Glyoxalase-I Activity and Cell Cycle Regulatiomn in Yeast

Vol. 121, No. 1, 1984 May 31, 1984

BIOCHEMICAL

AND BIOPHYSICAL RESEARCH COMMUNICATIONS Page

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article

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"Glyoxalase-I

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Kumar Dudani,

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Activity

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Table

Glyoxalase-I activity cell division cycle

1

in normally growing and G arrested mutants of Saccharomyces c Qrevisiae

S.cerevisiae Mutants

Activity (~moles/min/mg Normal

protein) Arrested

cdc 28

0.69

0.31

55

cdc

4

0.87

0.36

58

cdc

7

0.96

0.63

35

24

3.66

0.90

75

cdc

$Inhibition

Cells were synchronized as described in Materials and Methods. In all cases, 90-95% synchrony was obtained. Glyoxalase-I activity was determined as described in Materials and Methods. All values are an average of 3-4 independent determinations.

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0 1984 by Academic Press, Inc. of reproduction in any form reserved.

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