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GW27-e0562 The value of high sensitive troponin-i in early diagnosis Of acute myocardial infarction
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, VOL. 68, NO. 16, SUPPL S, 2016
C179
expression of cytokines of myofibroblasts, finally transfer the control lentivirus and experimental lentivirus into cells, then evaluated the expression and influence of C/EBPb to myocardial fibrosis, assess the proportion of different subtypes of C/EBPb(LAP, LAP* and LIP) and the ratio between them, assess the degree of fibrosis in each group, detected the expression of downstream proteins cofilin and FLNa, and investigate the relationship of C/EBPb and cofilin, FLNa. In vivo, 24 Lewis rats were randomly divided into the control group, the myosin group, myosinþ control lentivirus group and the myosinþ experimental lentivirus group. The control group did not receive any disposal, other groups were given footpad injection of porcine cardiac myosin 1mg/per rat twice, on the first day and the seventh day respectively. Then import the control lentivirus and experimental lentivirus fused ultrasound microbubble via femoral vein injection combined with local ultrasonic irradiation. The ultrasound indicators of heart were observed, mainly include IVS, IVPW, LVEDD, LVESD, EF, FS and so on. The degree of inflammation in the myocardium was quantified by HE staining, and cardiac fibrosis was quantified by Masson staining. We also measured the expression of a-SMA, C/EBPb, MMP2, MMP9, collagen I, collagen III by immunohistochemistry, the expression of C/EBPb in each group by western blot.
to iohexol group SIRTl protein expression in each group were significantly lower, FOXO3a protein increased, the differences were statistically significant (bP <0.05). IohexolþNA group than the control group, SIRT1, FOXO3a protein expression decreased significantly (aP <0.05); iohexol þ FOXO3a siRNA group than control group, the expression of SIRT1 protein had no significant change (P>0.05), FOXO3a protein decreased expression (aP<0.05), the differences were statistically significant.
RESULTS When C/EBPb was silenced, the myocardial fibrosis, the ratio between LAP and LIP was increased, at the same time the expression of downstream protein cofilin and FLNa were reduced. Compared with the control group, the experimental group has a lower inflammation and fibrosis (P<0.01) level. The expression of a-SMA, C/ EBPb, MMP2, MMP9, collagen I, collagen III were reduced simultaneously in the experimental groups (P<0.05).
OBJECTIVES To observe the effect of curcumin (CUR) intervention on contrast-induced acute kidney injury in experimental rats, and to explore the beneficial effects and the possible mechanism of curcumin intervention on acute kidney injury in experimental rats by the low non-ionic contrast medium, in order to provide more theoretical basis for treating such injury in clinical practice.
CONCLUSIONS Inhibition of the expression of transcription factor C/ EBPb could reduce myocardial fibrosis and inflammation. Inhibition the expression of cofilin and FLNa might be the underlying mechanism. GW27-e0410 Effect of astaxanthin intervention on contrast induced acute kidney injury through the SIRT1/FOXO3a signal Li Wenhua Department of Cardiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou (221002), Jiangsu, China OBJECTIVES To observe the cell proliferation ability, ROS, expressions of SIRT1, FOXO3a in AKI model induced by iodine in HK-2 cells and the effects of astaxanthin on it and its possible mechanisms will be also investigated. Our study will provide a theoretical evidence of astaxanthin on the prevention and treatment of contrast induced acute kidney injury. METHODS HK-2 cells were cultured in DMEM/F12 medium in vitro and then randomly divided into appropriate experimental groups: normal group, dimethyl sulfoxide (DMSO), iohexol group, iohexol þ (1.0, 10.0 mmol/L) astaxanthin group, iohexol þ SIRT1 inhibitors (Nicotinamide, NA) group and iohexol þ siRNA FOXO3a group;when were cultured at 24 hours, cell proliferation ability was tested by CCK-8 method, ROS were detected by flow cytometry, The expression of SIRT1 and FOXO3a were observed using western-blot. RESULTS (1) CCK-8 Results: ① with the increase of concentration of astaxanthin HK-2 cells proliferation has not changed, when AST concentrations > 15mmol/L, cell proliferation was significantly reduced,(aP<0.05), the difference was statistically significant. ② when concentration of the iohexol > 200gI/L, the cell proliferation decreased,(aP<0.05), the difference was statistically significant.③nicotinamide concentration> 20mmol/L, the cell proliferation decreased, (aP <0.05), the difference was statistically significant. (2) Flow cytometry results showed that (after intervention 24 h): compared to the negative control group, the level of ROS in the iohexol group, iohexol þ 1.0mmol/L, 10mmol/L astaxanthin group was significantly increased, the differences were statistically significant (aP<0.05); and between iohexol group and iohexolþ (1.0,10.0mmol/L) astaxanthin group, iohexolþ1.0m mol/L astaxanthin group and iohexol þ 10.0mmol/L astaxanthin group, differences were statistically significant (bP <0.05). (3) Western blot results are shown, iohexol group than normal group SIRTl protein were significantly increased, FOXO3a protein expression decreased, the differences were statistically significant (aP <0.05); iohexolþ(1.0,10.0 mmol/L) astaxanthin group compared
CONCLUSIONS astaxanthin inhibits iohexol induced damage in HK-2, and its possible mechanism is related to decrease expression of endothelial cells SIRT1, and on the upstream of FOXO3a plays against damage induced by iohexol.
GW27-e0413 Effect of Curcumin Intervention on Contrast-induced Kidney Injury in Experimental Rats through Upregulating the Expression of Sirt1 Li Wenhua Department of Cardiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou (221002), Jiangsu, China
METHODS A total of 30 male SD rats were randomly divided into 3 groups: control group, contrast-induced nephropathy group and curcumin group, ten of each group. The rats of curcumin group received intra-gastric curcumin for 5 days, the other two groups were given the corresponding volume of solvent for 5 days. After 48 hours of building model, The renal function was evaluated by serum creatinine(Scr) and urea nitrogen(BUN), the pathological change of the renal tissue was observed by HE staining to evaluate the degree of renal tissue injury, the nephridial oxidative stress indexes of malonadehyde (MDA) level and super oxide dismutase (SOD) activity were measured by the operating kits, then the protein expression of Sirt1 and NF-kB was examined by westernblot. RESULTS Compared with control group, contrast-induced nephropathy group had significantly increased levels of Scr, BUN, MDA, NF-kB and decreased SOD activity, Sirt1 level, respectively P<0.01; respectively the HE staining of the rats in contrast-induced nephropathy group showed severe tubular injure, medullary congestion, obvious tubular structure damage, epithelial cell brush border loss, vacuolar degeneration, necrosis and protein deposition, and had increased scores of tubular injure, P<0.05. Compared with contrast-induced nephropathy group, curcumin group had obviously decreased levels of Scr, BUN, MDA, NF-kB, and increased SOD activity, Sirt1 level, respectively P<0.05; .and the HE staining of the rats in curcumin group showed lower pathological injure as the contrast-induced nephropathy group, which had decreased scores of tubular injure, P<0.05. CONCLUSIONS Curcumin can obviously alleviate the injure of tubular epithelial cell form iohexol. Protective effects of curcumin to the rats may be through the way of inhibitting oxidative stress and antiapoptotic to complete. Curcumin could probably protect against acute kidney injury through upregulating expression of Sirt1 in experimental rats.
GW27-e0562 The value of high sensitive troponin-i in early diagnosis Of acute myocardial infarction Si-Tuan Nguyen,1,2 Hieu-Hanh Le-Nguyen,2 Lam Hung Hanh1 1 Thongnhat Dongnai General Hospital, Vietnam; 2Medical school, Tantao University, Vietnam OBJECTIVES More than 2.5 million people die of myocardial infarction each year in the world, 25% of them caused by acute myocardial infarction (AMI). The early diagnosis of AMI in the “Golden Period” plays a crucial role in saving the patient’s life. Evaluate the value of Troponin I, a cardiac marker with high sensitivity and specificity for AMI is very necessary. To identify the value of hs-Troponin I in early diagnosis of AMI.
C180
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, VOL. 68, NO. 16, SUPPL S, 2016
METHODS Using cross-sectional study, the research is carried out on 60 patients admitted to hospital, presented with chest pain or chest discomfort in the first 12 hours from May 2015 to March 2016. The patients were withdrawn 2 ml of blood to determine hs-Troponin I levels by Architect i-2000 system. The diagnosis bases on coronary CT angiography in 128-slice CT scanner and ECG. RESULTS The final results showed that AMI was diagnosed in 33 out of 60 cases, which account for 55%. Hs-Troponin I levels of patients in AMI groups were considerably higher than those with other diagnoses. Area under the ROC curve in AMI diagnosis of hs-Troponin I was 0.957. Sensitivity, specificity, positive and negative predictive value of hs-Troponin I at the level of 41.55 ng/L were 91.0%, 89.0%, 91.0% and 89.0%, respectively. CONCLUSIONS The value of high sensitive Troponin-I holds the key position in early diagnosis of AMI.
GW27-e0655 Head to head comparison of two point-of-care platelet function tests used for assessment of on-clopidogrel platelet reactivity in the Chinese acute myocardial infarction patients underwent percutaneous coronary intervention Yi Yao, Jiahui Zhang, Xiaofang Tang, Chen He, Yuanliang Ma, Jingjing XU, Ying Song, Ru Liu, Jinqing Yuan Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College OBJECTIVES Platelet function tests are widely used in clinical practice to guide personalized antiplatelet therapy. In China, thrombelastography (TEG) test has been well accepted in clinics, whereas VerifyNow, so far mainly used for scientific research, has not been used for routine clinical practice. The aim of the study is head to head to compare the two point-of-care platelet function tests and analyze the consistency between the two tests in evaluating on-clopidogrel platelet reactivity in the Chinese acute myocardial infarction(AMI) patients underwent percutaneous coronary Intervention(PCI). METHODS A total of 184 patients were enrolled in the study, onclopidogrel platelet reactivity was assessed three days after PCI by TEG and VerifyNow with adenosine diphosphate(ADP) as agonist. Data were recorded as the percentage inhibition of platelet aggregation (IPA) for TEG and P2Y12 reaction unit (PRU) for VerifyNow. Based on the previous reports, IPA<30% of TEG and PRU>230 of VerifyNow were defined as high on-clopidogrel platelet reactivity; IPA>70% and PRU<178 were defined as low on-clopidogrel platelet reactivity. Correlation and agreement between the two methods were analyzed by spearman correlation coefficient (rs) and kappa value(k), respectively. RESULTS Our results showed that VerifyNow and TEG had a significantly moderate correlation in evaluating platelet reactivity (rs¼ -0.511, p<0.01). A significant although poor agreement (k¼0.225, p<0.01) in identifying high on-clopidogrel platelet reactivity and a significantly moderate agreement in identifying low onclopidogrel platelet reactivity (k¼0.412, p<0.01) were observed between TEG and VerifyNow. By use of TEG as the reference method, the cutoff values for VerifyNow in our study wereidentified as
PRU>205 for high on-clopidogrel platelet reactivity and PRU<169 for low on-clopidogrel platelet reactivity. CONCLUSIONS By comparing VerifyNow to TEG which has been widely used in clinic, our results indicate that VerifyNow could be an attractive alternative to TEG to monitor on-clopidogrel platelet reactivity in the Chinese patients.
GW27-e1199 Difference of carotid artery ultrasound and cardiovascular disease risk factors between rheumatoid arthritis and osteoarthritis Xiujuan Hou, Xiaoping Liu, Wei Zheng, Yuelan Zhu Department of Rheumatology, Beijing University of Traditional Chinese Medicine Subsidiary Dongfang Hospital OBJECTIVES Cardiovascular (CV) morbidity and mortality are increased in rheumatoid arthritis (RA). Chronic systemic inflammation is believed to be an independent risk factor for atherosclerosis. RA and OA for rheumatology outpatients in one of the most common joint disease, We compared carotid intima-media thickness (IMT) of both the common carotid (CCA) and proximal internal carotid (ICA) arteries, and plaque prevalence, between RA and OA participants. METHODS The information of RA and OA patients hospitalized in Department of rheumatism, East Hospital, Beijing University of Chinese Medicine in 2015 were collected, including blood pressure, levels of ESR, CRP, and RA disease activity score (DAS28), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and blood uric acid (BUA); Color Doppler ultrasonography of carotid artery used to observe intima-media thickness (IMT), and plaque formation (bilateral common carotid artery-CCA, internal carotid artery-ICA) and the number of plaques, plaque score -Crouse score). RESULTS We compared 95 rheumatoid arthritis patients to 89 osteroarthritis patients. (1) there was a higher prevalence of hypertension in the RA group; (2)Blood TC, LDL-C and BUA were higher in RA groups than those in OA groups (P < 0.05,;P < 0.01), HDL lever in RA group was lower than that in OA group, (P > 0.05); (3)levels of ESR, CRP and platelet count in RA group were higher than those in OA group (P < 0.01);(4)ICA-IMT was higher in RA patients than controls (1.23 vs 1.06 mm, respectively; p < 0.01), while CCA-IMT did not differ significantly. the odds of plaque were significantly increased in RA participants compared to OA (OR 2.51, 95% CI 1.22-3.92).RA TC, LDL-C and OA groups (P < 0.05, P < 0.01), HDL than in OA group decreased (P > 0.05); (4) in patients with RA than in OA increased (respectively, 1.06 mm, P < 0.01); but CCAIMT had no significant difference; carotid artery plaque incidence in RA was significantly higher in OA (or 2.51, 95% CI 1.22-3.92; LDL-C BUA crouse integral level showed significant positive correlation (P < 0.05). There was significant positive correlation between levels of LDL-C,BUA and Crouse point (P < 0.05). CONCLUSIONS Compared to OA, RA was associated with a higher prevalence and higher severity of atherosclerosis in the ICA. Our data suggest that carotid artery ultrasound measurement could provide evidence for the discovery of atherosclerosis in RA.
C179
expression of cytokines of myofibroblasts, finally transfer the control lentivirus and experimental lentivirus into cells, then evaluated the expression and influence of C/EBPb to myocardial fibrosis, assess the proportion of different subtypes of C/EBPb(LAP, LAP* and LIP) and the ratio between them, assess the degree of fibrosis in each group, detected the expression of downstream proteins cofilin and FLNa, and investigate the relationship of C/EBPb and cofilin, FLNa. In vivo, 24 Lewis rats were randomly divided into the control group, the myosin group, myosinþ control lentivirus group and the myosinþ experimental lentivirus group. The control group did not receive any disposal, other groups were given footpad injection of porcine cardiac myosin 1mg/per rat twice, on the first day and the seventh day respectively. Then import the control lentivirus and experimental lentivirus fused ultrasound microbubble via femoral vein injection combined with local ultrasonic irradiation. The ultrasound indicators of heart were observed, mainly include IVS, IVPW, LVEDD, LVESD, EF, FS and so on. The degree of inflammation in the myocardium was quantified by HE staining, and cardiac fibrosis was quantified by Masson staining. We also measured the expression of a-SMA, C/EBPb, MMP2, MMP9, collagen I, collagen III by immunohistochemistry, the expression of C/EBPb in each group by western blot.
to iohexol group SIRTl protein expression in each group were significantly lower, FOXO3a protein increased, the differences were statistically significant (bP <0.05). IohexolþNA group than the control group, SIRT1, FOXO3a protein expression decreased significantly (aP <0.05); iohexol þ FOXO3a siRNA group than control group, the expression of SIRT1 protein had no significant change (P>0.05), FOXO3a protein decreased expression (aP<0.05), the differences were statistically significant.
RESULTS When C/EBPb was silenced, the myocardial fibrosis, the ratio between LAP and LIP was increased, at the same time the expression of downstream protein cofilin and FLNa were reduced. Compared with the control group, the experimental group has a lower inflammation and fibrosis (P<0.01) level. The expression of a-SMA, C/ EBPb, MMP2, MMP9, collagen I, collagen III were reduced simultaneously in the experimental groups (P<0.05).
OBJECTIVES To observe the effect of curcumin (CUR) intervention on contrast-induced acute kidney injury in experimental rats, and to explore the beneficial effects and the possible mechanism of curcumin intervention on acute kidney injury in experimental rats by the low non-ionic contrast medium, in order to provide more theoretical basis for treating such injury in clinical practice.
CONCLUSIONS Inhibition of the expression of transcription factor C/ EBPb could reduce myocardial fibrosis and inflammation. Inhibition the expression of cofilin and FLNa might be the underlying mechanism. GW27-e0410 Effect of astaxanthin intervention on contrast induced acute kidney injury through the SIRT1/FOXO3a signal Li Wenhua Department of Cardiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou (221002), Jiangsu, China OBJECTIVES To observe the cell proliferation ability, ROS, expressions of SIRT1, FOXO3a in AKI model induced by iodine in HK-2 cells and the effects of astaxanthin on it and its possible mechanisms will be also investigated. Our study will provide a theoretical evidence of astaxanthin on the prevention and treatment of contrast induced acute kidney injury. METHODS HK-2 cells were cultured in DMEM/F12 medium in vitro and then randomly divided into appropriate experimental groups: normal group, dimethyl sulfoxide (DMSO), iohexol group, iohexol þ (1.0, 10.0 mmol/L) astaxanthin group, iohexol þ SIRT1 inhibitors (Nicotinamide, NA) group and iohexol þ siRNA FOXO3a group;when were cultured at 24 hours, cell proliferation ability was tested by CCK-8 method, ROS were detected by flow cytometry, The expression of SIRT1 and FOXO3a were observed using western-blot. RESULTS (1) CCK-8 Results: ① with the increase of concentration of astaxanthin HK-2 cells proliferation has not changed, when AST concentrations > 15mmol/L, cell proliferation was significantly reduced,(aP<0.05), the difference was statistically significant. ② when concentration of the iohexol > 200gI/L, the cell proliferation decreased,(aP<0.05), the difference was statistically significant.③nicotinamide concentration> 20mmol/L, the cell proliferation decreased, (aP <0.05), the difference was statistically significant. (2) Flow cytometry results showed that (after intervention 24 h): compared to the negative control group, the level of ROS in the iohexol group, iohexol þ 1.0mmol/L, 10mmol/L astaxanthin group was significantly increased, the differences were statistically significant (aP<0.05); and between iohexol group and iohexolþ (1.0,10.0mmol/L) astaxanthin group, iohexolþ1.0m mol/L astaxanthin group and iohexol þ 10.0mmol/L astaxanthin group, differences were statistically significant (bP <0.05). (3) Western blot results are shown, iohexol group than normal group SIRTl protein were significantly increased, FOXO3a protein expression decreased, the differences were statistically significant (aP <0.05); iohexolþ(1.0,10.0 mmol/L) astaxanthin group compared
CONCLUSIONS astaxanthin inhibits iohexol induced damage in HK-2, and its possible mechanism is related to decrease expression of endothelial cells SIRT1, and on the upstream of FOXO3a plays against damage induced by iohexol.
GW27-e0413 Effect of Curcumin Intervention on Contrast-induced Kidney Injury in Experimental Rats through Upregulating the Expression of Sirt1 Li Wenhua Department of Cardiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou (221002), Jiangsu, China
METHODS A total of 30 male SD rats were randomly divided into 3 groups: control group, contrast-induced nephropathy group and curcumin group, ten of each group. The rats of curcumin group received intra-gastric curcumin for 5 days, the other two groups were given the corresponding volume of solvent for 5 days. After 48 hours of building model, The renal function was evaluated by serum creatinine(Scr) and urea nitrogen(BUN), the pathological change of the renal tissue was observed by HE staining to evaluate the degree of renal tissue injury, the nephridial oxidative stress indexes of malonadehyde (MDA) level and super oxide dismutase (SOD) activity were measured by the operating kits, then the protein expression of Sirt1 and NF-kB was examined by westernblot. RESULTS Compared with control group, contrast-induced nephropathy group had significantly increased levels of Scr, BUN, MDA, NF-kB and decreased SOD activity, Sirt1 level, respectively P<0.01; respectively the HE staining of the rats in contrast-induced nephropathy group showed severe tubular injure, medullary congestion, obvious tubular structure damage, epithelial cell brush border loss, vacuolar degeneration, necrosis and protein deposition, and had increased scores of tubular injure, P<0.05. Compared with contrast-induced nephropathy group, curcumin group had obviously decreased levels of Scr, BUN, MDA, NF-kB, and increased SOD activity, Sirt1 level, respectively P<0.05; .and the HE staining of the rats in curcumin group showed lower pathological injure as the contrast-induced nephropathy group, which had decreased scores of tubular injure, P<0.05. CONCLUSIONS Curcumin can obviously alleviate the injure of tubular epithelial cell form iohexol. Protective effects of curcumin to the rats may be through the way of inhibitting oxidative stress and antiapoptotic to complete. Curcumin could probably protect against acute kidney injury through upregulating expression of Sirt1 in experimental rats.
GW27-e0562 The value of high sensitive troponin-i in early diagnosis Of acute myocardial infarction Si-Tuan Nguyen,1,2 Hieu-Hanh Le-Nguyen,2 Lam Hung Hanh1 1 Thongnhat Dongnai General Hospital, Vietnam; 2Medical school, Tantao University, Vietnam OBJECTIVES More than 2.5 million people die of myocardial infarction each year in the world, 25% of them caused by acute myocardial infarction (AMI). The early diagnosis of AMI in the “Golden Period” plays a crucial role in saving the patient’s life. Evaluate the value of Troponin I, a cardiac marker with high sensitivity and specificity for AMI is very necessary. To identify the value of hs-Troponin I in early diagnosis of AMI.
C180
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, VOL. 68, NO. 16, SUPPL S, 2016
METHODS Using cross-sectional study, the research is carried out on 60 patients admitted to hospital, presented with chest pain or chest discomfort in the first 12 hours from May 2015 to March 2016. The patients were withdrawn 2 ml of blood to determine hs-Troponin I levels by Architect i-2000 system. The diagnosis bases on coronary CT angiography in 128-slice CT scanner and ECG. RESULTS The final results showed that AMI was diagnosed in 33 out of 60 cases, which account for 55%. Hs-Troponin I levels of patients in AMI groups were considerably higher than those with other diagnoses. Area under the ROC curve in AMI diagnosis of hs-Troponin I was 0.957. Sensitivity, specificity, positive and negative predictive value of hs-Troponin I at the level of 41.55 ng/L were 91.0%, 89.0%, 91.0% and 89.0%, respectively. CONCLUSIONS The value of high sensitive Troponin-I holds the key position in early diagnosis of AMI.
GW27-e0655 Head to head comparison of two point-of-care platelet function tests used for assessment of on-clopidogrel platelet reactivity in the Chinese acute myocardial infarction patients underwent percutaneous coronary intervention Yi Yao, Jiahui Zhang, Xiaofang Tang, Chen He, Yuanliang Ma, Jingjing XU, Ying Song, Ru Liu, Jinqing Yuan Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College OBJECTIVES Platelet function tests are widely used in clinical practice to guide personalized antiplatelet therapy. In China, thrombelastography (TEG) test has been well accepted in clinics, whereas VerifyNow, so far mainly used for scientific research, has not been used for routine clinical practice. The aim of the study is head to head to compare the two point-of-care platelet function tests and analyze the consistency between the two tests in evaluating on-clopidogrel platelet reactivity in the Chinese acute myocardial infarction(AMI) patients underwent percutaneous coronary Intervention(PCI). METHODS A total of 184 patients were enrolled in the study, onclopidogrel platelet reactivity was assessed three days after PCI by TEG and VerifyNow with adenosine diphosphate(ADP) as agonist. Data were recorded as the percentage inhibition of platelet aggregation (IPA) for TEG and P2Y12 reaction unit (PRU) for VerifyNow. Based on the previous reports, IPA<30% of TEG and PRU>230 of VerifyNow were defined as high on-clopidogrel platelet reactivity; IPA>70% and PRU<178 were defined as low on-clopidogrel platelet reactivity. Correlation and agreement between the two methods were analyzed by spearman correlation coefficient (rs) and kappa value(k), respectively. RESULTS Our results showed that VerifyNow and TEG had a significantly moderate correlation in evaluating platelet reactivity (rs¼ -0.511, p<0.01). A significant although poor agreement (k¼0.225, p<0.01) in identifying high on-clopidogrel platelet reactivity and a significantly moderate agreement in identifying low onclopidogrel platelet reactivity (k¼0.412, p<0.01) were observed between TEG and VerifyNow. By use of TEG as the reference method, the cutoff values for VerifyNow in our study wereidentified as
PRU>205 for high on-clopidogrel platelet reactivity and PRU<169 for low on-clopidogrel platelet reactivity. CONCLUSIONS By comparing VerifyNow to TEG which has been widely used in clinic, our results indicate that VerifyNow could be an attractive alternative to TEG to monitor on-clopidogrel platelet reactivity in the Chinese patients.
GW27-e1199 Difference of carotid artery ultrasound and cardiovascular disease risk factors between rheumatoid arthritis and osteoarthritis Xiujuan Hou, Xiaoping Liu, Wei Zheng, Yuelan Zhu Department of Rheumatology, Beijing University of Traditional Chinese Medicine Subsidiary Dongfang Hospital OBJECTIVES Cardiovascular (CV) morbidity and mortality are increased in rheumatoid arthritis (RA). Chronic systemic inflammation is believed to be an independent risk factor for atherosclerosis. RA and OA for rheumatology outpatients in one of the most common joint disease, We compared carotid intima-media thickness (IMT) of both the common carotid (CCA) and proximal internal carotid (ICA) arteries, and plaque prevalence, between RA and OA participants. METHODS The information of RA and OA patients hospitalized in Department of rheumatism, East Hospital, Beijing University of Chinese Medicine in 2015 were collected, including blood pressure, levels of ESR, CRP, and RA disease activity score (DAS28), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and blood uric acid (BUA); Color Doppler ultrasonography of carotid artery used to observe intima-media thickness (IMT), and plaque formation (bilateral common carotid artery-CCA, internal carotid artery-ICA) and the number of plaques, plaque score -Crouse score). RESULTS We compared 95 rheumatoid arthritis patients to 89 osteroarthritis patients. (1) there was a higher prevalence of hypertension in the RA group; (2)Blood TC, LDL-C and BUA were higher in RA groups than those in OA groups (P < 0.05,;P < 0.01), HDL lever in RA group was lower than that in OA group, (P > 0.05); (3)levels of ESR, CRP and platelet count in RA group were higher than those in OA group (P < 0.01);(4)ICA-IMT was higher in RA patients than controls (1.23 vs 1.06 mm, respectively; p < 0.01), while CCA-IMT did not differ significantly. the odds of plaque were significantly increased in RA participants compared to OA (OR 2.51, 95% CI 1.22-3.92).RA TC, LDL-C and OA groups (P < 0.05, P < 0.01), HDL than in OA group decreased (P > 0.05); (4) in patients with RA than in OA increased (respectively, 1.06 mm, P < 0.01); but CCAIMT had no significant difference; carotid artery plaque incidence in RA was significantly higher in OA (or 2.51, 95% CI 1.22-3.92; LDL-C BUA crouse integral level showed significant positive correlation (P < 0.05). There was significant positive correlation between levels of LDL-C,BUA and Crouse point (P < 0.05). CONCLUSIONS Compared to OA, RA was associated with a higher prevalence and higher severity of atherosclerosis in the ICA. Our data suggest that carotid artery ultrasound measurement could provide evidence for the discovery of atherosclerosis in RA.