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Fresh allogenous Achilles tendon grafting in rabbit model
S270
Abstracts / Bone 48 (2011) S266–S271
Image/Graph
This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.667
PP537-T Risk of fracture in patients with myasthenia gravis: The general practice research database S. Pouwels a, ⁎, M. Javaid b, A. de Boer a, F. de Vries a a Pharmacoepidemiology & Pharmacotherapy, University of Utrecht, Utrecht, Netherlands b University of Oxford, Institute of Musculoskeletal Sciences, Oxford, UK
Asian women had significantly higher total density and trabecular density at 4% tibia than C women; but they had significantly lower cortical density at 14% and 38% tibia sites. Asian women had also significant lower total area and trabecular area (p < 0.05) at 4% tibia site. There were no significant differences in vBMD between C and A in radius sites. (Not shown in the table) however, at the 4% radius, Asians have significant lower bone mass (p < 0.05), total area (p < 0.01) and trabecular area (p < 0.01) than Caucasians. Our finding supports the results of Ward et al. (2007) where there was no difference in vBMD at the radius between A and C women. Our novel findings for differences in the tibia sites for A and C women require further investigations as there are no data examining this bone site in ethnic groups. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
References [1] Roy, et al. Osteoporosis Int 2007;41:117–21. [2] Ward, et al. Bone 2005;16:1254–62. doi:10.1016/j.bone.2011.03.666
PP536-M Acute bone response to whole body vibration in pre-pubertal boys R.C. Harrison a,b, ⁎, K. Ward c, C. Horne d, N.J. Bishop a,b a Sheffield Children's Hospital, UK b University of Sheffield, Sheffield, UK c Nutrition and Bone Health, MRC Human Nutrition Research, Cambridge, UK d Sheffield Hallam University, Sheffield, UK Abstract: Introduction and Aim of study: Improved biomechanical outcomes and increased bone size, density and formation as a result of whole body vibration (WBV) have been shown in animal models. Observations over a period of months in postmenopausal women, young women with low bone mass and children with disabling conditions, have shown that up to 10 minutes a day of WBV can significantly increase bone mass. Data regarding the effects of WBV in paediatric populations is limited; additionally the immediate response of bone to WBV has not been investigated. The aim of this study is to determine an acute bone response to WBV healthy pre-pubertal boys. Methods: A two phase study was conducted. In phase one (completed) 12 healthy boys aged 9–12 years were recruited to stand on either the Juvent 1000 or Galileo Advanced vibrating platforms once only for 10 minutes. Pre-, 10 and 60 minutes post vibration a blood sample was taken to measure changes in the bone formation marker P1NP and resorption marker CTx. Pre- and post vibration a thermal image was taken of the boys' legs to measure skin surface temperature as a surrogate for blood flow and muscle activity. All boys were asked about the amount of exercise and sport they had participated in over the previous seven days. A two sample t-test was used to analyse the baseline characteristics and repeated measures ANOVA for change in P1NP and CTx. Results: There was no significant difference in mean height, weight or amount of exercise between the two intervention groups. Age adjusted body mass index of the boys in the Galileo group was significantly greater than those in the Juvent group. The mean change in skin surface temperature from baseline was 2.4 °C greater in boys exposed to WBV on the Galileo than by the Juvent platform (p = < 0.01). When assessed visually a different pattern of temperature distribution was apparent between the pre- and post Galileo WBV thermal image. WBV did not have any significant effect on P1NP or CTx within or between the vibration platform groups. Conclusion: Despite influencing skin surface temperature, possibly indicating increased muscle activity in the Galileo group, one episode of WBV in pre-pubertal boys had no effect on bone turnover markers using either platform. A further 24 boys are being recruited to stand on one of the two vibrating platforms for 10 minutes on either 3 or 5 consecutive days.
Abstract: Introduction: Myasthenia Gravis (MG) is a neuromuscular disease with symptoms of muscle weakness and fatiguability. MG has been associated with falling and glucocorticoid induced osteoporosis. Therefore, the aim of our study is to evaluate the risk of fracture after onset of myasthenia gravis. Methods: We conducted a retrospective cohort study (1987–2009, n = 7,458) using the UK General Practice Research Database. Each MG patient was matched by age, sex, calendar time, and practice to up to 6 patients without a history of MG. The overall risk of fracture after onset of MG and the risk stratified by use of oral glucocorticoids and by drugs affecting the central nervous system (CNS) was determined. Results: No increased fracture risk was observed in patients with incident MG (adjusted hazard ratio [AHR] 1.11; 95% confidence interval [CI], 0.84–1.47). Use of oral glucocorticoids did not significantly increase fracture risk, but MG patients using antidepressants or anxiolytics/sedatives in the previous 6 months were at a significantly 2-fold increased fracture risk (AHR 1.99; 95% CI, 1.07–3.69 and 2.15; 95% CI, 1.14–4.03 respectively). The highest risk was observed among MG patients who had been exposed to anticonvulsants in the previous 6 months (AHR 4.98; 95% CI, 2.68–9.26). Conclusion: MG seems not to be associated with an increased fracture risk, except for patients using CNS medication. Moreover, use of (very high dosages of) oral glucocorticoids among MG patients did not alter fracture risk, for which the underlying mechanism remains unclear. Fracture risk assessment may be indicated among patients with MG who have recently used CNS medication. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: S. Pouwels Grant / Research Support from ECTS PhD Studentship Award 2009, M. Javaid: None Declared, A. de Boer: None Declared, F. de Vries: None Declared.
doi:10.1016/j.bone.2011.03.668
PP538-S Fresh allogenous Achilles tendon grafting in rabbit model S.E. Shariati a, ⁎, Z. Shafieisarvestani b, A. Bighamsadegh a a Department of Veterinary Surgery and Radiology, Shahrekord University, Shahrekord, Islamic Republic of Iran b Department of Veterinary Surgery and Radiology, Shiraz University, Shiraz, Islamic Republic of Iran
Abstract: Tendons are soft connective tissues consisting of parallel collagen fibers embedded within an extracellular matrix. Blood supply to the tendon is reported to be poor; thereby healing often was progressed slowly. Autogenic, allogenic and xenogenic tendon transplantations have been performed in reconstructive tendon surgery. There is little information on fresh allogenic tendon transplantation experimentally. The aim of this study is to evaluate fresh autogenous and allogenous tendon graft in the rabbit to determine which one is better, clinically and histopathologically. Ten male New Zealand Albino rabbits 1 year old and weighing 3.5 ± 0.5 kg were used in this study. About 3 cm of the superficial flexor tendon was resected and a created defect was filled in five rabbits (group I) with same 3 cm harvested tendon. Then transplanted tendon was sutured with 2/0 polypropylene in a single Modified Kessler suture pattern (Fig 1) and in group II (other five rabbits), the harvested segment changed between rabbits and created defects were filled with changed segment allogenous tendon. The tendon sheet was sutured over the transplanted tendon completely in the all groups. The main histopathological and gross evaluation showed graft acceptance by recipients. Our results showed that fresh allogenic tendon was transplanted in rabbit model and significant tissue reaction and graft rejection were not observed. This article is part of a Special Issue entitled ECTS 2011. Keywords: Fresh allogenic; Fresh autogenic; Tendon transplantation; Rabbit model. Disclosure of interest: S. E. Shariati Grant / Research Support from There is no conflict of interest, Z. Shafieisarvestani Grant / Research Support from There is no conflict of interest, A. Bighamsadegh: None Declared. doi:10.1016/j.bone.2011.03.669
Abstracts / Bone 48 (2011) S266–S271
Image/Graph
This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
doi:10.1016/j.bone.2011.03.667
PP537-T Risk of fracture in patients with myasthenia gravis: The general practice research database S. Pouwels a, ⁎, M. Javaid b, A. de Boer a, F. de Vries a a Pharmacoepidemiology & Pharmacotherapy, University of Utrecht, Utrecht, Netherlands b University of Oxford, Institute of Musculoskeletal Sciences, Oxford, UK
Asian women had significantly higher total density and trabecular density at 4% tibia than C women; but they had significantly lower cortical density at 14% and 38% tibia sites. Asian women had also significant lower total area and trabecular area (p < 0.05) at 4% tibia site. There were no significant differences in vBMD between C and A in radius sites. (Not shown in the table) however, at the 4% radius, Asians have significant lower bone mass (p < 0.05), total area (p < 0.01) and trabecular area (p < 0.01) than Caucasians. Our finding supports the results of Ward et al. (2007) where there was no difference in vBMD at the radius between A and C women. Our novel findings for differences in the tibia sites for A and C women require further investigations as there are no data examining this bone site in ethnic groups. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
References [1] Roy, et al. Osteoporosis Int 2007;41:117–21. [2] Ward, et al. Bone 2005;16:1254–62. doi:10.1016/j.bone.2011.03.666
PP536-M Acute bone response to whole body vibration in pre-pubertal boys R.C. Harrison a,b, ⁎, K. Ward c, C. Horne d, N.J. Bishop a,b a Sheffield Children's Hospital, UK b University of Sheffield, Sheffield, UK c Nutrition and Bone Health, MRC Human Nutrition Research, Cambridge, UK d Sheffield Hallam University, Sheffield, UK Abstract: Introduction and Aim of study: Improved biomechanical outcomes and increased bone size, density and formation as a result of whole body vibration (WBV) have been shown in animal models. Observations over a period of months in postmenopausal women, young women with low bone mass and children with disabling conditions, have shown that up to 10 minutes a day of WBV can significantly increase bone mass. Data regarding the effects of WBV in paediatric populations is limited; additionally the immediate response of bone to WBV has not been investigated. The aim of this study is to determine an acute bone response to WBV healthy pre-pubertal boys. Methods: A two phase study was conducted. In phase one (completed) 12 healthy boys aged 9–12 years were recruited to stand on either the Juvent 1000 or Galileo Advanced vibrating platforms once only for 10 minutes. Pre-, 10 and 60 minutes post vibration a blood sample was taken to measure changes in the bone formation marker P1NP and resorption marker CTx. Pre- and post vibration a thermal image was taken of the boys' legs to measure skin surface temperature as a surrogate for blood flow and muscle activity. All boys were asked about the amount of exercise and sport they had participated in over the previous seven days. A two sample t-test was used to analyse the baseline characteristics and repeated measures ANOVA for change in P1NP and CTx. Results: There was no significant difference in mean height, weight or amount of exercise between the two intervention groups. Age adjusted body mass index of the boys in the Galileo group was significantly greater than those in the Juvent group. The mean change in skin surface temperature from baseline was 2.4 °C greater in boys exposed to WBV on the Galileo than by the Juvent platform (p = < 0.01). When assessed visually a different pattern of temperature distribution was apparent between the pre- and post Galileo WBV thermal image. WBV did not have any significant effect on P1NP or CTx within or between the vibration platform groups. Conclusion: Despite influencing skin surface temperature, possibly indicating increased muscle activity in the Galileo group, one episode of WBV in pre-pubertal boys had no effect on bone turnover markers using either platform. A further 24 boys are being recruited to stand on one of the two vibrating platforms for 10 minutes on either 3 or 5 consecutive days.
Abstract: Introduction: Myasthenia Gravis (MG) is a neuromuscular disease with symptoms of muscle weakness and fatiguability. MG has been associated with falling and glucocorticoid induced osteoporosis. Therefore, the aim of our study is to evaluate the risk of fracture after onset of myasthenia gravis. Methods: We conducted a retrospective cohort study (1987–2009, n = 7,458) using the UK General Practice Research Database. Each MG patient was matched by age, sex, calendar time, and practice to up to 6 patients without a history of MG. The overall risk of fracture after onset of MG and the risk stratified by use of oral glucocorticoids and by drugs affecting the central nervous system (CNS) was determined. Results: No increased fracture risk was observed in patients with incident MG (adjusted hazard ratio [AHR] 1.11; 95% confidence interval [CI], 0.84–1.47). Use of oral glucocorticoids did not significantly increase fracture risk, but MG patients using antidepressants or anxiolytics/sedatives in the previous 6 months were at a significantly 2-fold increased fracture risk (AHR 1.99; 95% CI, 1.07–3.69 and 2.15; 95% CI, 1.14–4.03 respectively). The highest risk was observed among MG patients who had been exposed to anticonvulsants in the previous 6 months (AHR 4.98; 95% CI, 2.68–9.26). Conclusion: MG seems not to be associated with an increased fracture risk, except for patients using CNS medication. Moreover, use of (very high dosages of) oral glucocorticoids among MG patients did not alter fracture risk, for which the underlying mechanism remains unclear. Fracture risk assessment may be indicated among patients with MG who have recently used CNS medication. This article is part of a Special Issue entitled ECTS 2011. Disclosure of interest: S. Pouwels Grant / Research Support from ECTS PhD Studentship Award 2009, M. Javaid: None Declared, A. de Boer: None Declared, F. de Vries: None Declared.
doi:10.1016/j.bone.2011.03.668
PP538-S Fresh allogenous Achilles tendon grafting in rabbit model S.E. Shariati a, ⁎, Z. Shafieisarvestani b, A. Bighamsadegh a a Department of Veterinary Surgery and Radiology, Shahrekord University, Shahrekord, Islamic Republic of Iran b Department of Veterinary Surgery and Radiology, Shiraz University, Shiraz, Islamic Republic of Iran
Abstract: Tendons are soft connective tissues consisting of parallel collagen fibers embedded within an extracellular matrix. Blood supply to the tendon is reported to be poor; thereby healing often was progressed slowly. Autogenic, allogenic and xenogenic tendon transplantations have been performed in reconstructive tendon surgery. There is little information on fresh allogenic tendon transplantation experimentally. The aim of this study is to evaluate fresh autogenous and allogenous tendon graft in the rabbit to determine which one is better, clinically and histopathologically. Ten male New Zealand Albino rabbits 1 year old and weighing 3.5 ± 0.5 kg were used in this study. About 3 cm of the superficial flexor tendon was resected and a created defect was filled in five rabbits (group I) with same 3 cm harvested tendon. Then transplanted tendon was sutured with 2/0 polypropylene in a single Modified Kessler suture pattern (Fig 1) and in group II (other five rabbits), the harvested segment changed between rabbits and created defects were filled with changed segment allogenous tendon. The tendon sheet was sutured over the transplanted tendon completely in the all groups. The main histopathological and gross evaluation showed graft acceptance by recipients. Our results showed that fresh allogenic tendon was transplanted in rabbit model and significant tissue reaction and graft rejection were not observed. This article is part of a Special Issue entitled ECTS 2011. Keywords: Fresh allogenic; Fresh autogenic; Tendon transplantation; Rabbit model. Disclosure of interest: S. E. Shariati Grant / Research Support from There is no conflict of interest, Z. Shafieisarvestani Grant / Research Support from There is no conflict of interest, A. Bighamsadegh: None Declared. doi:10.1016/j.bone.2011.03.669