Fluorescein Angiograph Patterns in the Wet Macula

VOL. 78, NO. 5

test was performed on all graft recipients in the fourth postoperative week. T h e chal­ lenging antigen used in these tests was an homogenate of pooled bovine corneas. Clinically and histologically each rabbit showed typical corneal graft reaction. T h e leukocyte migration inhibition and skin test were positive. However, the H 3 -thymidine incorporation test was unable to detect spe­ cific systemic sensitization. From the overall results of this experi­ ment we concluded that bovine corneal xenograft can induce systemic sensitization in rabbits. FLUORESCEIN

ANGIOGRAPH

IN THE W E T

PATTERNS

(Toronto)

Analysis of the fluorescein angiographs of 100 patients referred for examination be­ cause of macular edema revealed fluorescein leakage patterns in four well-defined groups: pattern 1, single discrete leak; pattern 2, multiple discrete leaks; pattern 3, diffuse leakage, and pattern 4, "flower-petal" leak­ age. Thirty-two patients volunteered to have bilateral fluorescein angiography repeated after a period ranging from 24 to 51 months. Patients in pattern 1 maintained good vision, but developed pattern 2 leak­ age. Patients in pattern 2 maintained good vision and unchanged leakage patterns, but most developed leakage in the asymptomatic eye. Patients in pattern 3 lost considerable vision in both eyes, associated with diffuse leakage, despite photocoagulation. Pattern 4 leakage seemed benign. Close fluorescein follow-up in all patients with macular leaks is essential. HLSTOCHEMICAL LOCALIZATION OF LYSOENZYMES GRAFT

IN

CORNEAL

REACTION

S. M. H a s a n y and P . K. Basu

T w o enzymes—B-glucuronidase and acid phosphatase were used as the markers of lysosomal hydrolytic enzymes. These were traced histochemically at various times be­ fore and after the onset of graft reaction in a corneal xenograft model. Results showed a relationship between the degree of graft opacity, cellular infiltration, and enzyme localization. It appeared that during the early stages of graft reaction the granulocytes, and during the late stages, the agranulocytes were the main source of the enzymes. ROLE

OF

NEUTROPHILS GRAFT

IN

CORNEAL

REACTION

N . S. Ranadive and P . K. Basu ( T o r o n t o )

MACULA

J. S. Wise and C. B. Mortimer

SOMAL

865

MEETINGS, CONFERENCES, SYMPOSIA

W h e n neutrophils interact with immune complexes they release a specific cationic protein, viz., band 2 protein. O u r objective was to determine whether band 2 protein could be detected in the re­ acting host cornea during a corneal xeno­ graft response. T h e host corneas containing the graft were harvested at different times before and after the onset of corneal graft reaction. Each was homogenized and processed appro­ priately for identifying the band 2 protein electrophoretically. T h e band 2 protein was detectable in the host cornea from 6 to 20 days postoperatively, roughly the time at which histological studies showed an accumulation of neu­ trophils around the grafted tissue. Severity of the graft reaction in this period ap­ peared to run parallel to the amount of free band 2 protein. A t the advanced stage of graft reaction, however, although large numbers of granulocytes were present in the graft bed, band 2 protein was not de­ tected. HUMAN

(Toronto)

T h e objective of this experiment was to localize hydrolytic enzymes in the graft bed during corneal graft reaction.

EYE

P . E . Hallett

MOVEMENTS

(Toronto)

T h e eye captures only 6 0 % of 17-30 min of arc target steps with a single saccade (according to our technique), but this does