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Fibrinolytic activity before and after venous occlusion in thrombotic cases
WEDNESDAY JUNE 27 1990 61
152 Pharmacological data on a new very low molecular weight heparln (OLIGO-H) PAM B, BERGONZINI GL, MXSCELLANI G, BUNCHINI P, OSIUA B OPOCRIN S.p.A. Research Laboratories, CORLO (iUodena), Ita@ Heparin fragments having a low molecular weight of 2,000 D, named OLIGO-H,were prepared by radicalic degradation. Their APTT activity is about 2% of that of heparin, and their AXa chromogenic and coagulometric activity about 30% of that of heparin. Activity on venous thrombosis, determined with the vena cava ligature test, indicates and ED50 lower than 1 mg/kg in case of intravenous administration and a ED50 of 2 m&g in case of subcutaneous administration. In the arterial thrombosis induced by platinum wire, ED50 results to be about 1 mg/kg by i.v. route and
about 5 mg/kg per S.C. route. Collagen-induced platelet aggregation is inhibited not only in vitrobut also ex vivo. The kinetics of effects were studied on the two thrombosis test, in comparison with the blood levels determined by activated factor Xa inhibition test. It is evident that for all the three administration routes (intravenous, subcutaneous, intraileal) there is a dissociation of effects between the kinetics of blood levels of the product and the venous as well as arterial antithrombotic activity.
Von Wlllebrand factor in acute myocardlal lnfarctlon: correlation with lnfarctlon size ANDOLJSEK D, KRALJ J Hematological Clinic, UniversityClinical Center, Ljubljana, Yugoslavia Plasma von Willebrand factor antigen concentrations (vWfAg) have been measured in acute transmural myocardial infarction (AMI) to determine changes in stationary level of vWfAg. In the course of AM1 vWfAg is increased at first, reaching culmination on day 5, and being still higher than normal after 3 weeks. This time course is more pronounced in large AM1 than in small ones. Differences are statistically significant. The time course of vWfAg in these conditions probably reflects primary changes in the function of endothelial cells in the damaged myocardium and the secondary changes in the function of other endothelial cells due to mediators released from damaged myocardium.
Fibrlnolytic activity before and after venous occlusion in thrombotic cases ZAMBOULAKISJ, TSOUKALA C, LIAPI S, MANDALAKI T 2”dReg. Blood Transfusion Centre-Haemostasis Lab., Laikon Hospital Athens, Athens, Greece ECLT (euglobulin clot lysis time) and t-PA activity before and after venous occlusion (VO) were studied in patients with thromboembolic episodes and in 8 surgeons. A group of 15 healthy subjects was used for establishment of reference values. ECLT and t-PA assay were performed according to a standardized technique (Verheijen et al., Thromb. Haemostas. 1982; 48: 266-9) of the ECAT study. Patients: 47 patients with thromboembolic episodes aged 18-72 yrs (male: 27, female: 20) with a history of deep vein thrombosis (DVT): 19, pulmonary embolism (PE): 14, Superficial thrombophlebitis migrans (STM): 10, myocardial infarction
(MI): 4. Surgeons: 8, aged 28-30 yrs. Results: The difference in t-PA values observed between the thrombotic patients and the control group was not statistically significant (nss) (p>O.l) whereas the differences in ECLT values, both before and after VO, were statistically significant (ss) (p
152 Pharmacological data on a new very low molecular weight heparln (OLIGO-H) PAM B, BERGONZINI GL, MXSCELLANI G, BUNCHINI P, OSIUA B OPOCRIN S.p.A. Research Laboratories, CORLO (iUodena), Ita@ Heparin fragments having a low molecular weight of 2,000 D, named OLIGO-H,were prepared by radicalic degradation. Their APTT activity is about 2% of that of heparin, and their AXa chromogenic and coagulometric activity about 30% of that of heparin. Activity on venous thrombosis, determined with the vena cava ligature test, indicates and ED50 lower than 1 mg/kg in case of intravenous administration and a ED50 of 2 m&g in case of subcutaneous administration. In the arterial thrombosis induced by platinum wire, ED50 results to be about 1 mg/kg by i.v. route and
about 5 mg/kg per S.C. route. Collagen-induced platelet aggregation is inhibited not only in vitrobut also ex vivo. The kinetics of effects were studied on the two thrombosis test, in comparison with the blood levels determined by activated factor Xa inhibition test. It is evident that for all the three administration routes (intravenous, subcutaneous, intraileal) there is a dissociation of effects between the kinetics of blood levels of the product and the venous as well as arterial antithrombotic activity.
Von Wlllebrand factor in acute myocardlal lnfarctlon: correlation with lnfarctlon size ANDOLJSEK D, KRALJ J Hematological Clinic, UniversityClinical Center, Ljubljana, Yugoslavia Plasma von Willebrand factor antigen concentrations (vWfAg) have been measured in acute transmural myocardial infarction (AMI) to determine changes in stationary level of vWfAg. In the course of AM1 vWfAg is increased at first, reaching culmination on day 5, and being still higher than normal after 3 weeks. This time course is more pronounced in large AM1 than in small ones. Differences are statistically significant. The time course of vWfAg in these conditions probably reflects primary changes in the function of endothelial cells in the damaged myocardium and the secondary changes in the function of other endothelial cells due to mediators released from damaged myocardium.
Fibrlnolytic activity before and after venous occlusion in thrombotic cases ZAMBOULAKISJ, TSOUKALA C, LIAPI S, MANDALAKI T 2”dReg. Blood Transfusion Centre-Haemostasis Lab., Laikon Hospital Athens, Athens, Greece ECLT (euglobulin clot lysis time) and t-PA activity before and after venous occlusion (VO) were studied in patients with thromboembolic episodes and in 8 surgeons. A group of 15 healthy subjects was used for establishment of reference values. ECLT and t-PA assay were performed according to a standardized technique (Verheijen et al., Thromb. Haemostas. 1982; 48: 266-9) of the ECAT study. Patients: 47 patients with thromboembolic episodes aged 18-72 yrs (male: 27, female: 20) with a history of deep vein thrombosis (DVT): 19, pulmonary embolism (PE): 14, Superficial thrombophlebitis migrans (STM): 10, myocardial infarction
(MI): 4. Surgeons: 8, aged 28-30 yrs. Results: The difference in t-PA values observed between the thrombotic patients and the control group was not statistically significant (nss) (p>O.l) whereas the differences in ECLT values, both before and after VO, were statistically significant (ss) (p