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Fructus Ligustri Lucidi enhances osteogenesis and represses adipogenesis of rat mesenchymal stem cells
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ABSTRACTS / Bone 43 (2008) S94–S110
Results: 23 post-menopause women had an average age of 51.67 ± 1.29 years, 8 of them had abnormal BMD (abnormal rate 27.6%).There were no differences between the two groups (t = 0.873, P = 0.387), but BMD abnormal rate in post-menopause group was obviously higher than that in nulli-menopause group (x2 = 4.461, P = 0.035, r = 0.293). Conclusions: There is a significant relevance between abnormal lumbar dorsaventral bone mineral density and menopausal in women in Nanjing City. The beforehand prevention of the postmenopausal osteoporosis should be done before menopause. doi:10.1016/j.bone.2008.07.225
adipogenic induction medium. The osteogenic and adipogenic markers were assessed by biochemical assays and real-time PCR. Results: During osteogenic differentiation, FLL water extract significantly increased alkaline phosphatase (ALP) activity and matrix mineralization of rMSCs in dose-dependent manner in the range of concentrations without cytotoxicity (b100 μg/mL) for 7 days and 14 days. Conclusions: Our results indicate that FLL water extract can enhance the osteogenesis and inhibit the adipogenesis of rMSCs, suggesting that it might be useful as an alternative therapeutic agent for preventing osteoporosis. doi:10.1016/j.bone.2008.07.227
F42 Effects of a vitamin D2 supplement on physical function in postmenopausal women: A prospective randomized controlled study Noriaki Yamamoto 1, Hideaki E. Takahashi 2 1 Niigata Rehabilitation Hospital, Niigata, Japan 2 Niigata University of Health and Welfare, Niigata, Japan Background: Fracture prevention in the elderly is an important issue in the aging society. A recent systematic review of vitamin D supplementation concluded that it reduced the risk of fall. We conducted a randomized controlled trial to evaluate the effect of vitamin D2 powder from Maitake mushroom with calcium on bone mineral density and motor function compared with calcium alone. Methods: We randomly divided 46 postmenopausal women (aged 68.7 ± 9.9) into two groups with age, A; calcium alone group (300 mg milk calcium), B; calcium + vitamin D2 combination group (300 mg milk calcium + 750 IU ergocalciferol). Participants in both groups received these supplements for 6 months. Main outcome measures were bone mineral density (BMD) and motor function tests. Results: Serum 25(OH)D concentration is 20.52 ± 4.53 (ng/ml) in the alone group, 21.52 ± 4.12 (ng/ml) in the combination group at the beginning. After 6 months of administration of supplement, slightly increasing of 25(OH)D level were observed in the combination group. No significant changes were observed in BMD in femoral neck and lumbar spine, but the motor function test showed significant improvement in 10 m walking speed 6%(p b 0.01) and one leg standing time 30%(p b 0.01) in combination group after six months treatment. Conclusion: Vitamin D2 ergocalciferol supplementation for 6 months improves motor function in postmenopausal women. It may induce the protective effect against falling in elderly. doi:10.1016/j.bone.2008.07.226
F43 Fructus Ligustri Lucidi enhances osteogenesis and represses adipogenesis of rat mesenchymal stem cells Chun Hay Ko, Wing Sum Siu, Yuk Ha Lee, Kwok Pui Fung, Ping Chung Leung Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China Objectives: The aim of our study is to investigate the role of Fructus Ligustri Lucidi (FLL) water extract in rat mesenchymal stem cells (rMSCs), undergoing osteogenic or adipogenic induction. Methods: rMSCs were collected from the femoral diaphysis of 300 g SD rats and then cultured with FLL water extract in either osteogenic or
F44 The effect of simvastatin on bone tissue BMP-2 expression in diabetic rats Juan Wang, Juan Chen Second Affiliated Hospital of Chongqing Medical University, Chongqing, China Objective: To investigate the effect of interventional therapy of simvastatin on changes of bone tissue BMP-2, bone mineral density and bone metabolism in diabetic rats. Methods: Thirty male SD rats, aged 4 months, were randomly divided into three groups of 10 rats: normal control group (A), diabetic group (B), simvastatin group (C). After the rats of group B and C had established the diabetic model by intraperitoneal injection of streptozotocin, the rats of group C were administered orally simvastatin (5 mg/kg·d), Groups A and B were administered orally 1.5 ml normal saline every day. All group rats were killed at the end of 14 weeks, then bone BMP2-mRNA, bone mineral density, and bone metabolism were investigated. Results: The level of BMP2-mRNA rose in group C compared to group B (P b 0.01). Conclusion: Simvastatin could enhance the bone tissue BMP2mRNA expression and increase the BMD, and improve the bone metabolism in diabetic rats. This can provide experimental basis on treating diabetic osteoporosis. doi:10.1016/j.bone.2008.07.228
F45 Gorham's disease —– One case with extensive massive osteolysis Xiaodong He, Yan Jiang, Weibo Xia, Feng Gu, Ou Wang, Mei Li, Xunwu Meng, Xiaoping Xing, Wei Yu Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China Background: Gorham's disease (massive osteolysis), initially described by Gorham and colleagues in 1954, is a rare skeletal disorder of which the aetiology and pathogenesis remain unknown. There are about 200 cases have been reported in the literature to date. Gorham's disease may throughout the body, commonly involved sites including the mandible (15%), ribs (12%), scapula (10%), humerus (8%), pelvis (10%), and femur (11%). We reported a case of Gorham syndrome affected extensively. Patient and manifestations: A female of 36 years old. Before she visited us, she had a 29-year history of scoliosis and 25-year history of lower back pain. At 36 years of age, aggressive pain on her bilateral hip and gradual limitation of activity occurred. Plain radiography and
ABSTRACTS / Bone 43 (2008) S94–S110
Results: 23 post-menopause women had an average age of 51.67 ± 1.29 years, 8 of them had abnormal BMD (abnormal rate 27.6%).There were no differences between the two groups (t = 0.873, P = 0.387), but BMD abnormal rate in post-menopause group was obviously higher than that in nulli-menopause group (x2 = 4.461, P = 0.035, r = 0.293). Conclusions: There is a significant relevance between abnormal lumbar dorsaventral bone mineral density and menopausal in women in Nanjing City. The beforehand prevention of the postmenopausal osteoporosis should be done before menopause. doi:10.1016/j.bone.2008.07.225
adipogenic induction medium. The osteogenic and adipogenic markers were assessed by biochemical assays and real-time PCR. Results: During osteogenic differentiation, FLL water extract significantly increased alkaline phosphatase (ALP) activity and matrix mineralization of rMSCs in dose-dependent manner in the range of concentrations without cytotoxicity (b100 μg/mL) for 7 days and 14 days. Conclusions: Our results indicate that FLL water extract can enhance the osteogenesis and inhibit the adipogenesis of rMSCs, suggesting that it might be useful as an alternative therapeutic agent for preventing osteoporosis. doi:10.1016/j.bone.2008.07.227
F42 Effects of a vitamin D2 supplement on physical function in postmenopausal women: A prospective randomized controlled study Noriaki Yamamoto 1, Hideaki E. Takahashi 2 1 Niigata Rehabilitation Hospital, Niigata, Japan 2 Niigata University of Health and Welfare, Niigata, Japan Background: Fracture prevention in the elderly is an important issue in the aging society. A recent systematic review of vitamin D supplementation concluded that it reduced the risk of fall. We conducted a randomized controlled trial to evaluate the effect of vitamin D2 powder from Maitake mushroom with calcium on bone mineral density and motor function compared with calcium alone. Methods: We randomly divided 46 postmenopausal women (aged 68.7 ± 9.9) into two groups with age, A; calcium alone group (300 mg milk calcium), B; calcium + vitamin D2 combination group (300 mg milk calcium + 750 IU ergocalciferol). Participants in both groups received these supplements for 6 months. Main outcome measures were bone mineral density (BMD) and motor function tests. Results: Serum 25(OH)D concentration is 20.52 ± 4.53 (ng/ml) in the alone group, 21.52 ± 4.12 (ng/ml) in the combination group at the beginning. After 6 months of administration of supplement, slightly increasing of 25(OH)D level were observed in the combination group. No significant changes were observed in BMD in femoral neck and lumbar spine, but the motor function test showed significant improvement in 10 m walking speed 6%(p b 0.01) and one leg standing time 30%(p b 0.01) in combination group after six months treatment. Conclusion: Vitamin D2 ergocalciferol supplementation for 6 months improves motor function in postmenopausal women. It may induce the protective effect against falling in elderly. doi:10.1016/j.bone.2008.07.226
F43 Fructus Ligustri Lucidi enhances osteogenesis and represses adipogenesis of rat mesenchymal stem cells Chun Hay Ko, Wing Sum Siu, Yuk Ha Lee, Kwok Pui Fung, Ping Chung Leung Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China Objectives: The aim of our study is to investigate the role of Fructus Ligustri Lucidi (FLL) water extract in rat mesenchymal stem cells (rMSCs), undergoing osteogenic or adipogenic induction. Methods: rMSCs were collected from the femoral diaphysis of 300 g SD rats and then cultured with FLL water extract in either osteogenic or
F44 The effect of simvastatin on bone tissue BMP-2 expression in diabetic rats Juan Wang, Juan Chen Second Affiliated Hospital of Chongqing Medical University, Chongqing, China Objective: To investigate the effect of interventional therapy of simvastatin on changes of bone tissue BMP-2, bone mineral density and bone metabolism in diabetic rats. Methods: Thirty male SD rats, aged 4 months, were randomly divided into three groups of 10 rats: normal control group (A), diabetic group (B), simvastatin group (C). After the rats of group B and C had established the diabetic model by intraperitoneal injection of streptozotocin, the rats of group C were administered orally simvastatin (5 mg/kg·d), Groups A and B were administered orally 1.5 ml normal saline every day. All group rats were killed at the end of 14 weeks, then bone BMP2-mRNA, bone mineral density, and bone metabolism were investigated. Results: The level of BMP2-mRNA rose in group C compared to group B (P b 0.01). Conclusion: Simvastatin could enhance the bone tissue BMP2mRNA expression and increase the BMD, and improve the bone metabolism in diabetic rats. This can provide experimental basis on treating diabetic osteoporosis. doi:10.1016/j.bone.2008.07.228
F45 Gorham's disease —– One case with extensive massive osteolysis Xiaodong He, Yan Jiang, Weibo Xia, Feng Gu, Ou Wang, Mei Li, Xunwu Meng, Xiaoping Xing, Wei Yu Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China Background: Gorham's disease (massive osteolysis), initially described by Gorham and colleagues in 1954, is a rare skeletal disorder of which the aetiology and pathogenesis remain unknown. There are about 200 cases have been reported in the literature to date. Gorham's disease may throughout the body, commonly involved sites including the mandible (15%), ribs (12%), scapula (10%), humerus (8%), pelvis (10%), and femur (11%). We reported a case of Gorham syndrome affected extensively. Patient and manifestations: A female of 36 years old. Before she visited us, she had a 29-year history of scoliosis and 25-year history of lower back pain. At 36 years of age, aggressive pain on her bilateral hip and gradual limitation of activity occurred. Plain radiography and