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Fundamentals of thin layer chromatography (planar chromatography), 2nd edn.
worst being the confusion of emission and excitation in the captions of Figs. 2.5b and c. It is also off-putting to your reviewer to be told that fluorescence was first observed as long ago as 1565 when he is aware that the first text on the subject was by Gesner in 1555, and that correcting for curvature of calibration curves necessarily improves accuracy of results. Leaving aside the minor blemishes and points for discussion this is a most welcome and excellent introduction to the subject areas. D. Thorburn
Burns
W.R. Croasmun and R.M.K. Carlson (Eds. ), Two-DimensicGi& NMR Spectroscopy: Applications for Chemists and Biochemists (Methods in Stereochemical Analysis, Vol. 9). VCH, Weinheim, 1987 (ISBN 3-527-26528-7). 511 pp. Price DM 197.00. This book is mainly directed at chemists and biochemists who use NMR spectroscopy but are not yet familiar with 2D NMR techniques. In particular it will interest those who are involved in the structural elucidation of complex organic compounds. The text provides a useful collection of well explained and illustrated examples of the application of 2D NMR methods. One-dimensional spectra which have been uninterpretable due to extensive overlapping signals, even with the use of high field spectrometers, can now be tackled using a variety of new 2D techniques provided the NMR spectrometer can be programmed with the appropriate pulse sequences. The range of compounds covered in the text include the cyclic peptide cyclosporin, DNA, oligosaccharides, several steroids, the polycyclic compound spatol, terpenes, an alkaloid and a polycyclic aromatic hydrocarbon. The interpretations of the spectra are given, as well as detailed advice on the best strategy for tackling each structural elucidation. The book begins with three comprehensive chapters covering the theoretical, experimental and common strategies. Novices to the 2D NMR techniques may find the first part of the book difficult to comprehend, in which case the later applications chapters could provide a more rewarding introduction to this subject. John C. Tebby Fredrick Geiss, Fundamentals of Thin Layer Chromatography (Planar Chromatography), 2nd edn. Huthig, Heidelberg, 1987 (ISBN 3-7785-0854-7). xviii + 482 pp. Price DM 192.00. This book fills a gap in the English language text book publications on TLC. Both the first and this second edition are aimed to bridge the gap between the numerous laboratory handbooks and the all too few fundamental theoretical
366
texts. This is an excellent, readable and interesting scholarly account of both theory and practice. Although it is not meant as a theoretical approach text it leans that way since it describes what happens, why it happens and how the analyst can, within reason, control what happens. The topics discussed include the relative strengths and weaknesses of various chromatographic procedures, solvent flow and layer quality, solvents and their activity, the solvent/gas spacelayer, effects of temperature and other factors of influence on separation, gradients, and the chromatographic result including quantitative measurement and evaluation. The transfer of TLC separations to columns is dealt with via a transfer equation. The general suggestions for practical work start with the ten commandments for the TLC analyst and conclude with a most useful decision flow chart. This is an essential purchase for all third level institution teachers of analytical science and their institutional libraries. D. Thorburn
Burns
MS. Verrall and M.J. Hudson (Eds.), Separations for Biotechnology. Horwood, Chichester, 1987 (ISBN O-7458-0090-4). 502 pp. Price g55.00. This book is one in a series on Biochemistry and Biotechnology and contains many of the papers presented at an international conference on downstream processing in biotechnology which was held at the University of Reading in September 1987. It has therefore been published commendably quickly after the conference and consists of forty camera-ready presentations and a reasonable index. There has been a growing interest in biotechnology in recent years, and one of the key areas for development has been downstream separation of the desired product from highly complex mixtures. These conference proceedings cover six aspects of downstream processing; namely cell harvesting and disruption, adsorption and chromatography, liquid-liquid extraction, analytical techniques and process control, and product containment and safety. The proceedings are of interest to analytical chemists in that they discuss a challenging area of separation science. The section on analytical techniques is concerned primarily with process control applications using not only chromatography, but also optical and electrochemical methods. R.J.H. Clark and R.E. Hester (Eds.), Advances in Non-Linear Spectroscopy. Wiley, Chichester, 1988 (ISBN O-471-91652-8). xix + 363 pp. Price g70.00. This book is intended to provide a survey of the current state of non-linear Raman spectroscopy, and forms Vol. 15 of the Advances in Spectroscopy series. The seven chapters describe coherent Raman spectroscopy of gases, Raman amplification spectroscopy, high resolution CARS and inverse Raman spectroscopy, polarization CARS spectroscopy, quantitative CARS spectroscopy,
Burns
W.R. Croasmun and R.M.K. Carlson (Eds. ), Two-DimensicGi& NMR Spectroscopy: Applications for Chemists and Biochemists (Methods in Stereochemical Analysis, Vol. 9). VCH, Weinheim, 1987 (ISBN 3-527-26528-7). 511 pp. Price DM 197.00. This book is mainly directed at chemists and biochemists who use NMR spectroscopy but are not yet familiar with 2D NMR techniques. In particular it will interest those who are involved in the structural elucidation of complex organic compounds. The text provides a useful collection of well explained and illustrated examples of the application of 2D NMR methods. One-dimensional spectra which have been uninterpretable due to extensive overlapping signals, even with the use of high field spectrometers, can now be tackled using a variety of new 2D techniques provided the NMR spectrometer can be programmed with the appropriate pulse sequences. The range of compounds covered in the text include the cyclic peptide cyclosporin, DNA, oligosaccharides, several steroids, the polycyclic compound spatol, terpenes, an alkaloid and a polycyclic aromatic hydrocarbon. The interpretations of the spectra are given, as well as detailed advice on the best strategy for tackling each structural elucidation. The book begins with three comprehensive chapters covering the theoretical, experimental and common strategies. Novices to the 2D NMR techniques may find the first part of the book difficult to comprehend, in which case the later applications chapters could provide a more rewarding introduction to this subject. John C. Tebby Fredrick Geiss, Fundamentals of Thin Layer Chromatography (Planar Chromatography), 2nd edn. Huthig, Heidelberg, 1987 (ISBN 3-7785-0854-7). xviii + 482 pp. Price DM 192.00. This book fills a gap in the English language text book publications on TLC. Both the first and this second edition are aimed to bridge the gap between the numerous laboratory handbooks and the all too few fundamental theoretical
366
texts. This is an excellent, readable and interesting scholarly account of both theory and practice. Although it is not meant as a theoretical approach text it leans that way since it describes what happens, why it happens and how the analyst can, within reason, control what happens. The topics discussed include the relative strengths and weaknesses of various chromatographic procedures, solvent flow and layer quality, solvents and their activity, the solvent/gas spacelayer, effects of temperature and other factors of influence on separation, gradients, and the chromatographic result including quantitative measurement and evaluation. The transfer of TLC separations to columns is dealt with via a transfer equation. The general suggestions for practical work start with the ten commandments for the TLC analyst and conclude with a most useful decision flow chart. This is an essential purchase for all third level institution teachers of analytical science and their institutional libraries. D. Thorburn
Burns
MS. Verrall and M.J. Hudson (Eds.), Separations for Biotechnology. Horwood, Chichester, 1987 (ISBN O-7458-0090-4). 502 pp. Price g55.00. This book is one in a series on Biochemistry and Biotechnology and contains many of the papers presented at an international conference on downstream processing in biotechnology which was held at the University of Reading in September 1987. It has therefore been published commendably quickly after the conference and consists of forty camera-ready presentations and a reasonable index. There has been a growing interest in biotechnology in recent years, and one of the key areas for development has been downstream separation of the desired product from highly complex mixtures. These conference proceedings cover six aspects of downstream processing; namely cell harvesting and disruption, adsorption and chromatography, liquid-liquid extraction, analytical techniques and process control, and product containment and safety. The proceedings are of interest to analytical chemists in that they discuss a challenging area of separation science. The section on analytical techniques is concerned primarily with process control applications using not only chromatography, but also optical and electrochemical methods. R.J.H. Clark and R.E. Hester (Eds.), Advances in Non-Linear Spectroscopy. Wiley, Chichester, 1988 (ISBN O-471-91652-8). xix + 363 pp. Price g70.00. This book is intended to provide a survey of the current state of non-linear Raman spectroscopy, and forms Vol. 15 of the Advances in Spectroscopy series. The seven chapters describe coherent Raman spectroscopy of gases, Raman amplification spectroscopy, high resolution CARS and inverse Raman spectroscopy, polarization CARS spectroscopy, quantitative CARS spectroscopy,