- Email: [email protected]
Gestational changes in endothelium-dependent relaxation (EDR) of rat aorta
130 to their lives. Only two women said that maybe it would be better for them if they had their first child not so early. All the others had a positive view of having children early. Conclusions: The author conclude that at least to a significant extent, the onsuccess of many teens’pregnancy prevention programs can be associated to a deep disagreement between the doctors’ and the adolescents’ perceptions of motherhood in adolescence and that it could be helpful if the doctors considered the womens’ point of view, before planning strategies towards their health.
P1.14.20 ACUTE URINARY RETENTION SECONDARY TO AN IMPERFORATE HYMEN: A CASE REPORT MS. Arruda’, D.B. Tianoz. lOb/Gyn Dept., Hospital EvangClico Samaritan0 de Campinas, Campinas, SBo Paula - Brazil; 20biGyn Dept., Pontificia Universidade Catdlica de Campinas, Campinas, SBo Paula, Brazil. Objective: Report a case of acute urinary retention in an adolescent girl with hematometrocolpos causes by imperforate hymen. Study Methods: Case report Results: A healthy 12.year-old girl with a three day history of pelvic discomfort and urinary retention, had had three appointments to a pediatric emergency unit for 3 consecutive days, and diagnosed as having urinary retention. Bladder drainage by means of urethral catheter in all three visits was performed: approximately 700 ml of urine each time. On her fourth visit to the hospital, she presented again with a large volume urinary retention and important pelvic pain. She was referred to the gynecology department for evaluation. Telarche at 10 years old (Tanner II), and pubarche eight month later (Tanner II). No history of vaginal bleeding. The over distended bladder was palpable just above the umbilicus. Inspection of the vulva revealed a dome-shaped, purplishred hymenal membrane, bulging outward. On rectal examination, the vagina was palpable as a large cystic mass. The bladder was catheterized and 1500 ml of urine were drained. Ultrasound revealed a haematocolpos and haematometrium. Hymenotomy with cruciate incision was performed and 300 ml of collected blood was promptly released. Two stitches kept the central part of the hymenal membrane apart in order to avoid coalescence. Bladder catheter was removed right after the procedure and spontaneous voiding occurred five hours later. Patient was discharged in 12 hours and recovery was uneventful. Conclusions: The accumulation of the menstrual blood in the vagina and uterus may form a mechanical effect on the urethra and bladder and lead to the obstructive urinary symptoms. The impeforate hymen is a rare gynecologic abnormality that may not be detected until the onset of menses, and must be suspected in young adolescents with pelvic pain or urinary retention who has not experienced menarche.
P1.14.21 AN INVERSE RELATION BETWEEN MENARCHEAL AGE AND BODY FAT MASS AMOUNT DURING ADULTHOOD T.Douchi, M.Nakae, S.Yamamoto, Y.Nagata, Kagoshima University Hospital, 8-35-l Sakuragaoka, Kagoshima, Japan, 890-8520. Objectives: The purpose of the present study was to investigate the relation of age at menarche to adult body fat and lean mass. Study Methods: One hundred eighty-three premenopausal healthy Japanese women aged 20 to 39 years were enrolled. Baseline characteristics included age, age at menarche, and height. Body fat mass, the ratio of trunk fat mass to leg fat mass (trunk-leg fat ratio), and body lean mass were measured by dual-energy x-ray absorptiometry. Correlation of body fat or lean mass with baseline characteristics were investigated by univariate and multiple regressio analysis. Results: Age at menarche inversely correlated with adult body fat mass (r = -0.21, P < O.Ol), and tended to inversely correlate with adult body lean mass (r = -0.14, P = 0.06). However, age at menarche did not correlate with adult trunk-leg fat ratio (r = -0.11, P = 0.14). Age at menarche correlated with body fat mass, independent of age and height on multiple regression analysis (P < 0.01). Conclusions: Body fat mass amount rather than body fat distribution and body lean mass during adulthood is inversely correlated with menarcheal age, suggesting that increase in fat mass amount is a late event (i.e. onset of menarche) in the pubertal process.
MONDAY,
SEPTEMBER
P1.14.22 FREQUENCY OF MAMMARIAN DISTURBANCES IN INFANTILE AND JUVENILE POPULATION IN CUBA: THREE YEARS OF EXPERIENCE J. 0. Rodriguez, 0. R. Bermtidez, National Commission for Infantile and Juvenile Gynecology, Ministry of Public Health, Havana, Cuba. Objective: To determine the principal presenting complaints and frequency of mammary illnesses in a pediatric and adolescent urban population in Cuba. Study Methods: A prospective, longitudinal and descriptive study was carried out in 100% of the patients that assisted to infantile and juvenile gynecology consultation due to mammary problems in Havana from January 1996 to December 1998. Participant gynecologists had beforehand special training in issues related to pediatric and adolescent gynecology and they are members of the Cuban Program for the Development of Pediatric and Adolescent Reproductive Health. This program coordinated its efforts with the Cuban primary health system through its network of family doctors (coverage: 120 families/physician). This system identifies health problems by a prophylactic screening of sub-populations with risk factors, as is the infantile and adolescent population, and then refers cases to specialized consultations. Results: In total, 22,662 young women were attended at the infantile and juvenile gynecology consultations during the period of the study, an important number as self-referrals. 1103 of them required gynecologic care due to mammary disturbances (4.86%) with a higher prevalence in the adolescent years (5.29%) compared to girls younger than 10 years of age (3.87%). The most frequent diagnosis in young girls was normal puberty (39.8%), premature telarchia (39.5%) and precocious telarchia (7.9%). Common diagnoses in adolescents were normal breast development (31.3%), mastodiniainipple discharge (25.2%), fibrocystic disease (15.2%) and developmental-related complaints. Among the latter, (53.5%) and virginal hypertrophy (41.2%) were the most common. Overall, the most frequent presenting complaints were those related to puberal developmental disorders and with normal mammary gland growth. Conclusions: The most frequent presenting complaints in our population were those related to puberal developmental disorders and with normal mammary gland growth. A plausible explanation for this is the relatively high frequency of self-referral cases that existed after the system begun its function.
P1.15
PHYSIOLOGY
OF REPRODUCTION
1
Pl.lS.01 GESTATIONAL CHANGES IN ENDOTHELIUM-DEPENDENT RELAXATION (EDR) OF RAT AORTA S. Sakamoto (l), A. Wakabayashi (l), M. Harada (l), A. Nagai (l), T. Aso( M. Goto (2), H. Azuma (2) (1) Dept. OBIGYN, Tokyo Medical and Dental University, Tokyo, Japan (2) Inst. Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan Objective: The present experiments were designed to investigate the gestational changes in endothelium-dependent relaxation (EDR) of rat aorta. Study Methods: Thoracic aortas were taken from non-pregnant 7, 14 and 21 days pregnant SD rats and 2mm width transverse strips with endothelium [E (+)] or without endothelium [E (-)] were made. After tonic contraction was induced by 10 -‘M U46619, EDR induced by acetylcholine (ACh) (109-3x10~5 M) in E (+) strips and relaxation in response to sodium nitroprusside (SNP) (109-3x10~5 M) in E (-) strips were observed. Results: In E (+) strips, ACh-induced EDR was unchanged at 7 days pregnancy and augmented at 14 and 21 days pregnancy when comparison was made on the non-pregnancy. In E (-) strips, SNPinduced relaxation was unchanged at 7 and 14 days pregnancy and augmented at 21 days pregnancy when compared with that at nonpregnancy. Basal and ACh-stimulated c-GMP levels in aorta were augmented at 14 days pregnancy and unchanged at 21 days pregnancy when compared with those at non-pregnancy.
4
MONDAY,
SEPTEMBER
4
Conclusions: The augmentation of EDR at 14 and 21 days pregnancy is brought about through different mechanisms. Possible mechanisms by which EDR is augmented are the increased endothelial NO production at 14 days of pregnancy and the increased sensitivity to c-GMP at 21 days of pregnancy.
P1.15.02
ROLE OF ENDOTHELIN-1 (ET-l) IN THE HUMAN FALLOPIAN TUBE M. Sakamoto (l), S. Sakamoto (l), T. Aso (l), H. Azuma (2) (1) Dept. OBIGYN, School of Medicine, Tokyo, Japan (2) Institute of Biomaterials & Bioengineering, Tokyo Medical & Dental University, Tokyo, Japan Objectives: The present experiments were designed to investigate the role of ET-l derived from follicular fluid or tubal epithelium in the human fallopian tube. Study Methods: Fallopian tube was obtained from patients at the time of simple total hysterectomy for benign diseases with informed consent. The localization of ET-l, ET, and ET, receptor subtypes was examined immunohistochemically. Organ chamber experiments were performed to characterize the responses induced by ET-l in the longitudinal strips of ampuller segment. Results: ET-l and ET,were detectable predominantly in the tubal epithelial cells and vascular endothelial cells. ET,was detectable also in the muscle layer, but at a lesser degree. The localization of ET,was predominant in the muscle layer. Exogenously applied ET-l produced a concentration-dependent tonic contraction which was competitively antagonized by an ET, antagonist, BQ123 and an ET, / ET, antagonist, ATZ1993, but not by BQ788 as an ET, antagonist suggesting that ET, mainly mediates the tonic contraction. Rhythmic contractions and a slight increase in resting tone were produced by an addition of BQ788, suggesting the modulating role of intrinsic ET, to maintain rhythmic contraction and resting tone. Conclusions: ET-l derived from tubal epithelium and/or follicular fluid possibly plays an active role for modulating the motility of human fallopian tube through excitation of ET,and/or ET,receptor subtypes.
Pl.lS.03
FREE RADICALS AND ANTIOXlDATIVE CAPACITY DURING PREGNANCY AND DELIVERY Z. Rokvta (l), .I. Novy (l), M. Svec (l), .I. Racek (2), V. Holecek (2), L. Trefil(2) (1) Dept. OBIGYN, Charles University, Medical Faculty, Plzen, Czech Republic. (2) Inst. Clin. Biochem. Lab. Diagn., Charles University, Medical Faculty, Plzen, Czech Republic. Objectives: Free radicals play a harmful role in fertility, habitual abortions in preeclampsia, in neonatal defects. Systematic following of the markers of oxidative stress during the pregnancy as well as pregnant women exposed to increased oxidative stress like smokers, diabetics, preeclampsia are missing. The aim of the study was to investigate the changes during pregnancy and delivery in free radical action and antioxidative capacity. Study MethodsWe investigated 43 pregnant women during pregnancy and delivery on blood superoxide dimutase (SOD), glutathion peroxidase (GPx), total antioxidant capacity (AOC), glutathione (GSH) and malondialdehyde (MDA). Blood uptakes were performed 2 or 3 times during pregnancy, before and after delivery and from umbilical vein of the newborn baby. Results: SOD activity was before - after delivery - umbilical vein: 1154.9*121.5 - 1143.8*157.1 - 1193.3*114.0 U/g Hb, GPx: 51.8e14.8 - 52.Oe14.4 - 45.4e8.6 U/g Hb, AOC: 1.34eO.35 - 1.24*0.13 1.34*0.14 mmoVL (calibrated on Trolox), GSH: 1.47eO.35 - 1.40*0.35 - 1.62eO.34 mmoVL of erythrocytes, and MDA: 4.18e1.23 - 4.06eO.99 - 6.96e1.64 pmoVL. Conclusions: In the time of delivery the values of SOD and GSH are significantly lower than the activities of non-pregnant women. Lipoperoxidation (MDA) is significantly higher, probably due to postischemic reperfusion. There is a problem whether to support pregnant women by antioxidants. Extremely high values of MDA may damage some tissues especially the fetal brain.
131
Pl.lS.04
DIFFERENT DISTRIBUTION OF S-100 PROTEIN AND GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP) IMMUNOREACTIVE CELLS IN THE HUMAN FETAL SMALL INTESTINE B.A. Resch (l), E. Fekete (2) (1) Dept. OBIGYN, Szent-GyGrgyi Albert Medical University, Szeged, Hungary. (2) Dept. Zoology and Cell Biology, Jdzsef Attila University Hospital, Szeged, Hungary. Objectives: The appearance, distribution and some histochemical features of non-neuronal cells (NN ccells) associated with the myenteric plexus of human fetal small intestine have been studied by means of S100 protein and GFAl-immunocytochemistry between 10th and lTh week of gestation. In addition, double labeling immunocytochemistry using an antibody raised against a constitutive isoform of nitric oxide synthase (bNOS) in combination with an S-100 protein antibody was applied to investigate the topographic relations between NN cells and nitrergic neurons in the developing gut wall. Study Methods: Whole-mount preparations and cryostat sections of lo17 week old human fetal small intestine obtained immediately after legally approved or spontaneous abortions. Sections and whole-mount preparations were incubated overnight with anti S-100 protein and anti GFAP. The immunoreaction was visualized using 3.3’ diaminobenzidine in the presence of HzOz. Double-labeling immunofluorescence was performed applying simultaneous incubation using monoclonal mouse anti-NOS in combination with rabbit S-100 protein antiserum. Results: Cells with immunoreactivity for both glial-specific proteins appeared in the 10th week of gestation. While cells with S-100 protein immunoreactivity were located within the circular muscle layers as well as in the myenteric and submucous plexuses, cells with GFAP immunopositivity were restricted to the side of the myenteric plexus adjacent to the longitudinal muscle layer. Double labeling immunocytochemistry showed close topographical relations between nitrergic nerve fibers and the S-100 protein immunopositive cells. Conclusions: The two distant populations of NN cells appear and develop together with the enteric ganglia in the human fetal small intestine. The close proximity of S-100 protein immunopositive cells and the nitrergic fibers suggest a functional interrelationship between glial cells and nitrergic neurons during the early development of human enteric nervous system.
Pl.lS.05 MENSTRUAL CYCLE AND HORMONAL STATUS OF THE WOMEN WITH BENIGN BREAST HYPERPLASIA. I.V.Burets. A.S.Gasparov, A.I.Volobuev, P.A.Basanov,O.E.Barabanova.
Dept. REPR.,Scientific Center for Obstetrics Gynecology and Perinatology under RAMS.4 akad.Oparina Street, Moscow, Russia 117513. Objectives: The objectives of our study were to investigate menstrual cycle and hormonal status of the women with benign breast hyperplasia. Study methods: 100 women with benign breast hyperplasia were included in this study. Functional condition of the ovaries, thyroid, hypophysis were determinated on the base of physical evaluation and laboratory tasting. Results: 75% of the patients have regular menstrual cycle and 25 % patients have ovarian disfunction ( oligomenorrhea and amenorrhea). Almost 70 % of the patients have Prolactin level close to the uper limit and 30% have functional hyperprolactiemia. Thyroid dysfunction has been met in 80% of all cases. Conclusion: Women with regular menstrual cycle have a more severe pathologic changes of the breast than the women with menstrual dysfunction. Patients with benign breast hyperplasia have a various thyroid dysfunction and Prolactin level changing.
P1.14.20 ACUTE URINARY RETENTION SECONDARY TO AN IMPERFORATE HYMEN: A CASE REPORT MS. Arruda’, D.B. Tianoz. lOb/Gyn Dept., Hospital EvangClico Samaritan0 de Campinas, Campinas, SBo Paula - Brazil; 20biGyn Dept., Pontificia Universidade Catdlica de Campinas, Campinas, SBo Paula, Brazil. Objective: Report a case of acute urinary retention in an adolescent girl with hematometrocolpos causes by imperforate hymen. Study Methods: Case report Results: A healthy 12.year-old girl with a three day history of pelvic discomfort and urinary retention, had had three appointments to a pediatric emergency unit for 3 consecutive days, and diagnosed as having urinary retention. Bladder drainage by means of urethral catheter in all three visits was performed: approximately 700 ml of urine each time. On her fourth visit to the hospital, she presented again with a large volume urinary retention and important pelvic pain. She was referred to the gynecology department for evaluation. Telarche at 10 years old (Tanner II), and pubarche eight month later (Tanner II). No history of vaginal bleeding. The over distended bladder was palpable just above the umbilicus. Inspection of the vulva revealed a dome-shaped, purplishred hymenal membrane, bulging outward. On rectal examination, the vagina was palpable as a large cystic mass. The bladder was catheterized and 1500 ml of urine were drained. Ultrasound revealed a haematocolpos and haematometrium. Hymenotomy with cruciate incision was performed and 300 ml of collected blood was promptly released. Two stitches kept the central part of the hymenal membrane apart in order to avoid coalescence. Bladder catheter was removed right after the procedure and spontaneous voiding occurred five hours later. Patient was discharged in 12 hours and recovery was uneventful. Conclusions: The accumulation of the menstrual blood in the vagina and uterus may form a mechanical effect on the urethra and bladder and lead to the obstructive urinary symptoms. The impeforate hymen is a rare gynecologic abnormality that may not be detected until the onset of menses, and must be suspected in young adolescents with pelvic pain or urinary retention who has not experienced menarche.
P1.14.21 AN INVERSE RELATION BETWEEN MENARCHEAL AGE AND BODY FAT MASS AMOUNT DURING ADULTHOOD T.Douchi, M.Nakae, S.Yamamoto, Y.Nagata, Kagoshima University Hospital, 8-35-l Sakuragaoka, Kagoshima, Japan, 890-8520. Objectives: The purpose of the present study was to investigate the relation of age at menarche to adult body fat and lean mass. Study Methods: One hundred eighty-three premenopausal healthy Japanese women aged 20 to 39 years were enrolled. Baseline characteristics included age, age at menarche, and height. Body fat mass, the ratio of trunk fat mass to leg fat mass (trunk-leg fat ratio), and body lean mass were measured by dual-energy x-ray absorptiometry. Correlation of body fat or lean mass with baseline characteristics were investigated by univariate and multiple regressio analysis. Results: Age at menarche inversely correlated with adult body fat mass (r = -0.21, P < O.Ol), and tended to inversely correlate with adult body lean mass (r = -0.14, P = 0.06). However, age at menarche did not correlate with adult trunk-leg fat ratio (r = -0.11, P = 0.14). Age at menarche correlated with body fat mass, independent of age and height on multiple regression analysis (P < 0.01). Conclusions: Body fat mass amount rather than body fat distribution and body lean mass during adulthood is inversely correlated with menarcheal age, suggesting that increase in fat mass amount is a late event (i.e. onset of menarche) in the pubertal process.
MONDAY,
SEPTEMBER
P1.14.22 FREQUENCY OF MAMMARIAN DISTURBANCES IN INFANTILE AND JUVENILE POPULATION IN CUBA: THREE YEARS OF EXPERIENCE J. 0. Rodriguez, 0. R. Bermtidez, National Commission for Infantile and Juvenile Gynecology, Ministry of Public Health, Havana, Cuba. Objective: To determine the principal presenting complaints and frequency of mammary illnesses in a pediatric and adolescent urban population in Cuba. Study Methods: A prospective, longitudinal and descriptive study was carried out in 100% of the patients that assisted to infantile and juvenile gynecology consultation due to mammary problems in Havana from January 1996 to December 1998. Participant gynecologists had beforehand special training in issues related to pediatric and adolescent gynecology and they are members of the Cuban Program for the Development of Pediatric and Adolescent Reproductive Health. This program coordinated its efforts with the Cuban primary health system through its network of family doctors (coverage: 120 families/physician). This system identifies health problems by a prophylactic screening of sub-populations with risk factors, as is the infantile and adolescent population, and then refers cases to specialized consultations. Results: In total, 22,662 young women were attended at the infantile and juvenile gynecology consultations during the period of the study, an important number as self-referrals. 1103 of them required gynecologic care due to mammary disturbances (4.86%) with a higher prevalence in the adolescent years (5.29%) compared to girls younger than 10 years of age (3.87%). The most frequent diagnosis in young girls was normal puberty (39.8%), premature telarchia (39.5%) and precocious telarchia (7.9%). Common diagnoses in adolescents were normal breast development (31.3%), mastodiniainipple discharge (25.2%), fibrocystic disease (15.2%) and developmental-related complaints. Among the latter, (53.5%) and virginal hypertrophy (41.2%) were the most common. Overall, the most frequent presenting complaints were those related to puberal developmental disorders and with normal mammary gland growth. Conclusions: The most frequent presenting complaints in our population were those related to puberal developmental disorders and with normal mammary gland growth. A plausible explanation for this is the relatively high frequency of self-referral cases that existed after the system begun its function.
P1.15
PHYSIOLOGY
OF REPRODUCTION
1
Pl.lS.01 GESTATIONAL CHANGES IN ENDOTHELIUM-DEPENDENT RELAXATION (EDR) OF RAT AORTA S. Sakamoto (l), A. Wakabayashi (l), M. Harada (l), A. Nagai (l), T. Aso( M. Goto (2), H. Azuma (2) (1) Dept. OBIGYN, Tokyo Medical and Dental University, Tokyo, Japan (2) Inst. Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan Objective: The present experiments were designed to investigate the gestational changes in endothelium-dependent relaxation (EDR) of rat aorta. Study Methods: Thoracic aortas were taken from non-pregnant 7, 14 and 21 days pregnant SD rats and 2mm width transverse strips with endothelium [E (+)] or without endothelium [E (-)] were made. After tonic contraction was induced by 10 -‘M U46619, EDR induced by acetylcholine (ACh) (109-3x10~5 M) in E (+) strips and relaxation in response to sodium nitroprusside (SNP) (109-3x10~5 M) in E (-) strips were observed. Results: In E (+) strips, ACh-induced EDR was unchanged at 7 days pregnancy and augmented at 14 and 21 days pregnancy when comparison was made on the non-pregnancy. In E (-) strips, SNPinduced relaxation was unchanged at 7 and 14 days pregnancy and augmented at 21 days pregnancy when compared with that at nonpregnancy. Basal and ACh-stimulated c-GMP levels in aorta were augmented at 14 days pregnancy and unchanged at 21 days pregnancy when compared with those at non-pregnancy.
4
MONDAY,
SEPTEMBER
4
Conclusions: The augmentation of EDR at 14 and 21 days pregnancy is brought about through different mechanisms. Possible mechanisms by which EDR is augmented are the increased endothelial NO production at 14 days of pregnancy and the increased sensitivity to c-GMP at 21 days of pregnancy.
P1.15.02
ROLE OF ENDOTHELIN-1 (ET-l) IN THE HUMAN FALLOPIAN TUBE M. Sakamoto (l), S. Sakamoto (l), T. Aso (l), H. Azuma (2) (1) Dept. OBIGYN, School of Medicine, Tokyo, Japan (2) Institute of Biomaterials & Bioengineering, Tokyo Medical & Dental University, Tokyo, Japan Objectives: The present experiments were designed to investigate the role of ET-l derived from follicular fluid or tubal epithelium in the human fallopian tube. Study Methods: Fallopian tube was obtained from patients at the time of simple total hysterectomy for benign diseases with informed consent. The localization of ET-l, ET, and ET, receptor subtypes was examined immunohistochemically. Organ chamber experiments were performed to characterize the responses induced by ET-l in the longitudinal strips of ampuller segment. Results: ET-l and ET,were detectable predominantly in the tubal epithelial cells and vascular endothelial cells. ET,was detectable also in the muscle layer, but at a lesser degree. The localization of ET,was predominant in the muscle layer. Exogenously applied ET-l produced a concentration-dependent tonic contraction which was competitively antagonized by an ET, antagonist, BQ123 and an ET, / ET, antagonist, ATZ1993, but not by BQ788 as an ET, antagonist suggesting that ET, mainly mediates the tonic contraction. Rhythmic contractions and a slight increase in resting tone were produced by an addition of BQ788, suggesting the modulating role of intrinsic ET, to maintain rhythmic contraction and resting tone. Conclusions: ET-l derived from tubal epithelium and/or follicular fluid possibly plays an active role for modulating the motility of human fallopian tube through excitation of ET,and/or ET,receptor subtypes.
Pl.lS.03
FREE RADICALS AND ANTIOXlDATIVE CAPACITY DURING PREGNANCY AND DELIVERY Z. Rokvta (l), .I. Novy (l), M. Svec (l), .I. Racek (2), V. Holecek (2), L. Trefil(2) (1) Dept. OBIGYN, Charles University, Medical Faculty, Plzen, Czech Republic. (2) Inst. Clin. Biochem. Lab. Diagn., Charles University, Medical Faculty, Plzen, Czech Republic. Objectives: Free radicals play a harmful role in fertility, habitual abortions in preeclampsia, in neonatal defects. Systematic following of the markers of oxidative stress during the pregnancy as well as pregnant women exposed to increased oxidative stress like smokers, diabetics, preeclampsia are missing. The aim of the study was to investigate the changes during pregnancy and delivery in free radical action and antioxidative capacity. Study MethodsWe investigated 43 pregnant women during pregnancy and delivery on blood superoxide dimutase (SOD), glutathion peroxidase (GPx), total antioxidant capacity (AOC), glutathione (GSH) and malondialdehyde (MDA). Blood uptakes were performed 2 or 3 times during pregnancy, before and after delivery and from umbilical vein of the newborn baby. Results: SOD activity was before - after delivery - umbilical vein: 1154.9*121.5 - 1143.8*157.1 - 1193.3*114.0 U/g Hb, GPx: 51.8e14.8 - 52.Oe14.4 - 45.4e8.6 U/g Hb, AOC: 1.34eO.35 - 1.24*0.13 1.34*0.14 mmoVL (calibrated on Trolox), GSH: 1.47eO.35 - 1.40*0.35 - 1.62eO.34 mmoVL of erythrocytes, and MDA: 4.18e1.23 - 4.06eO.99 - 6.96e1.64 pmoVL. Conclusions: In the time of delivery the values of SOD and GSH are significantly lower than the activities of non-pregnant women. Lipoperoxidation (MDA) is significantly higher, probably due to postischemic reperfusion. There is a problem whether to support pregnant women by antioxidants. Extremely high values of MDA may damage some tissues especially the fetal brain.
131
Pl.lS.04
DIFFERENT DISTRIBUTION OF S-100 PROTEIN AND GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP) IMMUNOREACTIVE CELLS IN THE HUMAN FETAL SMALL INTESTINE B.A. Resch (l), E. Fekete (2) (1) Dept. OBIGYN, Szent-GyGrgyi Albert Medical University, Szeged, Hungary. (2) Dept. Zoology and Cell Biology, Jdzsef Attila University Hospital, Szeged, Hungary. Objectives: The appearance, distribution and some histochemical features of non-neuronal cells (NN ccells) associated with the myenteric plexus of human fetal small intestine have been studied by means of S100 protein and GFAl-immunocytochemistry between 10th and lTh week of gestation. In addition, double labeling immunocytochemistry using an antibody raised against a constitutive isoform of nitric oxide synthase (bNOS) in combination with an S-100 protein antibody was applied to investigate the topographic relations between NN cells and nitrergic neurons in the developing gut wall. Study Methods: Whole-mount preparations and cryostat sections of lo17 week old human fetal small intestine obtained immediately after legally approved or spontaneous abortions. Sections and whole-mount preparations were incubated overnight with anti S-100 protein and anti GFAP. The immunoreaction was visualized using 3.3’ diaminobenzidine in the presence of HzOz. Double-labeling immunofluorescence was performed applying simultaneous incubation using monoclonal mouse anti-NOS in combination with rabbit S-100 protein antiserum. Results: Cells with immunoreactivity for both glial-specific proteins appeared in the 10th week of gestation. While cells with S-100 protein immunoreactivity were located within the circular muscle layers as well as in the myenteric and submucous plexuses, cells with GFAP immunopositivity were restricted to the side of the myenteric plexus adjacent to the longitudinal muscle layer. Double labeling immunocytochemistry showed close topographical relations between nitrergic nerve fibers and the S-100 protein immunopositive cells. Conclusions: The two distant populations of NN cells appear and develop together with the enteric ganglia in the human fetal small intestine. The close proximity of S-100 protein immunopositive cells and the nitrergic fibers suggest a functional interrelationship between glial cells and nitrergic neurons during the early development of human enteric nervous system.
Pl.lS.05 MENSTRUAL CYCLE AND HORMONAL STATUS OF THE WOMEN WITH BENIGN BREAST HYPERPLASIA. I.V.Burets. A.S.Gasparov, A.I.Volobuev, P.A.Basanov,O.E.Barabanova.
Dept. REPR.,Scientific Center for Obstetrics Gynecology and Perinatology under RAMS.4 akad.Oparina Street, Moscow, Russia 117513. Objectives: The objectives of our study were to investigate menstrual cycle and hormonal status of the women with benign breast hyperplasia. Study methods: 100 women with benign breast hyperplasia were included in this study. Functional condition of the ovaries, thyroid, hypophysis were determinated on the base of physical evaluation and laboratory tasting. Results: 75% of the patients have regular menstrual cycle and 25 % patients have ovarian disfunction ( oligomenorrhea and amenorrhea). Almost 70 % of the patients have Prolactin level close to the uper limit and 30% have functional hyperprolactiemia. Thyroid dysfunction has been met in 80% of all cases. Conclusion: Women with regular menstrual cycle have a more severe pathologic changes of the breast than the women with menstrual dysfunction. Patients with benign breast hyperplasia have a various thyroid dysfunction and Prolactin level changing.