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Expression and induction of heme oxygenase mRNA in rat placenta
Abstracts: J.P.G. Japan 1995
EXPRESSION AND INDUCTION OF HEME OXYGENASE mRNA IN RAT PLACENTA. Naomi Ihara, Yuji Hiramatsu, Hiroyuki Okuda, Takahumi Kudo and Reiko Akagi, Department of Obstetrics and Gynecology, Okayama University Medical School, Faculty of Health and Welfare Science, Okayama Prefectural University, Okayama, Japan Heme oxygenase (HO), a rate-limiting enzyme in heme catabolism, cleaves heme to form biliverdin, iron and carbon monoxide (CO). It is thought to function as a defense system against several kinds of stress because of the end product bilirubin which is one of endogenous radical scavengers. Recently it was suggested that CO produced by the HO reaction functions as an endogenous modulator of vascular one. The fact that the fetus lives under hypoxic conditions prompted us to investigate the statistics of HO expression in the placenta and fetal tissues. HO mRNA was detected by Northern blot analysis using biotin labeled specific RNA probe. HO mRNA showed significantly higher levels in placenta compared to other rat fetal tissues and was almost the same level of adult spleen. It increased during pregnancy, reached the highest on the 17th day of gestation. Ligature of uterine vessels resulted in the induction of HO within 2 hours and reached more than two times of the basal level. Our findings suggest that heme might be primarily catabolized in the placenta instead of the spleen during the fetal period and CO produced by the HO reaction may contibute as a modulator of the fetoplacental circulation.
A CASE OF PLACENTAL SITE TROPHOBLASTIC TUMOR (PSTT) WITH NO RECURRENCE FOR 8 YEARS AFTER TREATMENT. Kenichiro Ikuma”, Toshiya Komatsu” and Tadashi Takemura”, Department of Obstetrics and Gynecology” and Division of Pathology”, Takarazuka City Hospital, Hyogo, Department of Obstetrics and Gynecology, Hyogo Medical College’, Hyogo , Japan (Objective) The histological diagnosis of a biopsy specimen was glassy cell carcinoma, Placental site trophoblastic tumor (PSTT) was suspected and later confirmed. We have followed the patient for 8 years and noted no abnormal findings. (Case Report) A 44-year-old woman had had two normal deliveries and two artificial terminations of pregnancy, most recently in 1980. On October 27, 1988, she visited our hospital because of bleeding during coitus. A dark red polyp-like protrusion was seen in the uterine cervix. Cytological and histological examinations led to a definite diagnosis of PSTT. Tumor markers before surgery were within the normal range; hCG, less than 2.0 mIU/ml; ,0 -hCG, less than 0.6 rig/ml; hPL, less than 1 g/ml. (Clinical course) She was admitted to our hospital on December 1, 1988, and abdominal hysterectomy and right adnectomy were performed on December 5. Her postoperative recovery was good, and she was discharged on December 20,1988 after intraperitoneal administration of 80 mg of CDDP. Radiation therapy was started in January 1989. Etoposide was administered orally every other week at a daily dose of 25 mg for 6 months. (Conclusion) Previous reports of PSTT have indicated that the prognosis is relatively poor in patients with many karyokinetic figures and high values of hCG. Further elucidation of the pathophysiology of this disease and the establishment of appropriate therapy are needed, but we believe that surgery is still the best initial therapy.
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EXPRESSION AND INDUCTION OF HEME OXYGENASE mRNA IN RAT PLACENTA. Naomi Ihara, Yuji Hiramatsu, Hiroyuki Okuda, Takahumi Kudo and Reiko Akagi, Department of Obstetrics and Gynecology, Okayama University Medical School, Faculty of Health and Welfare Science, Okayama Prefectural University, Okayama, Japan Heme oxygenase (HO), a rate-limiting enzyme in heme catabolism, cleaves heme to form biliverdin, iron and carbon monoxide (CO). It is thought to function as a defense system against several kinds of stress because of the end product bilirubin which is one of endogenous radical scavengers. Recently it was suggested that CO produced by the HO reaction functions as an endogenous modulator of vascular one. The fact that the fetus lives under hypoxic conditions prompted us to investigate the statistics of HO expression in the placenta and fetal tissues. HO mRNA was detected by Northern blot analysis using biotin labeled specific RNA probe. HO mRNA showed significantly higher levels in placenta compared to other rat fetal tissues and was almost the same level of adult spleen. It increased during pregnancy, reached the highest on the 17th day of gestation. Ligature of uterine vessels resulted in the induction of HO within 2 hours and reached more than two times of the basal level. Our findings suggest that heme might be primarily catabolized in the placenta instead of the spleen during the fetal period and CO produced by the HO reaction may contibute as a modulator of the fetoplacental circulation.
A CASE OF PLACENTAL SITE TROPHOBLASTIC TUMOR (PSTT) WITH NO RECURRENCE FOR 8 YEARS AFTER TREATMENT. Kenichiro Ikuma”, Toshiya Komatsu” and Tadashi Takemura”, Department of Obstetrics and Gynecology” and Division of Pathology”, Takarazuka City Hospital, Hyogo, Department of Obstetrics and Gynecology, Hyogo Medical College’, Hyogo , Japan (Objective) The histological diagnosis of a biopsy specimen was glassy cell carcinoma, Placental site trophoblastic tumor (PSTT) was suspected and later confirmed. We have followed the patient for 8 years and noted no abnormal findings. (Case Report) A 44-year-old woman had had two normal deliveries and two artificial terminations of pregnancy, most recently in 1980. On October 27, 1988, she visited our hospital because of bleeding during coitus. A dark red polyp-like protrusion was seen in the uterine cervix. Cytological and histological examinations led to a definite diagnosis of PSTT. Tumor markers before surgery were within the normal range; hCG, less than 2.0 mIU/ml; ,0 -hCG, less than 0.6 rig/ml; hPL, less than 1 g/ml. (Clinical course) She was admitted to our hospital on December 1, 1988, and abdominal hysterectomy and right adnectomy were performed on December 5. Her postoperative recovery was good, and she was discharged on December 20,1988 after intraperitoneal administration of 80 mg of CDDP. Radiation therapy was started in January 1989. Etoposide was administered orally every other week at a daily dose of 25 mg for 6 months. (Conclusion) Previous reports of PSTT have indicated that the prognosis is relatively poor in patients with many karyokinetic figures and high values of hCG. Further elucidation of the pathophysiology of this disease and the establishment of appropriate therapy are needed, but we believe that surgery is still the best initial therapy.
A.3