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Gel permeation and ion-exchange chromatography of proteins and peptides
472
Journal of Chromatography, 380 (1986) 472-473 Biomedical Applications Elsevier Science Publishers B.V., Amsterdam - Printed in The Netherlands
CHROMBIO. 3155
Book Review Gel permeation and ion-exchange chromatography of proteins and peptides, edited by H. Parvez, Y. Kato and S. Parvez, VNU Science Press, Utrecht, 1985, X + 224 pp., price DM 139.00, ISBN 90-6764-048-4. The aims of this book are high and the topic appears most attractive, one in which every effort is made to use the methods of separation in order to increase human knowledge on protein structure and function. Personally disliking VoIUminous books, I wondered how such a broad topic could be squeezed into the quoted 224 pages. The book consists of a number of papers written mostly by Japanese authors. Some of these articles resemble lectures, but most of them bear all the features of a primary publication. Therefore, those readers expecting broad overviews will be disappointed, while those who are looking for a particular type of separation may find what they need here. Bearing this profile in mind, it is unlikely that the volume will serve as a valuable reference in protein analysis, as stated by the editors in the Introduction. In short, the book resembles a monotopical issue of a leading chromatographic journal and the question arises as to why the authors have not published these results in a journal where they would certainly fit and where they are likely to find a broader audience. The other problem is that of arranging the individual contributions. In such a volume, one would expect papers on technical aspects at the beginning, followed by individual application papers. Just the reverse is true here. There is no system in the application papers whatsoever; they span over the separations of glycoproteins, lipoproteins, enzymes, cytochromes and receptors, to enumerate just a few of the topics of some of the contributions. There are some chapters that are good, concise and informative, exactly what one would expect in a book of this type. These are, notably: Determination of protein molecular weight by gel permeation chromatography (T. Takagi), Gel permeation of proteins by high-performance gel chromatography in denaturing solvents (K. Konishi), Enzyme purification by high-performance ion-exchange liquid chromatography (F.B. Rudolph, B.F. Cooper and J. Greenhut) and Technical aspects of biochemical high-performance column liquid chromatography (J. Sjsdahl et al.). What puzzles me is why the first chapter of a book devoted to protein separations does not deal with proteins at all (it deals with gel permeation chromatography of nur ic acids). This is even more surprising when one realizes that this chapter is written by the editors of the volume.
473
In summary, this is an unbalanced multiauthor book which has some chapters that are worth reading as review articles and others that bring detailed information from the application area. In my view, it should have been stated somewhere that the whole is intended as a periodicurn, bringing together original articles and mini-reviews from a particular application area. If judged as monograph (as one is tempted to do after having read the Introduction and the Preface), the result is a failure.
[email protected]! (CzechoeEouahia)
Journal of Chromatography, 380 (1986) 472-473 Biomedical Applications Elsevier Science Publishers B.V., Amsterdam - Printed in The Netherlands
CHROMBIO. 3155
Book Review Gel permeation and ion-exchange chromatography of proteins and peptides, edited by H. Parvez, Y. Kato and S. Parvez, VNU Science Press, Utrecht, 1985, X + 224 pp., price DM 139.00, ISBN 90-6764-048-4. The aims of this book are high and the topic appears most attractive, one in which every effort is made to use the methods of separation in order to increase human knowledge on protein structure and function. Personally disliking VoIUminous books, I wondered how such a broad topic could be squeezed into the quoted 224 pages. The book consists of a number of papers written mostly by Japanese authors. Some of these articles resemble lectures, but most of them bear all the features of a primary publication. Therefore, those readers expecting broad overviews will be disappointed, while those who are looking for a particular type of separation may find what they need here. Bearing this profile in mind, it is unlikely that the volume will serve as a valuable reference in protein analysis, as stated by the editors in the Introduction. In short, the book resembles a monotopical issue of a leading chromatographic journal and the question arises as to why the authors have not published these results in a journal where they would certainly fit and where they are likely to find a broader audience. The other problem is that of arranging the individual contributions. In such a volume, one would expect papers on technical aspects at the beginning, followed by individual application papers. Just the reverse is true here. There is no system in the application papers whatsoever; they span over the separations of glycoproteins, lipoproteins, enzymes, cytochromes and receptors, to enumerate just a few of the topics of some of the contributions. There are some chapters that are good, concise and informative, exactly what one would expect in a book of this type. These are, notably: Determination of protein molecular weight by gel permeation chromatography (T. Takagi), Gel permeation of proteins by high-performance gel chromatography in denaturing solvents (K. Konishi), Enzyme purification by high-performance ion-exchange liquid chromatography (F.B. Rudolph, B.F. Cooper and J. Greenhut) and Technical aspects of biochemical high-performance column liquid chromatography (J. Sjsdahl et al.). What puzzles me is why the first chapter of a book devoted to protein separations does not deal with proteins at all (it deals with gel permeation chromatography of nur ic acids). This is even more surprising when one realizes that this chapter is written by the editors of the volume.
473
In summary, this is an unbalanced multiauthor book which has some chapters that are worth reading as review articles and others that bring detailed information from the application area. In my view, it should have been stated somewhere that the whole is intended as a periodicurn, bringing together original articles and mini-reviews from a particular application area. If judged as monograph (as one is tempted to do after having read the Introduction and the Preface), the result is a failure.
[email protected]! (CzechoeEouahia)