Gonadotrophic hormone secretion from hydatid mole grown in tissue culture

There the pituitary,

were no untoward side effects. this is by no means considered

Vol.

$2, Just:

While it provrtl.

appears

that

there

\VAI.TER 66, No,

“Froewis,

intluen(~~~

F. T~UBER,

lin

XI).

4, 1954.

J., Rrrmer, ll., and Narik, Interposition Operation, p. 391.

Froewis:

is some

Indications

(1.:

lntlivatiww

for and Results of Watkins

for

Results

:lntl

Interposition

of the

\2’:rtkins’

Operation,

p. 39 I.

The authors consider the Watkins interposition operation as Irest for cystoccle, proIn their hands, the following results were of the uterus, and stress incontinence. obtained: 75 per cent cure and 20 per cent improvement of incontinence and 70 per cent taure and 15 per cent improvement of various types of prolapse. This compares favorabl? They advise t.lmt with the results obtained with other operations for these conditions. great care be taken in positioning the uterus properly and that small or atrophic uteri 1)~ Good posterior eolporrhaphy is also recluire
r-01. 66, No. 31, “hg. 6, 1954. Brandl, I(., and Pi&a, l-01.

66, No.

33, Bun.

.sRurkl,

W. :

20,

The

1:. :

Examination

and

Treatment

of

Vaginitis,

p. 546.

1954.

Interstitial

Cells

of t,he Gonads

as the

Site

of

Srs

Ilorrrronc

Proclw*ti~rn,

p. 575. Burkl : Interstitial It stromal

Cells of Gonads as Site of Sex Hormone

is demonstrated cells in the ovary

by a new histochemical produce t,estosterone

method that Leydig cells and estrogen, respectively. WALTER

7’01. 66, No. 34, Aug. $7, 1954. Hofstaetter, R. : The Usefulness Uterine Cancer, p. 590.

Journal 701.

14, No. *Waltz,

Walte,

5, April,

Production,

of Wessely’s

of the National

“Relascope”

in

in

p. 575. the

I?. TAIJBER,

t,he Early

testis

and

M.D.

Diagnosis

of

Cancer Institute

1954.

Helen K., Wilton R.: Tissue Culture,

Tullner, William W., Evans, Virginia J., Hertz, Gonadotrophic Hormone Secretion From Hydatid p. 1173.

Tullner, Evans, Hertz, and Earle: Gonadotrophic Hydatid Mole Grown in Tissue Uulture, p. 1173.

Hotione

Roy, Mole

and Earle, Grown in

Secretion

From

The authors obtained fresh surgical specimens from four cases of hydatid mole. The vesicles were cut into small sections. Tissue pieces of comparable weight from the same bleb were paired; one piece was frozen for assay and the other was planted. The 20 per cent chick-embryo extract, 40 per cent horse serum, culture fluid was composed of: and 40 per cent Earle’s saline containing penicillin and streptomycin. A modified LevinTyndale test was employed in doing the biological assays. The studies revealed that the heterologous cultures produced gonadotrophic hormone over an extended period of time-as long as 413 days in one case. In addition, increases in gonadotrophic activity from 600 to 15,000 per cent were demonstrated. Not only were the authors unable to predict the gonadotrophic activity of the mole from the potency of the original tissue homogenate, but, also, study of cell types in the living culture did not indicate any particular cell type as the source of the gonadotrophic hormone. JOHN

G. MASTERSON,

M.D.