- Email: [email protected]
Gynogenesis induction in Allium tuberosum L.
Oral presentations / Current Opinion in Biotechnology 24S (2013) S28–S47
in which 35-min-dried flax seedlings with cotyledons were inoculated with 500 l of bacterial solution added to liquid regeneration medium for 20 min.
S41
of BAP and NAA on regeneration and rooting from leaf explant of E. purpurea under in vitro conditions. http://dx.doi.org/10.1016/j.copbio.2013.05.086
http://dx.doi.org/10.1016/j.copbio.2013.05.084 Progressive development of plant biotechnology in Belarus Alexander Kilchevsky Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Belarus E-mail address: [email protected]. The main trend in development of plant biotechnology in Belarus is study on structural and functional organization of plant genomes, development of genetic and cell engineering issues, working out of genomic and cellular biotechnologies for improving the efficiency of plant breeding. The technologies for producing haploids of potato, wheat, triticale and sugar beet were developed in the field of cell engineering. In the field of transgenesis were produced plants as follows: potato resistant to Y-virus, fungal diseases and insects; rape and fiber flax resistant to glyphosate; clover with increased productivity and cranberries with improved taste qualities. The methods of marker-assisted selection (MAS) were developed for 70 genes of resistance and quality of 8 crops (potato, tomato, wheat, apple, rape, soya, barley, flax). The systems of genetic certification were tested on the basis of molecular markers for 10 agricultural crops. More of 170 plant cultivars mentioned above were certified. The results obtained are introduced into practice of breeding institutions of the Republic of Belarus by the innovative center at the Institute of Genetics and Cytology — Republican Centre of Genomic Biotechnologies. http://dx.doi.org/10.1016/j.copbio.2013.05.085 Effect of BAP and NAA plant growth regulators in in vitro regeneration and rooting of Echinacea purpurea (L.) Moench Sam Mokhtarzadeh 1 , Farzad Niyazpour 2 , Farzad Nofouzi 3 , Mohsen Mirzapour 3 , Khalid Mahmood Khawar 3 , Nese Kirimer 1 1 Department of Pharmacognosy, Pharmacy Faculty, University of Anadolu, Eskisehir 26470, Turkey 2 Veterinary Faculty, University of Tabriz, Tabriz, Iran 3 Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara 06110, Turkey E-mail address: [email protected] (S. Mokhtarzadeh).
Eastern purple coneflower or purple coneflower (Echinacea purpurea L.) family Asteraceae, genus Echinacea is an important herbaceous perennial species of flowering plants that grow to 120 cm at maturity. It blooms during spring and summer and is evaluated as an ornamental and medicinal plant. A review of literature suggests development of numerous cultivars for flower quality and medicinal characteristics. It has anti-depressant and immune system stimulating characteristics. Optimum in vitro and ex vitro culture techniques are unknown in Turkey and there is need to optimize these techniques for these plants. It is hoped that development of in vitro protocols for Echinacea sp. can play an important role in establishing of this novel and useful germplasm in Turkey. It will also help in rapid multiplication, biotransformation and genetic transformation for an enhanced phytochemical production. This study reports effects of different concentrations
Study of callus induction and regeneration of Papaver somniferum L. Fereydoon Bondarian 1 , Sepideh Maziar Bahreini 3
Torabi 1 , Mansour
Omidi 2 ,
1 Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran 2 Department of Agronomy & Plant Breeding, University of Tehran, Karaj, Iran 3 Department of Agricultural Biotechnology, Science and Research Branch, Islamic Azad University. Tehran, Iran E-mail address: [email protected] (F. Bondarian).
Papaver somniferum L. (opium poppy) is an herbaceous, annual and diploid plant that is very important because of pharmacological and strategic view in the world. The limitation of this plant farming, hard and law amount extraction of this plant alkaloids cause studying about tissue culture of Papaver somniferum L. for getting high level of these components. This plant has a special group of alkaloids named benzylisoquinolin is due to be secondary metabolites such as morphine, codeine and papaverine is a great value in pharmacy. In this research tissue culture of this plant to achieve the best hormonal levels for callus induction and regeneration examined from seed way culture. Small samples of leaves, roots and hypocotyls of 30–45 days from seedlings, and were transferred to different media with hormonal treatments. Best of hormone treatment for callus induction is 2 mg/l 2,4-D and 0.25 mg/l BAP, 4 mg/l 2,4-D and 0.5 mg/l BAP and best hormonal levels to regeneration includes 1 mg/l BAP and 0.1 mg/l 2,4-D. http://dx.doi.org/10.1016/j.copbio.2013.05.087 Gynogenesis induction in Allium tuberosum L. Fevziye Celebi Toprak, Arzu Kaska, Ali Ramazan Alan Pamukkale University, Plant Genetics and Agricultural Biotechnology Application and Research Center (PAU BIYOM), Kinikli, Denizli 20070, Turkey E-mail address: [email protected] (F.C. Toprak). The present study describes production of plantlets using immature flower buds of Allium tuberosum cultured on various gynogenesis induction media. The gynogenesis induction experiments were carried out by culturing about 21 thousand immature flower buds collected from 30 donor plants during the summer of 2012. In the induction experiments, buds were cultured on BDS, B5 and MS based media with varying amounts of sugar and growth regulators. Gynogenic plantlets started to emerge from cultured buds about 3 months after culture initiation. Gynogenic plantlets were obtained from all media types tested although frequencies of gynogenic plantlet production in various media were significantly different. Induction experiments provided a total of 6100 gynogenic plantlets. Analysis of nuclei samples isolated from gynogenic plants with flow cytometry showed that majority of the regenerants had nuclear DNA contents similar to donor plants. The gynogenic lines were transferred to in vivo successfully. Gynogenic lines are being characterized and compared with donor lines for various features in the greenhouse. http://dx.doi.org/10.1016/j.copbio.2013.05.088
in which 35-min-dried flax seedlings with cotyledons were inoculated with 500 l of bacterial solution added to liquid regeneration medium for 20 min.
S41
of BAP and NAA on regeneration and rooting from leaf explant of E. purpurea under in vitro conditions. http://dx.doi.org/10.1016/j.copbio.2013.05.086
http://dx.doi.org/10.1016/j.copbio.2013.05.084 Progressive development of plant biotechnology in Belarus Alexander Kilchevsky Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Belarus E-mail address: [email protected]. The main trend in development of plant biotechnology in Belarus is study on structural and functional organization of plant genomes, development of genetic and cell engineering issues, working out of genomic and cellular biotechnologies for improving the efficiency of plant breeding. The technologies for producing haploids of potato, wheat, triticale and sugar beet were developed in the field of cell engineering. In the field of transgenesis were produced plants as follows: potato resistant to Y-virus, fungal diseases and insects; rape and fiber flax resistant to glyphosate; clover with increased productivity and cranberries with improved taste qualities. The methods of marker-assisted selection (MAS) were developed for 70 genes of resistance and quality of 8 crops (potato, tomato, wheat, apple, rape, soya, barley, flax). The systems of genetic certification were tested on the basis of molecular markers for 10 agricultural crops. More of 170 plant cultivars mentioned above were certified. The results obtained are introduced into practice of breeding institutions of the Republic of Belarus by the innovative center at the Institute of Genetics and Cytology — Republican Centre of Genomic Biotechnologies. http://dx.doi.org/10.1016/j.copbio.2013.05.085 Effect of BAP and NAA plant growth regulators in in vitro regeneration and rooting of Echinacea purpurea (L.) Moench Sam Mokhtarzadeh 1 , Farzad Niyazpour 2 , Farzad Nofouzi 3 , Mohsen Mirzapour 3 , Khalid Mahmood Khawar 3 , Nese Kirimer 1 1 Department of Pharmacognosy, Pharmacy Faculty, University of Anadolu, Eskisehir 26470, Turkey 2 Veterinary Faculty, University of Tabriz, Tabriz, Iran 3 Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara 06110, Turkey E-mail address: [email protected] (S. Mokhtarzadeh).
Eastern purple coneflower or purple coneflower (Echinacea purpurea L.) family Asteraceae, genus Echinacea is an important herbaceous perennial species of flowering plants that grow to 120 cm at maturity. It blooms during spring and summer and is evaluated as an ornamental and medicinal plant. A review of literature suggests development of numerous cultivars for flower quality and medicinal characteristics. It has anti-depressant and immune system stimulating characteristics. Optimum in vitro and ex vitro culture techniques are unknown in Turkey and there is need to optimize these techniques for these plants. It is hoped that development of in vitro protocols for Echinacea sp. can play an important role in establishing of this novel and useful germplasm in Turkey. It will also help in rapid multiplication, biotransformation and genetic transformation for an enhanced phytochemical production. This study reports effects of different concentrations
Study of callus induction and regeneration of Papaver somniferum L. Fereydoon Bondarian 1 , Sepideh Maziar Bahreini 3
Torabi 1 , Mansour
Omidi 2 ,
1 Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran 2 Department of Agronomy & Plant Breeding, University of Tehran, Karaj, Iran 3 Department of Agricultural Biotechnology, Science and Research Branch, Islamic Azad University. Tehran, Iran E-mail address: [email protected] (F. Bondarian).
Papaver somniferum L. (opium poppy) is an herbaceous, annual and diploid plant that is very important because of pharmacological and strategic view in the world. The limitation of this plant farming, hard and law amount extraction of this plant alkaloids cause studying about tissue culture of Papaver somniferum L. for getting high level of these components. This plant has a special group of alkaloids named benzylisoquinolin is due to be secondary metabolites such as morphine, codeine and papaverine is a great value in pharmacy. In this research tissue culture of this plant to achieve the best hormonal levels for callus induction and regeneration examined from seed way culture. Small samples of leaves, roots and hypocotyls of 30–45 days from seedlings, and were transferred to different media with hormonal treatments. Best of hormone treatment for callus induction is 2 mg/l 2,4-D and 0.25 mg/l BAP, 4 mg/l 2,4-D and 0.5 mg/l BAP and best hormonal levels to regeneration includes 1 mg/l BAP and 0.1 mg/l 2,4-D. http://dx.doi.org/10.1016/j.copbio.2013.05.087 Gynogenesis induction in Allium tuberosum L. Fevziye Celebi Toprak, Arzu Kaska, Ali Ramazan Alan Pamukkale University, Plant Genetics and Agricultural Biotechnology Application and Research Center (PAU BIYOM), Kinikli, Denizli 20070, Turkey E-mail address: [email protected] (F.C. Toprak). The present study describes production of plantlets using immature flower buds of Allium tuberosum cultured on various gynogenesis induction media. The gynogenesis induction experiments were carried out by culturing about 21 thousand immature flower buds collected from 30 donor plants during the summer of 2012. In the induction experiments, buds were cultured on BDS, B5 and MS based media with varying amounts of sugar and growth regulators. Gynogenic plantlets started to emerge from cultured buds about 3 months after culture initiation. Gynogenic plantlets were obtained from all media types tested although frequencies of gynogenic plantlet production in various media were significantly different. Induction experiments provided a total of 6100 gynogenic plantlets. Analysis of nuclei samples isolated from gynogenic plants with flow cytometry showed that majority of the regenerants had nuclear DNA contents similar to donor plants. The gynogenic lines were transferred to in vivo successfully. Gynogenic lines are being characterized and compared with donor lines for various features in the greenhouse. http://dx.doi.org/10.1016/j.copbio.2013.05.088