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Fluorescein Angiography of the Anterior Segment
FLUORESCEIN ANGIOGRAPHY O F T H E ANTERIOR SEGMENT J. BRUUN-JENSEN,
M.D.
Aarhus, Denmark Following the description by Novotny and Alvis 1 of a simple technique for serial fluorescein photography of the retina, many workers studied the pathology of the retinal From the Department of Ophthalmology, Kommunehospitalet, Aarhus University School of Medi cine. A grant from the Carl and Ellen Hertz Foun dation made this study possible.
vessels. There is, however, only one report on the use of this technique for examination of the anterior segment of the eye; Jensen and Lundbaek 2 used a Zeiss fundus camera with a special lens for fluorescein angiography of the iris. In order to benefit from the advantages the slitlamp offers in fluorescein angiog-
Fig. 1 (Bruun-Jensen). The Zeiss photo-slitlamp modified for fluorescein angiography. (1) Automatic recording unit. (2) Robot motor-camera. (3) Special holder for blue filter and concave lens. (4) Plastic tube in the air-cooling system. (5) Ignition unit engaged to high-speed flash generator. (6) Variable transformer. 842
VOL. 67, NO. 6
FLUORESCEIN ANGIOGRAPHY
843
Fig. 2 (Bruun-Jensen). The iris of a normal 27-year-old woman 24 seconds after injection of fluorescein.
raphy, I made a number of modifications in a Zeiss photo-slitlamp. It was equipped with a Robot motor-camera and absorption filters and then attached to a high-speed flash gen erator. Changes (fig. 1) included: The lighting section. The original power supply and flash generator were dismantled. Removal from the lamphouse of the cap con taining the high-tension cable and the mounting screw made it possible to mount the ignition unit from the Zeiss fundus camera on the flash tube of the photo-slitlamp and to connect the high-speed flash genera tor. A variable transformer supplied power to the electric bulb. The flash tube was cooled by air-ducts in the lamp house and by use of a plastic tube
to connect an ordinary vacuum cleaner to the lamp-house. A blue filter (Schott B.G. 12/0.7 mm) in a specially designed holder to permit swing ing back and forth was placed, together with a concave lens, in front of the light aperature. The concave lens was used (—7.5 diop ters) because the lighting field was too small to photograph the entire iris. Photographing section. A division ring engaged the Robot motor-camera to the ob jective of the photo-slitlamp. To obtain suf ficient space for the camera, the double ocular of the microscope was turned horizontally 180 degrees. A special arm fastened to the anterior part of the microscope held the auto matic recording unit. Finally, an emission fil-
VOL. 67, NO. 6
FLUORESCEIN ANGIOGRAPHY
845
Fig. 3 (Bruun-Jensen). The anterior chamber angle of a 57-year-old diabetic woman who had previously undergone cataract surgery. The picture was taken 18 seconds after injection of fluorescein.
ter (Schott G.G. 14/3 m m ) was placed in the division ring in front of the camera. Ilford H . P . 4 (36 exposures), the film regularly utilized, suffered about 2 5 % over development in Microphen ( I l f o r d ) . Immediately prior to photography, 5.0 ml of a 1 0 % solution of fluorescein natrium were injected into a superficial vein in the subject's forearm. F o r fluorescein angiography of the iris, a 60-degree light incidence was used to elimi nate undesirable light reflexes from the pu pillary a r e a ; simultaneously, it brought the iris structure into relief, thereby making possible better depth in the finished pictures. Figure 2 was made 24 seconds after fluores cein injection of a normal 27-year-old woman. A one-mirror Goldmann contact lens was used to examine the anterior chamber angle. A 57-year-old diabetic woman, previously undergoing cataract surgery, was the subject for Figure 3, made 18 seconds after fluores cein injection. The contact lens was also used to examine
"T
the ciliary processes by fluorescein photogra phy through the colobomas of the iris and iris dialyses. Figure 4, taken 14 minutes after fluorescein injection, shows the ciliary process of a 24-year-old man with a large traumatic iridodialysis. SUMMARY
A Zeiss photo-slitlamp was modified for fluorescein angiography of the anterior seg ment. Changes included an air-cooled lighting section together with absorption filters and a concave lens. A Robot motor-camera w a s used and light was directed to the iris at a 60-degree angle. T h e anterior chamber angle and the ciliary processes were photographed through a one-mirror Goldmann contact lens. Smedegade
6 (4200) Slagelse,
Denmark
REFERENCES
1. Novotny, H. R. and Alvis, D. L. : A method of photographing fluorescence in circulating blood in the human retina. Circulation 24:82, 1961. 2. Jensen, V. A. and Lundbaek, K. : Fluorescence angiography of the iris in recent and long-term di abetes. Diabetologica 4:161, 1968.
WTO
Fig. 4 (Bruun-Jensen). The ciliary process of a 24-year-old man with a traumatic iridodialysis, about 14 minutes after fluorescein injection.
M.D.
Aarhus, Denmark Following the description by Novotny and Alvis 1 of a simple technique for serial fluorescein photography of the retina, many workers studied the pathology of the retinal From the Department of Ophthalmology, Kommunehospitalet, Aarhus University School of Medi cine. A grant from the Carl and Ellen Hertz Foun dation made this study possible.
vessels. There is, however, only one report on the use of this technique for examination of the anterior segment of the eye; Jensen and Lundbaek 2 used a Zeiss fundus camera with a special lens for fluorescein angiography of the iris. In order to benefit from the advantages the slitlamp offers in fluorescein angiog-
Fig. 1 (Bruun-Jensen). The Zeiss photo-slitlamp modified for fluorescein angiography. (1) Automatic recording unit. (2) Robot motor-camera. (3) Special holder for blue filter and concave lens. (4) Plastic tube in the air-cooling system. (5) Ignition unit engaged to high-speed flash generator. (6) Variable transformer. 842
VOL. 67, NO. 6
FLUORESCEIN ANGIOGRAPHY
843
Fig. 2 (Bruun-Jensen). The iris of a normal 27-year-old woman 24 seconds after injection of fluorescein.
raphy, I made a number of modifications in a Zeiss photo-slitlamp. It was equipped with a Robot motor-camera and absorption filters and then attached to a high-speed flash gen erator. Changes (fig. 1) included: The lighting section. The original power supply and flash generator were dismantled. Removal from the lamphouse of the cap con taining the high-tension cable and the mounting screw made it possible to mount the ignition unit from the Zeiss fundus camera on the flash tube of the photo-slitlamp and to connect the high-speed flash genera tor. A variable transformer supplied power to the electric bulb. The flash tube was cooled by air-ducts in the lamp house and by use of a plastic tube
to connect an ordinary vacuum cleaner to the lamp-house. A blue filter (Schott B.G. 12/0.7 mm) in a specially designed holder to permit swing ing back and forth was placed, together with a concave lens, in front of the light aperature. The concave lens was used (—7.5 diop ters) because the lighting field was too small to photograph the entire iris. Photographing section. A division ring engaged the Robot motor-camera to the ob jective of the photo-slitlamp. To obtain suf ficient space for the camera, the double ocular of the microscope was turned horizontally 180 degrees. A special arm fastened to the anterior part of the microscope held the auto matic recording unit. Finally, an emission fil-
VOL. 67, NO. 6
FLUORESCEIN ANGIOGRAPHY
845
Fig. 3 (Bruun-Jensen). The anterior chamber angle of a 57-year-old diabetic woman who had previously undergone cataract surgery. The picture was taken 18 seconds after injection of fluorescein.
ter (Schott G.G. 14/3 m m ) was placed in the division ring in front of the camera. Ilford H . P . 4 (36 exposures), the film regularly utilized, suffered about 2 5 % over development in Microphen ( I l f o r d ) . Immediately prior to photography, 5.0 ml of a 1 0 % solution of fluorescein natrium were injected into a superficial vein in the subject's forearm. F o r fluorescein angiography of the iris, a 60-degree light incidence was used to elimi nate undesirable light reflexes from the pu pillary a r e a ; simultaneously, it brought the iris structure into relief, thereby making possible better depth in the finished pictures. Figure 2 was made 24 seconds after fluores cein injection of a normal 27-year-old woman. A one-mirror Goldmann contact lens was used to examine the anterior chamber angle. A 57-year-old diabetic woman, previously undergoing cataract surgery, was the subject for Figure 3, made 18 seconds after fluores cein injection. The contact lens was also used to examine
"T
the ciliary processes by fluorescein photogra phy through the colobomas of the iris and iris dialyses. Figure 4, taken 14 minutes after fluorescein injection, shows the ciliary process of a 24-year-old man with a large traumatic iridodialysis. SUMMARY
A Zeiss photo-slitlamp was modified for fluorescein angiography of the anterior seg ment. Changes included an air-cooled lighting section together with absorption filters and a concave lens. A Robot motor-camera w a s used and light was directed to the iris at a 60-degree angle. T h e anterior chamber angle and the ciliary processes were photographed through a one-mirror Goldmann contact lens. Smedegade
6 (4200) Slagelse,
Denmark
REFERENCES
1. Novotny, H. R. and Alvis, D. L. : A method of photographing fluorescence in circulating blood in the human retina. Circulation 24:82, 1961. 2. Jensen, V. A. and Lundbaek, K. : Fluorescence angiography of the iris in recent and long-term di abetes. Diabetologica 4:161, 1968.
WTO
Fig. 4 (Bruun-Jensen). The ciliary process of a 24-year-old man with a traumatic iridodialysis, about 14 minutes after fluorescein injection.