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Four cytogenetic subgroups can be identified in lipomas
159
Abstracts
17
FOUR CYTOGENETIC SUBGROUPS CAN BE IDENTIFIED IN LIPOMAS. N. Mandahl, S. Helm, K. Arheden, A. Rydholm, H. Will6n, and F. Mitelman. Departments of Clinical Genetics, Orthopedics, and Clinical Pathology, Lurid University Hospital, S-221 85 Lund, Sweden.
We have investigated cytogenetically solitary and multiple lipomas from 60 patients after short-term culturing. Four cytogenetic subgroups could be distinguished among the 51 solitary lipomas, 36 of which had clonal chromosome aberrations. These subgroups were characterized by a) normal chromosome complement; b) hyperdiploid karyotypes including one or more supernumerary ring chromosomes; these tumors were all deep-seated atypical lipomas or had foci of atypia; c) diploid karyotypes with mostly balanced rearrangements involving 12q13-14, with t(3;12) as the only consistent aberration; d) hypodiploid or diploid karyotypes with other aberrations than ring chromosomes or rearrangement of 12q13-14. All 9 cases of multiple lipomas displayed normal chromosome complements.
18
Abstract not received.
19
CHROMOSOMAL REARRANGEMENTS IN SARCOMAS OF BONE AND SOFT-TISSUE. C. L6pezGin6s, R. Noguara, R. Gil, C. Carda, A. Pellin and A. Llombart-Bosch. Department of Pathology, University of Valencia. 46010 Valencia, Spain.
We present the cytogenetic analysis of 17 cases of sarcoma of bone and soft-tissue: 3 Ewing's sarcomas of bone (ES), 2 peripheral neuroepitheliomas (PN) 2 neuroblastomas (NB), 2 rhabdomyosarcomas (RB), 2 synovial sarcomas (SS), 1 leiomyosarcoma (LM), 1 myxoid liposarcoma (ML), 1 osteogenic sarcoma COS)and 3 undifferentiated sarcomas (US). The cells analyzed were derived from primary tumors (10/17 cases) and from hetarotrensplants into nude mice (9/17 cases). Cytogenetic examination was performed either by the direct method or short.term (2-8 days) culture. The study was performed followin8 trypsin-(; banding. Our results showed that 2 ES as well as the 2 PN presented the reciprocal translocation (11;22). One ES did not express this abnormality but it shared a deletion of chromosome 22. The 2 NB analyzed expressed typical DMS but no deletion of chromosome 1. The 2 RM showed alterations in chromosome 3 and one case expressed HSR. The 2 cases of SS presented translocation (X;18). The ML showed the t(12;16). The other cases analyzed, LM, aS and US presented S-15 identifiable markers and a variable number of abnormal chromosomes which could not be classified. Some of them showed DMS, HSR, PSC. With these results we can conclude that the socalled small round blue cell tumors are cytogenetically well typified and is possible to make a differential diagnosis within them. ES and PN expressed the same markers and are closely related entities separated from NB. Furthermore we can clearly distinguish ES and PN from NB, RB and SS. On the other hand, the other sarcomas analyzed (except ML) are poorly defined cytogenetically. They presented several abnormalities and further studies of several cases are required to typify them not only by karyotyping but also by molecular biology methods. Supported in part by the Social Security Cooperation (Exp. 88/0146)
Abstracts
17
FOUR CYTOGENETIC SUBGROUPS CAN BE IDENTIFIED IN LIPOMAS. N. Mandahl, S. Helm, K. Arheden, A. Rydholm, H. Will6n, and F. Mitelman. Departments of Clinical Genetics, Orthopedics, and Clinical Pathology, Lurid University Hospital, S-221 85 Lund, Sweden.
We have investigated cytogenetically solitary and multiple lipomas from 60 patients after short-term culturing. Four cytogenetic subgroups could be distinguished among the 51 solitary lipomas, 36 of which had clonal chromosome aberrations. These subgroups were characterized by a) normal chromosome complement; b) hyperdiploid karyotypes including one or more supernumerary ring chromosomes; these tumors were all deep-seated atypical lipomas or had foci of atypia; c) diploid karyotypes with mostly balanced rearrangements involving 12q13-14, with t(3;12) as the only consistent aberration; d) hypodiploid or diploid karyotypes with other aberrations than ring chromosomes or rearrangement of 12q13-14. All 9 cases of multiple lipomas displayed normal chromosome complements.
18
Abstract not received.
19
CHROMOSOMAL REARRANGEMENTS IN SARCOMAS OF BONE AND SOFT-TISSUE. C. L6pezGin6s, R. Noguara, R. Gil, C. Carda, A. Pellin and A. Llombart-Bosch. Department of Pathology, University of Valencia. 46010 Valencia, Spain.
We present the cytogenetic analysis of 17 cases of sarcoma of bone and soft-tissue: 3 Ewing's sarcomas of bone (ES), 2 peripheral neuroepitheliomas (PN) 2 neuroblastomas (NB), 2 rhabdomyosarcomas (RB), 2 synovial sarcomas (SS), 1 leiomyosarcoma (LM), 1 myxoid liposarcoma (ML), 1 osteogenic sarcoma COS)and 3 undifferentiated sarcomas (US). The cells analyzed were derived from primary tumors (10/17 cases) and from hetarotrensplants into nude mice (9/17 cases). Cytogenetic examination was performed either by the direct method or short.term (2-8 days) culture. The study was performed followin8 trypsin-(; banding. Our results showed that 2 ES as well as the 2 PN presented the reciprocal translocation (11;22). One ES did not express this abnormality but it shared a deletion of chromosome 22. The 2 NB analyzed expressed typical DMS but no deletion of chromosome 1. The 2 RM showed alterations in chromosome 3 and one case expressed HSR. The 2 cases of SS presented translocation (X;18). The ML showed the t(12;16). The other cases analyzed, LM, aS and US presented S-15 identifiable markers and a variable number of abnormal chromosomes which could not be classified. Some of them showed DMS, HSR, PSC. With these results we can conclude that the socalled small round blue cell tumors are cytogenetically well typified and is possible to make a differential diagnosis within them. ES and PN expressed the same markers and are closely related entities separated from NB. Furthermore we can clearly distinguish ES and PN from NB, RB and SS. On the other hand, the other sarcomas analyzed (except ML) are poorly defined cytogenetically. They presented several abnormalities and further studies of several cases are required to typify them not only by karyotyping but also by molecular biology methods. Supported in part by the Social Security Cooperation (Exp. 88/0146)