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Genetic enhancement of Murray cod for aquaculture and conservation
Abstracts / Aquaculture 272S1 (2007) S238–S321
to 100% among groups reared in the aquariums without MT feeding (WE), but significant differences between treatments were not observed. Significant differences were observed (P b 0.05) in the frequency of inverted males within (MT) and (WE) groups and in comparison with their control. Also insignificant differences (P N 0.05) were observed in the frequency of females and intersexes within (MT) and (WE) groups. It can be concluded that water contaminated with MT can be used for successful masculinization of females in common carp. doi:10.1016/j.aquaculture.2007.07.092
Mapping of candidate genes for sex determination in tilapias A. Shirak a, E. Seroussi a, N. Zilberman a, R. Domokhovsky a, A. Cnaani b, T.D. Kocher b, M. Ron a , G. Hulata a a Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel b Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA Expression dynamics of gonad differentiation regulators is similar between zebrafish and mammals. This recent observation prompts us to study candidates for the role of master key regulators (MKRs) in tilapias. We generated genetic markers in 11 putative genes. We genotyped these markers in two purebred species – Oreochromis aureus and O. niloticus – and mapped them to the tilapia linkage groups using genotypic data of 90 individuals of the F2 mapping family. The mapping position of Dax1 connected LG16 and LG21, separated until now, into a single linkage group. The mapping positions of two other candidates overlapped with the mapping positions of two previously reported QTL regions for sex determination. The rest of the genes were mapped to linkage groups 1, 12, 14, and 17. doi:10.1016/j.aquaculture.2007.07.093
Genetic enhancement of Murray cod for aquaculture and conservation B.A. Ingram a, H. McPartlan b, M. Rourke b, W. Bravington a, N. Robinson b, B. Hayes b
S271
a
Department of Primary Industries, PIRVic Snobs Creek, Private Bag 20, Alexandra, VIC. Australia 3714 b Department of Primary Industries, PIRVic Attwood, 475 Mickleham Road, Attwood, VIC., Australia, 3049 Aquaculture of Murray cod (Maccullochella peelii peelii), an Australian freshwater species, is a new industry which has the potential to expand both domestically and internationally. The species is highly valued and suited to large-scale production, with promising performance for growth, survival and feeding efficiencies at high densities. Until now, no active selection of fish has taken place for aquaculture purposes with the majority of seedstock being derived directly from wild broodstock held in captivity. Consequently, there is considerable potential to increase performance and profitability through instigation of a selective breeding program. A selective breeding program has been initiated for Murray cod that will apply innovative genetic and reproduction technologies to enhance profitability and sustainability of aquaculture as well as to protect biodiversity of wild stocks. Progeny from 50 separate matings have been procured as potential future broodfish and traits of interest for selection have been identified. Progeny from four families have been retained for a gene mapping program. These families will be screened using in excess of 100 newly identified microsatellite markers, which will be used to discover DNA markers that are linked to desirable traits. These markers will then be used to assist in the selection of future broodstock. The microsatellite markers will also be used to determine the genetic structure of historic and contemporary wild populations. Reproduction technologies are also being explored to enhance production and associated performance of fish from the selective breeding program. These include controlled reproduction, chromosome-set manipulation, inter-specific hybridisation and sperm cryopreservation. doi:10.1016/j.aquaculture.2007.07.094
Genetic characterisation of cultured and natural-water populations of common carp (Cyprinus carpio) in Hungary I. Lehoczky a, Z.T. Nagy b, I. Magyary a, C. Hancz a, J. Bakos b, Z. Jeney b a University of Kaposvar, Department of Animal Sciences H-7400 Kaposvar Guba S. u. 1., Hungary
to 100% among groups reared in the aquariums without MT feeding (WE), but significant differences between treatments were not observed. Significant differences were observed (P b 0.05) in the frequency of inverted males within (MT) and (WE) groups and in comparison with their control. Also insignificant differences (P N 0.05) were observed in the frequency of females and intersexes within (MT) and (WE) groups. It can be concluded that water contaminated with MT can be used for successful masculinization of females in common carp. doi:10.1016/j.aquaculture.2007.07.092
Mapping of candidate genes for sex determination in tilapias A. Shirak a, E. Seroussi a, N. Zilberman a, R. Domokhovsky a, A. Cnaani b, T.D. Kocher b, M. Ron a , G. Hulata a a Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel b Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA Expression dynamics of gonad differentiation regulators is similar between zebrafish and mammals. This recent observation prompts us to study candidates for the role of master key regulators (MKRs) in tilapias. We generated genetic markers in 11 putative genes. We genotyped these markers in two purebred species – Oreochromis aureus and O. niloticus – and mapped them to the tilapia linkage groups using genotypic data of 90 individuals of the F2 mapping family. The mapping position of Dax1 connected LG16 and LG21, separated until now, into a single linkage group. The mapping positions of two other candidates overlapped with the mapping positions of two previously reported QTL regions for sex determination. The rest of the genes were mapped to linkage groups 1, 12, 14, and 17. doi:10.1016/j.aquaculture.2007.07.093
Genetic enhancement of Murray cod for aquaculture and conservation B.A. Ingram a, H. McPartlan b, M. Rourke b, W. Bravington a, N. Robinson b, B. Hayes b
S271
a
Department of Primary Industries, PIRVic Snobs Creek, Private Bag 20, Alexandra, VIC. Australia 3714 b Department of Primary Industries, PIRVic Attwood, 475 Mickleham Road, Attwood, VIC., Australia, 3049 Aquaculture of Murray cod (Maccullochella peelii peelii), an Australian freshwater species, is a new industry which has the potential to expand both domestically and internationally. The species is highly valued and suited to large-scale production, with promising performance for growth, survival and feeding efficiencies at high densities. Until now, no active selection of fish has taken place for aquaculture purposes with the majority of seedstock being derived directly from wild broodstock held in captivity. Consequently, there is considerable potential to increase performance and profitability through instigation of a selective breeding program. A selective breeding program has been initiated for Murray cod that will apply innovative genetic and reproduction technologies to enhance profitability and sustainability of aquaculture as well as to protect biodiversity of wild stocks. Progeny from 50 separate matings have been procured as potential future broodfish and traits of interest for selection have been identified. Progeny from four families have been retained for a gene mapping program. These families will be screened using in excess of 100 newly identified microsatellite markers, which will be used to discover DNA markers that are linked to desirable traits. These markers will then be used to assist in the selection of future broodstock. The microsatellite markers will also be used to determine the genetic structure of historic and contemporary wild populations. Reproduction technologies are also being explored to enhance production and associated performance of fish from the selective breeding program. These include controlled reproduction, chromosome-set manipulation, inter-specific hybridisation and sperm cryopreservation. doi:10.1016/j.aquaculture.2007.07.094
Genetic characterisation of cultured and natural-water populations of common carp (Cyprinus carpio) in Hungary I. Lehoczky a, Z.T. Nagy b, I. Magyary a, C. Hancz a, J. Bakos b, Z. Jeney b a University of Kaposvar, Department of Animal Sciences H-7400 Kaposvar Guba S. u. 1., Hungary