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Functional arrangement of cone photorecptors in the dace retina
s122
19. Visual system FUNCI’IONAL
ARRANGEMENT
OF CONE PHOTORBCPTORS
YOKO HASHIMOX)‘, SOH HIDAKA’ AND TERUYA OHTSvK~2, 8-1 Kawada-cho, Shiniuku-ku, Tokyo 162, 2Deuartment Sciences, Myodaiii, Okazaki, Aichi 444, Japan.
IN THE DACB RETINA of Physiology.
‘Department
of Information
Physiology,
Tokyo Women’s Medical College,
National
Institute for Physiological
Regular arrangement of cone photoreceptors can be seen in the retina of some teleosts. In order to find the functional significance of such regularity in the retina, we investigated the mosaic of cones in the Japanese date (Tribolodon hakonensis). The date has six morphological subtypes of cones; 4 single cones and two members of double cones. Each of them contains one of four different kinds of visual pigments, i.e., red, green, blue, and UV absorbing visual pigments (Harosi and Hashimoto, 1983). In the present study, we investigated the mosaic of different chromatic subtypes of cones by immunohistochemical labeling. The antisera we used recognize different cone opsins. The antibody against green cone opsin labeled one subtype of single cones as well as accessory member of double cone. They arranged in a rectangular manner. We further examined the synaptic endings of these cones by electron microscopy and found that their terminals keep the similar mosaic pattern to that of cone outer segment. Our finding suggests that the spatial information of chromatic signal in the outer segment is conveyed to the synaptic ending of cone photoreceptors.
SIMULATION RAL
STUDY
NETWORKS,
Computer
Sciences,
Typical
color
mapping chromatic
AND
SHIRO
AND
through
multilayered
responses,
neural
through
one of the three
crimination
ability
the mechanism.
under
acquired
network
defined
SHIGEKI
models
the constraint
with
and/or
types
that
suggest
that
predicted
the hypothesis
Dichromatic
light,
the experimental for constructing
blindness
In this study,
which
realize
we
nonlinear
by one luminance
color vision
and two
is represented
by
has the best color dis-
was completed, wavelength
evidence
and
441, Japan.
of color
models.
color vision
After learning
of spectral
Toyohashi
color vision,
color space defined
NEU-
Information
and the nature
by color vision
the dichromatic
of a loss of one type of cones.
Each model
of cones,
method.
of
Tempaku,
and dichromatic
learning
BY MULTILAYERED
Department
Hibarigaoka
into perceived
hypothesis
VISION
,
predicted
such as hue representation
were examined. results
l-l
for normal
the back-propagation cone units
COLOR
NAKAUCHI
of loss of some
experiments
by cone responses,
by learning,
These
DICHROMATIC
of Technology,
is a consequence
eliminating
color representation,
University
psychophysical
from cone space
of each model
USUI
Toyohashi
blindness
has been studied constructed
OF NORMAL
well enough
the dichromatic
the properties
discrimination
and
to understand
color vision
model
is
reasonable.
L-ARGININE:NITRIC
0XIDE:cGMP PATHWAY IN RETINAL HORIZONTAL CELLS.
EI-ICHI MIYACHI,
Department of Physiology,
Fujita Health University
School of
Medicine, Toyoake, Aichi 470-11, Japan. At turtle
the 14th Annual Meeting injection
retinae,
decoupling
of
dibutyryl cGMP
of gap junctions between the cells.
when L-arginine was injected into the cells. of
we reported that,
(Kyoto, 1990),
horizontal
injections.
cells The
around
at 0 mV.
nitric
oxide
could
reversal
be
easily
into
horizontal
Similar results
in
carp
cells were
obtained
After the injection, all the types
polarized
by
intracellular
current
potentials of the light responses were estimated known as the specific inhibitor of the
N-Methyl-L-arginine,
synthase,
suppressed the effect of
L-arginine.
These
results
suggest the presence of a possible regulating mechanism of gap junctions by L-arginine:nitric
and
caused
oxide:cGMP pathway in retinal horizontal cells.
the
19. Visual system FUNCI’IONAL
ARRANGEMENT
OF CONE PHOTORBCPTORS
YOKO HASHIMOX)‘, SOH HIDAKA’ AND TERUYA OHTSvK~2, 8-1 Kawada-cho, Shiniuku-ku, Tokyo 162, 2Deuartment Sciences, Myodaiii, Okazaki, Aichi 444, Japan.
IN THE DACB RETINA of Physiology.
‘Department
of Information
Physiology,
Tokyo Women’s Medical College,
National
Institute for Physiological
Regular arrangement of cone photoreceptors can be seen in the retina of some teleosts. In order to find the functional significance of such regularity in the retina, we investigated the mosaic of cones in the Japanese date (Tribolodon hakonensis). The date has six morphological subtypes of cones; 4 single cones and two members of double cones. Each of them contains one of four different kinds of visual pigments, i.e., red, green, blue, and UV absorbing visual pigments (Harosi and Hashimoto, 1983). In the present study, we investigated the mosaic of different chromatic subtypes of cones by immunohistochemical labeling. The antisera we used recognize different cone opsins. The antibody against green cone opsin labeled one subtype of single cones as well as accessory member of double cone. They arranged in a rectangular manner. We further examined the synaptic endings of these cones by electron microscopy and found that their terminals keep the similar mosaic pattern to that of cone outer segment. Our finding suggests that the spatial information of chromatic signal in the outer segment is conveyed to the synaptic ending of cone photoreceptors.
SIMULATION RAL
STUDY
NETWORKS,
Computer
Sciences,
Typical
color
mapping chromatic
AND
SHIRO
AND
through
multilayered
responses,
neural
through
one of the three
crimination
ability
the mechanism.
under
acquired
network
defined
SHIGEKI
models
the constraint
with
and/or
types
that
suggest
that
predicted
the hypothesis
Dichromatic
light,
the experimental for constructing
blindness
In this study,
which
realize
we
nonlinear
by one luminance
color vision
and two
is represented
by
has the best color dis-
was completed, wavelength
evidence
and
441, Japan.
of color
models.
color vision
After learning
of spectral
Toyohashi
color vision,
color space defined
NEU-
Information
and the nature
by color vision
the dichromatic
of a loss of one type of cones.
Each model
of cones,
method.
of
Tempaku,
and dichromatic
learning
BY MULTILAYERED
Department
Hibarigaoka
into perceived
hypothesis
VISION
,
predicted
such as hue representation
were examined. results
l-l
for normal
the back-propagation cone units
COLOR
NAKAUCHI
of loss of some
experiments
by cone responses,
by learning,
These
DICHROMATIC
of Technology,
is a consequence
eliminating
color representation,
University
psychophysical
from cone space
of each model
USUI
Toyohashi
blindness
has been studied constructed
OF NORMAL
well enough
the dichromatic
the properties
discrimination
and
to understand
color vision
model
is
reasonable.
L-ARGININE:NITRIC
0XIDE:cGMP PATHWAY IN RETINAL HORIZONTAL CELLS.
EI-ICHI MIYACHI,
Department of Physiology,
Fujita Health University
School of
Medicine, Toyoake, Aichi 470-11, Japan. At turtle
the 14th Annual Meeting injection
retinae,
decoupling
of
dibutyryl cGMP
of gap junctions between the cells.
when L-arginine was injected into the cells. of
we reported that,
(Kyoto, 1990),
horizontal
injections.
cells The
around
at 0 mV.
nitric
oxide
could
reversal
be
easily
into
horizontal
Similar results
in
carp
cells were
obtained
After the injection, all the types
polarized
by
intracellular
current
potentials of the light responses were estimated known as the specific inhibitor of the
N-Methyl-L-arginine,
synthase,
suppressed the effect of
L-arginine.
These
results
suggest the presence of a possible regulating mechanism of gap junctions by L-arginine:nitric
and
caused
oxide:cGMP pathway in retinal horizontal cells.
the