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Flow cytometry and sorting
360
Journal of Immunological Methods, 35 (1980) 360--361
© Elsevier/North-Holland Biomedical Press
B o o k reviews
Flow C y t o m e t r y and Sorting, edited by M.R. Melamed, P.F. Mullaney and M.L. Mendelsohn. Wiley and Sons, Chichester, 1979 (716 pp., illus.) £ 39.50. Flow c y t o m e t r y , a technique originally designed for counting and sizing solid particles in liquid media, has developed in the past 20 years into a tool for rapidly quantitating physico-chemical properties of living cells and for sorting cells according to their properties. The availability of improved, sophisticated instrumentation enabled the application of this technique in different fields of biology and medicine. The above monograph, as stated in the editors' preface, aims to present in a single volume the knowledge of this subject that has been thus far dispersed in various engineering, physical and biomedical journals, symposia and patents. This multi-author b o o k is divided into 10 parts comprising a total of 41 chapters. After an historical introduction to the subject h y d r o d y n a m i c principles of the laminar flow system are explained and several types of flow cytometric instruments described, based on the electrical resistance pulse sizing, light scattering and fluorescence measurements, including flow sorters for separation of cells with defined characteristics. The procedures used in cell preparation prerequisite for flow c y t o m e t r y are the subject of part 3. Special techniques for quantitative staining of cellular DNA and enzymes as well as the use of plastic microspheres as antibody labels in identifying cells bearing certain surface antigens are described in part 4. Part 5 contains a discussion of reference standards for flow c y t o m e t r y and a description of methods and instruments used in data processing. Parts 6--9 deal with the applications of flow c y t o m e t r y in cell biology, immunology (including cytochemical characterization of granulocytes, m o n o c y t e s and lymphocytes, and the analysis and sorting of cells with different membrane antigenic markers), haematology (leukocytic enzyme analysis and differential leukocyte count), and oncology. Part 10 contains a review of light sources, detectors and flow chambers, and a detailed description of commercially available instruments including research and clinical cytometers, cell sorters, and two automatic systems. The text is provided with bibliographic references and a comprehensive subject index, and illustrated with numerous diagrams and photographs. A novel and useful feature are the summaries of all the chapters presented in the second part of introduction; the technical data for available instruments are summarized in tabular form. The b o o k is printed on high-quality paper and has an aesthetic appearance. Its large format results from the circumstance that it covers material sufficient to fill several medium-size
361 volumes. It should prove very useful to those working with flow cytometric techniques and also to others who may wish to become acquainted with them. F. Borek
The Molecular Basis of Immune Cell Function, edited by J. Gordin Kaplan. Elsevier/North-Holland, Amsterdam, 1979 (781 pp., illus). This volume contains the proceedings of the 13th International Leukocyte Culture Conference held in Ottawa, Canada, in May 1979. Its contents include major presentations (partly having the character of reviews), communications, and workshop reports. The papers are grouped according to themes such as membrane biochemistry, Fc receptors, cytoskeleton, cations, enzymes, complement, mitogenic activation and proliferation, soluble factors and mediators, differentiation, cell-cell interactions, lymphocyte subpopulations, macrophages, histocompatibility antigens, cytotoxic reactions, tumor immunology, and clinical autoimmunity. In the introductory chapter the book's editor traces the history of research on leukocytes in vitro since the discovery of mitogen-induced blastogenesis in 1959 and of the Leukocyte Culture Conferences since their inception in 1965; in the author's opinion the activated lymphocyte can serve as a model for the study of cell proliferation in general. Further, among the topics of major presentations one might mention the regulation of lymphocyte function by anti-idiotype antibodies, tolerance as a probe for age
Journal of Immunological Methods, 35 (1980) 360--361
© Elsevier/North-Holland Biomedical Press
B o o k reviews
Flow C y t o m e t r y and Sorting, edited by M.R. Melamed, P.F. Mullaney and M.L. Mendelsohn. Wiley and Sons, Chichester, 1979 (716 pp., illus.) £ 39.50. Flow c y t o m e t r y , a technique originally designed for counting and sizing solid particles in liquid media, has developed in the past 20 years into a tool for rapidly quantitating physico-chemical properties of living cells and for sorting cells according to their properties. The availability of improved, sophisticated instrumentation enabled the application of this technique in different fields of biology and medicine. The above monograph, as stated in the editors' preface, aims to present in a single volume the knowledge of this subject that has been thus far dispersed in various engineering, physical and biomedical journals, symposia and patents. This multi-author b o o k is divided into 10 parts comprising a total of 41 chapters. After an historical introduction to the subject h y d r o d y n a m i c principles of the laminar flow system are explained and several types of flow cytometric instruments described, based on the electrical resistance pulse sizing, light scattering and fluorescence measurements, including flow sorters for separation of cells with defined characteristics. The procedures used in cell preparation prerequisite for flow c y t o m e t r y are the subject of part 3. Special techniques for quantitative staining of cellular DNA and enzymes as well as the use of plastic microspheres as antibody labels in identifying cells bearing certain surface antigens are described in part 4. Part 5 contains a discussion of reference standards for flow c y t o m e t r y and a description of methods and instruments used in data processing. Parts 6--9 deal with the applications of flow c y t o m e t r y in cell biology, immunology (including cytochemical characterization of granulocytes, m o n o c y t e s and lymphocytes, and the analysis and sorting of cells with different membrane antigenic markers), haematology (leukocytic enzyme analysis and differential leukocyte count), and oncology. Part 10 contains a review of light sources, detectors and flow chambers, and a detailed description of commercially available instruments including research and clinical cytometers, cell sorters, and two automatic systems. The text is provided with bibliographic references and a comprehensive subject index, and illustrated with numerous diagrams and photographs. A novel and useful feature are the summaries of all the chapters presented in the second part of introduction; the technical data for available instruments are summarized in tabular form. The b o o k is printed on high-quality paper and has an aesthetic appearance. Its large format results from the circumstance that it covers material sufficient to fill several medium-size
361 volumes. It should prove very useful to those working with flow cytometric techniques and also to others who may wish to become acquainted with them. F. Borek
The Molecular Basis of Immune Cell Function, edited by J. Gordin Kaplan. Elsevier/North-Holland, Amsterdam, 1979 (781 pp., illus). This volume contains the proceedings of the 13th International Leukocyte Culture Conference held in Ottawa, Canada, in May 1979. Its contents include major presentations (partly having the character of reviews), communications, and workshop reports. The papers are grouped according to themes such as membrane biochemistry, Fc receptors, cytoskeleton, cations, enzymes, complement, mitogenic activation and proliferation, soluble factors and mediators, differentiation, cell-cell interactions, lymphocyte subpopulations, macrophages, histocompatibility antigens, cytotoxic reactions, tumor immunology, and clinical autoimmunity. In the introductory chapter the book's editor traces the history of research on leukocytes in vitro since the discovery of mitogen-induced blastogenesis in 1959 and of the Leukocyte Culture Conferences since their inception in 1965; in the author's opinion the activated lymphocyte can serve as a model for the study of cell proliferation in general. Further, among the topics of major presentations one might mention the regulation of lymphocyte function by anti-idiotype antibodies, tolerance as a probe for age