Ganglioside pattern of rat astrocyte sub-cultures

435

50

GANGLIOSIDE

PATTERN

M. MERSEL,

OF RAT ASTROCYTE

G. REBEL & P. MANDEL

SUB-CULTURES

- Strasbourg,

France

In contrast to mouse and chicken astrocytes the ganglioside pattern of cultured rat We have obtained apparently a line of rat astrocytes is usually less differentiated. astrocytes probably through a slight spontaneous transformation. The doubling-time of this cultures is 24 hours while that of primary cultures of rat astrocytes is 36 hours in similar conditions. After 40 generations the specific glial fibrillar acidic protein is still present in our astrocyte cultures as demonstrated by immunocytochemistry. A ganglioside pattern of differentiated cells with the presence of di- and tetra-sialogangliosides was obtained. (Isolation and analysis of gangliosides were performed according to R.W. Ledeen & R.K. Yu, Methods in enzymology, 1982, 83, PP 139).

51 INFLUENCE

OF N-ISOPROPYLAMINO-2-PYRIMIDINE

Ciccarelli Dept.

R.;

Di Iorio

P.;

of Pharmacology.

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substances

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