cut-off set at 97% identity. Global microbiome data analyses were performed as described in [5], using a pipeline developed by Lifesequencing S.L.-ADM, and CD-HIT software [6] was used for hierarchy clustering.
Quantitative proteomics Differences in the protein content of control (C1, C11) and HP fillets (HP1, HP67) were analysed by SWATH-MS (Sequential Window data independent Acquisition of the Total High-resolution-Mass Spectra) using pools of myofibrillar or sarcoplasmic enriched protein extracts (n=6 per group). Myofibrillar and sarcoplasmic enriched protein extracts (based on [7] with modifications) were prepared from individual fillets (n=6 individuals per group). The extraction buffer used for the myofibrillar extracts was 50 mM Tris buffer pH 7.2, 100 mM DTT, 1.7% SDS and for sarcoplasmic extracts was 10 mM Tris buffer pH 7.2 and both buffers contained a protease inhibitor cocktail (Sigma, US). The muscle sample (0.5 g) was reduced to a powder in liquid nitrogen and ≈0.1 g was solubilized in extraction buffer (ratio: 1 g of tissue/8 ml of buffer), and mechanically disrupted using 0.5 mm zirconia/silica beads (Biospec) in a Bertin Precellys 24 homogeniser using 2 cycles of 20 s at 6,800 rpm. The homogenates were boiled at 95 ºC for 10 min and allowed to cool at room temperature. The soluble protein fraction was separated by centrifugation at 28,000 g for 15 min at 20 ºC, alkylated with 40 % acrylamide solution (1:15 of acrylamide: protein solution v/v) and stored at -80 ºC. The soluble protein concentration was determined using the Bradford assay with a bovine
serum albumin standard set (#500–0006 and #500–0207, BioRad, USA). The electrophoretic profile of the myofibrillar and sarcoplasmic enriched protein extracts was analysed by 1D SDS-PAGE (12%) according to [8] followed by Coomassie blue staining. For SWATH-MS proteomic analysis the protein extracts were gel digested using the short-GeLC method [9]. Briefly, a volume corresponding to 50 μg was prepared by pooling protein extracts from 6 individuals/treatment, and a further sample was prepared that consisted of 10 μg from each experimental group pool. The sample pools were fractionated by SDS- PAGE. Selected gel regions were excised and processed as in [10], for destaining, dehydration, rehydration, in-gel protein digestion using trypsin, peptide extraction and solid phase extraction with C18 sorbent (OMIX tip, Agilent Technologies). Samples were analysed using SWATH-MS on a Triple TOF™ 5600 System (Sciex®, Framingham, MA) using information-dependent acquisition (IDA) of the pooled mixture of all samples; followed by SWATH acquisition of each individual group pool. Peptide separation was performed using liquid chromatography (nanoLC Ultra 2D, Eksigent) on a ChromXP C18CL reverse phase column (300 µm × 15 cm, 3 µm, 120Å, Eksigent) at 5 µL/min with a 45 min gradient from 2% to 35% acetonitrile in 0.1% FA with 5% DMSO, and the peptides were eluted into the mass spectrometer using an electrospray ionization source (DuoSpray™ Source, Sciex). IDA experiments were performed by analysing each fraction of the pooled mixture. The mass spectrometer was set for IDA scanning full spectra (350-1250 m/z) for 250 ms, followed by up to 100 MS/MS scans (100–1500 m/z from a dynamic accumulation time – minimum 30 ms for precursors above the intensity threshold of 1000 counts per second (cps) – in order to maintain a cycle time of 3.3 s). Candidate ions with a charge state between +2 and +5 and counts above a minimum threshold of 10 cps were isolated for fragmentation and one MS/MS spectra was collected before adding those ions to the exclusion list for 25 s (mass spectrometer operated by Analyst® TF 1.7, Sciex). Rolling collision energy was used with a collision energy spread (CES) of 5. The SWATH setup was as in [11] with the same chromatographic conditions used for IDA acquisitions. Briefly, the mass spectrometer was operated in a looped product ion mode. The SWATH MS setup was designed specifically for the samples to be analysed, in order to adapt the SWATH windows to the complexity of this batch of samples. A set of 60 SWATH windows of variable width (containing 1 m/z for window overlap) was constructed covering the precursor mass range of 350-1250 m/z. A 200 ms survey scan
(350-1250 m/z) was acquired at the beginning of each cycle for instrument calibration and SWATH MS/MS spectra were collected from 100–1500 m/z for 50 ms resulting in a cycle time of 3.3 s from the precursors ranging from 350 to 1250 m/z. The collision energy for each window was determined according to the calculation for a charge +2 ion centered upon the window with variable CES (Collision Energy Spread) according to the window. Peptide identification and library generation were carried out using Protein Pilot software (v5.0, Sciex®) and the following search parameters: 1) comparison against the predicted proteins from the sea bass genome database (June 2012 draft assembly dicLab v1.0c with annotation from July 2013; file diclab1_pep.faa.gz downloaded from http://seabass.mpipz.mpg.de/DOWNLOADS/; [12]; 2) acrylamide alkylation; 3) trypsin digestion (ParagonTM Algorithm). The SWATHTM processing plug-in for PeakViewTM (v2.0.01, ABSciex®) was used for SWATH data processing, as described in [9] with minor modifications. Peptides were selected automatically from the library following the criteria: i) unique peptides for a specific targeted protein were ranked by the intensity of the precursor ion from the IDA analysis; ii) peptides that contained biological modifications and/or were shared between different protein entries/isoforms were excluded. Up to 15 peptides were chosen per protein, and SWATHTM quantification was attempted for all proteins considered as positive identifications. Peptide retention time was adjusted using the malE-GFP peptides. Up to 5 target fragment ions per peptide were automatically selected and the peak groups were scored following the criteria described in [13]. Protein levels were estimated by summing all the transitions from all the peptides for a given protein as described by [14] and normalized to the total intensity of the sample at the protein level. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [15] via the PRIDE [16] partner repository with the dataset identifier PXD012737. In proteomic data analysis, the count ratio method was used to compare the relative protein abundance modified by HP (HP1/C1; HP67/C11) or storage time (HP1/HP67; C1/C11) in each type of extract, considering 2-fold change as the threshold. Protein lists that changed with the experimental conditions were analysed using the proportional Venn diagram tool BioVenn (http://www.biovenn.nl) [17], UNIPROT (https://www.uniprot.org/) [18] and BRENDA (https://www.brenda-enzymes.org) [19] for screening of enzymes, structural proteins and other classifications. The zebrafish (Danio rerio) orthologues for the sea bass proteins were obtained using stand-alone
BlastX (with E value < 10-10) against the Ensembl zebrafish protein predictions (GRC Zebrafish
Build
10,
INSDC
Assembly
GCA_000002035.3
from
Sep
2014,
https://www.ensembl.org [20]) in order to establish a comparative annotation.
Acknowledgments This work was carried out under project “SUStainable production of HIgh quality aquaculture FISH using innovative tools and production strategies and integrating novel processing methods and cold chain management (Acronym: SUSHIFISH), co-funded by the COFASP ERA-NET partners (2nd call, 2016 - 2019), which received funding from the European Union’s seventh framework programme for research, technological development and demonstration under grant agreement no. 321553, together with Greek
national
funds
through
the
Operational
Program
"Competitiveness,
Entrepreneurship and Innovation 2014-2020" of the National Strategic Reference Framework (NSRF) and Portuguese national funds from FCT (Foundation of Science and Technology) through project COFASP/0002/2015. Support was also received from FCT through project UID/Multi/04326/2019, and from the operational programmes CRESC Algarve 2020 and COMPETE 2020 through project EMBRC.PT ALG-01-0145-FEDER022121, and through the European Marine Biological Research Infrastructure ClusterEMBRIC (EU H2020 research and innovation program, agreement nº 654008). The EMBRIC
configurator
data
management
service
(doi:10.7490/f1000research.
1116538.1) was used for advice on data coordination and standards. LA, PP and CS received
FCT
fellowships
SFRH/BPD/79105/2011,
SFRH/BPD/84033/2012
and
SFRH/BD/88419/2012, respectively. Authors contributions TT, PT and DMP conceived the study; AVMC, PT and DMP provided the resources; TT and GD performed the High-Pressure experiments, fish sampling and conventional microbiological, texture and colour analysis; Microbiome analysis of the fish fillets was performed by PISP and SS. LA, CS and BM carried out the protein analysis and SWATHproteomics and data analysis; TT, PT, DMP, AVMC, LA, PISP critically analysed and reviewed all the results of the study; TT, LA, PISP and DMP drafted the manuscript, which was critically reviewed and approved by all authors.
Figures and tables Figure legends Figure 11- Rarefaction plots for the microbiome libraries sequenced by 16S rRNA gene sequencing (bacterial diversity), determined based on all operational taxonomic units (OTUs) found at the genus level, including annotated and non-annotated sequences (no hits). Figure 22- Rarefaction plots for the microbiome libraries directed at the fungal ITS region, determined based on all operational taxonomic units (OTUs) found at the genus level, including annotated and non-annotated sequences (no hits). Figure 33- Venn diagrams representing the number of proteins that had a more than 2fold modified abundance in HPP sea bass fillets compared to the control groups (C) at the equivalent storage time in days (eg. short-term, C1 and HP1 or long-term, C11, HP67). Proteins were identified and quantified SWATH proteomics. For Myofibrillar (red) or sarcoplasmic (blue) protein extracts, the size of the circles is proportional to the number of proteins that had a modified expression (the number of proteins are indicated next to the circles). The proteins that changed in the same way between the different treatment groups at the beginning and end of storage are located in the intersection of two circles in the Venn diagram (the number of common proteins is underlined). Tables Table 11- Sequencing statistics for the 16S rRNA gene microbiome libraries sequenced in the study.
The number of Number of raw, paired end and filtered reads, average length (in base pairs, bp) and average quality (Phred Q value) are shown for each sea bass muscle microbiome library. Table 22- The main bacterial genera detected by 16s rRNA gene sequencing of control sea bass fillets or those subject to high-pressure (HP). The relative proportion of a given genera relative to the total reads identified in each library is shown as a percentage. Only genera detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion). Table 33- List of all the bacterial genera detected by 16S rRNA gene sequencing in sea bass fillets, including genera present at a low proportion (<1% of all the genera detected) per library. Values represent the relative proportion of each genus relative to the total reads identified in each library. All detected genera are listed but those at < 1% of the total microbiome in each library are shaded in grey. Table 44- The main bacterial species detected (>1% of all the species detected) by 16S rRNA gene sequencing. The relative proportion of a given species relative to the total reads identified in each library is shown as a percentage. Only species detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion). Table 55- All bacterial species detected by 16S rRNA gene sequencing in control or HPP sea bass fillets (including those present at <1% of the global microbiome). Values represent the relative proportion of each species relative to the total reads identified in each library. All detected species are listed but those at < 1% of the total microbiome in each library are shaded in grey.
Table 66- Sequencing statistics for the fungal ITS microbiome libraries prepared from control and HPP sea bass fillets at the end of storage. The number of Number of raw, paired end and filtered reads, average length (in base pairs, bp) and average quality (Phred Q value) are shown for each sea bass muscle microbiome library. Table 77- Main fungal genera detected by ITS sequencing, at >1% of all the genera detected per library. The relative proportion of a given genera relative to the total reads identified in each library is shown as a percentage. Only genera detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion). Table 88- All fungal genera detected by ITS sequencing, including genera represented at low proportions (<1% of all the genera detected) per library. Values represent the relative proportion of each genus relative to the total reads identified in each library. All detected genera are listed but those at < 1% of the total metagenome in each library are shaded in grey. Table 99- Main fungal species detected by ITS sequencing, at >1% of all the species detected per library. The relative proportion of a given species relative to the total reads identified in each library is shown as a percentage. Only species detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the metagenome they are shaded from grey (low proportion) to red (high proportion). Table 1010- All fungal species detected by ITS sequencing, including those represented at low proportions (<1% of all the species detected) per library. Values represent the relative proportion of each species relative to the total reads identified in each library. All detected species are listed but those at < 1% of the total fungal metagenome in each library are shaded in grey. Table 1111- Summary of the number of proteins and peptides identified or quantified in SWATH proteomic analysis.
Table 1212- Total proteome quantified by SWATH analysis for myofibrillar extracts. Table 1313- Total proteome quantified by SWATH analysis for sarcoplasmic extracts. Table 1414- Proteins identified by SWATH proteomics with a greater than a 2-fold change with HPP and/or storage time relative to the control in the myofibrillar extracts. Proteins whose levels decreased are shaded in green and whose levels increased in red. Table 1515- Proteins identified by SWATH proteomics with a greater than a 2-fold change with HPP and/or storage time in the sarcoplasmic extracts. Proteins whose levels decreased are shaded in green and whose levels increased in red.
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Table 1‐ Sequencing statistics for the 16S rRNA gene microbiome libraries sequenced in the study. The number of Number of raw, paired‐end and filtered reads, average length (in base pairs, bp) and average quality (Phred Q value) are shown for each sea bass muscle microbiome library.
Library C1 C11 HP1 HP11 HP67 Total Average
Raw reads Paired‐end reads Filtered reads Av. Length Total Mb Av. Qual. 65064 32116 29788 428 12.7 37.3 67298 33327 31625 431 13.6 37.3 53178 26176 23953 426 10.2 37.2 56300 27945 26364 429 11.3 37.2 55296 27443 26003 430 11.2 37.3 297136 147007 137733 59.0 59427 29401 27547 429 11.8 37.3
Table 10‐All fungal species detected by ITS sequencing, including those represented at low propo Values represent the relative proportion of each species relative to the total reads identified in ea All detected species are listed but those at < 1% of the total fungal metagenome in each library a Main species Saccharomyces cerevisiae Malassezia restricta Cordyceps confragosa Cluster 130 Candida hollandica Mortierella hyalina Aspergillus vitricola Rhodotorula sp. 1 CS‐2017 Peniophora cinerea Didymella arachidicola Malassezia globosa Saccharomyces sp. Candida zeylanoides Itersonilia perplexans Cladosporium sp. Phanerochaete chrysosporium Pichia fermentans Penicillium camemberti No Hit Schizophyllum commune Theobroma speciosum Antrodia sinuosa Debaryomyces hansenii Candida sp. CBS 10854 Cutaneotrichosporon curvatus Ramularia eucalypti Phlebia subserialis Parastagonospora nodorum Sistotremastrum guttuliferum Alternaria chlamydosporigena Meyerozyma guilliermondii Cluster 172 Platanus x hispanica Carex radiata Aspergillus pseudoglaucus Candida deformans Diplosphaera chodatii Aegopodium podagraria Fragaria virginiana Urtica dioica Rhodotorula glutinis Fusarium merismoides Galactomyces candidum
C11 96.749 0.443 0.316 0.223 0.165 0.156 0.136 0.127 0.116 0.098 0.096 0.093 0.090 0.084 0.076 0.075 0.073 0.061 0.055 0.055 0.046 0.038 0.038 0.035 0.032 0.032 0.032 0.032 0.026 0.026 0.023 0.023 0.020 0.020 0.017 0.017 0.017 0.014 0.014 0.014 0.012 0.012 0.012
HP67 0.332 3.186 8.923 0.000 2.038 0.000 2.778 0.000 0.000 0.000 0.796 0.000 2.058 0.000 7.151 0.000 0.585 0.000 0.325 0.000 0.000 0.367 0.000 0.000 0.393 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.339 0.000 0.125 0.000 0.000 0.000 0.000 0.000 0.256 0.104 0.000
Candida parapsilosis Candida sp. K2 Salvia coccinea Sporidiobolus metaroseus Juglans regia Stellaria media Lecania cyrtella Yarrowia lipolytica Penicillium olsonii Wallemia muriae Linum bienne Spinacia oleracea Pistacia lentiscus Diplotaxis tenuifolia Cannabis sativa Bacillus cytotoxicus Petroselinum crispum Triticum turgidum Salvia reuteriana fungal sp. suzu‐1 Saitozyma podzolica Trichoderma harzianum Cryptococcus sp. HB 1222 Pichia membranifaciens Lactobacillus letivazi Helotiales sp. EF830 Phanerochaete sp. JCM 28409 Penicillium robsamsonii Penicillium aeris Achillea wilsoniana Zygosaccharomyces lentus Candida sp. WSYC 780 Penicillium sp. NX‐deepsea Rhodotorula mucilaginosa Cluster 46 Verrucaria csernaensis Metschnikowia bicuspidata Urtica membranacea Sistotremastrum chilensis Cluster 77 Lophodermium sp. 3396 Cluster 52 Massarinaceae sp. 113 OG‐2012 Rhizopus oryzae Myrmecridium schulzeri Cluster 154 Cystobasidium slooffiae Vishniacozyma victoriae
0.012 0.009 0.009 0.009 0.009 0.009 0.009 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.374 0.000 0.000 0.000 0.000 0.000 0.294 0.287 0.138 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.359 0.318 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 48.841 1.847 1.667 1.623 1.623 1.491 1.169 0.931 0.848 0.443 0.381 0.336 0.329 0.329 0.329 0.291 0.284
Phanerochaete sp. BAB‐4915 Spegazzinia tessarthra Parastagonospora avenae Entoloma terreum Coprinopsis semitalis Cluster 121 Blumeria graminis Gloeocystidiellum kenyense Cluster 70 Burgoa anomala Penicillium citrinum Brassica napus Aspergillus penicillioides Lepista nuda Verrucocladosporium dirinae Vibrissea flavovirens Vaginatispora fuckelii Basidiomycota sp. NFLI 2000‐11 Cluster 78 Mercurialis canariensis Wallemia mellicola Aspergillus versicolor Deconica citrispora Cutaneotrichosporon moniliiform Penicillium pusillum Aleurodiscus thailandicus Trebouxia sp. 20 GS‐2016 Plantago coronopus Fuscoporia ferruginosa Cluster 43 Plantago lagopus Elymus tauri Cluster 48 Prunus kansuensis Cluster 5 Cynodon pascuus Malassezia obtusa Cystofilobasidium macerans Cluster 30 Lecanicillium muscarium Cluster 28 Fraxinus angustifolia Polyporus tuberaster Lecanicillium attenuatum Alternaria infectoria Neottiosporina paspali Kazachstania barnettii Yarrowia galli
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.249 0.239 0.235 0.235 0.235 0.225 0.221 0.218 0.211 0.208 0.204 0.183 0.183 0.176 0.173 0.173 0.170 0.163 0.163 0.159 0.142 0.131 0.128 0.125 0.114 0.114 0.107 0.104 0.104 0.090 0.059 0.055 0.052 0.048 0.038 0.031 0.031 0.028 0.024 0.024 0.024 0.021 0.014 0.010 0.007 0.003 0.003 0.003
Trichoderma lixii Penicillium rubens Sclerotinia sp. 5/97‐18 Cynodon dactylon Cladosporium sp. 2a_co2 Grosmannia olivacea Cystobasidium sp. Cystobasidium minutum Rhizoctonia sp. FA59209 Lactococcus lactis fungal sp. suzu‐11 Alternaria alternata Penicillium aurantiogriseum Lophiostoma macrostomum Metschnikowia aff. pulcherrima Shannon Value CHAO1 Richness Estimation
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.257 82
0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 2.423 178
or ons (<1% of all the species detected) per library. ach library. re shaded in grey.
Balance of the number of proteins and peptides in SWATH proteomic analysis of this study, for wh was obtained and to which were then applied quality filters (described in DIB methodology) to obt
Protein extract type
Myofibrillar Sarcoplasmic
Attempted Quantification (Identified)
Quantified Proteins Peptides
Quantified w Proteins
Proteins
Peptides
190
1359
180
948
178
337
1846
280
1231
277
hich the quantitative analysis was attempted, tain confident quantitative values.
with confidence Peptides 927 1216
,
Table 12 ‐ Worksheet Title Proteins_Itotal Proteins Peptides FDR Area ‐ ions Area ‐ proteins
Worksheet Description List of all the proteins quantified by SWATH with quantification after to normalization (quantitative values multiplied by 10,000) List of all the proteins quantified by SWATH before normalization List of all the peptides used for protein quantification with their respective intensity values FDR analysis of all the transitions used for protein quantification. List of all the transitions monitered with their respective intensity values List of all quantified proteins from SWATH plug‐in in peakview. The sum of the intensity of all proteins per sample is depicted and was used to normalyze the protein values in "Proteins_Itotal"
Table 13 ‐ Worksheet Title Proteins_Itotal Proteins Peptides FDR Area ‐ ions Area ‐ proteins
Worksheet Description List of all the quantified proteins with quantitative values after to normalyzation (quantitative values multiplied by 10.000) List of all the quantified proteins with quantitative values prior to normalyzation List of all the peptides used for protein quantification with their respective intensity values FDR analysis of all the transitions used for protein quantification. List of all the transitions monitered with their respective intensity values List of all quantified proteins from SWATH plug‐in in peakview. The sum of the intensity of all proteins per sample is depicted and was used to normalyze the protein values in "Proteins_Itotal"
Table 14 ‐ Worksheet Title 38 dif Balance of Protein numbers
Worksheet Description List of proteins whose levels changed more than 2‐fold with high‐pressure (HP) and/or storage time in myofibrillar extracts identified by SWATH General balance of the number of proteins in myofibrillar extracts
Table 15 ‐ Worksheet Title 263 dif Balance of Protein numbers
Worksheet Description List of proteins with a more than 2‐fold change in concentration with high‐ pressure (HP) and/or storage time in sarcoplasmic extract and identified by SWATH proteomics General balance of the number of proteins in sarcoplasmic extract
Table 2‐ The main bacterial genera detected by 16s rRNA gene sequencing of control sea bass fille The relative proportion of a given genera relative to the total reads identified in each library is shown as a Only genera detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion).
Genus Shewanella Acinetobacter Flavobacterium Pseudomonas Psychrobacter Chryseobacterium No Hit Aeromonas Rheinheimera Glutamicibacter Pseudoalteromonas Janthinobacterium Carnobacterium Myroides Paeniglutamicibacter Lactococcus Brochothrix Cluster 848
C1 41.3 16.0 13.7 7.7 5.6 2.7 1.3 0.9 0.9 0.4 0.4 0.3 0.1 0.1 0.0 0.0 0.0 0.0
C11 21.6 1.7 2.2 28.7 0.3 0.0 0.2 31.4 0.0 0.0 0.0 2.2 2.2 1.1 0.0 5.4 1.3 0.0
HP1 17.3 27.0 19.1 3.0 5.5 4.4 2.2 0.4 1.5 1.0 1.3 0.1 0.1 0.2 0.1 0.0 0.0 1.2
HP11 3.8 7.1 8.8 57.0 0.9 2.2 1.0 1.0 0.4 0.2 0.5 1.8 0.1 0.0 1.0 0.2 0.1 0.0
HP67 0.2 0.2 0.6 70.3 0.0 0.2 0.2 0.0 0.0 0.0 0.0 26.6 0.0 0.0 0.0 0.0 0.0 0.0
ets or those subject to high‐pressure (HP). percentage.
Table 3‐ List of all the bacterial genera detected by 16S rRNA gene sequencing in sea bass fillets including genera present at a low proportions (<1% of all the genera detected) per library. Values represent the relative proportion of each genus relative to the total reads identified in each libra All detected genera are listed but those at < 1% of the total microbiome in each library are shaded in gr
Genus Shewanella Acinetobacter Flavobacterium Pseudomonas Psychrobacter Chryseobacterium No Hit Aeromonas Rheinheimera Cluster 861 Epilithonimonas Comamonas Pseudoalteromonas Glutamicibacter Nocardia Stenotrophomonas Arthrobacter Pedobacter Janthinobacterium Massilia Corynebacterium Cutibacterium Streptococcus Brevundimonas Staphylococcus Pseudarthrobacter Cluster 22 Ralstonia Acidovorax Carnobacterium Cluster 1508 Sphingobacterium Paracoccus Variovorax Snodgrassella Riemerella Rothia Duganella Rhodoferax Neisseria Micrococcus Paraburkholderia
C1 41.286 16.004 13.741 7.717 5.569 2.747 1.295 0.940 0.930 0.761 0.534 0.501 0.354 0.353 0.330 0.319 0.301 0.280 0.252 0.231 0.200 0.182 0.182 0.172 0.171 0.171 0.167 0.167 0.163 0.148 0.134 0.122 0.112 0.111 0.089 0.085 0.082 0.082 0.078 0.074 0.074 0.074
C11 21.565 1.726 2.190 28.673 0.309 0.044 0.154 31.362 0.004 0.000 0.021 0.018 0.000 0.000 0.014 0.004 0.164 0.046 2.230 0.008 0.000 0.004 0.004 0.000 0.004 0.014 0.000 0.000 0.004 2.249 0.000 0.079 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000
HP1 17.294 27.023 19.082 3.000 5.522 4.429 2.196 0.423 1.503 0.000 0.187 0.611 1.343 1.044 0.379 0.410 0.325 0.338 0.085 0.088 0.560 0.727 0.455 0.213 0.424 0.308 0.000 0.491 0.240 0.107 0.000 0.160 0.673 0.000 0.183 0.330 0.178 0.013 0.076 0.151 0.254 0.325
HP11 3.773 7.113 8.846 57.041 0.923 2.202 0.989 1.025 0.385 0.000 0.231 0.110 0.531 0.165 0.118 0.216 0.361 0.236 1.753 0.008 0.606 0.514 0.522 0.177 0.659 0.981 0.000 0.024 0.028 0.082 0.000 0.051 0.228 0.012 0.122 0.055 0.185 0.004 0.031 0.141 0.436 0.000
HP67 0.185 0.181 0.624 70.338 0.008 0.233 0.181 0.032 0.008 0.000 0.004 0.004 0.004 0.048 0.685 0.000 0.125 0.016 26.586 0.012 0.000 0.000 0.076 0.004 0.004 0.024 0.000 0.000 0.008 0.012 0.000 0.028 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.004 0.000
Alkalibacterium Pseudochrobactrum Cluster 858 Myroides Cluster 44 Leucobacter Rhodanobacter Peptoniphilus Shinella Fluviicola Bacteroides Weissella Paeniglutamicibacter Cluster 990 Cedecea Haemophilus Cluster 734 Gelidibacter Diaphorobacter Cluster 477 Moraxella Devosia Trichococcus Lactobacillus Halomonas [0]Bacteria;[1]Proteoba Leptotrichia Cluster 196 Cluster 726 Lactococcus Polaromonas Cluster 811 Pseudoxanthomonas Bacteriovorax Lysobacter Cluster 891 Actinomyces Chishuiella Cluster 19 Cluster 738 Kocuria Dermacoccus Moheibacter Porphyromonas Xenophilus Roseomonas Pigmentiphaga Bosea
0.071 0.067 0.067 0.060 0.059 0.056 0.056 0.056 0.052 0.052 0.052 0.048 0.048 0.048 0.045 0.045 0.045 0.041 0.041 0.041 0.037 0.037 0.037 0.037 0.034 0.033 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.026 0.026 0.026 0.026 0.026 0.023 0.022 0.019 0.019 0.019 0.019 0.019
0.000 0.004 0.000 1.056 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.025 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.008 0.000 0.000 0.000 0.000 0.000 5.373 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.473 0.009 0.000 0.174 0.000 0.209 0.179 0.004 0.004 0.085 0.182 0.187 0.089 0.000 0.254 0.103 0.000 0.045 0.035 0.000 0.174 0.036 0.085 0.159 0.075 0.000 0.000 0.000 0.000 0.013 0.000 0.000 0.013 0.004 0.040 0.000 0.058 0.049 0.000 0.000 0.088 0.098 0.004 0.035 0.013 0.013 0.000 0.000
0.129 0.059 0.000 0.024 0.000 0.287 0.055 0.028 0.004 0.027 0.032 0.573 1.044 0.000 0.282 0.083 0.000 0.024 0.040 0.000 0.565 0.055 0.047 0.702 0.083 0.043 0.028 0.000 0.000 0.173 0.000 0.000 0.008 0.000 0.004 0.000 0.051 0.016 0.000 0.000 0.275 0.106 0.008 0.102 0.008 0.008 0.000 0.004
0.008 0.000 0.000 0.000 0.000 0.274 0.004 0.000 0.004 0.000 0.008 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.004 0.000 0.004 0.000 0.000 0.000 0.000
Luteimonas Gemella Leclercia Chondromyces Sphingomonas Lysinimonas Alcanivorax Alicycliphilus Rhodovulum Gemmobacter Fusobacterium Vagococcus Methylotenera Clostridium Hyphomicrobium Rhodococcus Prevotella Sanguibacter Pararhizobium Blastococcus Vibrio Facklamia Brucella Brochothrix Achromobacter Amaricoccus Bdellovibrio Alloprevotella Geothrix Bifidobacterium Veillonella Modestobacter Sinorhizobium Methylocystis Methylobacterium Bacillus Undibacterium Simplicispira Enterococcus Pantoea Capnocytophaga Dietzia Ramlibacter Brachybacterium Asaia Albirhodobacter Marinicella Finegoldia
0.018 0.018 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.014 0.014 0.012 0.012 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.007 0.007 0.007 0.007 0.007 0.007
0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.085 0.004 0.040 0.004 0.000 0.000 0.000 0.000 0.000 0.011 0.000 0.000 1.337 0.000 0.000 0.000 0.000 0.011 0.000 0.000 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.008 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.048 0.120 0.000 0.000 0.062 0.000 0.013 0.049 0.000 0.036 0.004 0.000 0.008 0.000 0.000 0.035 0.017 0.018 0.004 0.000 0.085 0.009 0.000 0.004 0.022 0.004 0.009 0.000 0.004 0.013 0.004 0.000 0.004 0.004 0.039 0.000 0.030 0.062 0.027 0.027 0.013 0.026 0.000 0.075 0.085 0.018 0.000 0.000
0.008 0.059 0.000 0.000 0.044 0.000 0.024 0.098 0.012 0.004 0.035 0.004 0.008 0.020 0.012 0.024 0.020 0.012 0.004 0.000 0.040 0.024 0.000 0.114 0.008 0.000 0.008 0.016 0.008 0.146 0.040 0.004 0.000 0.000 0.071 0.051 0.000 0.004 0.008 0.016 0.032 0.020 0.000 0.086 0.043 0.004 0.000 0.078
0.004 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000
Exiguobacterium Peredibacter Rosenbergiella Sulfuricella Erysipelothrix Granulicatella Methylopila Nitrosospira Luteolibacter Pseudorhodoferax Soonwooa Limnohabitans Escherichia Taibaiella Buttiauxella Spirosoma Rhizomicrobium Sphingopyxis Jiangella Thermomonas Pleomorphomonas Marinilactibacillus Nocardioides Paludibacterium Curvibacter Abiotrophia Chiayiivirga Deinococcus Leptonema Pelomonas Anaerococcus Methylophilus Proteiniphilum Streptomyces Hafnia Chitinophaga Myxococcus Planococcus Amphritea Thauera Morganella Colwellia Aliidiomarina Xylella Thermicanus Niastella Paraferrimonas Giesbergeria
0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.004 0.000 0.011 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.004 0.064 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.031 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.022 0.013 0.053 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.000 0.000 0.009 0.004 0.000 0.009 0.009 0.000 0.000 0.018 0.058 0.000 0.000 0.031 0.112 0.000 0.000 0.000 0.000 0.000 0.000 0.022 0.000 0.000 0.000 0.000 0.000 0.000
0.012 0.000 0.000 0.000 0.000 0.024 0.000 0.000 0.000 0.000 0.012 0.000 0.224 0.004 0.000 0.000 0.000 0.012 0.000 0.008 0.000 0.000 0.083 0.000 0.000 0.039 0.000 0.004 0.000 0.008 0.059 0.000 0.000 0.000 0.039 0.000 0.000 0.000 0.000 0.004 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Blastocatella Ahrensia Alcaligenes Phycicoccus Ochrobactrum Leptothrix Methylovirgula Elizabethkingia Sinibacillus Pseudopropionibacteriu Dolosigranulum Actibacter Hymenobacter Thioprofundum Kushneria Arcobacter Oxalicibacterium Rubellimicrobium Photobacterium Romboutsia Lautropia Bradyrhizobium Mesocricetibacter Zymobacter Cellulophaga Tetragenococcus Microbacterium Tahibacter Rubrivivax Rudaea Alkanindiges Leuconostoc Cluster 848 Cluster 25 Cluster 1415 Cluster 844 Cluster 975 Cluster 867 Knoellia Cluster 697 Cluster 650 Agreia Rhizobium Cluster 56 Pseudoclavibacter Cluster 793 Cluster 37 Cluster 27
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.013 0.000 0.004 0.116 0.000 0.018 0.036 0.000 0.000 0.027 0.000 0.009 0.000 0.000 0.022 0.000 0.013 0.018 0.000 0.009 0.004 0.000 0.000 0.000 0.000 0.030 0.000 0.009 0.009 0.017 0.004 1.174 0.362 0.335 0.214 0.094 0.094 0.089 0.085 0.076 0.067 0.058 0.058 0.054 0.054 0.049 0.049
0.000 0.000 0.000 0.035 0.000 0.000 0.000 0.016 0.000 0.000 0.008 0.000 0.110 0.004 0.000 0.004 0.000 0.043 0.008 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.047 0.000 0.000 0.008 0.024 0.016 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.040 0.000 0.012 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000
Cluster 439 Brevibacterium Cluster 785 Cluster 240 Cluster 57 Bavariicoccus Kluyvera Cluster 847 Cluster 611 Cluster 18 Serratia Nesterenkonia Ancylobacter Aquihabitans Peptoanaerobacter Delftia Kroppenstedtia Aeromicrobium Cardiobacterium Dyadobacter Geminicoccus Gemmatimonas Skermanella Marinomonas Negativicoccus Pseudorhodobacter Selenomonas Frondihabitans Mucilaginibacter Peptostreptococcus Salinicoccus Uruburuella Brachymonas Hydrogenophaga Altererythrobacter Actinokineospora Desulfovibrio Dysgonomonas Dermabacter Citrobacter Labilithrix Subsaxibacter Pseudonocardia Caulobacter Dialister Tetrasphaera Varibaculum Kineococcus
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.125 0.000 0.000 0.000 0.379 0.000 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.049 0.044 0.040 0.040 0.036 0.036 0.036 0.036 0.031 0.031 0.031 0.027 0.027 0.022 0.022 0.022 0.022 0.022 0.018 0.018 0.018 0.018 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.008 0.009 0.009 0.009 0.009 0.009 0.008 0.009 0.009 0.009 0.009 0.009 0.008 0.009 0.009
0.000 0.067 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.027 0.000 0.004 0.000 0.012 0.000 0.004 0.000 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.004 0.000 0.000 0.012
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Dokdonella Gordonia Gibbsiella Roseicitreum Bizionia Dongia Zhihengliuella Kurthia Parachlamydia Halodurantibacterium Rouxiella Mycobacterium [0]Bacteria;[1]Firmicute Cylindrospermum Polynucleobacter Hydrobacter Opitutus Cytophaga Cystobacter Hydrogenophilus Salinisphaera Rubritalea Novosphingobium Kingella Aridibacter Cloacibacterium Sediminibacterium Pectobacterium Herpetosiphon Euzebya Paludibaculum Cellulomonas Empedobacter Marmoricola Proteiniclasticum Nostoc Obesumbacterium Ewingella Providencia Melissococcus Salmonella Ichthyenterobacterium Terrimonas Marinobacter Gallionella Niveispirillum Halochromatium Azotobacter
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.082 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.232 0.093 0.018 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.009 0.009 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.008 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.082 0.000 0.000 0.067 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.004
0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.012
Aquisalimonas Enterobacter Balneatrix Erwinia Halothiobacillus Luteibacter Oceanospirillum Azomonas Spirillum Zoogloea Cluster 805 Cluster 633 Siccibacter Rivibacter Agrobacterium Cluster 7 Cluster 375 Cluster 799 Amnibacterium Galbitalea Cluster 1406 Cluster 638 Yersinia Cluster 632 Calothrix Cluster 603 Leucothrix Craurococcus Kytococcus Porphyrobacter Rudaeicoccus Herbaspirillum Adhaeribacter Sphingobium Acaricomes Conchiformibius Bergeyella Blastochloris Sagittula Georgenia Parascardovia Phaselicystis Sandaracinobacter Aerococcus Nitratireductor Geodermatophilus Thermus Rhizobacter
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.228 0.145 0.145 0.110 0.106 0.075 0.071 0.055 0.055 0.051 0.051 0.035 0.035 0.035 0.036 0.031 0.020 0.020 0.020 0.020 0.016 0.016 0.012 0.012 0.012 0.012 0.012 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008
0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Labrys Alkalibacter Anaerobacillus Coprococcus Acidibacter Nitrospira Celeribacter Mariniflexile Desemzia Geobacter Pontibacter Weeksella Xanthomonas Domibacillus Nitrosococcus Kastovskya Jannaschia Kineosporia Rhodobacter Lewinella Filifactor Halospirulina Eubacterium Mycetocola Janibacter Pasteurella Acidipropionibacterium Aliiroseovarius Lachnoclostridium [0]Bacteria;[1]Firmicute Balneola Tomitella Hoeflea Flavisolibacter Methylobacillus Methyloversatilis Azovibrio Intestinimonas Ohtaekwangia Silanimonas Lysinibacter Tolumonas Modicisalibacter Thalassomonas Pandoraea Hydrocarboniphaga Ferruginibacter Caloramator
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
s, ary. rey.
Table 4‐ The main bacterial species detected (>1% of all the species detected) by 16S rRNA ge The relative proportion of a given species relative to the total reads identified in each library is shown Only species detected at >1% of the global microbiome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from g Note that the HP treatment caused an approximately 3‐fold reduction in Shewanella baltica . In contrast, A. Johnsonii proportions were duplicated with HP treatments.
Main species Shewanella baltica Acinetobacter johnsonii Shewanella xiamenensis Shewanella putrefaciens Acinetobacter radioresistens Flavobacterium antarcticum Flavobacterium ummariense Flavobacterium qiangtangense Pseudomonas gessardii Flavobacterium dongtanense Pseudomonas asturiensis Psychrobacter pulmonis No Hit Shewanella denitrificans Chryseobacterium antarcticum Pseudomonas tolaasii Pseudomonas japonica Flavobacterium granuli Carnobacterium jeotgali Pseudomonas deceptionensis Shewanella profunda Acinetobacter junii Aeromonas salmonicida Lactococcus piscium Brochothrix thermosphacta Pseudomonas migulae Pseudomonas azotoformans Pseudomonas marginalis Aeromonas hydrophila Cluster 848 Janthinobacterium lividum Paeniglutamicibacter antarcticu Pseudomonas vranovensis Aeromonas rivuli Pseudomonas psychrophila Janthinobacterium agaricidamn Myroides profundi
C1 25.498 10.989 6.787 6.505 3.433 3.225 3.065 2.991 2.772 2.709 2.583 2.03 1.295 0.957 0.609 0.434 0.267 0.245 0.148 0.108 0.078 0.03 0.026 0.022 0.011 0.011 0.007 0.004 0.004 0 0 0 0 0 0 0 0
C11 15.26 0.143 2.652 1.698 1.122 0.011 0.014 0.032 8.693 0.004 0.018 0 0.154 0.011 0.007 0.1 2.324 2.059 2.098 0.064 1.255 0 1.101 5.298 1.33 1.223 0.021 1.437 15.31 0 0.05 0.025 0.004 13.23 12.611 2.13 1.004
HP1 9.709 22.05 2.089 2.232 1.848 2.29 5.236 2.116 0.004 6.539 0.004 2.468 2.196 2.504 1.299 0.018 0.241 0.192 0.094 0 0.004 1.214 0.138 0.009 0.004 0.004 1.004 0 0 1.174 0.085 0.085 0.004 0 0 0 0
HP11 1.989 5.39 0.635 0.431 0.608 1.585 2.224 1.236 35.156 2.052 0 0.016 0.989 0.6 0.647 0.51 0 0.18 0 1.483 0.012 0.008 0.934 0.173 0.114 0.012 16.754 0.024 0 0 1.71 1.04 1.043 0 0 0.004 0.012
HP67 0.101 0.141 0.004 0 0.024 0.06 0.214 0.085 0.028 0.137 0.004 0 0.181 0.032 0.097 70.021 0 0 0.012 0 0.04 0 0 0 0 0 0.012 0.032 0 0 0.895 0 0 0 0.008 25.215 0
ene sequencing. as a percentage. grey (low proportion) to red (high proportion).
Table 5‐ All bacterial species detected by 16S rRNA gene sequencing in control or HPP sea bass fill Values represent the relative proportion of each species relative to the total reads identified in each library All detected species are listed but those at < 1% of the total microbiome in each library are shaded in grey.
Main species Shewanella baltica Acinetobacter johnsonii Shewanella xiamenensis Shewanella putrefaciens Acinetobacter radioresistens Flavobacterium antarcticum Flavobacterium ummariense Flavobacterium qiangtangense Pseudomonas gessardii Flavobacterium dongtanense Pseudomonas asturiensis Psychrobacter pulmonis No Hit Shewanella denitrificans Aeromonas veronii Cluster 861 Psychrobacter arenosus Psychrobacter arcticus Shewanella hafniensis Psychrobacter piscatorii Flavobacterium frigidimaris Psychrobacter cryohalolentis Chryseobacterium antarcticum Chryseobacterium jeonii Epilithonimonas lactis Chryseobacterium balustinum Acinetobacter guillouiae Pseudomonas tolaasii Pseudomonas peli Acinetobacter kyonggiensis Chryseobacterium carnis Rheinheimera baltica Nocardia coeliaca Glutamicibacter arilaitensis Comamonas kerstersii Pseudomonas caeni Psychrobacter cibarius Flavobacterium gelidilacus Pseudoalteromonas haloplanktis Pseudomonas japonica Arthrobacter psychrochitiniphilus Rheinheimera aestuari Janthinobacterium svalbardensis
C1 25.498 10.989 6.787 6.505 3.433 3.225 3.065 2.991 2.772 2.709 2.583 2.030 1.295 0.957 0.798 0.761 0.742 0.716 0.693 0.672 0.635 0.616 0.609 0.568 0.527 0.512 0.482 0.434 0.430 0.427 0.412 0.371 0.330 0.327 0.308 0.286 0.278 0.275 0.275 0.267 0.260 0.252 0.252
C11 15.260 0.143 2.652 1.698 1.122 0.011 0.014 0.032 8.693 0.004 0.018 0.000 0.154 0.011 0.000 0.000 0.000 0.000 0.436 0.004 0.000 0.004 0.007 0.004 0.021 0.011 0.100 0.100 0.000 0.193 0.004 0.000 0.014 0.000 0.011 0.004 0.214 0.007 0.000 2.324 0.157 0.000 0.050
HP1 9.709 22.050 2.089 2.232 1.848 2.290 5.236 2.116 0.004 6.539 0.004 2.468 2.196 2.504 0.000 0.000 0.750 0.790 0.245 0.549 0.567 0.527 1.299 0.759 0.187 0.719 0.143 0.018 0.375 0.246 0.741 0.812 0.379 0.853 0.326 0.103 0.000 0.420 0.121 0.241 0.308 0.022 0.000
HP11 1.989 5.390 0.635 0.431 0.608 1.585 2.224 1.236 35.156 2.052 0.000 0.016 0.989 0.600 0.008 0.000 0.004 0.063 0.047 0.122 0.522 0.133 0.647 0.349 0.231 0.173 0.216 0.510 0.000 0.067 0.333 0.126 0.118 0.059 0.078 0.016 0.102 0.302 0.012 0.000 0.333 0.000 0.039
HP67 0.101 0.141 0.004 0.000 0.024 0.060 0.214 0.085 0.028 0.137 0.004 0.000 0.181 0.032 0.024 0.000 0.000 0.000 0.004 0.004 0.044 0.000 0.097 0.032 0.004 0.024 0.004 70.021 0.000 0.000 0.064 0.004 0.685 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.117 0.000 0.476
Flavobacterium granuli Shewanella glacialipiscicola Psychrobacter maritimus Pseudomonas flavescens Chryseobacterium yonginense Massilia aurea Pseudomonas helmanticensis Shewanella arctica Kim et al. 201 Cutibacteirum acnes Chryseobacterium lactis Stenotrophomonas terrae Pseudarthrobacter phenanthreni Cluster 22 Staphylococcus epidermidis Comamonas koreensis Acinetobacter lwoffii Carnobacterium jeotgali Rheinheimera soli Stenotrophomonas rhizophila Cluster 1508 Psychrobacter lutiphocae Acidovorax radicis Flavobacterium tegetincola Flavobacterium caeni Ralstonia pickettii Shewanella seohaensis Pseudomonas deceptionensis Corynebacterium kroppenstedtii Shewanella morhuae Acinetobacter baumannii Pseudomonas syringae Chryseobacterium polytrichastri Aeromonas aquatica Acinetobacter qingfengensis Streptococcus oralis Snodgrassella alvi Sphingobacterium anhuiense Riemerella columbipharyngis Rhodoferax ferrireducens Shewanella profunda Variovorax ginsengisoli Micrococcus aloeverae Rheinheimera aquimaris Flavobacterium oryzae Pseudoalteromonas prydzensis Rothia endophytica Pseudomonas fluorescens Pedobacter namyangjuensis
0.245 0.241 0.226 0.226 0.200 0.193 0.186 0.182 0.178 0.174 0.174 0.171 0.167 0.167 0.163 0.160 0.148 0.148 0.134 0.134 0.134 0.126 0.126 0.122 0.111 0.111 0.108 0.100 0.097 0.096 0.093 0.093 0.093 0.093 0.093 0.089 0.089 0.085 0.078 0.078 0.078 0.074 0.074 0.074 0.071 0.071 0.071 0.071
2.059 0.025 0.000 0.093 0.000 0.000 0.021 0.007 0.004 0.000 0.000 0.014 0.000 0.004 0.007 0.050 2.098 0.000 0.004 0.000 0.004 0.004 0.004 0.000 0.000 0.046 0.064 0.000 0.064 0.089 0.318 0.004 0.483 0.000 0.004 0.004 0.000 0.000 0.000 1.255 0.000 0.000 0.000 0.000 0.000 0.000 0.222 0.000
0.192 0.049 0.129 0.036 0.295 0.000 0.103 0.379 0.723 0.004 0.000 0.308 0.000 0.058 0.254 0.299 0.094 0.357 0.165 0.000 0.116 0.129 0.210 0.018 0.362 0.004 0.000 0.272 0.018 0.121 0.201 0.058 0.107 0.004 0.219 0.183 0.000 0.330 0.076 0.004 0.000 0.000 0.112 0.362 0.004 0.089 0.000 0.000
0.180 0.004 0.341 0.000 0.094 0.000 0.016 0.035 0.510 0.173 0.035 0.981 0.000 0.439 0.000 0.314 0.000 0.090 0.177 0.000 0.008 0.020 0.196 0.020 0.024 0.000 1.483 0.212 0.008 0.004 0.000 0.012 0.024 0.000 0.396 0.122 0.000 0.055 0.031 0.012 0.012 0.318 0.000 0.279 0.000 0.039 0.000 0.000
0.000 0.004 0.004 0.000 0.000 0.004 0.012 0.000 0.000 0.008 0.000 0.024 0.000 0.000 0.000 0.008 0.012 0.000 0.000 0.000 0.000 0.004 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.000 0.000 0.004 0.000 0.040 0.000 0.000 0.000 0.024 0.000 0.000 0.028 0.004
Brevundimonas bullata Pseudochrobactrum lubricantis Cluster 858 Alkalibacterium olivapovliticus Duganella phyllosphaerae Paraburkholderia caledonica Cluster 44 Paracoccus aminophilus Brevundimonas vesicularis Rhodanobacter glycinis Myroides phaeus Streptococcus parauberis Ralstonia syzygii Acinetobacter gerneri Bacteroides graminisolvens Shinella zoogloeoides Acinetobacter haemolyticus Fluviicola taffensis Shewanella basaltis Leucobacter salsicius Pedobacter ginsengiterrae Peptoniphilus gorbachii Chryseobacterium chaponense Flavobacterium akiainvivens Paeniglutamicibacter kerguelensi Cluster 990 Pedobacter nyackensis Flavobacterium anatoliense Neisseria mucosa Cluster 734 Paracoccus aminovorans Cedecea lapagei Cluster 477 Pseudomonas brenneri Weissella viridescens Rheinheimera chironomi Diaphorobacter aerolatus Acinetobacter beijerinckii Gelidibacter mesophilus Moraxella osloensis Corynebacterium accolens Trichococcus pasteurii Acidovorax soli Chryseobacterium indoltheticum Psychrobacter adeliensis Pseudomonas endophytica Variovorax soli Flavobacterium ponti
0.071 0.067 0.067 0.067 0.063 0.059 0.059 0.059 0.056 0.056 0.056 0.056 0.056 0.056 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.052 0.048 0.048 0.048 0.045 0.045 0.045 0.045 0.045 0.041 0.041 0.041 0.041 0.041 0.041 0.041 0.037 0.037 0.037 0.037 0.033 0.033 0.033 0.033 0.033
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.018 0.000 0.000 0.014 0.000 0.000 0.000 0.000 0.043 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.311 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.004 0.322 0.000 0.000
0.107 0.009 0.000 0.442 0.013 0.254 0.000 0.018 0.062 0.179 0.161 0.134 0.129 0.370 0.165 0.000 0.022 0.076 0.018 0.004 0.000 0.000 0.067 0.062 0.004 0.000 0.004 0.089 0.107 0.000 0.004 0.254 0.000 0.027 0.187 0.004 0.022 0.210 0.045 0.170 0.170 0.085 0.098 0.004 0.067 0.027 0.000 0.553
0.004 0.059 0.000 0.129 0.000 0.000 0.000 0.008 0.000 0.055 0.000 0.039 0.000 0.024 0.024 0.000 0.004 0.027 0.000 0.275 0.012 0.000 0.004 0.000 0.004 0.000 0.000 0.043 0.090 0.000 0.000 0.282 0.000 0.667 0.561 0.067 0.020 0.000 0.024 0.565 0.161 0.047 0.008 0.145 0.004 0.027 0.000 0.000
0.004 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.056 0.000 0.000 0.008 0.004 0.000 0.000 0.000 0.270 0.000 0.000 0.004 0.004 0.000 0.000 0.000 0.004 0.004 0.000 0.004 0.000 0.000 0.020 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.008 0.000 0.008
Shewanella amazonensis Devosia submarina Shewanella algae Acinetobacter schindleri Pseudomonas geniculata Cluster 726 Polaromonas naphthalenivorans Bacteriovorax stolpii Acinetobacter gandensis Psychrobacter aquaticus Acinetobacter junii Haemophilus parainfluenzae Pseudoxanthomonas yeongjuens Flavobacterium succinicans Acinetobacter bouvetii Cluster 891 Cluster 811 Lysobacter gummosus Leptotrichia wadei Cluster 196 Arthrobacter stackebrandtii Cluster 738 Glutamicibacter bergerei Chishuiella changwenlii Cluster 19 Pedobacter hartonius Pseudomonas guineae Aeromonas salmonicida Sphingobacterium nematocida Kocuria rhizophila Neisseria cinerea Lactococcus piscium Pseudomonas prosekii Streptococcus sanguinis Moheibacter sediminis Lactobacillus iners Chryseobacterium aahli Pedobacter steynii Actinomyces odontolyticus Psychrobacter alimentarius Xenophilus arseniciresistens Pigmentiphaga soli Dermacoccus abyssi Brevundimonas lenta Bosea robiniae Pedobacter koreensis Flavobacterium terrigena Halomonas lutea
0.033 0.033 0.033 0.033 0.033 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.030 0.026 0.026 0.026 0.026 0.026 0.026 0.026 0.026 0.026 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.019 0.019 0.019 0.019 0.019 0.019 0.019 0.019
0.004 0.000 0.014 0.000 0.000 0.000 0.000 0.000 0.000 0.036 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.000 0.000 0.000 0.000 0.000 0.004 1.101 0.000 0.000 0.000 5.298 0.097 0.000 0.000 0.000 0.000 0.046 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.036 0.004 0.040 0.000 0.000 0.000 0.004 0.080 0.009 1.214 0.085 0.000 0.031 0.027 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.183 0.049 0.000 0.000 0.009 0.138 0.058 0.000 0.000 0.009 0.009 0.049 0.004 0.134 0.031 0.089 0.009 0.004 0.013 0.000 0.000 0.022 0.000 0.112 0.013 0.027
0.000 0.000 0.000 0.012 0.043 0.000 0.000 0.000 0.192 0.000 0.008 0.051 0.008 0.027 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.008 0.016 0.000 0.000 0.078 0.934 0.012 0.012 0.000 0.173 0.008 0.008 0.008 0.698 0.008 0.118 0.008 0.008 0.008 0.000 0.000 0.075 0.004 0.090 0.012 0.055
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.048 0.000 0.000 0.000 0.004 0.000 0.012 0.000 0.000 0.000 0.020 0.000 0.000 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000
Massilia niastensis Pedobacter panaciterrae Porphyromonas pasteri Flavobacterium haoranii Leclercia adecarboxylata Acinetobacter puyangensis Massilia eurypsychrophila Gemmobacter intermedius Pseudomonas lini Duganella zoogloeoides Arthrobacter halodurans Pseudomonas chengduensis Chondromyces lanuginosus Rheinheimera hassiensis Alicycliphilus denitrificans Flavobacterium lacus Corynebacterium testudinoris Chryseobacterium anthropi Chryseobacterium soldanellicola Aeromonas lacus Alcanivorax dieselolei Lysinimonas soli Rhodovulum kholense Brevundimonas naejangsanensis Corynebacterium pseudodiphther Roseomonas oryzae Acinetobacter septicus Fusobacterium nucleatum Amaricoccus tamworthensis Veillonella parvula Prevotella jejuni Chryseobacterium piscicola Gemella taiwanensis Flavobacterium hibernum Brochothrix thermosphacta Brevundimonas poindexterae Pseudomonas koreensis Facklamia languida Comamonas jiangduensis Stenotrophomonas chelatiphaga Sanguibacter keddieii Bifidobacterium longum Pseudomonas migulae Psychrobacter aquimaris Blastococcus endophyticus Pararhizobium polonicum Geothrix fermentans Luteimonas huabeiensis
0.019 0.019 0.019 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.015 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011
0.000 0.000 0.000 0.000 0.000 0.011 0.000 0.000 0.014 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.390 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.025 1.330 0.000 0.118 0.000 0.000 0.000 0.000 0.000 1.223 0.000 0.000 0.000 0.011 0.000
0.062 0.125 0.031 0.040 0.000 0.009 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.009 0.049 0.036 0.062 0.062 0.000 0.013 0.013 0.000 0.000 0.004 0.000 0.000 0.027 0.004 0.000 0.000 0.000 0.000 0.116 0.000 0.004 0.000 0.000 0.009 0.009 0.009 0.000 0.000 0.004 0.004 0.000 0.004 0.004 0.000
0.004 0.000 0.090 0.004 0.000 0.027 0.000 0.000 0.024 0.004 0.004 0.000 0.000 0.055 0.098 0.031 0.000 0.000 0.008 0.016 0.020 0.000 0.012 0.000 0.012 0.000 0.027 0.035 0.000 0.000 0.004 0.020 0.008 0.039 0.114 0.098 0.008 0.016 0.008 0.000 0.012 0.130 0.012 0.000 0.000 0.004 0.008 0.000
0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.004
Modestobacter caceresii Achromobacter aegrifaciens Rhodococcus cerastii Alloprevotella rava Corynebacterium xerosis Psychrobacter sanguinis Comamonas testosteroni Vibrio anguillarum Halomonas boliviensis Brucella papionis Comamonas terrigena Shewanella chilikensis Luteolibacter pohnpeiensis Sphingopyxis fribergensis Rheinheimera pacifica Buttiauxella warmboldiae Erysipelothrix inopinata Pseudomonas anguilliseptica Spirosoma fluviale Sphingomonas ginsenosidivorax Finegoldia magna Ramlibacter solisilvae Vagococcus fessus Pseudorhodoferax aquiterrae Marinicella litoralis Escherichia fergusonii Epilithonimonas tenax Pseudomonas kuykendallii Pseudomonas mucidolens Rheinheimera arenilitoris Psychrobacter immobilis Corynebacterium minutissimum Methylotenera versatilis Taibaiella chishuiensis Asaia siamensis Pseudomonas fragi Flavobacterium urumqiense Paraburkholderia sabiae Limnohabitans planktonicus Psychrobacter faecalis Vagococcus carniphilus Neisseria oralis Pedobacter lotistagni Rothia dentocariosa Lactobacillus rhamnosus Pseudomonas putida Sphingobacterium faecium Chryseobacterium hispanicum
0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.008 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007
0.000 0.000 0.000 0.000 0.000 0.039 0.000 0.007 0.000 0.000 0.000 0.046 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.036 0.082 0.000 0.004 0.000 0.004 0.000 0.000 0.025 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.079 0.000
0.000 0.022 0.031 0.000 0.000 0.009 0.013 0.045 0.031 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.000 0.009 0.000 0.009 0.000 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.000 0.125 0.018 0.000 0.004 0.000 0.085 0.000 0.000 0.045 0.013 0.040 0.000 0.000 0.000 0.000 0.009 0.000 0.098 0.000
0.000 0.008 0.020 0.016 0.000 0.090 0.000 0.020 0.012 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.004 0.078 0.000 0.000 0.000 0.000 0.212 0.000 0.000 0.200 0.004 0.004 0.020 0.008 0.000 0.043 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.106 0.004 0.004 0.039 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.069 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.012 0.000
Rosenbergiella australoborealis Gemella morbillorum Psychrobacter fozii Nitrosospira multiformis Luteimonas abyssi Pseudomonas punonensis Peredibacter starrii Pseudomonas savastanoi Psychrobacter okhotskensis Methylotenera mobilis Bdellovibrio bacteriovorus Pseudomonas composti Haemophilus influenzae Weissella ceti Albirhodobacter marinus Brachybacterium faecium Exiguobacterium sibiricum Soonwooa buanensis Sulfuricella denitrificans Acinetobacter indicus Corynebacterium pyruviciproduce Streptococcus thermophilus Rhizomicrobium electricum Granulicatella elegans Pseudomonas azotoformans Acinetobacter tandoii Methylopila musalis Flavobacterium tructae Rheinheimera aquatica Deinococcus indicus Roseomonas gilardii Alkalibacterium psychrotolerans Dermacoccus profundi Myxococcus macrosporus Rubellimicrobium roseum Methylobacterium populi Niastella yeongjuensis Mesocricetibacter intestinalis Chryseobacterium solani Jiangella mangrovi Hymenobacter psychrophilus Sinorhizobium medicae Thermomonas carbonis Chryseobacterium vietnamense Shewanella dokdonensis Rubrivivax gelatinosus Chryseobacterium humi Flavobacterium noncentrifugens
0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.007 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.011 0.000 0.000 0.000 0.000 0.014 0.000 0.018 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.000 0.021 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.004 0.000 0.000 0.040 0.000 0.004 0.000 0.004 0.004 0.009 0.018 0.000 0.000 0.018 0.013 0.022 0.022 0.000 0.000 0.000 0.000 0.000 0.000 1.004 0.022 0.000 0.183 0.000 0.000 0.013 0.000 0.000 0.000 0.013 0.000 0.000 0.000 0.009 0.000 0.000 0.004 0.000 0.000 0.000 0.009 0.000 0.004
0.000 0.000 0.000 0.000 0.008 0.027 0.000 0.000 0.000 0.000 0.000 0.016 0.024 0.012 0.004 0.086 0.008 0.012 0.000 0.000 0.000 0.027 0.000 0.008 16.754 0.016 0.000 0.031 0.000 0.000 0.000 0.000 0.102 0.000 0.039 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Microbacterium pygmaeum Abiotrophia defectiva Cutibacterium granulosum Dolosigranulum pigrum Leptonema illini Thauera aminoaromatica Roseomonas cervicalis Lactococcus plantarum Sinibacillus soli Pseudopropionibacterium propio Flavobacterium columnare Methylocystis echinoides Leuconostoc carnosum Pedobacter jejuensis Devosia psychrophila Enterococcus columbae Simplicispira metamorpha Kocuria kristinae Rothia mucilaginosa Rheinheimera japonica Duganella sacchari Zymobacter palmae Nocardioides daedukensis Lactobacillus songhuajiangensis Sphingomonas panni Pantoea gaviniae Rudaea cellulosilytica Haemophilus paraphrohaemolyti Pseudomonas veronii Rheinheimera mesophila Paracoccus yeei Tahibacter aquaticus Pseudomonas marginalis Corynebacterium tuberculosteari Photobacterium phosphoreum Dietzia alimentaria Haemophilus pittmaniae Hafnia psychrotolerans Flavobacterium micromati Aliidiomarina maris Streptococcus gordonii Chitinophaga costaii Ochrobactrum rhizosphaerae Lactococcus formosensis Romboutsia sedimentorum Flavobacterium urocaniciphilum Pedobacter terrae Halomonas stenophila
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.075 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 1.437 0.000 0.000 0.000 0.000 0.000 0.018 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.009 0.004 0.027 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.049 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.009 0.000 0.013 0.004 0.580 0.000 0.000 0.000 0.000 0.000 0.000 0.112 0.000 0.000 0.000 0.000 0.116 0.000 0.000 0.004 0.004 0.004
0.000 0.039 0.004 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.004 0.000 0.000 0.000 0.024 0.043 0.000 0.000 0.012 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.012 0.000 0.024 0.008 0.000 0.008 0.000 0.039 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.032 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Bacillus alveayuensis Bdellovibrio exovorus Pedobacter arcticus Shewanella decolorationis Enterococcus gallinarum Alkanindiges illinoisensis Clostridium perfringens Corynebacterium suicordis Giesbergeria anulus Hyphomicrobium aestuarii Psychrobacter fulvigenes Phycicoccus badiiscoriae Colwellia aestuarii Acinetobacter seohaensis Simplicispira psychrophila Leucobacter chromiireducens Capnocytophaga leadbetteri Blastocatella fastidiosa Pantoea septica Massilia aerilata Clostridium baratii Proteiniphilum acetatigenes Actinomyces bowdenii Pseudomonas jessenii Planococcus halocryophilus Acinetobacter marinus Sphingomonas starnbergensis Cellulophaga tyrosinoxydans Chryseobacterium treverense Paraburkholderia oxyphila Kushneria pakistanensis Undibacterium squillarum Dietzia aurantiaca Aeromonas hydrophila Arcobacter butzleri Psychrobacter ciconiae Streptomyces klenkii Aeromonas eucrenophila Clostridium subterminale Peptoniphilus coxii Pedobacter himalayensis Chryseobacterium soli Thermicanus aegyptius Paracoccus rhizosphaerae Xylella fastidiosa Chryseobacterium marinum Methylophilus methylotrophus Alcaligenes aquatilis
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 15.310 0.000 0.000 0.000 0.039 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.004 0.018 0.009 0.000 0.000 0.000 0.000 0.013 0.004 0.022 0.004 0.062 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.022 0.000 0.000 0.004 0.018 0.000 0.000 0.000 0.004 0.013 0.000 0.000 0.000 0.004 0.000 0.000
0.000 0.008 0.000 0.000 0.000 0.024 0.004 0.012 0.000 0.000 0.000 0.035 0.008 0.004 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.016 0.020 0.000 0.055 0.000 0.000 0.000 0.008 0.000 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Myroides injenensis Marinilactibacillus psychrotoleran Actibacter sediminis Amphritea atlantica Paludibacterium yongneupense Methylocystis hirsuta Capnocytophaga sputigena Fusobacterium simiae Pseudomonas donghuensis Flavobacterium suncheonense Pseudoalteromonas shioyasakien Flavobacterium ceti Paraferrimonas sedimenticola Leptothrix mobilis Staphylococcus sciuri Flavobacterium cutihirudinis Methylobacterium phyllostachyo Tetragenococcus muriaticus Psychrobacter submarinus Lactobacillus shenzhenensis Pseudoalteromonas arabiensis Elizabethkingia endophytica Corynebacterium durum Flavobacterium lindanitolerans Psychrobacter urativorans Bradyrhizobium japonicum Acinetobacter venetianus Pelomonas saccharophila Sinorhizobium fredii Hyphomicrobium denitrificans Oxalicibacterium solurbis Undibacterium oligocarboniphilu Bacillus horikoshii Methylovirgula ligni Ahrensia kielensis Flavobacterium algicola Chiayiivirga flava Thioprofundum hispidum Lautropia mirabilis Curvibacter delicatus Paraburkholderia phytofirmans Anaerococcus prevotii Morganella morganii Flavobacterium jumunjinense Hyphomicrobium methylovorum Psychrobacter marincola Shewanella mangrovi Pseudomonas oryzihabitans
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.018 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.182 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.013 0.000 0.013 0.018 0.013 0.004 0.000 0.000 0.000 0.000 0.022 0.000 0.000 0.000 0.000 0.000 0.013 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.018 0.013 0.000 0.009 0.000 0.009 0.000 0.004 0.000 0.000 0.031 0.000 0.013 0.000 0.013
0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.012 0.039 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.157 0.000 0.035 0.000 0.000 0.004 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.004 0.000 0.016 0.000 0.012
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Pleomorphomonas diazotrophica Pseudomonas corrugata Cluster 848 Pseudoalteromonas undina Cluster 25 Staphylococcus saprophyticus Chryseobacterium oncorhynchi Cluster 1415 Micrococcus luteus Cluster 844 Leucobacter exalbidus Pseudoalteromonas luteoviolacea Stenotrophomonas humi Acinetobacter tjernbergiae Acinetobacter kookii Dermacoccus nishinomiyaensis Cluster 867 Cluster 975 Knoellia aerolata Aeromonas australiensis Janthinobacterium lividum Cluster 697 Paeniglutamicibacter antarcticus Kocuria palustris Cluster 650 Stenotrophomonas pavanii Paracoccus sanguinis Agreia pratensis Brachybacterium rhamnosum Aeromonas bivalvium Cluster 56 Pseudoclavibacter helvolus Rhizobium skierniewicense Cluster 793 Cluster 439 Cluster 37 Actinomyces naeslundii Cluster 27 Streptococcus salivarius Cluster 240 Cluster 785 Rothia aeria Gemmobacter nectariphilus Cluster 847 Bavariicoccus seileri Brevibacterium ammoniilyticum Cluster 57 Elizabethkingia miricola
0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.157 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.025 0.050 0.000 0.025 0.000 0.000 0.000 0.000 0.000 0.000 0.021 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.732 1.174 0.978 0.362 0.344 0.339 0.335 0.254 0.214 0.205 0.201 0.165 0.138 0.116 0.098 0.094 0.094 0.089 0.085 0.085 0.085 0.085 0.080 0.076 0.071 0.071 0.067 0.062 0.058 0.058 0.054 0.054 0.054 0.049 0.049 0.049 0.049 0.045 0.040 0.040 0.040 0.036 0.036 0.036 0.036 0.036 0.036
0.000 0.027 0.000 0.271 0.000 0.192 0.000 0.000 0.110 0.000 0.012 0.055 0.000 0.039 0.000 0.004 0.000 0.000 0.000 0.012 1.710 0.000 1.040 0.263 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.035 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.016
0.004 0.012 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.000 0.000 0.895 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Kluyvera intermedia Cluster 18 Alkalibacterium iburiense Cluster 611 Neisseria perflava Serratia quinivorans Anaerococcus octavius Curtobacterium plantarum Anaerococcus hydrogenalis Shewanella gaetbuli Nesterenkonia xinjiangensis Rheinheimera nanhaiensis Ancylobacter polymorphus Sphingomonas psychrolutea Pseudoalteromonas tunicata Aquihabitans daechungensis Microbacterium luteolum Delftia tsuruhatensis Vibrio toranzoniae Eubacterium yurii Paraburkholderia heleia Kroppenstedtia guangzhouensis Aeromicrobium tamlense Photobacterium piscicola Pelomonas puraquae Geminicoccus roseus Vibrio lentus Aeromonas finlandiensis Gemmatimonas aurantiaca Sanguibacter antarcticus Haemophilus parahaemolyticus Dyadobacter beijingensis Lysobacter capsici Rheinheimera muenzenbergensis Cardiobacterium hominis Brevundimonas mediterranea Rheinheimera tilapiae Methylobacterium indicum Salinicoccus qingdaonensis Neisseria elongata Mucilaginibacter sabulilitoris Skermanella stibiiresistens Pseudoxanthomonas wuyuanens Uruburuella suis Marinomonas foliarum Brachymonas denitrificans Corynebacterium amycolatum Acidovorax defluvii
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.125 0.000 0.000 0.000 0.000 0.011 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.036 0.031 0.031 0.031 0.031 0.031 0.027 0.027 0.027 0.027 0.027 0.027 0.027 0.027 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.022 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.018 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013
0.000 0.000 0.000 0.000 0.039 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.020 0.000 0.039 0.027 0.000 0.000 0.000 0.000 0.004 0.008 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.008 0.000 0.000 0.035 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Flavobacterium chungangense Bifidobacterium animalis Staphylococcus warneri Frondihabitans peucedani Acinetobacter calcoaceticus Arcobacter cryaerophilus Selenomonas noxia Diaphorobacter polyhydroxybuty Flavobacterium gyeonganense Sphingomonas pituitosa Arthrobacter agilis Myroides odoratimimus Carnobacterium funditum Undibacterium jejuense Sphingomonas canadensis Peptostreptococcus stomatis Massilia umbonata Negativicoccus succinicivorans Arcobacter venerupis Chryseobacterium contaminans Halomonas kenyensis Caulobacter segnis Subsaxibacter broadyi Prevotella oris Corynebacterium imitans Corynebacterium casei Varibaculum cambriense Lysobacter panaciterrae Pseudorhodobacter aquimaris Alkanindiges hongkongensis Gordonia terrae Labilithrix luteola Dokdonella immobilis Dermabacter hominis Nocardioides kongjuensis Pseudomonas guguanensis Desulfovibrio simplex Fluviicola hefeinensis Flavobacterium seoulense Bacteroides propionicifaciens Pseudonocardia alaniniphila Streptomyces olivoviridis Kineococcus radiotolerans Undibacterium pigrum Staphylococcus devriesei Flavobacterium fluvii Dysgonomonas gadei Enterococcus cecorum
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.013 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.008 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009
0.000 0.008 0.004 0.000 0.004 0.000 0.000 0.020 0.000 0.000 0.000 0.012 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.012 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.039 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Massilia namucuonensis Actinokineospora fastidiosa Dialister pneumosintes Altererythrobacter troitsensis Hymenobacter metalli Exiguobacterium aestuarii Comamonas composti Lysobacter brunescens Prevotella intermedia Hydrobacter penzbergensis Hydrogenophaga caeni Dongia mobilis Halodurantibacterium flavum Pseudomonas viridiflava Escherichia marmotae Prevotella loescheii Pseudomonas flexibilis Streptomyces alkalithermotolera Rubritalea spongiae Acinetobacter guangdongensis Cellulomonas massiliensis Flavobacterium frigidarium Chryseobacterium formosense Kingella oralis Chryseobacterium bovis Porphyromonas catoniae Brevibacterium aurantiacum Flavobacterium nitrogenifigens Opitutus terrae Aridibacter famidurans Corynebacterium variabile Cytophaga xylanolytica Acinetobacter gyllenbergii Roseicitreum antarcticum Pseudomonas vranovensis Cloacibacterium haliotis Mycobacterium szulgai Tetrasphaera australiensis Acinetobacter soli Hydrogenophaga taeniospiralis Lactobacillus faecis Sediminibacterium salmoneum Novosphingobium lindaniclasticu Glutamicibacter uratoxydans Citrobacter freundii Marmoricola scoriae Brevibacterium ravenspurgense Amaricoccus kaplicensis
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000
0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.016 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.012 0.008 0.000 0.000 0.000 0.020 0.000 0.137 0.000 1.043 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Lactobacillus delbrueckii Polynucleobacter asymbioticus Bacteroides acidifaciens Lactobacillus suebicus Rhizobium subbaraonis Empedobacter brevis Bizionia paragorgiae Dietzia lutea Glutamicibacter nicotianae Pseudomonas vancouverensis Paludibaculum fermentans Microbacterium ginsengiterrae Bacteroides coagulans Bacteroides cellulosilyticus Undibacterium seohonense Euzebya tangerina Rhodococcus globerulus Salinisphaera shabanensis Psychrobacter namhaensis Corynebacterium falsenii Flavobacterium hauense Citrobacter koseri Tetrasphaera duodecadis Acinetobacter brisouii Kocuria marina Lysobacter concretionis Flavobacterium aquidurense Methylocystis rosea Pseudomonas reinekei Sphingobacterium yanglingense Moraxella nonliquefaciens Streptococcus anginosus Pseudorhodobacter wandonensis Herpetosiphon aurantiacus Angiococcus disciformis Cylindrospermum licheniforme Shinella fusca Aeromonas sobria Shewanella oneidensis Corynebacterium matruchotii Pectobacterium atrosepticum Pseudoalteromonas citrea Luteimonas terricola Gibbsiella greigii Lactococcus lactis Streptococcus parasanguinis Veillonella tobetsuensis Luteimonas lutimaris
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.011 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.261 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.024 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.004 0.031 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000
0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Microbacterium maritypicum Rouxiella chamberiensis Proteiniclasticum ruminis Hydrogenophilus thermoluteolus Halomonas variabilis Methylobacterium bullatum Kurthia massiliensis Acinetobacter seifertii Nostoc punctiforme Chryseobacterium gregarium Pseudomonas cedrina Pseudomonas cuatrocienegasens Anaerococcus murdochii Psychrobacter jeotgali Zhihengliuella alba Peptoniphilus grossensis Chryseobacterium zeae Lactobacillus paracasei Parachlamydia acanthamoebae Massilia lutea Aeromonas rivuli Pseudomonas psychrophila Janthinobacterium agaricidamno Myroides profundi Aeromonas sanarellii Serratia liquefaciens Obesumbacterium proteus Carnobacterium gallinarum Ewingella americana Vagococcus penaei Morganella psychrotolerans Aeromonas fluvialis Clostridium putrefaciens Pseudomonas abietaniphila Vagococcus salmoninarum Providencia alcalifaciens Pseudomonas coleopterorum Chryseobacterium carnipullorum Pseudomonas syringae group gen Pseudomonas entomophila Aeromonas schubertii Pseudomonas protegens Serratia marcescens Hafnia alvei Brochothrix campestris Pseudomonas chlororaphis Pseudomonas trivialis Aquisalimonas lutea
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.082 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 13.230 12.611 2.130 1.004 0.447 0.361 0.232 0.147 0.093 0.064 0.064 0.036 0.036 0.021 0.021 0.018 0.018 0.014 0.014 0.011 0.011 0.007 0.007 0.008 0.007 0.007 0.004 0.004
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.008 0.004 0.000 0.000 0.004 0.000 0.004 0.000 0.000 0.016 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.012 0.000 0.000 0.000 0.078 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.106 0.000 0.000 0.000 0.000 0.000 0.004 0.667 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.008 25.215 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.004 0.000 0.000 0.000 0.000 0.000
Spirillum winogradskyi Exiguobacterium himgiriensis Azotobacter salinestris Myroides gitamensis Pseudomonas cremoricolorata Thauera phenylacetica Pseudomonas plecoglossicida Flavobacterium hydatis Flavobacterium hercynium Pseudomonas zhaodongensis Pseudomonas graminis Melissococcus plutonius Pseudomonas salomonii Pseudochrobactrum kiredjianiae Bacillus nanhaiisediminis Photobacterium kishitanii Terrimonas rubra Ochrobactrum gallinifaecis Pseudomonas brassicacearum Carnobacterium alterfunditum Ancylobacter abiegnus Vibrio litoralis Pseudomonas xinjiangensis Marinobacter nitratireducens Myroides pelagicus Halothiobacillus neapolitanus Ichthyenterobacterium magnum Myroides xuanwuensis Azomonas macrocytogenes Hafnia paralvei Oceanospirillum beijerinckii Shewanella fodinae Balneatrix alpica Niveispirillum fermenti Methylophilus leisingeri Enterobacter cloacae Gallionella capsiferriformans Flavobacterium spartansii Rheinheimera texasensis Pseudomonas panacis Pseudomonas thivervalensis Halochromatium salexigens Luteibacter rhizovicinus Zoogloea ramigera Lactobacillus graminis Salmonella enterica Enterococcus diestrammenae Massilia lurida
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.016 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.000 0.000 0.020 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.004
Myroides marinus Erwinia billingiae Aeromonas popoffii Cluster 805 Paracoccus limosus Siccibacter turicensis Cluster 633 Rivibacter subsaxonicus Hymenobacter tibetensis Agrobacterium tumefaciens Glutamicibacter mysorens Chryseobacterium hominis Novosphingobium chloroacetimid Cluster 7 Cluster 375 Cloacibacterium normanense Amnibacterium soli Cluster 799 Gemella parahaemolysans Galbitalea soli Methylobacterium trifolii Cluster 1406 Brevibacterium pityocampae Devosia limi Corynebacterium ureicelerivorans Paracoccus angustae Cluster 638 Yersinia aldovae Calothrix desertica Bacillus tequilensis Cluster 632 Chryseobacterium palustre Anaerococcus senegalensis Cluster 603 Chryseobacterium haifense Craurococcus roseus Rhizobium cellulosilyticum Comamonas denitrificans Leptotrichia buccalis Pseudomonas aeruginosa Porphyrobacter sanguineus Leucothrix pacifica Kytococcus sedentarius Pedobacter xixiisoli Corynebacterium appendicis Pseudomonas alcaligenes Streptococcus sinensis Sphingomonas ginsengisoli An et
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.228 0.169 0.145 0.145 0.110 0.110 0.106 0.090 0.086 0.078 0.075 0.071 0.067 0.055 0.055 0.051 0.051 0.051 0.051 0.047 0.043 0.039 0.039 0.035 0.035 0.036 0.035 0.035 0.035 0.031 0.031 0.024 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.016 0.016 0.016 0.016 0.016
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Cellulomonas flavigena Pantoea dispersa Nocardioides daejeonensis Rhizobium aegyptiacum Rudaeicoccus suwonensis Anaerococcus provenciensis Arthrobacter flavus Granulicatella adiacens Herbaspirillum aquaticum Adhaeribacter aquaticus Conchiformibius kuhniae Sphingobium ummariense Veillonella atypica Pseudoalteromonas translucida Methylobacterium jeotgali Sphingomonas kyungheensis Veillonella montpellierensis Erwinia iniecta Staphylococcus petrasii Acaricomes phytoseiuli Kineococcus xinjiangensis Bergeyella porcorum Parascardovia denticolens Pseudomonas duriflava Sphingomonas paucimobilis Vibrio scophthalmi Altererythrobacter marinus Georgenia halophila Bifidobacterium breve Nocardioides antarcticus Sagittula marina Facklamia tabacinasalis Phaselicystis flava Pelomonas aquatica Vibrio tasmaniensis Roseomonas terrae Devosia insulae Nitratireductor kimnyeongensis Streptococcus constellatus Aerococcus viridans Haemophilus haemolyticus Geodermatophilus africanus Sandaracinobacter sibiricus Capnocytophaga ochracea Blastochloris viridis Pseudomonas fuscovaginae Leptotrichia hongkongensis Shewanella vesiculosa
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.016 0.016 0.016 0.016 0.016 0.016 0.016 0.016 0.016 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.012 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008
0.000 0.000 0.000 0.000 0.000 0.000 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Sphingopyxis contaminans Thermus composti Clostridium clostridioforme Flavobacterium cheniae Pseudomonas straminea Flavisolibacter ginsengisoli Geobacter metallireducens Pseudomonas mediterranea Gemmobacter tilapiae Stenotrophomonas maltophilia Chryseobacterium jejuense Rhizobacter fulvus Kastovskya adunca Arthrobacter livingstonensis Pseudomonas taetrolens Corynebacterium jeikeium Pasteurella canis Thioprofundum lithotrophicum Arcobacter suis Microbacterium awajiense Rothia nasimurium Filifactor alocis Alcanivorax pacificus Staphylococcus cohnii Hyphomicrobium chloromethanic Arthrobacter russicus Acinetobacter variabilis Balneola alkaliphila Massilia varians Lewinella nigricans Deinococcus xinjiangensis Marinomonas polaris Actinomyces graevenitzii Hoeflea halophila Enterococcus alcedinis Psychrobacter aestuarii Halospirulina tapeticola Staphylococcus chromogenes Xanthomonas campestris Coprococcus catus Brevibacterium permense Enterococcus asini Modestobacter multiseptatus Sphingomonas cynarae Escherichia coli Anaerobacillus macyae Corynebacterium sputi Rubellimicrobium mesophilum
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.008 0.008 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.012 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Mycetocola lacteus Bacteroides vulgatus Comamonas nitrativorans Jannaschia faecimaris Chryseobacterium pallidum Rhodobacter blasticus Corynebacterium pilbarense Micrococcus flavus Rhizobium rosettiformans Flavobacterium aquatile Labrys wisconsinensis Dialister propionicifaciens Acidipropionibacterium jensenii Novosphingobium gossypii Pontibacter populi Flavobacterium terrae Vibrio cyclitrophicus Chryseobacterium xinjiangense Weeksella virosa Shewanella irciniae Serratia nematodiphila Pseudonocardia yuanmonensis Micrococcus lylae Halomonas songnenensis Azotobacter chroococcum Aliiroseovarius halocynthiae Nitrosococcus watsonii Capnocytophaga gingivalis Mariniflexile ostreae Peptoniphilus harei Erwinia tasmaniensis Peptoniphilus stercorisuis Janibacter cremeus Colwellia asteriadis Nitrospira moscoviensis Pseudonocardia ailaonensis Kineosporia rhizophila Microbacterium mitrae Nocardioides sediminis Taibaiella koreensis Actinomyces israelii Desemzia incerta Brevibacterium senegalense Flavobacterium rivuli Erwinia toletana Eubacterium desmolans Domibacillus indicus Tomitella biformata
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Rhizobacter gummiphilus Thauera chlorobenzoica Acidibacter ferrireducens Flavobacterium frigoris Alkalibacter saccharofermentans Dolosicoccus paucivorans Nocardioides solisilvae Celeribacter baekdonensis Rhodococcus triatomae Brevibacterium daeguense Dokdonella fugitiva Flavobacterium limicola Herbaspirillum chlorophenolicum Rhodococcus kyotonensis Ohtaekwangia koreensis Flavobacterium piscis Hydrocarboniphaga daqingensis Caloramator proteoclasticus Staphylococcus capitis Flavobacterium marinum Lysinibacter cavernae Methyloversatilis discipulorum Ferruginibacter alkalilentus Pedobacter aquatilis Brachybacterium fresconis Silanimonas mangrovi Lysobacter niastensis Marinobacter mobilis Sphingobacterium alimentarium Pandoraea pulmonicola Pseudomonas palleroniana Pectobacterium cacticida Methylobacillus glycogenes Marinobacter lutaoensis Methylocystis bryophila Methylobacillus flagellatus Modicisalibacter tunisiensis Massilia kyonggiensis Thalassomonas haliotis Azovibrio restrictus Selenomonas artemidis Intestinimonas butyriciproducens Zoogloea caeni Tolumonas auensis Flavobacterium weaverense Shannon Value CHAO1 Richness Estimation
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 3.445 675
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 2.884 532
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 3.868 722
0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 3.190 684
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.903 401
ets (including those present at <1% of the global microbiome). y. .
Table 6‐ Sequencing statistics for the fungal ITS microbiome libraries prepared from control and The number of Number of raw, paired‐end and filtered reads, average length (in base pairs, bp and average quality (Phred Q value) are shown for each sea bass muscle microbiome library. Library C11 HP67 Total Average
Raw reads Paired‐end reads Filtered reads Av. Length Total Mb Av. Qual. 78186 36491 34520 387 13.4 36.7 116874 29029 28904 278 8.0 38.5 195060 65520 63424 665 21.4 75.2 97530 32760 31712 332 10.7 37.6
d HPP sea bass fillets at the end of storage. )
Table 7‐ Main fungal genera detected by ITS sequencing, at >1% of all the genera detected per libra The rela ve propor on of a given genera rela ve to the total reads iden fied in each library is shown as a pe Only genera detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the microbiome they are shaded from grey (low proportion) to red (high proportion).
Main genus Saccharomyces Malassezia Cordyceps Candida Aspergillus Rhodotorula Kurtzmaniella Cladosporium Penicillium Urtica Verrucaria Cluster 46 Metschnikowia
C11 96.842 0.539 0.316 0.226 0.153 0.139 0.090 0.076 0.073 0.014 0.000 0.000 0.000
HP67 0.332 4.013 8.923 51.253 3.217 1.923 2.058 7.154 2.458 1.169 1.623 1.623 1.494
ary. rcentage.
Table 8- All fungal genera detected by ITS sequencing, including genera represented at low propor o Values represent the relative proportion of each genus relative to the total reads identified in each library. All detected genera are listed but those at < 1% of the total metagenome in each library are shaded in grey.
Main genus C11 HP67 Saccharomyces 96.842 0.332 Malassezia 0.539 4.013 Cordyceps 0.316 8.923 Candida 0.226 51.253 Cluster 130 0.223 0.000 Mortierella 0.156 0.000 Aspergillus 0.153 3.217 Rhodotorula 0.139 1.923 Peniophora 0.116 0.000 Didymella 0.098 0.000 Kurtzmaniella 0.090 2.058 Itersonilia 0.084 0.000 Phanerochaete 0.078 0.249 Cladosporium 0.076 7.154 Pichia 0.076 0.585 Penicillium 0.073 2.458 No Hit 0.055 0.325 Schizophyllum 0.055 0.000 Theobroma 0.046 0.000 Antrodia 0.038 0.367 Debaryomyces 0.038 0.000 Cutaneotrichospor 0.032 0.518 Parastagonospora 0.032 0.235 Phlebia 0.032 0.000 Ramularia 0.032 0.000 Sistotremastrum 0.026 0.931 Alternaria 0.026 0.010 Cluster 172 0.023 0.000 Meyerozyma 0.023 0.000 Platanus 0.020 0.339 Carex 0.020 0.000 Diplosphaera 0.017 0.000 Urtica 0.014 1.169 Aegopodium 0.014 0.000 Fragaria 0.014 0.000 Salvia 0.012 0.318 Fusarium 0.012 0.104 Galactomyces 0.012 0.000 Candida 0.012 0.000 Lecania 0.009 0.000 Sporidiobolus 0.009 0.000 Stellaria 0.009 0.000 Juglans 0.009 0.000
Yarrowia Wallemia Linum Spinacia Diplotaxis Petroselinum Cannabis Bacillus Pistacia Triticum [0]Eukaryota;[1]nu Trichoderma Saitozyma [0]Eukaryota;[1]As Zygosaccharomyce Lactobacillus Cryptococcus Achillea Verrucaria Cluster 46 Metschnikowia Cluster 77 Lophodermium Cluster 52 [0]Eukaryota;[1]As Rhizopus Cluster 154 Myrmecridium Cystobasidium Vishniacozyma Spegazzinia Entoloma Coprinopsis Cluster 121 Blumeria Gloeocystidiellum Cluster 70 Burgoa Brassica Lepista Vibrissea Verrucocladospori Vaginatispora Cluster 78 Plantago [0]Eukaryota;[1]Ba Mercurialis Deconica
0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.006 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.297 0.280 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.359 0.003 0.003 0.000 0.000 0.000 0.000 0.000 0.000 1.623 1.623 1.494 0.848 0.443 0.381 0.336 0.329 0.329 0.329 0.297 0.284 0.239 0.235 0.235 0.225 0.221 0.218 0.211 0.208 0.183 0.176 0.173 0.173 0.170 0.163 0.163 0.163 0.159 0.128
Aleurodiscus 0.000 Trebouxia 0.000 Fuscoporia 0.000 Cluster 43 0.000 Elymus 0.000 Cluster 48 0.000 Prunus 0.000 Cluster 5 0.000 Cynodon 0.000 Lecanicillium 0.000 Cystofilobasidium 0.000 Cluster 28 0.000 Cluster 30 0.000 Fraxinus 0.000 Polyporus 0.000 Kazachstania 0.000 Sclerotinia 0.000 Rhizoctonia 0.000 Lactococcus 0.000 Grosmannia 0.000 Neottiosporina 0.000 Lophiostoma 0.000 Shannon Value 0.24319
0.114 0.107 0.104 0.090 0.055 0.052 0.048 0.038 0.034 0.034 0.028 0.024 0.024 0.021 0.014 0.003 0.003 0.003 0.003 0.003 0.003 0.003 2.2408
ons (<1% of all the genera detected) per library.
Table 9‐ Main fungal species detected by ITS sequencing, at >1% of all the species detected p The rela ve propor on of a given species rela ve to the total reads iden fied in each library is shown Only species detected at >1% of the global fungal metagenome in at least one library are presented. To facilitate perception of the relative proportion of genera in the metagenome they are shaded from (low proportion) to red (high proportion).
Main species Saccharomyces cerevisiae Malassezia restricta Cordyceps confragosa Candida hollandica Aspergillus vitricola Candida zeylanoides Cladosporium sp. Candida sp. WSYC 780 Penicillium sp. NX‐deepsea Rhodotorula mucilaginosa Cluster 46 Verrucaria csernaensis Metschnikowia bicuspidata Urtica membranacea
C11 96.749 0.443 0.316 0.165 0.136 0.09 0.076 0 0 0 0 0 0 0
HP67 0.332 3.186 8.923 2.038 2.778 2.058 7.151 48.841 1.847 1.667 1.623 1.623 1.491 1.169
per library. as a percentage. m grey
Figure 1 – Alpha rarefaction 16S
Figure 2 – Alpha rarefaction ITS
Figure 3 – Venn diagram of proteins with changed levels Myofibrillar HP1vsC1
HP67vsC11
11
Sarcoplasmic HP67vsC11
HP1vsC1
15 4
37
176
49
CONFLICT of INTEREST STATEMENT
I declare on behalf of all the authors that they have no conflict of interest. •
None of the authors have financial/personal interests or belief that may affect their objectivity;
•
None of the authors have competing interests as a result of personal or academic relationships;
•
The MS has not been submitted to another journal and will not be submitted in the future should it be accepted by DiB. Yours sincerely Deborah M Power Corresponding author 07 June 2019
Universidade do Algarve - Campus de Gambelas - 8000-810 - FARO - Portugal + 351 289 800 900 + 351 289 818 353 [email protected]://www.ualg.pt/ccmar