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Fruit juices as a new source of probiotics in a lactose free diet
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Abstracts / New Biotechnology 33S (2016) S1–S213
attractive miRNAs were highly upregulated upon the TNF-alpha stimulation. Our results strongly suggest that synovial fibroblasts alter the exosomal miRNA profiles in response to inflammatory stimulus, which may involve in the pathogenesis of RA [2]. References [1] Shibasaki S, et al. FEBS Open Bio; 2016 [in press]. [2] Takamura Y, et al. submitted.
http://dx.doi.org/10.1016/j.nbt.2016.06.972
P3-4 Process development of a high-purity hyaluronidase from sheep testis
and
virally-safe
In Seop Kim 1,∗ , Jung Eun Bae 2 , Sang Eun Han 2 , Young Jun Baek 1 , Hana Kim 3 1
Hannam University, Republic of Korea BioPS Co. Ltd., Republic of Korea 3 BMI Korea, Republic of Korea 2
The most popular sources of probiotics are yoghurt, milk and kefir (a milky fermented beverage). However, the deficiency in an enzyme lactase that catalyzes hydrolysis of milk disaccharide, lactose, into glucose and galactose, appears as a serious problem of many populations worldwide and makes the consumption of probiotic-enriched milky products impossible. In the study we tested fresh and fermented juices of honeyberry (Lonicera caerulea var. kamtschatica cv. ‘Duet’) as well as red (Rubus idaeus cvs. ‘Polana’ and ‘Laszka’/‘Lashka’) and black (R. occidentalis cv. ‘Litacz’) raspberries, as a habitat of 5 probiotic strains: Lactobacillus acidophilus (LA-5), L. casei, L. kefiri, L. plantarum, and L. rhamnosus. Black raspberry ‘Litacz’ was shown to be strongly inhibitory for probiotic lactobacilli, presumably for its high content of phenolics with antibacterial activity. Similarly, all the fermented juices caused dramatic loss in bacterial frequency after 7 days of incubation at 4 ◦ C. These results were contrasted by fresh honeyberry and red raspberry juices which proved to maintain survivability of probiotics above 50% of the original inoculum density (39 –109 CFU ml−1 ) under the same conditions. In the next stage of our research the health-beneficial properties of fresh juices enriched with probiotic bacteria will be investigated. http://dx.doi.org/10.1016/j.nbt.2016.06.974
The hyaluronidases (EC 3.2.1.35) are a family of enzymes that degrade hyaluronic acid. By catalyzing the hydrolysis of hyaluronan, a constituent of the extracellular matrix, hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and delivery. We developed a industrialscale manufacturing process of a high-purity and virus-safe hyaluronidase from ovine testis. The purification process contains blue-sepharose affinity chromatography, mono S cation-exchange chromatography, heparin sepharose affinity chromatography, and virus filtration process. In order to evaluate the efficacy of blue sepharose chromatography, heparin sepharose chromatography, and virus filtration process for the removal of possible contaminating viruses during the manufacture of hyaluronidase, a variety of experimental model viruses including ovine visna virus, bovine herpesvirus, bovine parainfluenza virus 3, and bovine parvovirus were chosen. All the viruses tested were completely removed during virus filtration process. Also all the viruses were effectively separated from hyaluronidase during blue sepharose chromatography and heparin sepharose chromatography. These results indicate that the manufacturing process for hyaluronidase from sheep testis has sufficient virus-reducing capacity to achieve a high margin of virus safety. http://dx.doi.org/10.1016/j.nbt.2016.06.973
P3-5 Fruit juices as a new source of probiotics in a lactose free diet Anna Kostecka-Gugała 1,∗ , Aleksandra Mech-Nowak 2 , Aleksandra Duda-Chodak 3 , Paweł Kaszycki 1
P3-6 Phosphorothioate analogues of lysophosphatidylcholine as signaling molecules for type 2 diabetes-related membrane receptors Maria Koziołkiewicz ∗ , Anna Drzazga, Edyta Gendaszewska-Darmach Lodz University of Technology, Poland During last two decades it has been evidenced that lysophospholipids (LPLs) are important signaling molecules involved in many physiological and pathophysiological processes. They contain a glycerol backbone with one fatty acid residue ester-linked at the sn-1 or sn-2 position and a phosphate residue at the sn-3 position as a part of hydrophilic “head”. To the best characterized LPLs belong sphingosine-1-phosphate, lysophosphatidic acids (LPAs) and cyclic phosphatidic acids (cPAs) structurally related to LPAs. However, recent studies indicate that also lysophosphatidylcholine (LPC) is an important signaling molecule capable of modulation of insulin secretion. Our studies have been focused on phosphorothioate LPC analogues containing sulfur atoms and various acyl residues and their ability to activate two main GPCR receptors related to insulin secretion: GPR119 and GPR55. Structural differences between native LPCs and their synthetic analogues have a great impact on both the strength and manner of the influence on in vitro  cell models, including activation of different signaling pathways (cAMP synthesis and Ca2+ release) and, what is the most important, glucose-stimulated insulin secretion.
1
Institute of Plant Biology and Biotechnology, Poland Faculty of Biotechnology and Horticulture, Poland 3 University of Agriculture in Krakow, Poland 2
Probiotic bacteria are responsible for a variety of health benefits such as maintenance of individual intestinal microbiota during antibiotic treatment, support of the immune system, decreased threat of colonization with potentially pathogenic gastrointestinal microorganisms, protection of proteins and lipids against oxidative damage, and even help to relieve depression or prevent obesity.
This project is supported by the Polish National Science Centre (NCN, grant no. 2013/11/N/NZ5/00270). http://dx.doi.org/10.1016/j.nbt.2016.06.975
Abstracts / New Biotechnology 33S (2016) S1–S213
attractive miRNAs were highly upregulated upon the TNF-alpha stimulation. Our results strongly suggest that synovial fibroblasts alter the exosomal miRNA profiles in response to inflammatory stimulus, which may involve in the pathogenesis of RA [2]. References [1] Shibasaki S, et al. FEBS Open Bio; 2016 [in press]. [2] Takamura Y, et al. submitted.
http://dx.doi.org/10.1016/j.nbt.2016.06.972
P3-4 Process development of a high-purity hyaluronidase from sheep testis
and
virally-safe
In Seop Kim 1,∗ , Jung Eun Bae 2 , Sang Eun Han 2 , Young Jun Baek 1 , Hana Kim 3 1
Hannam University, Republic of Korea BioPS Co. Ltd., Republic of Korea 3 BMI Korea, Republic of Korea 2
The most popular sources of probiotics are yoghurt, milk and kefir (a milky fermented beverage). However, the deficiency in an enzyme lactase that catalyzes hydrolysis of milk disaccharide, lactose, into glucose and galactose, appears as a serious problem of many populations worldwide and makes the consumption of probiotic-enriched milky products impossible. In the study we tested fresh and fermented juices of honeyberry (Lonicera caerulea var. kamtschatica cv. ‘Duet’) as well as red (Rubus idaeus cvs. ‘Polana’ and ‘Laszka’/‘Lashka’) and black (R. occidentalis cv. ‘Litacz’) raspberries, as a habitat of 5 probiotic strains: Lactobacillus acidophilus (LA-5), L. casei, L. kefiri, L. plantarum, and L. rhamnosus. Black raspberry ‘Litacz’ was shown to be strongly inhibitory for probiotic lactobacilli, presumably for its high content of phenolics with antibacterial activity. Similarly, all the fermented juices caused dramatic loss in bacterial frequency after 7 days of incubation at 4 ◦ C. These results were contrasted by fresh honeyberry and red raspberry juices which proved to maintain survivability of probiotics above 50% of the original inoculum density (39 –109 CFU ml−1 ) under the same conditions. In the next stage of our research the health-beneficial properties of fresh juices enriched with probiotic bacteria will be investigated. http://dx.doi.org/10.1016/j.nbt.2016.06.974
The hyaluronidases (EC 3.2.1.35) are a family of enzymes that degrade hyaluronic acid. By catalyzing the hydrolysis of hyaluronan, a constituent of the extracellular matrix, hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and delivery. We developed a industrialscale manufacturing process of a high-purity and virus-safe hyaluronidase from ovine testis. The purification process contains blue-sepharose affinity chromatography, mono S cation-exchange chromatography, heparin sepharose affinity chromatography, and virus filtration process. In order to evaluate the efficacy of blue sepharose chromatography, heparin sepharose chromatography, and virus filtration process for the removal of possible contaminating viruses during the manufacture of hyaluronidase, a variety of experimental model viruses including ovine visna virus, bovine herpesvirus, bovine parainfluenza virus 3, and bovine parvovirus were chosen. All the viruses tested were completely removed during virus filtration process. Also all the viruses were effectively separated from hyaluronidase during blue sepharose chromatography and heparin sepharose chromatography. These results indicate that the manufacturing process for hyaluronidase from sheep testis has sufficient virus-reducing capacity to achieve a high margin of virus safety. http://dx.doi.org/10.1016/j.nbt.2016.06.973
P3-5 Fruit juices as a new source of probiotics in a lactose free diet Anna Kostecka-Gugała 1,∗ , Aleksandra Mech-Nowak 2 , Aleksandra Duda-Chodak 3 , Paweł Kaszycki 1
P3-6 Phosphorothioate analogues of lysophosphatidylcholine as signaling molecules for type 2 diabetes-related membrane receptors Maria Koziołkiewicz ∗ , Anna Drzazga, Edyta Gendaszewska-Darmach Lodz University of Technology, Poland During last two decades it has been evidenced that lysophospholipids (LPLs) are important signaling molecules involved in many physiological and pathophysiological processes. They contain a glycerol backbone with one fatty acid residue ester-linked at the sn-1 or sn-2 position and a phosphate residue at the sn-3 position as a part of hydrophilic “head”. To the best characterized LPLs belong sphingosine-1-phosphate, lysophosphatidic acids (LPAs) and cyclic phosphatidic acids (cPAs) structurally related to LPAs. However, recent studies indicate that also lysophosphatidylcholine (LPC) is an important signaling molecule capable of modulation of insulin secretion. Our studies have been focused on phosphorothioate LPC analogues containing sulfur atoms and various acyl residues and their ability to activate two main GPCR receptors related to insulin secretion: GPR119 and GPR55. Structural differences between native LPCs and their synthetic analogues have a great impact on both the strength and manner of the influence on in vitro  cell models, including activation of different signaling pathways (cAMP synthesis and Ca2+ release) and, what is the most important, glucose-stimulated insulin secretion.
1
Institute of Plant Biology and Biotechnology, Poland Faculty of Biotechnology and Horticulture, Poland 3 University of Agriculture in Krakow, Poland 2
Probiotic bacteria are responsible for a variety of health benefits such as maintenance of individual intestinal microbiota during antibiotic treatment, support of the immune system, decreased threat of colonization with potentially pathogenic gastrointestinal microorganisms, protection of proteins and lipids against oxidative damage, and even help to relieve depression or prevent obesity.
This project is supported by the Polish National Science Centre (NCN, grant no. 2013/11/N/NZ5/00270). http://dx.doi.org/10.1016/j.nbt.2016.06.975