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Expression of a gene involved in biohydrogen production in Cyanobacterium syenchococcus sp. PCC 7002 under environmental stress conditions
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Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
In this study, the possibility of using rapeseed oil as a carbon source for microbiological production of KGA has been studied. KGA production has been tested in 30 strains of Yarrowia lipolytica yeast, and the strain Y. lipolytica 212 was selected as a prospective producer of KGA. KGA production by the producer was studied in dependence on thiamine concentration, medium pH, temperature, aeration and concentration of oil in the medium. Under optimal conditions Y. lipolytica 212 produced 102.5 g/L of KGA with the yield of 95%. Acknowledgements: This work was supported by the Ministry of Education and Science of the Russian Federation (grant no. 14.512.11.0045). http://dx.doi.org/10.1016/j.copbio.2013.05.220 Expression of a gene involved in biohydrogen production in Cyanobacterium syenchococcus sp. PCC 7002 under environmental stress conditions Bogdan Druga 1 , Cristian Coman 1 , Adriana Hegedus 1 , Cosmin Sicora 2 1
Institute of Biological Research, Cluj-Napoca, Romania Center of Biological Research, Jibou, Romania E-mail address: [email protected] (B. Druga). 2
Hydrogen is a clean-burning alternative to fossil fuels. Its energy content per unit mass is higher than any other conventional fuel, except for nuclear power. It is eco-friendly, efficient and renewable. By the virtue of all these attributes the hydrogen gas can be used as an energy source. The most cost-effective way to produce hydrogen is to use microorganisms able to perform photosynthesis, like cyanobacteria. H2 is produced via a group of metalloenzymes called hydrogenases, which catalyse the reaction 2H+ + 2e− ↔ H2 . In this study we analyzed the cyanobacterial strain Synechococcus sp. PCC 7002, in order to clarify the expression of the hoxH (hydrogenase) under certain environmental factors. Three to five cultures under exponential growth phase were investigated under three stress conditions: high light, UV-B irradiance and microaerobiosis, followed by a recovery period with the standard conditions. Each culture was sampled three times during stress conditions, and two more times during the recovery period. The samples were used for total RNA extraction, and it was used for analyzing the expression pattern of the hoxH gene by real time PCR using specific oligonucleotidic primers. The hoxH gene has not displayed a very high level of expression under microaerobiosis, confirming that this occurs mainly in filamentous species. Moreover, high light and the UV-B irradiance have influenced the expression of the hoxH gene, but not at a very high level. More interesting results have been achieved with the samples from the recovery period, the target-gene displaying divergent expression patterns.
research was to establish an effective method of seeds separation from tomato pomace for further extraction of oil. For this research seven different samples of tomato pomace (containing peels, seeds and a very small amount of residual pulp) were produced from a cold break process, then were mixed with yeast solution of different concentrations (0.10%, 0.15%, respectively 0.20%) and left to ferment 2–3 days. The upper phase contains peels and lower phase is represented by the seeds. The seeds were separated, washed, dried and used for oil extraction. The amount of seeds was determined on a dry weight basis. The method used has revealed good separation results. Seed recovery was higher comparing with other methods that does not require yeast for separation. This research indicated that separation of tomato seeds by using wet fermentation technology is a promising option. The use of an efficient method for seeds separation is an important parameter in order to optimize the production of tomato seed oil for industrial purposes. http://dx.doi.org/10.1016/j.copbio.2013.05.222 Research on the temperatures and precipitation in Timis¸ County and their effect on forest plantations in the scope of relief from Becicherecu Mic Banu Constantin, Banu Tiberiu, Banu Cristina Banat’s University of Agricultural Science and Veterinary Medicine from Timisoara, Romania E-mail address: [email protected] (B. Constantin). The air temperature is a key element that can influence the development of drought phenomena which in turn arises leads to calamity many agricultural crops and forestry. To determine the causes of forest crop dry mass installed in the perimeter of the Becicherecu Mic to analyze developments in the last three years the air temperature as a determining factor for the occurrence of droughts. Disasters on forest crop area Becicherecu Mic improvement was due to the drought which in turn arose as a result of increases in average temperatures about 2–5◦ C, during June–August 2012 compared to the same months in 2010–2011. The time at which precipitation falls and their quantity are important elements of analysis to study the influence of rainfall on the occurrence of droughts which in turn leads to calamity crops and forestry. To determine the causes of forest crop dry mass installed in the perimeter of the Becicherecu Mic to analyze the evolution of rainfall over the past three years as a determining factor for the occurrence of droughts. Deficit rainfall in this period compared to evapotranspiration is the amount of dry nature of the climate and the soil, requiring irrigation, forestry and agricultural crops. For a sum of Banat Plain rainfall in May, June and July of less than 100 mm compromise the crop. http://dx.doi.org/10.1016/j.copbio.2013.05.223
http://dx.doi.org/10.1016/j.copbio.2013.05.221 Tomato seed oil for industrial use: separation of seeds from tomato pomace using wet fermentation technology Cristina Botinestean, Ionel Jianu Banat’s University of Agricultural Sciences and Veterinary Medicine, Faculty of Food Processing Technology, Timis¸oara, Romania E-mail address: [email protected] (C. Botinestean). Tomatoes are among the most cultivated vegetable in the world due to their valuable nutritive composition. The large amount of wastes that results from tomato processing industry is an important environmental and economically issue. The objective of this
Efficient electricity generation from donkey farm wastewater by using microbial fuel cells Jingfei Luan 1 , Mengjing Chen 1 , Zhigang Zou 2 1 State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, China 2 Eco-Materials and Renewable Energy Research Center, Nanjing University, China E-mail address: [email protected] (M. Chen).
We treated donkey farm wastewater (DFW) and produced efficient electricity power (EP) by using microbial fuel cell (MFC). First we utilized a two-chambered MFC with an aqueous cathode and the
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
In this study, the possibility of using rapeseed oil as a carbon source for microbiological production of KGA has been studied. KGA production has been tested in 30 strains of Yarrowia lipolytica yeast, and the strain Y. lipolytica 212 was selected as a prospective producer of KGA. KGA production by the producer was studied in dependence on thiamine concentration, medium pH, temperature, aeration and concentration of oil in the medium. Under optimal conditions Y. lipolytica 212 produced 102.5 g/L of KGA with the yield of 95%. Acknowledgements: This work was supported by the Ministry of Education and Science of the Russian Federation (grant no. 14.512.11.0045). http://dx.doi.org/10.1016/j.copbio.2013.05.220 Expression of a gene involved in biohydrogen production in Cyanobacterium syenchococcus sp. PCC 7002 under environmental stress conditions Bogdan Druga 1 , Cristian Coman 1 , Adriana Hegedus 1 , Cosmin Sicora 2 1
Institute of Biological Research, Cluj-Napoca, Romania Center of Biological Research, Jibou, Romania E-mail address: [email protected] (B. Druga). 2
Hydrogen is a clean-burning alternative to fossil fuels. Its energy content per unit mass is higher than any other conventional fuel, except for nuclear power. It is eco-friendly, efficient and renewable. By the virtue of all these attributes the hydrogen gas can be used as an energy source. The most cost-effective way to produce hydrogen is to use microorganisms able to perform photosynthesis, like cyanobacteria. H2 is produced via a group of metalloenzymes called hydrogenases, which catalyse the reaction 2H+ + 2e− ↔ H2 . In this study we analyzed the cyanobacterial strain Synechococcus sp. PCC 7002, in order to clarify the expression of the hoxH (hydrogenase) under certain environmental factors. Three to five cultures under exponential growth phase were investigated under three stress conditions: high light, UV-B irradiance and microaerobiosis, followed by a recovery period with the standard conditions. Each culture was sampled three times during stress conditions, and two more times during the recovery period. The samples were used for total RNA extraction, and it was used for analyzing the expression pattern of the hoxH gene by real time PCR using specific oligonucleotidic primers. The hoxH gene has not displayed a very high level of expression under microaerobiosis, confirming that this occurs mainly in filamentous species. Moreover, high light and the UV-B irradiance have influenced the expression of the hoxH gene, but not at a very high level. More interesting results have been achieved with the samples from the recovery period, the target-gene displaying divergent expression patterns.
research was to establish an effective method of seeds separation from tomato pomace for further extraction of oil. For this research seven different samples of tomato pomace (containing peels, seeds and a very small amount of residual pulp) were produced from a cold break process, then were mixed with yeast solution of different concentrations (0.10%, 0.15%, respectively 0.20%) and left to ferment 2–3 days. The upper phase contains peels and lower phase is represented by the seeds. The seeds were separated, washed, dried and used for oil extraction. The amount of seeds was determined on a dry weight basis. The method used has revealed good separation results. Seed recovery was higher comparing with other methods that does not require yeast for separation. This research indicated that separation of tomato seeds by using wet fermentation technology is a promising option. The use of an efficient method for seeds separation is an important parameter in order to optimize the production of tomato seed oil for industrial purposes. http://dx.doi.org/10.1016/j.copbio.2013.05.222 Research on the temperatures and precipitation in Timis¸ County and their effect on forest plantations in the scope of relief from Becicherecu Mic Banu Constantin, Banu Tiberiu, Banu Cristina Banat’s University of Agricultural Science and Veterinary Medicine from Timisoara, Romania E-mail address: [email protected] (B. Constantin). The air temperature is a key element that can influence the development of drought phenomena which in turn arises leads to calamity many agricultural crops and forestry. To determine the causes of forest crop dry mass installed in the perimeter of the Becicherecu Mic to analyze developments in the last three years the air temperature as a determining factor for the occurrence of droughts. Disasters on forest crop area Becicherecu Mic improvement was due to the drought which in turn arose as a result of increases in average temperatures about 2–5◦ C, during June–August 2012 compared to the same months in 2010–2011. The time at which precipitation falls and their quantity are important elements of analysis to study the influence of rainfall on the occurrence of droughts which in turn leads to calamity crops and forestry. To determine the causes of forest crop dry mass installed in the perimeter of the Becicherecu Mic to analyze the evolution of rainfall over the past three years as a determining factor for the occurrence of droughts. Deficit rainfall in this period compared to evapotranspiration is the amount of dry nature of the climate and the soil, requiring irrigation, forestry and agricultural crops. For a sum of Banat Plain rainfall in May, June and July of less than 100 mm compromise the crop. http://dx.doi.org/10.1016/j.copbio.2013.05.223
http://dx.doi.org/10.1016/j.copbio.2013.05.221 Tomato seed oil for industrial use: separation of seeds from tomato pomace using wet fermentation technology Cristina Botinestean, Ionel Jianu Banat’s University of Agricultural Sciences and Veterinary Medicine, Faculty of Food Processing Technology, Timis¸oara, Romania E-mail address: [email protected] (C. Botinestean). Tomatoes are among the most cultivated vegetable in the world due to their valuable nutritive composition. The large amount of wastes that results from tomato processing industry is an important environmental and economically issue. The objective of this
Efficient electricity generation from donkey farm wastewater by using microbial fuel cells Jingfei Luan 1 , Mengjing Chen 1 , Zhigang Zou 2 1 State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, China 2 Eco-Materials and Renewable Energy Research Center, Nanjing University, China E-mail address: [email protected] (M. Chen).
We treated donkey farm wastewater (DFW) and produced efficient electricity power (EP) by using microbial fuel cell (MFC). First we utilized a two-chambered MFC with an aqueous cathode and the