- Email: [email protected]
Fine structure and cellular contacts of Langerhans cells in psoriasis
Posters - Dertnatopathology
IPi39 I? de Spain
Clear-cell
syringoma
Pablo, R. Suarez, I? Ortega. General
Hospital,
Segovia,
Clear-cell syringoma is a rare histologic variant of syringome that is otherwise clinically indistinguishable from ordinary syringoma except a frequent coexistence with diabetes mellitus. Less than 40 cases have been reported, and noneone exclusivelly on cheeks. A 63-year-old woman ahd many asymptomatic papules on her cheeks. These papules had graduallyd eveloped over the past two years. She had no remarkable medical history. On physical examination there were multiple 5 to IO mm erythematous, indurated papulonodules on both cheeks, no affecting eyelids. Laboratory examination revealed no abnormal findings. Examination of a biopsy specimen revealed a preponderance of clear cells arranged in ductal and epithelial cords, some with tadpole morphology dispersed in a dense fibrous strome. The lumina of the ducts contained amorphous material. The clear cells were characterized by darkly basophilic, slightly eccentric nuclei. The PAS stain was strongly positive prior diastase digestion but was negative after exposure to diastase. We emphasized the rareness of the location and the unusual histologic findings. lymphadenoma I P140 Cutaneous P de Pablo, P Ortega, R. Gilt, C. Ballestin’. General Segovia;
’ University
Hospital
“12 Octubre”,
Hospital, Spain
Madrid,
An unusual cutaneous neoplasm characterized by a proliferation of basaloid epithelial cells with intraepithelial lymphcytes and a dense desmoplastic stroma was reported in 1991 by Santa Cruz el al under the descriptive term of cutaneous lymphadenoma. Herein we present a 42-year-old man who was seen for evaluation of a asymtomatic 7 mm nodule on his cheek of ten years’ evolution. Examination of the skin specimen showed a well circumscribed nodule with scant epidermal connections. The proliferation consisted of multiple, rounded lobules of basaloid cells with some degree of pheripheral palisading immersed in a dense fibrous strome. There was an intense infiltrate of small lymphocytes within the tumor lobules with some spilage into the strome. Rare isolated cells showing apparent sebaceous differentiation within the tumor lobules was noted. The immunohistochemical findings reflected the diverse composition of this tumor. In this presentation, we summarize the reported cases. I PI41
Angiolymphoid hyperplasia with eosinophilia epitheloid haemangioma
J. Jautovdt, A. Boor?, D. JarMkovat, ‘University Hospital,
Hospital, Pathology
Dertnatology Clinic, KoSice,
K. BeneEova’. Clinic, KoSice; Slovakia
2Uttiversity
The authors draw attention to the need of histopathological examination of epitheloid haemangioma, if the clinical morphology is not obvious. They describe a patient with epitheloid haemangioma on the left car lobe which was totally extirpated
s219
at the Plastic Surgery Department with subsequent histopathological examination. The patient is now two and a half years after total extirpation of an epitheloid haemangioma, he is in clinical remission, in good health without relapses. Based on data in the literature as well as their own experience the authors consider as the best therapeutic method surgical removal of lesiions, after which relapses do not occur. I P142
Fine structure and cellular contacts Langerhans cells in psoriasis
of
G. Gzttlrk’, S. Erboz’, S. Kara9ah2, R. Deveci’, 0. Devec?. ‘Dept. of dertnatology, tnedicalfac.; 2dept. fat., Vttiversiry of Ege, iz.tnir~ Tiirkiye
of biology,
science
Skin biopsy specimens taken from ten patients with psoriasis were investigated by electron microscopy. The most consistent finding in the lesional skin was the widening of intercellular spaces. Two different Langerhans cell (LC) types were identified considering the morphology of the cell organels and density of granules. In the first type of LCs endoplasmic reticulum (ER) membranes were appeared as very enlarged vacuoles. The number of LC granules was decrease. In the second type of the LCs, the membranes of ER were in the form of narrow cannals. Golgi areas were well developed. There were numerous rod-like granules and paracristalline formations in various morphology and sizes. In spite of the wide spaces, there were contact points where glycocalixes interfered between the membranes of LC and keratinocyte (K) and also between the membranes of K and lymphocyte. Observations of mentioned contacts and cellular morphological changes support a functional interaction among these cells. ElP143
Fine structure and cellular Langerhans cells in lichen
contacts planus
of
G. bzttlrk’, S. Erboz’, G. Atilla’, N. Bt9akc1’, S. Kara9ah3, R. Deveci3, 6. Deveci3. ‘Dept. of Dertnatology, tnedicalfac.; ‘dept. ofperiodontology, science fat. University
dentisttyfac.; of Ege, hnir;
3dept. Tiirkiye
of biology,
Skin and oral mucosa biopsy specimens taken from eleven patients with lichen planus were investigated by electron microscopy. In lesional skin, the cytoplasm of Langerhans cells (LCs) was widened. LC granules were smaller and narrower. Number of the granules in the cytoplasmic extensions was increased. A number of close facing places were observed between the LCs and other cell types, and their glycocalyxes were interfered with each other. Additionally, direct cytoplasmic continuity between LC-Keratinocyte (K), LC-Lymphocyte (L) and also L-K membranes were observed indicating a close functional interaction among the the mentioned cells. LC-L were agregated within the Pautrier microabse-like structures. Close contact points were observed between the facing membmnes of the cells in this structure. Virus-like particles were found in some of the kemtinocyte facing to LCs.
IPi39 I? de Spain
Clear-cell
syringoma
Pablo, R. Suarez, I? Ortega. General
Hospital,
Segovia,
Clear-cell syringoma is a rare histologic variant of syringome that is otherwise clinically indistinguishable from ordinary syringoma except a frequent coexistence with diabetes mellitus. Less than 40 cases have been reported, and noneone exclusivelly on cheeks. A 63-year-old woman ahd many asymptomatic papules on her cheeks. These papules had graduallyd eveloped over the past two years. She had no remarkable medical history. On physical examination there were multiple 5 to IO mm erythematous, indurated papulonodules on both cheeks, no affecting eyelids. Laboratory examination revealed no abnormal findings. Examination of a biopsy specimen revealed a preponderance of clear cells arranged in ductal and epithelial cords, some with tadpole morphology dispersed in a dense fibrous strome. The lumina of the ducts contained amorphous material. The clear cells were characterized by darkly basophilic, slightly eccentric nuclei. The PAS stain was strongly positive prior diastase digestion but was negative after exposure to diastase. We emphasized the rareness of the location and the unusual histologic findings. lymphadenoma I P140 Cutaneous P de Pablo, P Ortega, R. Gilt, C. Ballestin’. General Segovia;
’ University
Hospital
“12 Octubre”,
Hospital, Spain
Madrid,
An unusual cutaneous neoplasm characterized by a proliferation of basaloid epithelial cells with intraepithelial lymphcytes and a dense desmoplastic stroma was reported in 1991 by Santa Cruz el al under the descriptive term of cutaneous lymphadenoma. Herein we present a 42-year-old man who was seen for evaluation of a asymtomatic 7 mm nodule on his cheek of ten years’ evolution. Examination of the skin specimen showed a well circumscribed nodule with scant epidermal connections. The proliferation consisted of multiple, rounded lobules of basaloid cells with some degree of pheripheral palisading immersed in a dense fibrous strome. There was an intense infiltrate of small lymphocytes within the tumor lobules with some spilage into the strome. Rare isolated cells showing apparent sebaceous differentiation within the tumor lobules was noted. The immunohistochemical findings reflected the diverse composition of this tumor. In this presentation, we summarize the reported cases. I PI41
Angiolymphoid hyperplasia with eosinophilia epitheloid haemangioma
J. Jautovdt, A. Boor?, D. JarMkovat, ‘University Hospital,
Hospital, Pathology
Dertnatology Clinic, KoSice,
K. BeneEova’. Clinic, KoSice; Slovakia
2Uttiversity
The authors draw attention to the need of histopathological examination of epitheloid haemangioma, if the clinical morphology is not obvious. They describe a patient with epitheloid haemangioma on the left car lobe which was totally extirpated
s219
at the Plastic Surgery Department with subsequent histopathological examination. The patient is now two and a half years after total extirpation of an epitheloid haemangioma, he is in clinical remission, in good health without relapses. Based on data in the literature as well as their own experience the authors consider as the best therapeutic method surgical removal of lesiions, after which relapses do not occur. I P142
Fine structure and cellular contacts Langerhans cells in psoriasis
of
G. Gzttlrk’, S. Erboz’, S. Kara9ah2, R. Deveci’, 0. Devec?. ‘Dept. of dertnatology, tnedicalfac.; 2dept. fat., Vttiversiry of Ege, iz.tnir~ Tiirkiye
of biology,
science
Skin biopsy specimens taken from ten patients with psoriasis were investigated by electron microscopy. The most consistent finding in the lesional skin was the widening of intercellular spaces. Two different Langerhans cell (LC) types were identified considering the morphology of the cell organels and density of granules. In the first type of LCs endoplasmic reticulum (ER) membranes were appeared as very enlarged vacuoles. The number of LC granules was decrease. In the second type of the LCs, the membranes of ER were in the form of narrow cannals. Golgi areas were well developed. There were numerous rod-like granules and paracristalline formations in various morphology and sizes. In spite of the wide spaces, there were contact points where glycocalixes interfered between the membranes of LC and keratinocyte (K) and also between the membranes of K and lymphocyte. Observations of mentioned contacts and cellular morphological changes support a functional interaction among these cells. ElP143
Fine structure and cellular Langerhans cells in lichen
contacts planus
of
G. bzttlrk’, S. Erboz’, G. Atilla’, N. Bt9akc1’, S. Kara9ah3, R. Deveci3, 6. Deveci3. ‘Dept. of Dertnatology, tnedicalfac.; ‘dept. ofperiodontology, science fat. University
dentisttyfac.; of Ege, hnir;
3dept. Tiirkiye
of biology,
Skin and oral mucosa biopsy specimens taken from eleven patients with lichen planus were investigated by electron microscopy. In lesional skin, the cytoplasm of Langerhans cells (LCs) was widened. LC granules were smaller and narrower. Number of the granules in the cytoplasmic extensions was increased. A number of close facing places were observed between the LCs and other cell types, and their glycocalyxes were interfered with each other. Additionally, direct cytoplasmic continuity between LC-Keratinocyte (K), LC-Lymphocyte (L) and also L-K membranes were observed indicating a close functional interaction among the the mentioned cells. LC-L were agregated within the Pautrier microabse-like structures. Close contact points were observed between the facing membmnes of the cells in this structure. Virus-like particles were found in some of the kemtinocyte facing to LCs.