- Email: [email protected]
Expression and functional relevance of NADPH oxidase 5 in human hepatic stellate cells
S86
A. Stepanova et al. / Free Radical Biology and Medicine 120 (2018) S45–S166
P-136
Collectively, these results suggest for the first time that Nox5 can play a relevant role in HSC proliferation and fibrogenesis.
E-mail address: [email protected]
Complex mix of Psidium Guajava. L as a protector of medicines used in the treatment of gastric ulcer
http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.284
Julie Gesselle Benavides Melo, Paola Andrea Muñoz Castro, Laura Nataly Valderrama Perez Universidad Pedagógica Nacional de Colombia, Bogotá, Colombia
Metabolic reactions that favor the production of free radicals generate oxidative stress and chain reactions, which result in illnesses such as gastritis, cancer, arthritis, etc.* Gastric ulcer is deemed as an inflammatory pathology related to an infection by H. pylori, whose treatment includes tetracycline and omeprazole. Furthermore, the hypochlorous acid (HOCl) is released in the lymphocytes as immune response to inflammatory processes through in situ oxidation reactions. HOCl has been proven to degrade these medicines* and the treatment does not consider external factors that cause health problems (harmful lifestyles, stress, and environmental factors). In this context, we propose an alternative treatment to gastric ulcer based on spectroscopic and chromatographic results including the species Psidium Guajava L as gastric protector for combined treatment. This species has antioxidant properties, associated to ascorbic acid, carotenoids, and phenolic compounds*; anti-inflammatory properties thanks to flavonoids; analgesic properties due to triterpenes; and antibacterial properties because of tannins. **
E-mail address: [email protected]; dqu_pamunozc328@ pedagogica.edu.co; [email protected] http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.283
P-137
P-138
Redox status in breast tumor and associated adipose tissue - interplay between obesity and malignancy Andjelika Kalezic, Mirjana Udicki, Sava Masovic, Biljana Srdic, Aleksandra Korac, Aleksandra Jankovic, Bato Korac Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia
Evidence linking obesity to breast cancer in premenopausal women remains inconclusive. We aimed to reveal redox status related to malignant phenotype and its relationship with obesity. To this end, premenopausal women with breast tumors were divided into four groups: normal-weight (non-obese) with benign tumors, obese with benign tumors, non-obese with malignant tumors and obese with malignant tumors. We examined activity and protein expression of main antioxidant defense (AD) enzymes (copper, zinc- and manganese- superoxide dismutase, catalase and glutathione peroxidase) in breast tumor tissue and tumor-associated adipose tissue. Activity and protein expression of AD enzymes were generally increased in malignant tumors of non-obese and obese women, in comparison to their respective benign counterparts. In tumor-associated adipose tissue of non-obese women, activity and protein expression of AD enzymes were increased in subjects with malignant tumors in comparison to those with benign tumors. In conclusion, the effect of malignancy on the redox signature of tumor tissue is evident in both non-obese and obese subjects, while in adipose tissue the effect is pronounced mostly in non-obese subjects, suggesting redox sensitive interplay between obesity and cancer.
Expression and functional relevance of NADPH oxidase 5 in human hepatic stellate cells
E-mail address: [email protected] http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.285
Aitor Andueza, Naiara Garde, Antonia García-Garzón, Eduardo Ansorena, María J. Iraburu, María J. López-Zabalza, Guillermo Zalba, Juan J. Martínez-Irujo Department of Biochemistry and Genetics, University of Navarra, Pamplona, Spain
P-139
Developing targeted mass spectrometry approaches for the identification of protein lipoxidation NADPH oxidase (Nox) variants Nox1, Nox2 and Nox4 have been implicated in the progression of liver fibrosis. However, the role of Nox5 is unknown, mainly due to the lack of this enzyme in rat and mouse genomes. Here we describe the expression and functional relevance of Nox5 in the human cell line of hepatic stellate cells (HSC), LX-2. Under basal conditions, these cells expressed a long (Nox5L) and a short (Nox5S) variant which were silenced with specific siRNAs for Nox5. Overexpression of Nox5L generated ROS in the presence of calcium, as judged by the production of extracellular hydrogen peroxide, L-012 luminescence and cytochrome c reduction, while Nox5S did not generated ROS under these conditions. In contrast, dihydroethidium oxidation was increased when either Nox5L or Nox5S were overexpressed. Functional studies revealed that both Nox5L and Nox5S stimulated the proliferation of LX-2 cells and the synthesis of type I collagen, while Nox5 siRNAs inhibited these effects. Interestingly, TGF-beta and angiotensin II induced Nox5, and silencing Nox5 reduced collagen production stimulated by TGF-beta.
Corinne M. Spickett 1, Eleanor J.B. Bhurrut 1, Bebiana C. Sousa 1, Catarina B. Afonso 1, Thierry Durand 2, Andrew R. Pitt 1 1
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK 2 Institut des Biomolécules Max Mousseron (IBMM) , Faculté de Pharmacie, UMR 5247 CNRS - UM – ENSCM, France
Lipid peroxidation leads to formation of a variety of reactive products, including short-chain aldehydes. These can covalently modify proteins, a process called “lipoxidation”, and are thought to be involved in inflammatory diseases, as well as having potential as biomarkers. Our aim was to develop label-free mass spectrometry (MS) methods for the identification of aldehyde
A. Stepanova et al. / Free Radical Biology and Medicine 120 (2018) S45–S166
P-136
Collectively, these results suggest for the first time that Nox5 can play a relevant role in HSC proliferation and fibrogenesis.
E-mail address: [email protected]
Complex mix of Psidium Guajava. L as a protector of medicines used in the treatment of gastric ulcer
http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.284
Julie Gesselle Benavides Melo, Paola Andrea Muñoz Castro, Laura Nataly Valderrama Perez Universidad Pedagógica Nacional de Colombia, Bogotá, Colombia
Metabolic reactions that favor the production of free radicals generate oxidative stress and chain reactions, which result in illnesses such as gastritis, cancer, arthritis, etc.* Gastric ulcer is deemed as an inflammatory pathology related to an infection by H. pylori, whose treatment includes tetracycline and omeprazole. Furthermore, the hypochlorous acid (HOCl) is released in the lymphocytes as immune response to inflammatory processes through in situ oxidation reactions. HOCl has been proven to degrade these medicines* and the treatment does not consider external factors that cause health problems (harmful lifestyles, stress, and environmental factors). In this context, we propose an alternative treatment to gastric ulcer based on spectroscopic and chromatographic results including the species Psidium Guajava L as gastric protector for combined treatment. This species has antioxidant properties, associated to ascorbic acid, carotenoids, and phenolic compounds*; anti-inflammatory properties thanks to flavonoids; analgesic properties due to triterpenes; and antibacterial properties because of tannins. **
E-mail address: [email protected]; dqu_pamunozc328@ pedagogica.edu.co; [email protected] http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.283
P-137
P-138
Redox status in breast tumor and associated adipose tissue - interplay between obesity and malignancy Andjelika Kalezic, Mirjana Udicki, Sava Masovic, Biljana Srdic, Aleksandra Korac, Aleksandra Jankovic, Bato Korac Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia
Evidence linking obesity to breast cancer in premenopausal women remains inconclusive. We aimed to reveal redox status related to malignant phenotype and its relationship with obesity. To this end, premenopausal women with breast tumors were divided into four groups: normal-weight (non-obese) with benign tumors, obese with benign tumors, non-obese with malignant tumors and obese with malignant tumors. We examined activity and protein expression of main antioxidant defense (AD) enzymes (copper, zinc- and manganese- superoxide dismutase, catalase and glutathione peroxidase) in breast tumor tissue and tumor-associated adipose tissue. Activity and protein expression of AD enzymes were generally increased in malignant tumors of non-obese and obese women, in comparison to their respective benign counterparts. In tumor-associated adipose tissue of non-obese women, activity and protein expression of AD enzymes were increased in subjects with malignant tumors in comparison to those with benign tumors. In conclusion, the effect of malignancy on the redox signature of tumor tissue is evident in both non-obese and obese subjects, while in adipose tissue the effect is pronounced mostly in non-obese subjects, suggesting redox sensitive interplay between obesity and cancer.
Expression and functional relevance of NADPH oxidase 5 in human hepatic stellate cells
E-mail address: [email protected] http://dx.doi.org/10.1016/j.freeradbiomed.2018.04.285
Aitor Andueza, Naiara Garde, Antonia García-Garzón, Eduardo Ansorena, María J. Iraburu, María J. López-Zabalza, Guillermo Zalba, Juan J. Martínez-Irujo Department of Biochemistry and Genetics, University of Navarra, Pamplona, Spain
P-139
Developing targeted mass spectrometry approaches for the identification of protein lipoxidation NADPH oxidase (Nox) variants Nox1, Nox2 and Nox4 have been implicated in the progression of liver fibrosis. However, the role of Nox5 is unknown, mainly due to the lack of this enzyme in rat and mouse genomes. Here we describe the expression and functional relevance of Nox5 in the human cell line of hepatic stellate cells (HSC), LX-2. Under basal conditions, these cells expressed a long (Nox5L) and a short (Nox5S) variant which were silenced with specific siRNAs for Nox5. Overexpression of Nox5L generated ROS in the presence of calcium, as judged by the production of extracellular hydrogen peroxide, L-012 luminescence and cytochrome c reduction, while Nox5S did not generated ROS under these conditions. In contrast, dihydroethidium oxidation was increased when either Nox5L or Nox5S were overexpressed. Functional studies revealed that both Nox5L and Nox5S stimulated the proliferation of LX-2 cells and the synthesis of type I collagen, while Nox5 siRNAs inhibited these effects. Interestingly, TGF-beta and angiotensin II induced Nox5, and silencing Nox5 reduced collagen production stimulated by TGF-beta.
Corinne M. Spickett 1, Eleanor J.B. Bhurrut 1, Bebiana C. Sousa 1, Catarina B. Afonso 1, Thierry Durand 2, Andrew R. Pitt 1 1
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK 2 Institut des Biomolécules Max Mousseron (IBMM) , Faculté de Pharmacie, UMR 5247 CNRS - UM – ENSCM, France
Lipid peroxidation leads to formation of a variety of reactive products, including short-chain aldehydes. These can covalently modify proteins, a process called “lipoxidation”, and are thought to be involved in inflammatory diseases, as well as having potential as biomarkers. Our aim was to develop label-free mass spectrometry (MS) methods for the identification of aldehyde