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Fibroblasts Specifically Affect Proteoglycan Expression in Different Prostate Cancer Cells
Annals of Oncology 25 (Supplement 4): iv564–iv573, 2014 doi:10.1093/annonc/mdu359.24
tumour biology and pathology 1653P
FIBROBLASTS SPECIFICALLY AFFECT PROTEOGLYCAN EXPRESSION IN DIFFERENT PROSTATE CANCER CELLS
abstracts
Aim: Over time, the study of malignant tumours has concentrated on cancer cells only. However, the important role of the cancer cell microenvironment and cell-extracellular matrix (ECM) interactions is becoming evident. One of the main components of ECM is proteoglycans (PGs) - complex glycosylated molecules, which are expressed on cell surface and in extracellular matrix, and play an important role in cell-cell and cell-matrix interactions and signaling. The aim of our work was to investigate an effect of fibroblasts on proteoglycans expression in morphologically different prostate cancer cells.
© European Society for Medical Oncology 2014. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: [email protected].
Downloaded from https://academic.oup.com/annonc/article-abstract/25/suppl_4/iv570/2242331 by guest on 24 October 2019
A.V. Suhovskih1, E.V. Grigorieva2 1 Department of Natural Science, Novosibirsk State University, Novosibirsk, RUSSIAN FEDERATION 2 Laboratory of Molecular Mechanisms of Carcinogenesis, Institute of Molecular Biology and Biophysics, Novosibirsk, RUSSIAN FEDERATION
Methods: Normal human fibroblasts were co-cultured with normal (PNT2) and prostate cancer cells (PC3, LNCaP, DU145) in cells co-culture model in vitro. Expression and localisation of main proteoglycans (Synd-1, Gly-1, Perl, Vers, Brev, NG2, CD44, Sergl, Dec, Agg, Bigl, Lum) were determined in the cell lines before and after magnetic separation of the cells using real-time RT-PCR analysis and immunocytochemical staining. Results: It was shown that co-cultivation with stromal fibroblasts downregulates PGs expression in normal PNT2 cells at total expression level and change their expression pattern. Androgen-dependent non-metastatic LNCaP cells had similar PGs expression level but their response to the fibroblast influence was distorted. Androgen-independent metastatic PC3 and DU145 cells were characterized by significant decrease of PGs expression and the cells were not able to react to the fibroblast influence. However, in spite of the shown similar effects of the fibroblasts to PC3 and DU145 prostate cancer cells at mRNA level, the cell lines revealed differential reaction to fibroblasts at the protein level. Conclusions: Collectively, obtained data show a different ability of aggressive and non-aggresive prostate cancer cells to respond to the stromal fibroblasts, supporting an important role of ECM in prostate carcinogenesis and suggesting proteoglycans as possible microenviromental biomarkers for prostate tumour diagnosis and treatment. Disclosure: All authors have declared no conflicts of interest.
tumour biology and pathology 1653P
FIBROBLASTS SPECIFICALLY AFFECT PROTEOGLYCAN EXPRESSION IN DIFFERENT PROSTATE CANCER CELLS
abstracts
Aim: Over time, the study of malignant tumours has concentrated on cancer cells only. However, the important role of the cancer cell microenvironment and cell-extracellular matrix (ECM) interactions is becoming evident. One of the main components of ECM is proteoglycans (PGs) - complex glycosylated molecules, which are expressed on cell surface and in extracellular matrix, and play an important role in cell-cell and cell-matrix interactions and signaling. The aim of our work was to investigate an effect of fibroblasts on proteoglycans expression in morphologically different prostate cancer cells.
© European Society for Medical Oncology 2014. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: [email protected].
Downloaded from https://academic.oup.com/annonc/article-abstract/25/suppl_4/iv570/2242331 by guest on 24 October 2019
A.V. Suhovskih1, E.V. Grigorieva2 1 Department of Natural Science, Novosibirsk State University, Novosibirsk, RUSSIAN FEDERATION 2 Laboratory of Molecular Mechanisms of Carcinogenesis, Institute of Molecular Biology and Biophysics, Novosibirsk, RUSSIAN FEDERATION
Methods: Normal human fibroblasts were co-cultured with normal (PNT2) and prostate cancer cells (PC3, LNCaP, DU145) in cells co-culture model in vitro. Expression and localisation of main proteoglycans (Synd-1, Gly-1, Perl, Vers, Brev, NG2, CD44, Sergl, Dec, Agg, Bigl, Lum) were determined in the cell lines before and after magnetic separation of the cells using real-time RT-PCR analysis and immunocytochemical staining. Results: It was shown that co-cultivation with stromal fibroblasts downregulates PGs expression in normal PNT2 cells at total expression level and change their expression pattern. Androgen-dependent non-metastatic LNCaP cells had similar PGs expression level but their response to the fibroblast influence was distorted. Androgen-independent metastatic PC3 and DU145 cells were characterized by significant decrease of PGs expression and the cells were not able to react to the fibroblast influence. However, in spite of the shown similar effects of the fibroblasts to PC3 and DU145 prostate cancer cells at mRNA level, the cell lines revealed differential reaction to fibroblasts at the protein level. Conclusions: Collectively, obtained data show a different ability of aggressive and non-aggresive prostate cancer cells to respond to the stromal fibroblasts, supporting an important role of ECM in prostate carcinogenesis and suggesting proteoglycans as possible microenviromental biomarkers for prostate tumour diagnosis and treatment. Disclosure: All authors have declared no conflicts of interest.