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Feasibility of transdermal delivery of lidocaine-loaded nanocarriers through artificial and human skin: a pilot study
Poster Abstracts
Feasibility of transdermal delivery of lidocaine-loaded nanocarriers through artificial and human skin: a pilot study Krisztina Emeriewen, Katerina Lalatsa, George Saleh
Abstract Background There is a growing demand for painless, rapid onset, long duration anaesthesia in many aspects of medicine. Nanomedicines (particles 50–500 nm) have shown promise in topical delivery of certain drugs. In this study we explored the feasibility of transdermal delivery of lidocaine across artificial membranes and human skin. Methods Three different nanocarriers were optimised and characterised for lidocaine loading. Permeation of lidocaine was performed with modified, individually calibrated Franz cells across cellulose membrane and eyelid skin. The nanocarriers were polymeric micelles, solid lipid nanoparticles, and self-nanoemulsifying drug delivery systems (SNEDDs) or nanoemulsion. One-way ANOVA with post-hoc Tukey test was used to assess differences in permeation. Findings SNEDD showed the highest lidocaine loading and was the most stable of all nanomedicines. Transmission electron microscope images demonstrated translucency of all suspensions with spherical morphology, which coincided with the measured particle sizes (all <200 nm). The cumulative release of lidocaine-loaded SNEDDs (277 μg/cm² per h) and polymeric micelles (193 μg/cm² per h) were highest across cellulose membrane, and the flux through eyelid skin was the highest with lidocaine-loaded SNEDDs (143 μg/cm² per h) and solid lipid nanoparticles (314 μg/cm² per h).
Published Online February 25, 2016 Poster 31 Moorfields Eye Hospital, London, UK (K Emeriewen MD, Prof G Saleh); and University of Portsmouth, Portsmouth, UK (K Lalatsa PhD) Correspondence to: Dr Krisztina Emeriewen, Moorfields Eye Hospital, South Wing, Bedford MK42 9DJ, UK krisztina.emeriewen@ moorfields.nhs.uk
Interpretation Because of the lipid perturbation effects of human skin, the performance of the nanocarriers was altered compared with the artificial membrane, but overall the achieved flux for all nanomedicines was higher than that previously reported for topical anaesthetic cream tested in skin (73·81 μg/cm² per h for lidocaine hydrochloride and 53·93 μg/cm² per h for prilocaine hydrochloride). We have highlighted the potential capability and use of this mode of drug delivery in eyelid surgery. Funding Royal Society. Contributors KE and GE wrote the abstract. KE and KL conducted the experiments. Declaration of interests We declare no competing interests.
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Feasibility of transdermal delivery of lidocaine-loaded nanocarriers through artificial and human skin: a pilot study Krisztina Emeriewen, Katerina Lalatsa, George Saleh
Abstract Background There is a growing demand for painless, rapid onset, long duration anaesthesia in many aspects of medicine. Nanomedicines (particles 50–500 nm) have shown promise in topical delivery of certain drugs. In this study we explored the feasibility of transdermal delivery of lidocaine across artificial membranes and human skin. Methods Three different nanocarriers were optimised and characterised for lidocaine loading. Permeation of lidocaine was performed with modified, individually calibrated Franz cells across cellulose membrane and eyelid skin. The nanocarriers were polymeric micelles, solid lipid nanoparticles, and self-nanoemulsifying drug delivery systems (SNEDDs) or nanoemulsion. One-way ANOVA with post-hoc Tukey test was used to assess differences in permeation. Findings SNEDD showed the highest lidocaine loading and was the most stable of all nanomedicines. Transmission electron microscope images demonstrated translucency of all suspensions with spherical morphology, which coincided with the measured particle sizes (all <200 nm). The cumulative release of lidocaine-loaded SNEDDs (277 μg/cm² per h) and polymeric micelles (193 μg/cm² per h) were highest across cellulose membrane, and the flux through eyelid skin was the highest with lidocaine-loaded SNEDDs (143 μg/cm² per h) and solid lipid nanoparticles (314 μg/cm² per h).
Published Online February 25, 2016 Poster 31 Moorfields Eye Hospital, London, UK (K Emeriewen MD, Prof G Saleh); and University of Portsmouth, Portsmouth, UK (K Lalatsa PhD) Correspondence to: Dr Krisztina Emeriewen, Moorfields Eye Hospital, South Wing, Bedford MK42 9DJ, UK krisztina.emeriewen@ moorfields.nhs.uk
Interpretation Because of the lipid perturbation effects of human skin, the performance of the nanocarriers was altered compared with the artificial membrane, but overall the achieved flux for all nanomedicines was higher than that previously reported for topical anaesthetic cream tested in skin (73·81 μg/cm² per h for lidocaine hydrochloride and 53·93 μg/cm² per h for prilocaine hydrochloride). We have highlighted the potential capability and use of this mode of drug delivery in eyelid surgery. Funding Royal Society. Contributors KE and GE wrote the abstract. KE and KL conducted the experiments. Declaration of interests We declare no competing interests.
www.thelancet.com
41