- Email: [email protected]
Green Rooibos Extract improves plasma lipid profile and oxidative status in diabetic non-human primates
D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107 6
Department of Food Science, Stellenbosch University, Matieland, 7602, South Africa
Rooibos,a shrub-like leguminous bush endemic to South Africa, is traditionally used as a herbal tea. Unfermented (green) rooibos presents with a much higher polyphenol content, in particular aspalathin which constitutes a major metabolically bioactive compound. Recent studies, both in vitro and ex vivo, highlighted an enhanced glucose uptake effect of aspalathin, associated with increased GLUT 4 translocation to the plasma membrane via AMPK activation. In high fat fed mice, aspalathin also showed a plasma lipid lowering effect. In the present study, we evaluated the effect of 4 weeks of supplementation (90 mg/kg body weight t.i.d) with a pharmaceutical grade aspalathin-enriched green rooibos extract containing ca. 12.8% aspalathin, on gluco-lipidic and oxidative indexes in high fat diet-induceddiabetic and normal non-human primates (Chlorocebus aethiops). Diabetic models were characterized by glucose intolerance and increased total cholesterol and LDL. Moreover, decreased plasma CoQ10 and increased oxidative status were associated with higher levels of oxLDL. Green rooibos supplementation of the diabetic animals significantly improved all listed indices, highlighting the potentialities of this natural extract in modulating some risk factors associated with cardiovascular disease associated with type 2 diabetes. E-mail address: [email protected] (P. Orlando) Acknowledgements
Italy-South Africa Strategic Scientific Cooperation Ministry of Foreign Affairs http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.313
P-229
Ubiquinol supplemenetation in elderly patients undergoing aortic valve replacement: Biochemical and clinical effects Patrick Orlando 1, Francesco Nicolini 2, Tiziano Gherli 2, Alberto Molardi 2, Sonia Silvestri 1, Francesca Brugè 1, Gian Paolo Littarru 3, Luca Tiano 1 1
Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy 2 Cardiac Surgery Unit, Unversity of Parma, Italy 3 Department of Clinical and Dental Sciences, Polytechnic University of Marche, Italy
There is a steady rise in the mean age of patients affected by heart disease undergoing cardiac surgery. However senescent myocardium has reduced tolerance to ischemic stress and there are clear indications about age-associated deficit in myocardial performance after operatory stress. CoQ10 improve several conditions related to bioenergetic deficit or increased exposure to oxidative stress. The use of ubiquinol, the reduced form of CoQ10, is promising in these clinical settings, on the basis of its superior bioavailability and of the alleged impaired CoQ10-reducing capacity in the elderly. In this study clinical and biochemical effects of ubiquinol were evaluated in 46 patients undergoing aortic valve replacement. They, affected by severe aortic stenosis, were randomized into 2 groups, placebo and CoQ10 group supplemented
S97
with 400mg/day of ubiquinol starting, 7 days before surgery and continuing for 1 month after cardiac intervention. CoQ10 levels, its oxidative status and IL-6, TNF-alpha and S100 protein concentration in plasma were assessed. Moreover, main cardiac adverse effects in the postoperatory phase, NYHA class, contractility and myocardial hypertrophy were evaluated. Ubiquinol treatment improve basal (before surgery) oxidative status of CoQ10 and considerably mitigate increased oxidation related to the operation. E-mail address: [email protected] (P. Orlando) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.314
P-230
Simvastatin differently effect oxidative status in cultured myocytes from young and old donors Sonia Silvestri 1, Gilliant Butler Browne 2, Louiza Arouche 2, Patrick Orlando 1, Fabio Marcheggiani 3, Ilenia Cirilli 3, Francesca Brugè 3, Michele Guescini 4, Luca Tiano 1 1
Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy 2 Center of Research in Myology UMRS974 - UPMC Sorbonne Universités 3 Department of Biomolecular Sciences - University of Urbino 4 Department of Clinical Dental Sciences, Polytechnic University of Marche, Italy
Sarcopenia is a condition of age-related loss of muscle mass and strength which affects balance, gait and overall ability to perform tasks of daily living, with increased risk of falls. While mitochondrial dysfunction and associated oxidative stress contribute to muscle ageing, the interplay between ROS and protective antioxidant response in the cell is regarded as a key factor in maintaining muscle strength and physical performance. CoQ10 is a lypophilic endogenous cofactor with both antioxidant and bioenergetics function whose levels decrease with age. Moreover also lipid-lowering drugs targeting HMG-CoA reductase (statin) are able to effect CoQ10 synthesis. In the present study we show that murine myocytes (C2C12) exposed to simvastatin 0.3-40 μM for 72 h show a reduced content of CoQ associated with increased ROS production and cytotoxicity. Moreover exposure in the same conditions of primary human myocytes from young and old donors show an enhanced susceptibility to oxidative stress in the elderly a condition that might suggest an enhanced detrimental side effects of statins with potential implications in sarcopenia development. E-mail address: [email protected] (S. Silvestri)
Acknowledgements
Embassy of France in Italy mobility grant http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.315
Department of Food Science, Stellenbosch University, Matieland, 7602, South Africa
Rooibos,a shrub-like leguminous bush endemic to South Africa, is traditionally used as a herbal tea. Unfermented (green) rooibos presents with a much higher polyphenol content, in particular aspalathin which constitutes a major metabolically bioactive compound. Recent studies, both in vitro and ex vivo, highlighted an enhanced glucose uptake effect of aspalathin, associated with increased GLUT 4 translocation to the plasma membrane via AMPK activation. In high fat fed mice, aspalathin also showed a plasma lipid lowering effect. In the present study, we evaluated the effect of 4 weeks of supplementation (90 mg/kg body weight t.i.d) with a pharmaceutical grade aspalathin-enriched green rooibos extract containing ca. 12.8% aspalathin, on gluco-lipidic and oxidative indexes in high fat diet-induceddiabetic and normal non-human primates (Chlorocebus aethiops). Diabetic models were characterized by glucose intolerance and increased total cholesterol and LDL. Moreover, decreased plasma CoQ10 and increased oxidative status were associated with higher levels of oxLDL. Green rooibos supplementation of the diabetic animals significantly improved all listed indices, highlighting the potentialities of this natural extract in modulating some risk factors associated with cardiovascular disease associated with type 2 diabetes. E-mail address: [email protected] (P. Orlando) Acknowledgements
Italy-South Africa Strategic Scientific Cooperation Ministry of Foreign Affairs http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.313
P-229
Ubiquinol supplemenetation in elderly patients undergoing aortic valve replacement: Biochemical and clinical effects Patrick Orlando 1, Francesco Nicolini 2, Tiziano Gherli 2, Alberto Molardi 2, Sonia Silvestri 1, Francesca Brugè 1, Gian Paolo Littarru 3, Luca Tiano 1 1
Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy 2 Cardiac Surgery Unit, Unversity of Parma, Italy 3 Department of Clinical and Dental Sciences, Polytechnic University of Marche, Italy
There is a steady rise in the mean age of patients affected by heart disease undergoing cardiac surgery. However senescent myocardium has reduced tolerance to ischemic stress and there are clear indications about age-associated deficit in myocardial performance after operatory stress. CoQ10 improve several conditions related to bioenergetic deficit or increased exposure to oxidative stress. The use of ubiquinol, the reduced form of CoQ10, is promising in these clinical settings, on the basis of its superior bioavailability and of the alleged impaired CoQ10-reducing capacity in the elderly. In this study clinical and biochemical effects of ubiquinol were evaluated in 46 patients undergoing aortic valve replacement. They, affected by severe aortic stenosis, were randomized into 2 groups, placebo and CoQ10 group supplemented
S97
with 400mg/day of ubiquinol starting, 7 days before surgery and continuing for 1 month after cardiac intervention. CoQ10 levels, its oxidative status and IL-6, TNF-alpha and S100 protein concentration in plasma were assessed. Moreover, main cardiac adverse effects in the postoperatory phase, NYHA class, contractility and myocardial hypertrophy were evaluated. Ubiquinol treatment improve basal (before surgery) oxidative status of CoQ10 and considerably mitigate increased oxidation related to the operation. E-mail address: [email protected] (P. Orlando) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.314
P-230
Simvastatin differently effect oxidative status in cultured myocytes from young and old donors Sonia Silvestri 1, Gilliant Butler Browne 2, Louiza Arouche 2, Patrick Orlando 1, Fabio Marcheggiani 3, Ilenia Cirilli 3, Francesca Brugè 3, Michele Guescini 4, Luca Tiano 1 1
Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy 2 Center of Research in Myology UMRS974 - UPMC Sorbonne Universités 3 Department of Biomolecular Sciences - University of Urbino 4 Department of Clinical Dental Sciences, Polytechnic University of Marche, Italy
Sarcopenia is a condition of age-related loss of muscle mass and strength which affects balance, gait and overall ability to perform tasks of daily living, with increased risk of falls. While mitochondrial dysfunction and associated oxidative stress contribute to muscle ageing, the interplay between ROS and protective antioxidant response in the cell is regarded as a key factor in maintaining muscle strength and physical performance. CoQ10 is a lypophilic endogenous cofactor with both antioxidant and bioenergetics function whose levels decrease with age. Moreover also lipid-lowering drugs targeting HMG-CoA reductase (statin) are able to effect CoQ10 synthesis. In the present study we show that murine myocytes (C2C12) exposed to simvastatin 0.3-40 μM for 72 h show a reduced content of CoQ associated with increased ROS production and cytotoxicity. Moreover exposure in the same conditions of primary human myocytes from young and old donors show an enhanced susceptibility to oxidative stress in the elderly a condition that might suggest an enhanced detrimental side effects of statins with potential implications in sarcopenia development. E-mail address: [email protected] (S. Silvestri)
Acknowledgements
Embassy of France in Italy mobility grant http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.315