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Food preservation involves storing food in an ionized air environment ionized by corona discharge
Patent Report
The medium contains a sample solution to be analysed for containing metabolizing microorganisms. The fluroescent emission of the second dye is observed as to either: not changing to indicate the absence of metabolizing microorganisms in the sample; or changing to indicate the presence of metabolizing microorganisms in the sample. Preferably the first dye has an absorbance spectrum that changes either: (a) in response to physicochemical changes in the environment, especially changes in pH or in reduction-oxidation potential; or (b) when the dye is metabolized by specific microorgan059-94 isms. Food preservation involves storing food in an ionized air environment ionized by corona discharge Cut KK JP-05176732; 20 July 1993 Air is ionized with corona discharge and food is stored in the 060-94 ionized-air environment. Listeriamonocytogenesdetection by enzymatic nucleic acid amplification using oligonucleotide(s) derived from cu-haemolysin and/or /3-baemolysin virulence factors in polymerase chain reactions Candrian U CH-682156; 30 July 1993 Detection method for Listeria (L.) monocytogenes (mono.) comprises using the enzymatic nucleic acid amplification of hly (a-haemolysin) and/or iap (B-haemolysin) virulence factors. Preferably oligonucleotides (A) LO1 = S-CGGAGGTTCCGCAAAAGATG-3’; LO2 = 5’-CATCGACGGCA ACCTCGGA-3’; LO3 = 5’-CCATCTGTATAAGCTTTTGAAG-3’; and LO4= 5’-CCTCCAGAGTGATCGATGTT-3’ are used for hly and oligonucleotides (B) AD03= 5’-ACAAGCTGCACCTG’ITGCAG-3’; ADO7 = 5’-TGACAGCGTGTGTAGTAGTCA3’; ADO8 = 5’-GGCGCAGGTGTAGTTGCITG-3’ and ADO9 = 5’-CTACACAAGCAACTACACCT-3’ are used for iap. 061-94 Oligonucleotide(s) specific for Vihrio parahaemo/yticus, Escher&h&colior StaphyuococCus aureusused as primers or probes particularly for detecting food poisoning and in food inspection Schimadzu Corp. EP-556504; 25 August 1993
The following are claimed: (A) an oligonucleotide which is complementary to nucleic acid which comprises a target sequence encoding the thermostable direct haemolysinrelated haemolysin gene type 1 or type 2 (trhl trh2 gene) of Vibrio parahaemolyticus (VP), the oligonucleotide comprising a sequence selected from sequences (I)-(II) and corresponding complementary sequences: 5’-dGGCTCAAAAT GGTTAAGCG-3’ (I). \ , 5’-dCA’PITCCGCTCTCATATGC-3’
(II) Analysis of microorganisms etc. is based on distinctive electrorotation properties of complex formed between microparticle, linker and target Scientific Generics Ltd WO-9316383; 8 February
1993
Analytical method comprises forming a complex (C) between a microparticle (MP), linking residue (L) attached to MP, and a target (T). (C) has field rotation (especially electrorotation) properties which are identifiably different from those of MP plus L alone, and these different properties of (C) are observed. Also new are (1) kits comprising L-bearing MP plus (a) a sample receiving surface with a pattern of electrodes surrounding an analytical field for use in electrorotation assay and/or (b) an electrorotation label which can form a complex with Ti and (2) a microprorous filter for use
in this assay bearing a pattern of electrodes to define a sample receiving field and able to apply a rotating electrical field. Preferably L is an antibody (or fragment); antigen; nucleic acid (or analogue); probe or avidin(-like molecule). (C) 063-94 may also include a label bound to T. Detecting microorganism in drinks e.g. tangerine juice or oblong tea by adding binding microorganism in sample onto inner wall face of vessel, adding blockii liquid primary antibody etc. and enzyme labelled secondary antibody then washing etc. and detecting Wakayama Agri Bio Kenkyu Cent KK JP-05188056; 27 July 1993
Method comprises (a) adding a sample liquid of a drink in a test vessel and binding microorganism in the sample onto the inner wall face of the vessel while shaking the vessel, (b) adding a blocking liquid in the vessel and blocking part of the inner wall face not bound with the microorganism by the blocking liquid, (c) adding primary antibody to surface antigen of the microorganism, obtained by the immune injection of a microorganism presumed to be present in the drink in living state into an animal, in the vessel and reacting the primary antibody with the microorganism bound with the inner wall face, (d) adding an enzyme-labelled secondary antibody in the vessel and reaction the primary antibody with the secondary antibody, (e) washing away unreacted enzymelabelled secondary antibody by a washing water, (f) adding a substrate to the vessel and reacting the substrate with the enzyme-labelled secondary antibody, and (g) setting the test vessel at ELISA reader and detecting the microorganism at a wavelength according to the kind of the substrate. 064-94 Reducing aflatoxin content of seed, particularly peanuts by heating using microwave radiation while under vacuum in chamber Smucker J. M. Co. US-5230160; 27 July 1993
Method of reducing the aflatoxin content of contaminated seed having an initial moisture content of 6-17% comprises heating the seed in a chamber by microwave radiation to llO200°C for 15-45min and maintaining the chamber under a vacuum of 25-100Torr during the heating. The seed may be pretreated with water or ammonia water. Also claimed is a method comprising heating the seed to llO-200°C for about 28 min using microwave energy averaging a total of 20-23 kW and maintaining the chamber at vacuum during the heating. 065-94 Determining dry mass in fluids especially milk using freezing point and turbidity measurements Funke-Gerber
GmbH
DE-4206107; 2 September
1993
In a process to determine the dry mass of a fluid such as milk, the freezing point and turbidity are measured; the proportion of dissolved particles is derived from the freezing point measured, and the proportion of non-dissolved particles is derived from the turbidity, which together determine the dry mass calculated as present. The assembly comprises a freezing point determination unit and a turbidity measuring unit which are both linked to an electronic control and measurement device. 066-94 Process for monitoring stream of particles for continuous food processing inserting simulated particles with same physical characteristics as actual particles each with transponder into flow stream and measuring time of passage Kraft Gen. Foods Inc. EP-559401, 8 September
1993
The process comprises providing multiple simulated particles
Food Control 1994 Volume 5 Number 4
265
The medium contains a sample solution to be analysed for containing metabolizing microorganisms. The fluroescent emission of the second dye is observed as to either: not changing to indicate the absence of metabolizing microorganisms in the sample; or changing to indicate the presence of metabolizing microorganisms in the sample. Preferably the first dye has an absorbance spectrum that changes either: (a) in response to physicochemical changes in the environment, especially changes in pH or in reduction-oxidation potential; or (b) when the dye is metabolized by specific microorgan059-94 isms. Food preservation involves storing food in an ionized air environment ionized by corona discharge Cut KK JP-05176732; 20 July 1993 Air is ionized with corona discharge and food is stored in the 060-94 ionized-air environment. Listeriamonocytogenesdetection by enzymatic nucleic acid amplification using oligonucleotide(s) derived from cu-haemolysin and/or /3-baemolysin virulence factors in polymerase chain reactions Candrian U CH-682156; 30 July 1993 Detection method for Listeria (L.) monocytogenes (mono.) comprises using the enzymatic nucleic acid amplification of hly (a-haemolysin) and/or iap (B-haemolysin) virulence factors. Preferably oligonucleotides (A) LO1 = S-CGGAGGTTCCGCAAAAGATG-3’; LO2 = 5’-CATCGACGGCA ACCTCGGA-3’; LO3 = 5’-CCATCTGTATAAGCTTTTGAAG-3’; and LO4= 5’-CCTCCAGAGTGATCGATGTT-3’ are used for hly and oligonucleotides (B) AD03= 5’-ACAAGCTGCACCTG’ITGCAG-3’; ADO7 = 5’-TGACAGCGTGTGTAGTAGTCA3’; ADO8 = 5’-GGCGCAGGTGTAGTTGCITG-3’ and ADO9 = 5’-CTACACAAGCAACTACACCT-3’ are used for iap. 061-94 Oligonucleotide(s) specific for Vihrio parahaemo/yticus, Escher&h&colior StaphyuococCus aureusused as primers or probes particularly for detecting food poisoning and in food inspection Schimadzu Corp. EP-556504; 25 August 1993
The following are claimed: (A) an oligonucleotide which is complementary to nucleic acid which comprises a target sequence encoding the thermostable direct haemolysinrelated haemolysin gene type 1 or type 2 (trhl trh2 gene) of Vibrio parahaemolyticus (VP), the oligonucleotide comprising a sequence selected from sequences (I)-(II) and corresponding complementary sequences: 5’-dGGCTCAAAAT GGTTAAGCG-3’ (I). \ , 5’-dCA’PITCCGCTCTCATATGC-3’
(II) Analysis of microorganisms etc. is based on distinctive electrorotation properties of complex formed between microparticle, linker and target Scientific Generics Ltd WO-9316383; 8 February
1993
Analytical method comprises forming a complex (C) between a microparticle (MP), linking residue (L) attached to MP, and a target (T). (C) has field rotation (especially electrorotation) properties which are identifiably different from those of MP plus L alone, and these different properties of (C) are observed. Also new are (1) kits comprising L-bearing MP plus (a) a sample receiving surface with a pattern of electrodes surrounding an analytical field for use in electrorotation assay and/or (b) an electrorotation label which can form a complex with Ti and (2) a microprorous filter for use
in this assay bearing a pattern of electrodes to define a sample receiving field and able to apply a rotating electrical field. Preferably L is an antibody (or fragment); antigen; nucleic acid (or analogue); probe or avidin(-like molecule). (C) 063-94 may also include a label bound to T. Detecting microorganism in drinks e.g. tangerine juice or oblong tea by adding binding microorganism in sample onto inner wall face of vessel, adding blockii liquid primary antibody etc. and enzyme labelled secondary antibody then washing etc. and detecting Wakayama Agri Bio Kenkyu Cent KK JP-05188056; 27 July 1993
Method comprises (a) adding a sample liquid of a drink in a test vessel and binding microorganism in the sample onto the inner wall face of the vessel while shaking the vessel, (b) adding a blocking liquid in the vessel and blocking part of the inner wall face not bound with the microorganism by the blocking liquid, (c) adding primary antibody to surface antigen of the microorganism, obtained by the immune injection of a microorganism presumed to be present in the drink in living state into an animal, in the vessel and reacting the primary antibody with the microorganism bound with the inner wall face, (d) adding an enzyme-labelled secondary antibody in the vessel and reaction the primary antibody with the secondary antibody, (e) washing away unreacted enzymelabelled secondary antibody by a washing water, (f) adding a substrate to the vessel and reacting the substrate with the enzyme-labelled secondary antibody, and (g) setting the test vessel at ELISA reader and detecting the microorganism at a wavelength according to the kind of the substrate. 064-94 Reducing aflatoxin content of seed, particularly peanuts by heating using microwave radiation while under vacuum in chamber Smucker J. M. Co. US-5230160; 27 July 1993
Method of reducing the aflatoxin content of contaminated seed having an initial moisture content of 6-17% comprises heating the seed in a chamber by microwave radiation to llO200°C for 15-45min and maintaining the chamber under a vacuum of 25-100Torr during the heating. The seed may be pretreated with water or ammonia water. Also claimed is a method comprising heating the seed to llO-200°C for about 28 min using microwave energy averaging a total of 20-23 kW and maintaining the chamber at vacuum during the heating. 065-94 Determining dry mass in fluids especially milk using freezing point and turbidity measurements Funke-Gerber
GmbH
DE-4206107; 2 September
1993
In a process to determine the dry mass of a fluid such as milk, the freezing point and turbidity are measured; the proportion of dissolved particles is derived from the freezing point measured, and the proportion of non-dissolved particles is derived from the turbidity, which together determine the dry mass calculated as present. The assembly comprises a freezing point determination unit and a turbidity measuring unit which are both linked to an electronic control and measurement device. 066-94 Process for monitoring stream of particles for continuous food processing inserting simulated particles with same physical characteristics as actual particles each with transponder into flow stream and measuring time of passage Kraft Gen. Foods Inc. EP-559401, 8 September
1993
The process comprises providing multiple simulated particles
Food Control 1994 Volume 5 Number 4
265