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Gross Cytopathology: Are Macroscopic Images of Value in Cytology?
S64
Abstracts
Table 1.
HPV Positivity in Different modified-LBC
143 Rapid Removal of Cytology Slide Coverslips for DNA and RNA Isolation Barbara Chadwick, MD, Wenhua Zhou, CT(ASCP), PhD, Katherine Geiersbach, MD University of Utah/ARUP Institute, Salt Lake City, Utah Introduction: As molecular targets for diagnosis and treatment increase, more ancillary tests being developed are requiring the use of cytology specimens. One time-consuming step in this procedure is glass coverslip removal by xylene. Here we describe a validation procedure and method for rapid coverslip removal using liquid nitrogen. Materials and Methods: Direct smears were prepared from residual pleural fluid, Diff-Quik stained, then covered with a glass coverslip. Coverslips were then removed by either immersing the slides in xylene for 3 to 4 days, or by razor blade after rapidly freezing the slides in liquid nitrogen for 30 seconds. The DNA or RNA was extracted by Pinpoint Slide Isolation system (Zymo Research Corp) or RNeasy FFPE kit (Qiagen). The quantity of DNA and RNA from the two different procedures was evaluated by Quib DNA or RNA kit, respectively. The amplifiability of the DNA and RNA was also evaluated by real-time PCR and RT-PCR with housekeeping gene primers. Results: The concentration of DNA isolated using the two procedures was not significantly different (2.79 ug/ml for liquid nitrogen method vs 2.56 ug/ml for Xylene method, nZ5). Similar results were observed for RNA concentration (13.28 ug/mL for liquid nitrogen vs 12.22 ug/ml for Xylene, nZ5). The real time PCR results indicate that the DNA extracted from the two procedures can be amplified without significant difference (Ct: 22.41 for liquid nitrogen method vs 22.38 for xylene method, nZ5). Similar results were obtained from real time RT-PCR for RNA samples (Ct: 30.75 for liquid nitrogen method vs 30.97 for xylene method, nZ5). Conclusions: Liquid nitrogen coverslip removal allows for DNA and RNA extraction from direct smear slides that is equivalent to the xylene coverslip removal method, without affecting the quantity and quality of the nucleic acids, and allows for expedited processing.
institution, we began capturing macroscopic images related to cytology cases. The aim of this study was to evaluate the utility of acquiring such images. Materials and Methods: Handheld digital cameras were used to take photos. Captured digital images (jpeg format) were uploaded to our laboratory information system (LIS) using an integrated image management module (PICSPlus, CoPath, Cerner). A retrospective review was performed (42 month period) for all cytology cases with associated macroscopic images. Results: 59 cases were identified in which 133 images were uploaded. There were on average 2 images per case (range 1 to 7 images/case). Most (53 cases, 90%) images were taken in the FNA clinic and the rest in radiology (5 cases) or gastrointestinal endoscopy suite (1 case). Images were of physical lesions (figure A) that were aspirated in 45 cases (76%) and samples (figure B) in 9 cases (30%). Images in some cases documented the FNA site marked by the clinician (figure C). Cytopathologists were more likely to photograph lesions on the extremities (21 cases) and trunk (20 cases) than the breast (4 cases), neck (3 cases) or face (1 case). With the recent Introduction of pathologist-performed ultrasound guided FNA in our clinic, ultrasound images (figure D) were uploaded in 5 cases into our LIS to document needle guidance and sonographic attributes of targeted lesions. Conclusion: Macroscopic images can be of value in cytopathology. This includes gross pathology images of specimens, photos of physical lesions to be aspirated, and ultrasound images. Such images help convey important clinical information, are useful for education and research purposes, and serve as a mechanism for critical procedural documentation.
Figures A-D
Macroscopic cytology images
145 144 Gross Cytopathology: Are Macroscopic Images of Value in Cytology? Liron Pantanowitz, MD, Jacqueline Cuda, BS, SCT(ASCP), Juan Xing, MD, Anil Parwani, MD, PhD, Sara Monaco, MD University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania Introduction: Gross pathology images are routinely obtained in surgical pathology for diagnostic and documentation purposes. Such macroscopic images are rarely taken in the practice of cytopathology. At our
Three Dimensional Cytologic Examination: Performance of the Pathologist Reviewer in the LuCEDÒ Test for Lung Cancer Screening David Wilbur, MD1, Jennifer Eschbacher, MD2, Gene Pawlick, MD, (retired)3 1 Massachusetts General Hospital, Boston, Massachusetts; 2 St. Joseph Hospital and Medical Center; 3 Kaiser Permanente, Berkeley, California Introduction: The LuCED test (VisionGate, Phoenix, AZ) for detection of lung cancer is based on 3 dimensional digital cell reconstructions (Cell
Abstracts
Table 1.
HPV Positivity in Different modified-LBC
143 Rapid Removal of Cytology Slide Coverslips for DNA and RNA Isolation Barbara Chadwick, MD, Wenhua Zhou, CT(ASCP), PhD, Katherine Geiersbach, MD University of Utah/ARUP Institute, Salt Lake City, Utah Introduction: As molecular targets for diagnosis and treatment increase, more ancillary tests being developed are requiring the use of cytology specimens. One time-consuming step in this procedure is glass coverslip removal by xylene. Here we describe a validation procedure and method for rapid coverslip removal using liquid nitrogen. Materials and Methods: Direct smears were prepared from residual pleural fluid, Diff-Quik stained, then covered with a glass coverslip. Coverslips were then removed by either immersing the slides in xylene for 3 to 4 days, or by razor blade after rapidly freezing the slides in liquid nitrogen for 30 seconds. The DNA or RNA was extracted by Pinpoint Slide Isolation system (Zymo Research Corp) or RNeasy FFPE kit (Qiagen). The quantity of DNA and RNA from the two different procedures was evaluated by Quib DNA or RNA kit, respectively. The amplifiability of the DNA and RNA was also evaluated by real-time PCR and RT-PCR with housekeeping gene primers. Results: The concentration of DNA isolated using the two procedures was not significantly different (2.79 ug/ml for liquid nitrogen method vs 2.56 ug/ml for Xylene method, nZ5). Similar results were observed for RNA concentration (13.28 ug/mL for liquid nitrogen vs 12.22 ug/ml for Xylene, nZ5). The real time PCR results indicate that the DNA extracted from the two procedures can be amplified without significant difference (Ct: 22.41 for liquid nitrogen method vs 22.38 for xylene method, nZ5). Similar results were obtained from real time RT-PCR for RNA samples (Ct: 30.75 for liquid nitrogen method vs 30.97 for xylene method, nZ5). Conclusions: Liquid nitrogen coverslip removal allows for DNA and RNA extraction from direct smear slides that is equivalent to the xylene coverslip removal method, without affecting the quantity and quality of the nucleic acids, and allows for expedited processing.
institution, we began capturing macroscopic images related to cytology cases. The aim of this study was to evaluate the utility of acquiring such images. Materials and Methods: Handheld digital cameras were used to take photos. Captured digital images (jpeg format) were uploaded to our laboratory information system (LIS) using an integrated image management module (PICSPlus, CoPath, Cerner). A retrospective review was performed (42 month period) for all cytology cases with associated macroscopic images. Results: 59 cases were identified in which 133 images were uploaded. There were on average 2 images per case (range 1 to 7 images/case). Most (53 cases, 90%) images were taken in the FNA clinic and the rest in radiology (5 cases) or gastrointestinal endoscopy suite (1 case). Images were of physical lesions (figure A) that were aspirated in 45 cases (76%) and samples (figure B) in 9 cases (30%). Images in some cases documented the FNA site marked by the clinician (figure C). Cytopathologists were more likely to photograph lesions on the extremities (21 cases) and trunk (20 cases) than the breast (4 cases), neck (3 cases) or face (1 case). With the recent Introduction of pathologist-performed ultrasound guided FNA in our clinic, ultrasound images (figure D) were uploaded in 5 cases into our LIS to document needle guidance and sonographic attributes of targeted lesions. Conclusion: Macroscopic images can be of value in cytopathology. This includes gross pathology images of specimens, photos of physical lesions to be aspirated, and ultrasound images. Such images help convey important clinical information, are useful for education and research purposes, and serve as a mechanism for critical procedural documentation.
Figures A-D
Macroscopic cytology images
145 144 Gross Cytopathology: Are Macroscopic Images of Value in Cytology? Liron Pantanowitz, MD, Jacqueline Cuda, BS, SCT(ASCP), Juan Xing, MD, Anil Parwani, MD, PhD, Sara Monaco, MD University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania Introduction: Gross pathology images are routinely obtained in surgical pathology for diagnostic and documentation purposes. Such macroscopic images are rarely taken in the practice of cytopathology. At our
Three Dimensional Cytologic Examination: Performance of the Pathologist Reviewer in the LuCEDÒ Test for Lung Cancer Screening David Wilbur, MD1, Jennifer Eschbacher, MD2, Gene Pawlick, MD, (retired)3 1 Massachusetts General Hospital, Boston, Massachusetts; 2 St. Joseph Hospital and Medical Center; 3 Kaiser Permanente, Berkeley, California Introduction: The LuCED test (VisionGate, Phoenix, AZ) for detection of lung cancer is based on 3 dimensional digital cell reconstructions (Cell